primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	AAGGAGCAATAACGCCTTCT	ATTTGTGTGCTTGGGAGTCC	20	20	45.0	50.0	57.2	58.4	0.0	0.0	1.168586	411	1510	1212-1250	1.000000	73.3
psbA_p2	psbA	2	AGGAGCAATAACGCCTTCTT	ATTTGTGTGCTTGGGAGTCC	20	20	45.0	50.0	57.2	58.4	0.0	0.0	1.168586	411	1510	1211-1249	1.000000	73.3
psbA_p3	psbA	3	AAGGAGCAATAACGCCTTCTT	ATTTGTGTGCTTGGGAGTCC	21	20	42.9	50.0	57.9	58.4	0.0	0.0	1.452451	411	1510	1212-1250	1.000000	71.0
psbA_p4	psbA	4	AAGGAGCAATAACGCCTTCT	TTTGTGTGCTTGGGAGTCC	20	19	45.0	52.6	57.2	57.9	0.0	0.0	1.899256	411	1510	1211-1249	1.000000	67.4
psbA_p5	psbA	5	AGGAGCAATAACGCCTTCTT	TTTGTGTGCTTGGGAGTCC	20	19	45.0	52.6	57.2	57.9	0.0	0.0	1.899256	411	1510	1210-1248	1.000000	67.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	4277	5105	964-966	1.000000	79.2
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.2	58.0	20.3	9.2	0.173044	4277	5105	963-965	1.000000	79.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCCTAGCCGCACTTAAAAGC	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.3	58.0	0.0	9.2	0.270461	4277	5105	979-981	1.000000	78.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	TTTCCTTGAAAGGGGTGCTC	20	20	50.0	50.0	58.3	58.0	5.3	9.2	0.287430	4277	5105	973-975	1.000000	78.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4277	5105	950-952	1.000000	77.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6212	7655	1517-1517	1.000000	78.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6212	7655	1531-1531	1.000000	76.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AAAAATCGAAATGGGGCGTG	20	20	45.0	45.0	58.1	57.6	8.1	0.0	0.501493	6212	7655	1576-1576	1.000000	76.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	GGATCGTGTCCTTCAAGTCG	AAAAATCGAAATGGGGCGTG	20	20	55.0	45.0	58.4	57.6	2.9	0.0	0.732022	6212	7655	1590-1590	1.000000	74.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6212	7655	1521-1521	1.000000	73.8
trnQ-UUG-psbk_p1	trnQ-UUG-psbk	1	GATATGCTCTGGGACGGAAG	AAAAAGCATAGGCCTCGGG	20	19	55.0	52.6	57.6	57.8	0.0	9.7	1.638427	7728	8056	506-506	1.000000	67.1
trnQ-UUG-psbk_p2	trnQ-UUG-psbk	2	GACCAAAACCCGTTGCCTTA	AAAAAGCATAGGCCTCGGG	20	19	50.0	52.6	58.7	57.8	0.0	9.7	1.870676	7728	8056	464-464	1.000000	65.2
trnQ-UUG-psbk_p3	trnQ-UUG-psbk	3	AATGGATATGCTCTGGGACG	AAAAAGCATAGGCCTCGGG	20	19	50.0	52.6	57.2	57.8	0.0	9.7	2.038509	7728	8056	510-510	1.000000	63.8
trnQ-UUG-psbk_p4	trnQ-UUG-psbk	4	GAATAGCGGGACCAAAACCC	AAAAAGCATAGGCCTCGGG	20	19	55.0	52.6	58.9	57.8	0.0	9.7	2.090673	7728	8056	473-473	1.000000	63.4
trnQ-UUG-psbk_p5	trnQ-UUG-psbk	5	ATATGCTCTGGGACGGAAGG	AAAAAGCATAGGCCTCGGG	20	19	55.0	52.6	58.9	57.8	0.0	9.7	2.139371	7728	8056	505-505	1.000000	63.0
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACGGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	28819	30011	1257-1289	1.000000	74.0
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACGGCCT	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.033465	28819	30011	1255-1287	1.000000	74.0
petN-psbM_p3	petN-psbM	3	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACGGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	28819	30011	1251-1283	1.000000	73.5
petN-psbM_p4	petN-psbM	4	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACGGCCTTTCT	20	21	50.0	47.6	58.0	57.9	0.0	0.0	1.102712	28819	30011	1253-1285	1.000000	73.4
petN-psbM_p5	petN-psbM	5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACGGCCT	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.108380	28819	30011	1254-1286	1.000000	73.4
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	CTCCTAGGCGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.1	57.9	9.4	0.0	0.183275	46707	47099	483-505	1.000000	82.3
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	CTCCTAGGCGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	20	20	50.0	50.0	58.1	57.8	9.4	0.0	0.291028	46707	47099	451-473	1.000000	81.7
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	CTCCTAGGCGGCGTATTTTT	TGGATGTTATATGAGCCCGC	20	20	50.0	50.0	58.1	57.5	9.4	0.0	0.523230	46707	47099	507-529	1.000000	79.4
