primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG AGACCTAGCTGCTATCGAAG 20 20 55.0 50.0 58.1 56.2 0.0 10.2 1.881489 76 417 400-401 1.000000 67.1 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA AGACCTAGCTGCTATCGAAG 20 20 50.0 50.0 57.9 56.2 0.0 10.2 1.885851 76 417 414-415 1.000000 67.0 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC AGACCTAGCTGCTATCGAAG 20 20 50.0 50.0 57.9 56.2 0.0 10.2 1.887358 76 417 413-414 1.000000 67.0 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACAATCCACTGCCTTGATCC AGACCTAGCTGCTATCGAAG 20 20 50.0 50.0 57.9 56.2 0.0 10.2 1.887358 76 417 415-416 1.000000 67.0 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 GCCTTGATCCACTTGGCTAC AGACCTAGCTGCTATCGAAG 20 20 55.0 50.0 58.6 56.2 0.0 10.2 2.369046 76 417 405-406 1.000000 63.2 matK_p1 matK 1 AGTGGAGATCCCATTTTGATCTT AACCCCCGAATCTTCTACCT 23 20 39.1 50.0 57.5 57.7 6.2 0.0 3.728578 2017 3561 1663-1663 1.000000 50.4 matK_p2 matK 2 AGTGGAGATCCCATTTTGATCTT ACCCCCGAATCTTCTACCTT 23 20 39.1 50.0 57.5 57.7 6.2 0.0 3.728578 2017 3561 1662-1662 1.000000 50.4 matK_p3 matK 3 GTGGAGATCCCATTTTGATCTT AACCCCCGAATCTTCTACCT 22 20 40.9 50.0 56.1 57.7 6.2 0.0 4.157136 2017 3561 1662-1662 1.000000 47.0 matK_p4 matK 4 GTGGAGATCCCATTTTGATCTT ACCCCCGAATCTTCTACCTT 22 20 40.9 50.0 56.1 57.7 6.2 0.0 4.157136 2017 3561 1661-1661 1.000000 47.0 matK_p5 matK 5 AGTGGAGATCCCATTTTGATCTTT ACCCCCGAATCTTCTACCTT 24 20 37.5 50.0 58.2 57.7 6.2 0.0 4.429944 2017 3561 1662-1662 1.000000 44.8 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CGCACTTAAAAGCCGAGTAC TCCTTGAAAAAGGGGCTCAA 20 20 50.0 45.0 57.2 57.2 0.0 4.7 1.547887 4293 4824 671-675 1.000000 69.2 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CGCACTTAAAAGCCGAGTAC TTCCTTGAAAAAGGGGCTCA 20 20 50.0 45.0 57.2 57.2 0.0 4.7 1.547887 4293 4824 672-676 1.000000 69.2 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CGCACTTAAAAGCCGAGTAC CCTTGAAAAAGGGGCTCAAC 20 20 50.0 50.0 57.2 57.2 0.0 0.0 1.602257 4293 4824 670-674 1.000000 68.7 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CGCACTTAAAAGCCGAGTAC CTTGAAAAAGGGGCTCAACC 20 20 50.0 50.0 57.2 57.2 0.0 0.0 1.602257 4293 4824 669-673 1.000000 68.7 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 AGCCGAGTACTCTACCATTGA TCCTTGAAAAAGGGGCTCAA 21 20 47.6 45.0 58.0 57.2 20.3 4.7 1.779993 4293 4824 661-665 1.000000 67.3 rps16_p1 rps16 1 ATATACAAGCCGCTACCCCC GCAGCTGAACCGATGACTAT 20 20 55.0 50.0 59.0 57.8 0.0 20.4 1.249272 4825 5959 1242-1244 1.000000 70.3 rps16_p2 rps16 2 TATACAAGCCGCTACCCCC GCAGCTGAACCGATGACTAT 19 20 57.9 50.0 58.6 57.8 0.0 20.4 1.786934 4825 5959 1241-1243 1.000000 66.0 rps16_p3 rps16 3 TATATACAAGCCGCTACCCC GCAGCTGAACCGATGACTAT 20 20 50.0 50.0 56.3 57.8 0.0 20.4 1.958424 4825 5959 1243-1245 1.000000 64.6 rps16_p4 rps16 4 ATATACAAGCCGCTACCCCC AAGTGTAGATTGCGACGTCT 20 20 55.0 45.0 59.0 57.0 0.0 14.7 2.029751 