primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	CGGGGGTATGGACGAATTTT	AATTTGCACGACAGAAGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.065745	4058	5938	1951-2052	1.000000	84.7
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	TCTTACCAACTCTACCGGGG	AATTTGCACGACAGAAGGGT	20	20	55.0	45.0	58.1	58.0	6.3	0.0	0.162115	4058	5938	1966-2067	1.000000	83.9
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	CGGGGGTATGGACGAATTTT	TCCACGTTTTGTCAGGGAAA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.468753	4058	5938	1992-2093	1.000000	81.4
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	CGGGGGTATGGACGAATTTT	TTCCACGTTTTGTCAGGGAA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.468753	4058	5938	1993-2094	1.000000	81.4
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	TCTTACCAACTCTACCGGGG	TCCACGTTTTGTCAGGGAAA	20	20	55.0	45.0	58.1	57.6	6.3	0.0	0.565122	4058	5938	2007-2108	1.000000	80.6
atpA-atpF_p1	atpA-atpF	1	CATCGGGGCGAATAGTTACC	AAAGAGCTCTGCGAACTCTG	20	20	55.0	50.0	58.2	57.9	0.0	19.1	0.285407	13103	13219	251-255	1.000000	79.6
atpA-atpF_p2	atpA-atpF	2	CATCGGGGCGAATAGTTACC	CAAAGAGCTCTGCGAACTCT	20	20	55.0	50.0	58.2	57.9	0.0	19.1	0.285407	13103	13219	252-256	1.000000	79.6
atpA-atpF_p3	atpA-atpF	3	CATCGGGGCGAATAGTTACC	CGATCATCATATCGGCCTCC	20	20	55.0	55.0	58.2	57.8	0.0	0.0	0.423901	13103	13219	193-197	1.000000	79.1
atpA-atpF_p4	atpA-atpF	4	CATCGGGGCGAATAGTTACC	GAGCTCTGCGAACTCTGAAT	20	20	55.0	50.0	58.2	57.7	0.0	6.4	0.500097	13103	13219	248-252	1.000000	77.9
atpA-atpF_p5	atpA-atpF	5	CATCGGGGCGAATAGTTACC	ATATCGGCCTCCTTAGAGCC	20	20	55.0	55.0	58.2	58.4	0.0	0.0	0.645211	13103	13219	185-189	1.000000	77.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TGAACAATGCAACAGCAAGA	TTAGAGTACCGCCCTGTCAA	20	20	40.0	50.0	56.5	58.1	0.0	0.0	1.613026	29120	30463	1430-1442	1.000000	69.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TGAACAATGCAACAGCAAGA	ATTGGTTAGAGTACCGCCCT	20	20	40.0	50.0	56.5	58.1	0.0	0.0	1.665025	29120	30463	1435-1447	1.000000	68.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TGAACAATGCAACAGCAAGAA	TTAGAGTACCGCCCTGTCAA	21	20	38.1	50.0	57.2	58.1	0.0	0.0	1.869963	29120	30463	1430-1442	1.000000	66.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TGAACAATGCAACAGCAAGA	TTACTGCGGATTAGTTGGCG	20	20	40.0	50.0	56.5	58.4	0.0	0.0	1.893168	29120	30463	1480-1492	1.000000	66.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TGAACAATGCAACAGCAAGAA	ATTGGTTAGAGTACCGCCCT	21	20	38.1	50.0	57.2	58.1	0.0	0.0	1.921963	29120	30463	1435-1447	1.000000	66.5