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	CTCCTAGGCGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	20	20	50.0	55.0	58.1	57.5	9.4	7.6	0.551043	46707	47099	491-513	1.000000	79.3
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	CTCCTAGGCGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	20	20	50.0	50.0	58.1	57.5	9.4	0.0	0.573370	46707	47099	481-503	1.000000	79.2
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	48994	49682	831-834	1.000000	81.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	48994	49682	795-798	1.000000	81.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	20	20	55.0	45.0	58.0	58.2	10.3	0.0	0.194095	48994	49682	866-869	1.000000	80.7
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.223436	48994	49682	819-822	1.000000	80.6
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCTTGAAACGT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.243485	48994	49682	830-833	1.000000	80.4
atpB-rbcL_p1	atpB-rbcL	1	CCCCGGAACCAGAAGTAGTA	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	58.1	58.0	0.7	0.0	1.160636	55494	56282	848-872	1.000000	73.2
atpB-rbcL_p2	atpB-rbcL	2	GCCGATGATTTGGACAATGC	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.2	58.0	0.0	0.0	1.173273	55494	56282	898-922	1.000000	73.0
atpB-rbcL_p3	atpB-rbcL	3	CCCCGGAACCAGAAGTAGTA	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	58.1	58.1	0.7	0.0	1.193924	55494	56282	847-871	1.000000	73.0
atpB-rbcL_p4	atpB-rbcL	4	GCCGATGATTTGGACAATGC	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.2	58.1	0.0	0.0	1.206561	55494	56282	897-921	1.000000	72.7
atpB-rbcL_p5	atpB-rbcL	5	AGGCTACATCTAGTACCGGG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	57.7	58.0	6.3	0.0	1.306857	55494	56282	917-941	1.000000	71.9
accD-psaI_p1	accD-psaI	1	GACCTAATCGTACCGCGTAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.2	58.0	4.9	26.9	0.782840	59936	60698	875-932	1.000000	82.0
accD-psaI_p2	accD-psaI	2	GACCTAATCGTACCGCGTAA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.2	57.9	4.9	26.8	0.859374	59936	60698	881-938	1.000000	81.3
accD-psaI_p3	accD-psaI	3	ATTTCAGTTCCACGCTTTCG	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.0	58.0	0.0	26.9	0.996446	59936	60698	828-885	1.000000	80.5
accD-psaI_p4	accD-psaI	4	ATTTCAGTTCCACGCTTTCG	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	57.0	57.9	0.0	26.8	1.072980	59936	60698	834-891	1.000000	79.9
accD-psaI_p5	accD-psaI	5	GACCTAATCGTACCGCGTAA	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	57.2	57.5	4.9	8.9	1.208132	59936	60698	864-921	1.000000	78.6
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.064968	95341	95925	622-645	1.000000	82.4
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.0	57.9	5.8	29.4	0.066338	95341	95925	620-643	1.000000	82.4
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	TACCATTTCACCACCAAGGC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.1	57.9	0.0	29.4	0.139265	95341	95925	610-633	1.000000	81.8
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	58.2	57.9	0.0	29.4	0.253803	95341	95925	617-640	1.000000	80.9
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	20	20	50.0	50.0	57.7	57.9	5.8	29.4	0.330507	95341	95925	621-644	1.000000	80.3
rrn16_p1	rrn16	1	CGAATCCGCTTTGTCTACGA	GTGTGAAGCGGTGTCAAAC	20	19	50.0	52.6	58.1	57.5	0.0	0.0	1.622681	101685	103175	1593-1593	1.000000	67.0
rrn16_p2	rrn16	2	CGAATCCGCTTTGTCTACGA	GGTGTGAAGCGGTGTCAAA	20	19	50.0	52.6	58.1	58.6	0.0	0.0	1.696000	101685	103175	1594-1594	1.000000	66.4
rrn16_p3	rrn16	3	CGAATCCGCTTTGTCTACGA	TGTGAAGCGGTGTCAAACC	20	19	50.0	52.6	58.1	58.6	0.0	0.0	1.696000	101685	103175	1592-1592	1.000000	66.4
rrn16_p4	rrn16	4	GAACTCCGGGCGAATATGAA	GTGTGAAGCGGTGTCAAAC	20	19	50.0	52.6	57.8	57.5	0.0	0.0	1.761125	101685	103175	1654-1654	1.000000	65.9
rrn16_p5	rrn16	5	AACTCCGGGCGAATATGAAG	GTGTGAAGCGGTGTCAAAC	20	19	50.0	52.6	57.8	57.5	0.0	0.0	1.767319	101685	103175	1653-1653	1.000000	65.9
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	103176	103477	389-396	1.000000	79.8
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.7	57.8	7.8	0.0	0.462074	103176	103477	421-428	1.000000	79.7