4825 5959 1266-1268 1.000000 64.0 rps16_p5 rps16 5 TATATACAAGCCGCTACCCCC GCAGCTGAACCGATGACTAT 21 20 52.4 50.0 58.8 57.8 0.0 20.4 2.049239 4825 5959 1243-1245 1.000000 63.9 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.0 58.1 0.0 16.9 0.081289 5960 6990 1099-1122 1.000000 80.6 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 GTCTTTCAAGTCGCACGTTG TTAGACATCAACTTGGGGCG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.113138 5960 6990 1160-1183 1.000000 80.3 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 GTCTTTCAAGTCGCACGTTG GTCTCGCTATTCGGAGGTTC 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.127751 5960 6990 1112-1135 1.000000 80.3 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 5960 6990 1092-1115 1.000000 79.6 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 AAGTCGCACGTTGCTTTCTA TTAGACATCAACTTGGGGCG 20 20 45.0 50.0 58.1 57.9 8.1 0.0 0.247274 5960 6990 1153-1176 1.000000 79.3 rpoC2_p1 rpoC2 1 CCCATTCGAAAACAATTGGATGA GGCAAACGGTTTGATTCGAC 23 20 39.1 50.0 57.9 58.3 13.5 31.7 3.388945 17956 18075 282-282 1.000000 54.0 rpoC2_p2 rpoC2 2 CCCATTCGAAAACAATTGGATGA GCAAACGGTTTGATTCGACA 23 20 39.1 45.0 57.9 57.6 13.5 31.7 3.463702 17956 18075 281-281 1.000000 53.4 rpoC2_p3 rpoC2 3 ATCAACGATTGGAATCCCTT GGCAAACGGTTTGATTCGAC 20 20 40.0 50.0 54.7 58.3 0.0 31.7 3.632466 17956 18075 249-249 1.000000 52.2 rpoC2_p4 rpoC2 4 ATCAACGATTGGAATCCCTT GCAAACGGTTTGATTCGACA 20 20 40.0 45.0 54.7 57.6 0.0 31.7 3.707223 17956 18075 248-248 1.000000 51.6 rpoC2_p5 rpoC2 5 CCCATTCGAAAACAATTGGATGA TCTAGTCAAGAAGCTCGTGC 23 20 39.1 50.0 57.9 57.4 13.5 0.1 3.709326 17956 18075 320-320 1.000000 51.3 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 GACAAACCCCGATCGATGAA CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.0 58.1 18.7 0.0 0.100670 26931 28104 1326-1331 1.000000 79.9 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 GACAAACCCCGATCGATGAA TTTTCGTGATTCAGGCGACA 20 20 50.0 45.0 58.0 58.1 18.7 0.0 0.146154 26931 28104 1347-1352 1.000000 79.5 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 GACATTGCCTCGTTTGCATC CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.181891 26931 28104 1260-1265 1.000000 79.2 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 GACATTGCCTCGTTTGCATC TTTTCGTGATTCAGGCGACA 20 20 50.0 45.0 58.1 58.1 0.0 0.0 0.227375 26931 28104 1281-1286 1.000000 78.8 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 TAGACATTGCCTCGTTTGCA CCGGATTTGAACTGGGGAAA 20 20 45.0 50.0 57.8 58.1 0.0 0.0 0.259522 26931 28104 1262-1267 1.000000 78.6 petN-psbM_p1 petN-psbM 1 ACTCGTAGTGTGGGGAAGAA TCCGACCGCCTTTCTACTTA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.167258 28433 29463 1113-1117 1.000000 79.5 petN-psbM_p2 petN-psbM 2 TCGTAGTGTGGGGAAGAAGT TCCGACCGCCTTTCTACTTA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.167258 28433 29463 1111-1115 1.000000 79.5 