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCCGCTACAATTGTGAACGT	ACGGAAAGAGAGGGATTCGA	20	20	45.0	50.0	57.8	58.2	21.4	0.0	0.309182	44441	45085	788-792	1.000000	78.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCCGCTACAATTGTGAACGT	TTCCAATGCTACGCCTTGAA	20	20	45.0	45.0	57.8	57.8	21.4	5.6	0.350498	44441	45085	738-742	1.000000	78.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CTCTTTTTCCCCTGAAGCCA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.444176	44441	45085	831-835	1.000000	77.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TGCCTCTTTTTCCCCTGAAG	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.444176	44441	45085	834-838	1.000000	77.3
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CTCTTTTTCCCCTGAAGCCA	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.7	57.8	0.0	5.6	0.485492	44441	45085	781-785	1.000000	77.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CAAGTGCGATGCTCTAACCT	CGTTTCCATACCAAGGCTCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.123686	46555	46957	498-501	1.000000	80.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CAAGTGCGATGCTCTAACCT	ACGTTTCCATACCAAGGCTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.125161	46555	46957	499-502	1.000000	80.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	CAAGTGCGATGCTCTAACCT	ATTCGATCAAGTCCGTAGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.136852	46555	46957	478-481	1.000000	80.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CAAGTGCGATGCTCTAACCT	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.166639	46555	46957	466-469	1.000000	80.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	CAAGTGCGATGCTCTAACCT	GGGTTCCCTGGATTTGGAAG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.163103	46555	46957	531-534	1.000000	80.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	AGGACTGAAAATCCTCGTGC	TTGGCCTTTACGCAAAGACT	20	20	50.0	45.0	57.9	58.0	0.0	9.8	0.124945	47915	48426	618-619	1.000000	80.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AGGACTGAAAATCCTCGTGC	GTTGGCCTTTACGCAAAGAC	20	20	50.0	50.0	57.9	58.0	0.0	9.8	0.146887	47915	48426	619-620	1.000000	80.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AGGACTGAAAATCCTCGTGC	TGAGAGTTGGATTGGTTGGC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.190905	47915	48426	633-634	1.000000	80.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AGGACTGAAAATCCTCGTGC	AGTTGGATTGGTTGGCCTTT	20	20	50.0	45.0	57.9	57.9	0.0	3.3	0.202244	47915	48426	629-630	1.000000	79.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GACTGAAAATCCTCGTGCCA	TTGGCCTTTACGCAAAGACT	20	20	50.0	45.0	58.2	58.0	0.0	9.8	0.215930	47915	48426	616-617	1.000000	79.8
rbcL-trnR-CCG_p1	rbcL-trnR-CCG	1	GTGATCCGTGAAGCTAGCAA	AACCATGTGCTCTGATCCAC	20	20	50.0	50.0	58.0	57.9	18.8	0.0	0.129946	55855	56404	683-684	1.000000	80.8
rbcL-trnR-CCG_p2	rbcL-trnR-CCG	2	GTGATCCGTGAAGCTAGCAA	TCGGGCTAGAAGGGATTTGA	20	20	50.0	50.0	58.0	58.1	18.8	0.0	0.136016	55855	56404	724-725	1.000000	80.7