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.496177	103176	103477	411-418	1.000000	79.5
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	58.3	57.8	0.0	0.0	0.473195	103176	103477	443-450	1.000000	79.4
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	AAGTCGTAACAAGGTAGCCG	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	57.6	57.8	0.0	0.0	0.525760	103176	103477	430-437	1.000000	79.1
ndhF-rpl32_p1	ndhF-rpl32	1	AAAAGAAGAAGTCCCAGCCC	TAAGGCTGTCCAATACCCCT	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.574758	113645	114521	1011-1050	1.000000	79.1
ndhF-rpl32_p2	ndhF-rpl32	2	AAGAAGAAGTCCCAGCCCTA	TAAGGCTGTCCAATACCCCT	20	20	50.0	50.0	57.7	57.7	0.0	0.0	0.624401	113645	114521	1009-1048	1.000000	78.7
ndhF-rpl32_p3	ndhF-rpl32	3	AAAGAAGAAGTCCCAGCCCT	TAAGGCTGTCCAATACCCCT	20	20	50.0	50.0	58.5	57.7	0.0	0.0	0.829949	113645	114521	1010-1049	1.000000	77.1
ndhF-rpl32_p4	ndhF-rpl32	4	AGAAGAAGTCCCAGCCCTAT	TAAGGCTGTCCAATACCCCT	20	20	50.0	50.0	57.4	57.7	0.0	0.0	0.858080	113645	114521	1008-1047	1.000000	76.9
ndhF-rpl32_p5	ndhF-rpl32	5	AAAAGAAGAAGTCCCAGCCC	AAGGCTGTCCAATACCCCTT	20	20	50.0	50.0	57.7	58.6	0.0	0.0	0.911535	113645	114521	1010-1049	1.000000	76.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGGTATTGGACAGCCTTA	TATCATGCCCTTTTCGGACG	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.332204	114684	115389	1110-1242	1.000000	88.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGGTATTGGACAGCCTTA	AGCCTTGGGTAAAAACAGCA	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.360962	114684	115389	1306-1438	1.000000	87.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGGTATTGGACAGCCTTA	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.369251	114684	115389	1316-1448	1.000000	87.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGGTATTGGACAGCCTTA	TGAAGTAGCAAAGCCTTGGG	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.370608	114684	115389	1317-1449	1.000000	87.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGGTATTGGACAGCCTTA	GCCTTGGGTAAAAACAGCAC	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.429224	114684	115389	1305-1437	1.000000	87.0
rps15-ycf1_p1	rps15-ycf1	1	TCCAAATGTGAAGTAAGCCTCT	CGGCAGTCGTTTCAGTATGT	22	20	40.9	50.0	57.4	58.3	0.0	0.0	2.930758	124656	125041	540-557	1.000000	59.5
rps15-ycf1_p2	rps15-ycf1	2	CCAAATGTGAAGTAAGCCTCTC	CGGCAGTCGTTTCAGTATGT	22	20	45.5	50.0	57.0	58.3	0.0	0.0	3.244079	124656	125041	539-556	1.000000	57.0
rps15-ycf1_p3	rps15-ycf1	3	TTCCAAATGTGAAGTAAGCCTCT	CGGCAGTCGTTTCAGTATGT	23	20	39.1	50.0	58.0	58.3	0.0	0.0	3.304449	124656	125041	541-558	1.000000	56.5
rps15-ycf1_p4	rps15-ycf1	4	TCCAAATGTGAAGTAAGCCTC	CGGCAGTCGTTTCAGTATGT	21	20	42.9	50.0	55.9	58.3	0.0	0.0	3.413774	124656	125041	540-557	1.000000	55.6
rps15-ycf1_p5	rps15-ycf1	5	CCAAATGTGAAGTAAGCCTCT	CGGCAGTCGTTTCAGTATGT	21	20	42.9	50.0	55.9	58.3	0.0	0.0	3.424449	124656	125041	539-556	1.000000	55.5
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	20	20	50.0	55.0	58.2	57.7	0.0	7.8	0.485056	137478	137779	389-396	1.000000	79.8
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	20	20	55.0	55.0	57.8	57.7	0.0	7.8	0.462074	137478	137779	421-428	1.000000	79.7
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.496177	137478	137779	411-418	1.000000	79.5
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	20	20	55.0	50.0	57.8	58.3	0.0	0.0	0.473195	137478	137779	443-450	1.000000	79.4
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	20	20	55.0	50.0	57.8	57.6	0.0	0.0	0.525760	137478	137779	430-437	1.000000	79.1
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	145030	145614	622-645	1.000000	82.4
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	145030	145614	620-643	1.000000	82.4
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	20	20	50.0	50.0	57.9	58.1	29.4	0.0	0.139265	145030	145614	610-633	1.000000	81.8
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	29.4	0.0	0.253803	145030	145614	617-640	1.000000	80.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	57.9	57.7	29.4	5.8	0.330507	145030	145614	621-644	1.000000	80.3