primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	rps4	1	AAAAATACGCCGTCTGGGT	AAAACCTGACCTGTTGACCC	19	20	47.4	50.0	57.4	57.9	0.0	0.0	1.704549	61	180	246-246	1.000000	66.7
rps4_p2	rps4	2	AAAAATACGCCGTCTGGGT	TTGACCCTTTGGCTTTTCCA	19	20	47.4	45.0	57.4	57.8	0.0	0.0	1.804091	61	180	233-233	1.000000	65.9
rps4_p3	rps4	3	AAAAATACGCCGTCTGGGT	CCTGACCTGTTGACCCTTTG	19	20	47.4	55.0	57.4	58.4	0.0	0.0	2.027976	61	180	242-242	1.000000	64.1
rps4_p4	rps4	4	AAAAATACGCCGTCTGGGT	CTGACCTGTTGACCCTTTGG	19	20	47.4	55.0	57.4	58.4	0.0	0.0	2.027976	61	180	241-241	1.000000	64.1
rps4_p5	rps4	5	AAAAATACGCCGTCTGGGT	GTTGACCCTTTGGCTTTTCC	19	20	47.4	50.0	57.4	57.5	0.0	0.0	2.163487	61	180	234-234	1.000000	63.0
rps4-rps16_p1	rps4-rps16	1	GTAAGTGGGTCGGTTTGGAA	ATTCCTTCAAGTCGCACGTT	20	20	50.0	45.0	57.7	58.1	0.0	0.0	0.380313	634	1185	674-676	1.000000	78.3
rps4-rps16_p2	rps4-rps16	2	GTAAGTGGGTCGGTTTGGAA	AAGTCGCACGTTGCTTTCTA	20	20	50.0	45.0	57.7	58.1	0.0	8.1	0.398590	634	1185	666-668	1.000000	78.1
rps4-rps16_p3	rps4-rps16	3	AAGTGGGTCGGTTTGGAAAT	ATTCCTTCAAGTCGCACGTT	20	20	45.0	45.0	57.6	58.1	0.0	0.0	0.496061	634	1185	672-674	1.000000	77.3
rps4-rps16_p4	rps4-rps16	4	AAGTGGGTCGGTTTGGAAAT	AAGTCGCACGTTGCTTTCTA	20	20	45.0	45.0	57.6	58.1	0.0	8.1	0.514338	634	1185	664-666	1.000000	77.2
rps4-rps16_p5	rps4-rps16	5	AGTAAGTGGGTCGGTTTGGA	ATTCCTTCAAGTCGCACGTT	20	20	50.0	45.0	58.6	58.1	0.0	0.0	0.694296	634	1185	675-677	1.000000	75.7
rps16-trnK-UUU_p1	rps16-trnK-UUU	1	GCTCAACCTACGGAAACTGT	TCTACCAACGGTATGGACGA	20	20	50.0	50.0	57.8	57.9	0.0	10.6	0.292589	2337	2985	826-829	1.000000	78.2
rps16-trnK-UUU_p2	rps16-trnK-UUU	2	GCTCAACCTACGGAAACTGT	AAAGCCGAGTACTCTACCGT	20	20	50.0	50.0	57.8	58.2	0.0	20.3	0.333738	2337	2985	761-764	1.000000	78.0
rps16-trnK-UUU_p3	rps16-trnK-UUU	3	GCTCAACCTACGGAAACTGT	AAGCCGAGTACTCTACCGTT	20	20	50.0	50.0	57.8	58.2	0.0	20.3	0.333738	2337	2985	760-763	1.000000	78.0
rps16-trnK-UUU_p4	rps16-trnK-UUU	4	CCTTGAAAAAGGCGCTCAAC	TCTACCAACGGTATGGACGA	20	20	50.0	50.0	58.2	57.9	7.6	10.6	0.356314	2337	2985	839-842	1.000000	77.7
rps16-trnK-UUU_p5	rps16-trnK-UUU	5	CTTGAAAAAGGCGCTCAACC	TCTACCAACGGTATGGACGA	20	20	50.0	50.0	58.2	57.9	7.6	10.6	0.356314	2337	2985	838-841	1.000000	77.7
ndhJ-ndhK_p1	ndhJ-ndhK	1	GCCAATCCTCGGGCTTTATT	CGCCATCTACTTCAGAGATCC	20	21	50.0	52.4	58.3	57.7	0.0	0.0	1.603911	11129	11263	324-324	1.000000	67.7
ndhJ-ndhK_p2	ndhJ-ndhK	2	CAAGCAGACAAACGACCTTG	CGCCATCTACTTCAGAGATCC	20	21	50.0	52.4	57.6	57.7	0.0	0.0	1.686035	11129	11263	238-238	1.000000	67.2
ndhJ-ndhK_p3	ndhJ-ndhK	3	CCAAGCAGACAAACGACCTT	CGCCATCTACTTCAGAGATCC	20	21	50.0	52.4	58.7	57.7	0.0	0.0	1.987324	11129	11263	239-239	1.000000	64.8
ndhJ-ndhK_p4	ndhJ-ndhK	4	GCCAATCCTCGGGCTTTATT	GCCATCTACTTCAGAGATCCC	20	21	50.0	52.4	58.3	57.3	0.0	0.0	1.983880	11129	11263	323-323	1.000000	64.6