rbcL-trnR-CCG_p3	rbcL-trnR-CCG	3	GTGATCCGTGAAGCTAGCAA	CTCGGGCTAGAAGGGATTTG	20	20	50.0	55.0	58.0	57.8	18.8	0.0	0.256864	55855	56404	725-726	1.000000	79.7
rbcL-trnR-CCG_p4	rbcL-trnR-CCG	4	GTGATCCGTGAAGCTAGCAA	ATTGAATAGGGCTCGGGCTA	20	20	50.0	50.0	58.0	58.3	18.8	7.5	0.280559	55855	56404	736-737	1.000000	79.5
rbcL-trnR-CCG_p5	rbcL-trnR-CCG	5	GTGATCCGTGAAGCTAGCAA	GATCCACTGAGCTACCAACC	20	20	50.0	55.0	58.0	57.7	18.8	0.0	0.327675	55855	56404	670-671	1.000000	79.3
psbL-psbF_p1	psbL-psbF	1	TAACAACCCCCAGTAGAGGC	AATGCAGTTCATCCAGCGAT	20	20	55.0	45.0	58.7	58.0	0.0	0.0	0.766062	64536	64569	125-125	1.000000	74.5
psbL-psbF_p2	psbL-psbF	2	TAACAACCCCCAGTAGAGGC	CGTTCACGGACTAGCTGTAC	20	20	55.0	55.0	58.7	58.1	0.0	0.0	0.825150	64536	64569	176-176	1.000000	73.9
psbL-psbF_p3	psbL-psbF	3	TAACAACCCCCAGTAGAGGC	AGTTAGATGGTTGGCCGTTC	20	20	55.0	50.0	58.7	57.9	0.0	0.0	0.827042	64536	64569	191-191	1.000000	73.8
psbL-psbF_p4	psbL-psbF	4	TAACAACCCCCAGTAGAGGC	CAGTTAGATGGTTGGCCGTT	20	20	55.0	50.0	58.7	58.2	0.0	0.0	0.896401	64536	64569	192-192	1.000000	73.2
psbL-psbF_p5	psbL-psbF	5	TAACAACCCCCAGTAGAGGC	TTAGATGGTTGGCCGTTCAC	20	20	55.0	50.0	58.7	58.2	0.0	0.0	0.901543	64536	64569	189-189	1.000000	73.2
psbE-petL_p1	psbE-petL	1	ATAATGTCAGCAAAAGCGCG	GGTGGAAATTAGCAGAGCCA	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.307245	64951	65899	1044-1048	1.000000	79.1
psbE-petL_p2	psbE-petL	2	TTCTCCCGTATTCCCAGACA	GGTGGAAATTAGCAGAGCCA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.370051	64951	65899	1024-1028	1.000000	78.7
psbE-petL_p3	psbE-petL	3	TCTCCCGTATTCCCAGACAT	GGTGGAAATTAGCAGAGCCA	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.600586	64951	65899	1023-1027	1.000000	76.8
psbE-petL_p4	psbE-petL	4	ATAATGTCAGCAAAAGCGCG	AGGTGGAAATTAGCAGAGCC	20	20	45.0	50.0	57.8	57.6	0.0	0.0	0.605316	64951	65899	1045-1049	1.000000	76.8
psbE-petL_p5	psbE-petL	5	TTCTCCCGTATTCCCAGACA	AGGTGGAAATTAGCAGAGCC	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.668123	64951	65899	1025-1029	1.000000	76.3
rpl2-rps19_p1	rpl2-rps19	1	GTGATGCTTCGATCCTTCGT	CCGGGACCAGGTCACTATTA	20	20	50.0	55.0	58.1	58.2	0.0	20.2	0.287747	71789	71844	199-199	1.000000	78.1
rpl2-rps19_p2	rpl2-rps19	2	CTATAGCGCGCTTGGAAGAA	CCGGGACCAGGTCACTATTA	20	20	50.0	55.0	58.1	58.2	41.0	20.2	0.362700	71789	71844	243-243	1.000000	77.4
rpl2-rps19_p3	rpl2-rps19	3	TGATGCTTCGATCCTTCGTC	CCGGGACCAGGTCACTATTA	20	20	50.0	55.0	57.8	58.2	0.0	20.2	0.425480	71789	71844	198-198	1.000000	77.0
rpl2-rps19_p4	rpl2-rps19	4	AGTGATGCTTCGATCCTTCG	CCGGGACCAGGTCACTATTA	20	20	50.0	55.0	57.8	58.2	0.0	20.2	0.431549	71789	71844	200-200	1.000000	76.9
rpl2-rps19_p5	rpl2-rps19	5	CGATCCTTCGTCGACGTAAG	CCGGGACCAGGTCACTATTA	20	20	55.0	55.0	58.3	58.2	34.4	20.2	0.487811	71789	71844	190-190	1.000000	76.5
psbB-clpP_p1	psbB-clpP	1	AGCCCAACCGGAAACTAAAG	TGAGGTACTTTTGGAACGCC	20	20	50.0	50.0	58.1	58.1	9.5	0.0	0.207476	83453	84108	785-787	1.000000	79.2