petN-psbM_p3 petN-psbM 3 ACTCGTAGTGTGGGGAAGAA TAGTTCCGACCGCCTTTCTA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.167258 28433 29463 1117-1121 1.000000 79.4 petN-psbM_p4 petN-psbM 4 TCGTAGTGTGGGGAAGAAGT TAGTTCCGACCGCCTTTCTA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.167258 28433 29463 1115-1119 1.000000 79.4 petN-psbM_p5 petN-psbM 5 CGTAGTGTGGGGAAGAAGTG TCCGACCGCCTTTCTACTTA 20 20 55.0 50.0 57.9 58.2 0.0 0.0 0.242174 28433 29463 1110-1114 1.000000 78.9 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 20 20 55.0 45.0 58.1 58.2 7.6 0.0 0.310137 31151 32461 1455-1461 1.000000 78.1 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 20 20 55.0 55.0 58.1 57.7 7.6 0.0 0.320185 31151 32461 1439-1445 1.000000 78.0 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.332292 31151 32461 1458-1464 1.000000 77.9 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 TCAGTGGTAGAGTAACGCCA CCCTCCGTAACCAATCATCC 20 20 50.0 55.0 58.1 57.7 0.0 0.0 0.342339 31151 32461 1442-1448 1.000000 77.9 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GTGGTAGAGTAACGCCATGG GCCAGACCAGCCTACAAAAA 20 20 55.0 50.0 58.1 58.4 7.6 0.0 0.447909 31151 32461 1468-1474 1.000000 77.0 ycf3_p1 ycf3 1 TGGCTCTTTCCACAAAACAA CCACGAGAGGGAATGAGAAG 20 20 40.0 55.0 56.0 57.4 0.0 0.0 2.617208 42071 44029 2036-2041 1.000000 59.4 ycf3_p2 ycf3 2 TTGGCTCTTTCCACAAAACA CCACGAGAGGGAATGAGAAG 20 20 40.0 55.0 56.0 57.4 0.0 0.0 2.617208 42071 44029 2037-2042 1.000000 59.4 ycf3_p3 ycf3 3 TGGCTCTTTCCACAAAACAAAG CCACGAGAGGGAATGAGAAG 22 20 40.9 55.0 57.9 57.4 0.0 0.0 2.715489 42071 44029 2036-2041 1.000000 58.6 ycf3_p4 ycf3 4 TCTTTGATTGGCTCTTTCCACA CCACGAGAGGGAATGAGAAG 22 20 40.9 55.0 58.2 57.4 0.0 0.0 2.762023 42071 44029 2044-2049 1.000000 58.3 ycf3_p5 ycf3 5 TGGCTCTTTCCACAAAACAAA CCACGAGAGGGAATGAGAAG 21 20 38.1 55.0 56.8 57.4 0.0 0.0 2.842024 42071 44029 2036-2041 1.000000 57.6 ycf3-trnS-GGA_p1 ycf3-trnS-GGA 1 TGCCTCCTTTTCTCCTGAAG AAGGTTCGGAAAGAGAGGGA 20 20 50.0 50.0 57.1 58.0 0.0 0.0 0.892745 44030 44865 1032-1035 1.000000 73.3 ycf3-trnS-GGA_p2 ycf3-trnS-GGA 2 GCCTCCTTTTCTCCTGAAGT AAGGTTCGGAAAGAGAGGGA 20 20 50.0 50.0 57.1 58.0 0.0 0.0 0.894737 44030 44865 1031-1034 1.000000 73.3 ycf3-trnS-GGA_p3 ycf3-trnS-GGA 3 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 20 20 50.0 50.0 57.1 58.1 0.0 0.0 0.961472 44030 44865 1006-1009 1.000000 72.8 ycf3-trnS-GGA_p4 ycf3-trnS-GGA 4 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 20 20 50.0 50.0 57.1 58.1 0.0 0.0 0.963464 44030 44865 1005-1008 1.000000 72.8 ycf3-trnS-GGA_p5 ycf3-trnS-GGA 5 TGCCTCCTTTTCTCCTGAAG TCGGAAAGAGAGGGATTCGA 20 20 50.0 50.0 57.1 57.9 0.0 0.0 1.006432 44030 44865 1027-1030 1.000000 72.4 rps4-trnT-UGU_p1 rps4-trnT-UGU 1 TCTAAGATCACTCCCGGCTT CTCAGAGGTTAGAGCATCGC 20 20 50.0 55.0 57.8 57.9 0.0 0.0 0.293461 45778 46248 631-635 1.000000 78.8 rps4-trnT-UGU_p2 