ndhJ-ndhK_p5	ndhJ-ndhK	5	CAAGCAGACAAACGACCTTG	GCCATCTACTTCAGAGATCCC	20	21	50.0	52.4	57.6	57.3	0.0	0.0	2.066004	11129	11263	237-237	1.000000	64.1
atpH-atpI_p1	atpH-atpI	1	TCCAGGTCCAATAGAAGCCA	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	58.0	57.9	0.0	21.3	0.100384	17369	18483	1215-1220	1.000000	79.7
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.1	57.9	0.0	21.3	0.169579	17369	18483	1170-1175	1.000000	79.2
atpH-atpI_p3	atpH-atpI	3	GATACCTTCTACGGCTTGGC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.1	57.9	0.0	21.3	0.198632	17369	18483	1257-1262	1.000000	78.9
atpH-atpI_p4	atpH-atpI	4	CGATACCTTCTACGGCTTGG	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	57.9	57.9	0.0	21.3	0.201463	17369	18483	1258-1263	1.000000	78.9
atpH-atpI_p5	atpH-atpI	5	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	17369	18483	1188-1193	1.000000	78.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.746530	30746	31828	1159-1171	1.000000	75.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GTTCTTCCGTCAATCCCTGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.844663	30746	31828	1236-1248	1.000000	74.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGTTCTTCCGTCAATCCCTG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.2	58.1	0.0	0.0	0.852292	30746	31828	1237-1249	1.000000	74.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTCCCTCATTTCCATCCCG	GCAAAGGGTTTCGGGTTTTT	20	20	50.0	45.0	57.3	57.7	0.0	0.0	0.983538	30746	31828	1195-1207	1.000000	73.1
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ATTCCCTCATTTCCATCCCG	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	57.3	57.7	0.0	0.0	0.989985	30746	31828	1177-1189	1.000000	73.1
petN-psbM_p1	petN-psbM	1	TCGGGGGCATAATTCACATG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.0	57.6	0.0	0.0	1.427569	32436	33229	972-980	1.000000	70.0
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	32436	33229	887-895	1.000000	67.5
petN-psbM_p3	petN-psbM	3	AAGTCTTGCTTGGTCTGCTT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.6	57.6	0.0	0.0	1.762433	32436	33229	944-952	1.000000	67.3
petN-psbM_p4	petN-psbM	4	AGTCTTGCTTGGTCTGCTTT	TGCTACTGCACTGTTCATTCT	20	21	45.0	42.9	57.6	57.6	0.0	0.0	1.762433	32436	33229	943-951	1.000000	67.3
petN-psbM_p5	petN-psbM	5	GGGGGCATAATTCACATGGA	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.787488	32436	33229	970-978	1.000000	67.1
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGATGT	ATCAAGCTGCCGAGTATTGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.085081	46473	47214	920-920	1.000000	79.5