psbB-clpP_p2	psbB-clpP	2	CCCAACCGGAAACTAAAGCT	TGAGGTACTTTTGGAACGCC	20	20	50.0	50.0	58.1	58.1	9.5	0.0	0.207476	83453	84108	783-785	1.000000	79.2
psbB-clpP_p3	psbB-clpP	3	GGTATGGACGCGATACCAAG	TGAGGTACTTTTGGAACGCC	20	20	55.0	50.0	58.2	58.1	23.4	0.0	0.333544	83453	84108	713-715	1.000000	78.2
psbB-clpP_p4	psbB-clpP	4	GTATGGACGCGATACCAAGG	TGAGGTACTTTTGGAACGCC	20	20	55.0	50.0	58.2	58.1	15.9	0.0	0.333544	83453	84108	712-714	1.000000	78.2
psbB-clpP_p5	psbB-clpP	5	CGATACCAAGGCAAACCCAT	TGAGGTACTTTTGGAACGCC	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.350406	83453	84108	703-705	1.000000	78.1
clpP-ycf1_p1	clpP-ycf1	1	ATGAGCAGGATTTTGCACGA	TAACCACGCGTATACCAACG	20	20	45.0	50.0	58.2	58.1	0.5	13.4	0.268153	84781	85412	704-713	1.000000	78.9
clpP-ycf1_p2	clpP-ycf1	2	AATTGGCAAGAGAAGGACGG	TAACCACGCGTATACCAACG	20	20	50.0	50.0	58.2	58.1	6.0	13.4	0.266859	84781	85412	762-771	1.000000	78.8
clpP-ycf1_p3	clpP-ycf1	3	ATGAGCAGGATTTTGCACGA	CAGCGGTAATGTGGAAAGGA	20	20	45.0	50.0	58.2	57.9	0.5	0.0	0.282860	84781	85412	767-776	1.000000	78.7
clpP-ycf1_p4	clpP-ycf1	4	AATTGGCAAGAGAAGGACGG	CAGCGGTAATGTGGAAAGGA	20	20	50.0	50.0	58.2	57.9	6.0	0.0	0.281565	84781	85412	825-834	1.000000	78.6
clpP-ycf1_p5	clpP-ycf1	5	AATTGGCAAGAGAAGGACGG	TCAGCGGTAATGTGGAAAGG	20	20	50.0	50.0	58.2	57.9	6.0	0.0	0.281565	84781	85412	826-835	1.000000	78.6
ycf1-rps15_p1	ycf1-rps15	1	CACCATTTTCCTGCTTTGCA	GCAAATGCTGGGTAAGTCCT	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.690357	92970	93763	954-958	1.000000	76.2
ycf1-rps15_p2	ycf1-rps15	2	TGCACCATTTTCCTGCTTTG	GCAAATGCTGGGTAAGTCCT	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.690357	92970	93763	956-960	1.000000	76.1
ycf1-rps15_p3	ycf1-rps15	3	ACTTTGCACCATTTTCCTGC	GCAAATGCTGGGTAAGTCCT	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.692099	92970	93763	960-964	1.000000	76.1
ycf1-rps15_p4	ycf1-rps15	4	CACCATTTTCCTGCTTTGCA	TGCAAATGCTGGGTAAGTCC	20	20	45.0	50.0	57.5	58.5	0.0	7.0	0.985057	92970	93763	955-959	1.000000	73.8
ycf1-rps15_p5	ycf1-rps15	5	TGCACCATTTTCCTGCTTTG	TGCAAATGCTGGGTAAGTCC	20	20	45.0	50.0	57.5	58.5	0.0	7.0	0.985057	92970	93763	957-961	1.000000	73.8
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	CGCTGATGAGCACATTGAAC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	57.8	57.7	4.7	7.8	0.464916	113189	113828	775-779	1.000000	77.5
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	CGCTGATGAGCACATTGAAC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	57.8	58.3	4.7	0.0	0.476037	113189	113828	797-801	1.000000	77.4
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	113189	113828	724-728	1.000000	77.4
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.496177	113189	113828	746-750	1.000000	77.3