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGATGT	CAGAGGCTTGGTTCGATCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.143264	46473	47214	935-935	1.000000	79.0
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGATGT	CGATCAAGCTGCCGAGTATT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.186031	46473	47214	922-922	1.000000	78.7
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGATGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.235712	46473	47214	853-853	1.000000	78.3
psaA-ycf3_p5	psaA-ycf3	5	GAGAAATGACCTGGTCTGGC	ATCAAGCTGCCGAGTATTGG	20	20	55.0	50.0	58.3	58.0	26.0	0.0	0.296963	46473	47214	938-938	1.000000	77.8
rbcL-accD_p1	rbcL-accD	1	GCTGCTTGTGAGGTATGGAA	GGTCGAATACCCCCTAGTGA	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.191548	56646	57383	887-888	1.000000	78.6
rbcL-accD_p2	rbcL-accD	2	GCTGCTTGTGAGGTATGGAA	ATCGAAGTGAACCGCCATTT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.292644	56646	57383	824-825	1.000000	77.9
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	ATTTACTTAACCCGCACCCG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.372332	56646	57383	868-869	1.000000	77.2
rbcL-accD_p4	rbcL-accD	4	GCTGCTTGTGAGGTATGGAA	AGTGAACCGCCATTTTTCCA	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.415354	56646	57383	819-820	1.000000	76.9
rbcL-accD_p5	rbcL-accD	5	GCTGCTTGTGAGGTATGGAA	CACCCGTGTTCTAACTCCTC	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.479268	56646	57383	854-855	1.000000	76.4
accD-psaI_p1	accD-psaI	1	TCAGCTTCACGGTTTCTGTC	GCCATTGCAATTGCTGGAAA	20	20	50.0	45.0	58.1	58.2	0.0	32.1	0.323176	58827	59373	655-659	1.000000	77.8
accD-psaI_p2	accD-psaI	2	TTCAGCTTCACGGTTTCTGT	GCCATTGCAATTGCTGGAAA	20	20	45.0	45.0	57.7	58.2	0.0	32.1	0.514624	58827	59373	656-660	1.000000	76.2
accD-psaI_p3	accD-psaI	3	GCTTCACGGTTTCTGTCCTT	GCCATTGCAATTGCTGGAAA	20	20	50.0	45.0	58.4	58.2	0.0	32.1	0.594711	58827	59373	652-656	1.000000	75.6
accD-psaI_p4	accD-psaI	4	TCAGCTTCACGGTTTCTGTC	GAAGCCATTGCAATTGCTGG	20	20	50.0	50.0	58.1	58.6	0.0	31.8	0.768436	58827	59373	658-662	1.000000	74.2
accD-psaI_p5	accD-psaI	5	TCAGCTTCACGGTTTCTGTC	GGAAGCCATTGCAATTGCTG	20	20	50.0	50.0	58.1	58.6	0.0	31.8	0.768436	58827	59373	659-663	1.000000	74.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.536253	64138	65477	1484-1497	1.000000	76.5
psbE-petL_p2	psbE-petL	2	ACGAATAACCAACCCGCAAT	CCGCCAGTAGAAAACCGAAA	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.655939	64138	65477	1493-1506	1.000000	75.5
psbE-petL_p3	psbE-petL	3	TGCTGACGAATAACCAACCC	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.668844	64138	65477	1498-1511	1.000000	75.4
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	57.8	58.5	0.0	0.0	0.672270	64138	65477	1492-1505	1.000000	75.4
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	CCGCCAGTAGAAAACCGAAA	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.722211	64138	65477	1486-1499	1.000000	75.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCAAGTGTATTGGGCAGGAA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	56.7	58.0	0.0	0.0	1.272074	113744	114664	973-973	1.000000	70.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCAAGTGTATTGGGCAGGAA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	56.7	58.0	0.0	0.0	1.273445	113744	114664	974-974	1.000000	70.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TCAAGTGTATTGGGCAGGAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.388521	113744	114664	983-983	1.000000	69.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCAAGTGTATTGGGCAGGAA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	56.7	57.8	0.0	0.0	1.460539	113744	114664	982-982	1.000000	68.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCAAGTGTATTGGGCAGGAA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	56.7	57.8	0.0	0.0	1.460539	113744	114664	981-981	1.000000	68.7
ccsA_p1	ccsA	1	TCAGATCTCCATTCCATTCTTGG	TCAAAAACTCGTACAAACTTTCGT	23	24	43.5	33.3	58.0	57.8	0.0	0.0	7.213176	114860	115825	1098-1100	1.000000	40.1
ccsA_p2	ccsA	2	TCAGATCTCCATTCCATTCTTGG	CAAAAACTCGTACAAACTTTCGT	23	23	43.5	34.8	58.0	56.5	0.0	0.0	7.522457	114860	115825	1097-1099	1.000000	40.1
ccsA_p3	ccsA	3	TCAGATCTCCATTCCATTCTTGG	AAACTCGTACAAACTTTCGT	23	20	43.5	35.0	58.0	53.5	0.0	0.0	7.594844	114860	115825	1094-1096	1.000000	40.1
ccsA_p4	ccsA	4	TCAGATCTCCATTCCATTCTTGG	AACTCGTACAAACTTTCGTT	23	20	43.5	35.0	58.0	53.5	0.0	0.0	7.594844	114860	115825	1093-1095	1.000000	40.1
ccsA_p5	ccsA	5	CAGATCTCCATTCCATTCTTGG	TCAAAAACTCGTACAAACTTTCGT	22	24	45.5	33.3	56.6	57.8	0.0	0.0	7.617976	114860	115825	1097-1099	1.000000	40.1
ccsA-ndhD_p1	ccsA-ndhD	1	GGGCTGCATAGTTATGGTTCA	TCCGGATTTCGTTCTCTCAC	21	20	47.6	50.0	58.1	57.4	0.5	0.0	1.718381	115826	116065	352-354	1.000000	67.8
ccsA-ndhD_p2	ccsA-ndhD	2	GGGCTGCATAGTTATGGTTCA	CCGGATTTCGTTCTCTCACT	21	20	47.6	50.0	58.1	57.3	0.5	0.0	1.725610	115826	116065	351-353	1.000000	67.8
ccsA-ndhD_p3	ccsA-ndhD	3	AGGGCTGCATAGTTATGGTTC	TCCGGATTTCGTTCTCTCAC	21	20	47.6	50.0	57.8	57.4	0.5	0.0	1.864027	115826	116065	353-355	1.000000	66.7
ccsA-ndhD_p4	ccsA-ndhD	4	AGGGCTGCATAGTTATGGTTC	CCGGATTTCGTTCTCTCACT	21	20	47.6	50.0	57.8	57.3	0.5	0.0	1.871256	115826	116065	352-354	1.000000	66.6
ccsA-ndhD_p5	ccsA-ndhD	5	AGGGCTGCATAGTTATGGTT	TCCGGATTTCGTTCTCTCAC	20	20	45.0	50.0	56.6	57.4	0.5	0.0	2.077555	115826	116065	353-355	1.000000	65.0
ndhE_p1	ndhE	1	TCTGCTCATGAAAGAAGGCC	TCGAGATTGATGAGGAGTTGT	20	21	50.0	42.9	57.9	56.5	0.0	0.0	2.646141	118220	118525	447-448	1.000000	59.0