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	CGCTGATGAGCACATTGAAC	AAGTCGTAACAAGGTAGCCG	20	20	50.0	50.0	57.8	57.6	4.7	0.0	0.528602	113189	113828	784-788	1.000000	77.0
rrn16-trnV-GAC_p1	rrn16-trnV-GAC	1	ATGTGTTAAGCATGCCACCA	GACGTGGTGATAGTCATCGG	20	20	45.0	55.0	58.1	57.9	15.7	0.0	0.199134	115304	116752	1536-1539	1.000000	80.2
rrn16-trnV-GAC_p2	rrn16-trnV-GAC	2	CCGACTTGCATGTGTTAAGC	GACGTGGTGATAGTCATCGG	20	20	50.0	55.0	57.8	57.9	10.4	0.0	0.368427	115304	116752	1545-1548	1.000000	78.9
rrn16-trnV-GAC_p3	rrn16-trnV-GAC	3	CATGTGTTAAGCATGCCACC	GACGTGGTGATAGTCATCGG	20	20	50.0	55.0	57.7	57.9	15.7	0.0	0.403817	115304	116752	1537-1540	1.000000	78.6
rrn16-trnV-GAC_p4	rrn16-trnV-GAC	4	GCAGGTTCTTACGCGTTACT	GACGTGGTGATAGTCATCGG	20	20	50.0	55.0	58.3	57.9	20.8	0.0	0.414673	115304	116752	1598-1601	1.000000	78.4
rrn16-trnV-GAC_p5	rrn16-trnV-GAC	5	TTCTTACGCGTTACTCACCC	GACGTGGTGATAGTCATCGG	20	20	50.0	55.0	57.6	57.9	20.8	0.0	0.473619	115304	116752	1593-1596	1.000000	78.0
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	GCAATTGCTCAGGAGACCTT	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.2	58.0	23.2	5.8	0.176250	122013	122281	319-328	1.000000	82.1
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	GCAATTGCTCAGGAGACCTT	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.2	58.0	23.2	5.8	0.177621	122013	122281	317-326	1.000000	82.1
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	GCAATTGCTCAGGAGACCTT	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	58.2	58.2	23.2	0.0	0.365086	122013	122281	314-323	1.000000	80.6
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	GCAATTGCTCAGGAGACCTT	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	58.2	57.7	23.2	5.8	0.441789	122013	122281	318-327	1.000000	79.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	GCAATTGCTCAGGAGACCTT	TCGCGTGTCTACCATTTCAC	20	20	50.0	50.0	58.2	58.3	23.2	5.8	0.463164	122013	122281	316-325	1.000000	79.8
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CGGTTCTTGTCTCGGTTCAT	TACCATTCAGCCATGGATGC	20	20	50.0	50.0	57.9	58.0	0.0	25.4	0.094813	129365	129911	587-687	1.000000	81.4
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	CGGTTCTTGTCTCGGTTCAT	CCGCGACTTCCCAATTATGA	20	20	50.0	50.0	57.9	58.1	0.0	8.2	0.132182	129365	129911	619-719	1.000000	81.0
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CGGTTCTTGTCTCGGTTCAT	CCACAGGCTATTGGAAGACC	20	20	50.0	55.0	57.9	58.0	0.0	8.4	0.124409	129365	129911	660-760	1.000000	80.9
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CGGTTCTTGTCTCGGTTCAT	ACGACCCGACTTAGCTATCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.128730	129365	129911	1148-1248	1.000000	80.5
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CGGTTCTTGTCTCGGTTCAT	GAATGGCTCATGCTAACCCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.137423	129365	129911	1125-1225	1.000000	80.5