ndhE_p2	ndhE	2	CTGCTCATGAAAGAAGGCCA	TCGAGATTGATGAGGAGTTGT	20	21	50.0	42.9	58.2	56.5	5.0	0.0	2.696061	118220	118525	446-447	1.000000	58.6
ndhE_p3	ndhE	3	TGCTCATGAAAGAAGGCCAA	TCGAGATTGATGAGGAGTTGT	20	21	45.0	42.9	57.7	56.5	5.0	0.0	2.817549	118220	118525	445-446	1.000000	57.6
ndhE_p4	ndhE	4	TCTGCTCATGAAAGAAGGCC	TCGAGATTGATGAGGAGTTGTT	20	22	50.0	40.9	57.9	57.2	0.0	0.0	2.928216	118220	118525	447-448	1.000000	56.8
ndhE_p5	ndhE	5	CTGCTCATGAAAGAAGGCCA	TCGAGATTGATGAGGAGTTGTT	20	22	50.0	40.9	58.2	57.2	5.0	0.0	2.978136	118220	118525	446-447	1.000000	56.4
ndhE-ndhG_p1	ndhE-ndhG	1	AAGACACATAAGCGCTCGAA	TGCTATGGCTCGTCAGTACT	20	20	45.0	50.0	57.6	58.2	12.1	1.7	0.599820	118526	118813	399-412	1.000000	78.6
ndhE-ndhG_p2	ndhE-ndhG	2	AGACACATAAGCGCTCGAAC	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.4	58.2	12.1	1.7	0.605884	118526	118813	398-411	1.000000	78.5
ndhE-ndhG_p3	ndhE-ndhG	3	CAAGACACATAAGCGCTCGA	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.4	58.2	12.1	1.7	0.607019	118526	118813	400-413	1.000000	78.5
ndhE-ndhG_p4	ndhE-ndhG	4	TCAAGACACATAAGCGCTCG	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.4	58.2	12.1	1.7	0.607019	118526	118813	401-414	1.000000	78.5
ndhE-ndhG_p5	ndhE-ndhG	5	GCTCAAGACACATAAGCGCT	TGCTATGGCTCGTCAGTACT	20	20	50.0	50.0	58.6	58.2	2.6	1.7	0.872288	118526	118813	403-416	1.000000	76.3
ndhA_p1	ndhA	1	ATGAATCCGGTTACCATGGG	TGGGAGAAGTTGATCGTTGA	20	20	50.0	45.0	57.3	56.5	18.1	0.0	2.169552	120443	122540	2218-2218	1.000000	63.0
ndhA_p2	ndhA	2	AATCCGGTTACCATGGGGAA	TGGGAGAAGTTGATCGTTGA	20	20	50.0	45.0	58.7	56.5	18.1	0.0	2.211677	120443	122540	2215-2215	1.000000	62.7
ndhA_p3	ndhA	3	ATGAATCCGGTTACCATGGG	TGGGAGAAGTTGATCGTTGAA	20	21	50.0	42.9	57.3	57.2	18.1	0.0	2.419354	120443	122540	2218-2218	1.000000	61.0
ndhA_p4	ndhA	4	AATCCGGTTACCATGGGGAA	TGGGAGAAGTTGATCGTTGAA	20	21	50.0	42.9	58.7	57.2	18.1	0.0	2.461480	120443	122540	2215-2215	1.000000	60.7
ndhA_p5	ndhA	5	CGGTTACCATGGGGAACATA	TGGGAGAAGTTGATCGTTGA	20	20	50.0	45.0	57.0	56.5	25.0	0.0	2.525461	120443	122540	2211-2211	1.000000	60.2
ycf1_p1	ycf1	1	TGAAATATTCCGTGTGTTCCA	ACGGATAATTCCAATGATGAAACA	21	24	38.1	33.3	55.5	57.0	0.0	0.0	8.500659	126509	126658	296-296	1.000000	41.1
ycf1_p2	ycf1	2	TGAAATATTCCGTGTGTTCCAA	ACGGATAATTCCAATGATGAAACA	22	24	36.4	33.3	56.3	57.0	0.0	0.0	8.741136	126509	126658	296-296	1.000000	41.1
ycf1_p3	ycf1	3	TGAAATATTCCGTGTGTTCCA	AACGGATAATTCCAATGATGAAACA	21	25	38.1	32.0	55.5	57.6	0.0	0.0	8.885860	126509	126658	297-297	1.000000	41.1
ycf1_p4	ycf1	4	TGAAATATTCCGTGTGTTCCA	ACGGATAATTCCAATGATGAAACAA	21	25	38.1	32.0	55.5	57.6	0.0	0.0	8.885860	126509	126658	296-296	1.000000	41.1
ycf1_p5	ycf1	5	ATGAAATATTCCGTGTGTTCCA	ACGGATAATTCCAATGATGAAACA	22	24	36.4	33.3	56.1	57.0	0.0	0.0	8.953148	126509	126658	297-297	1.000000	41.1