rps4-trnT-UGU 2 TTCTAAGATCACTCCCGGCT CTCAGAGGTTAGAGCATCGC 20 20 50.0 55.0 57.8 57.9 0.0 0.0 0.293461 45778 46248 632-636 1.000000 78.8 rps4-trnT-UGU_p3 rps4-trnT-UGU 3 TCTAAGATCACTCCCGGCTT ATGGTCATCGGTTCGATTCC 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.401214 45778 46248 599-603 1.000000 78.0 rps4-trnT-UGU_p4 rps4-trnT-UGU 4 TTCTAAGATCACTCCCGGCT ATGGTCATCGGTTCGATTCC 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.401214 45778 46248 600-604 1.000000 78.0 rps4-trnT-UGU_p5 rps4-trnT-UGU 5 GTTAGTCCTGGTAAAGCCCC CTCAGAGGTTAGAGCATCGC 20 20 55.0 55.0 57.6 57.9 0.0 0.0 0.547734 45778 46248 597-601 1.000000 76.8 accD-psaI_p1 accD-psaI 1 TAAGCTCCACGCCTTCTTTC CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.9 58.0 0.0 26.9 0.121766 58760 59481 826-838 1.000000 80.7 accD-psaI_p2 accD-psaI 2 CTCCACGCCTTCTTTCCTTT CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.1 58.0 0.0 26.9 0.129443 58760 59481 822-834 1.000000 80.6 accD-psaI_p3 accD-psaI 3 CGACTTAATCGTACCGCGTA CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 57.6 58.0 4.9 26.9 0.394402 58760 59481 877-889 1.000000 78.4 accD-psaI_p4 accD-psaI 4 TAAGCTCCACGCCTTCTTTC AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 57.9 57.9 0.0 26.8 0.198299 58760 59481 832-844 0.900000 75.9 accD-psaI_p5 accD-psaI 5 CTCCACGCCTTCTTTCCTTT AAGAAGCCATTGCAATTGCC 20 20 50.0 45.0 58.1 57.9 0.0 26.8 0.205977 58760 59481 828-840 0.900000 75.9 petA-psbJ_p1 petA-psbJ 1 GGCGTCTGTTATTTTGGCAC TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 58.0 57.5 0.0 0.0 0.512339 63127 64178 1155-1166 1.000000 78.3 petA-psbJ_p2 petA-psbJ 2 GGCGTCTGTTATTTTGGCAC CCGGATTAGGTTCATCCCTG 20 20 50.0 55.0 58.0 57.4 0.0 0.0 0.573642 63127 64178 1154-1165 1.000000 77.8 petA-psbJ_p3 petA-psbJ 3 GAGAAGGTTCAATTGGCCGA TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 58.2 57.5 5.4 0.0 0.668311 63127 64178 1103-1114 1.000000 77.2 petA-psbJ_p4 petA-psbJ 4 TCGAGAAGGTTCAATTGGCC TCCGGATTAGGTTCATCCCT 20 20 50.0 50.0 58.2 57.5 5.4 0.0 0.668311 63127 64178 1105-1116 1.000000 77.2 petA-psbJ_p5 petA-psbJ 5 GAGAAGGTTCAATTGGCCGA CCGGATTAGGTTCATCCCTG 20 20 50.0 55.0 58.2 57.4 5.4 0.0 0.729614 63127 64178 1102-1113 1.000000 76.7 rpl20-rps12_p1 rpl20-rps12 1 ACCCTCGAAAACTTGATGCA CGCCGAGGAACATGTACTAG 20 20 45.0 55.0 57.7 57.9 0.0 0.0 0.391468 68826 69636 927-931 1.000000 77.4 rpl20-rps12_p2 rpl20-rps12 2 CGGGCTATATATCCGCGTTT CGCCGAGGAACATGTACTAG 20 20 50.0 55.0 57.7 57.9 4.9 0.0 0.410077 68826 69636 880-884 1.000000 77.3 rpl20-rps12_p3 rpl20-rps12 3 ACCCTCGAAAACTTGATGCA ACTAGGGTGTATGTGCGACT 20 20 45.0 50.0 57.7 58.2 0.0 0.0 0.421700 68826 69636 912-916 1.000000 77.2 rpl20-rps12_p4 rpl20-rps12 4 CGGGCTATATATCCGCGTTT ACTAGGGTGTATGTGCGACT 20 20 50.0 50.0 57.7 58.2 4.9 0.0 0.440309 68826 69636 865-869 1.000000 77.0 rpl20-rps12_p5 rpl20-rps12 5 ACCCTCGAAAACTTGATGCA AATACAAGACAGCCCGTTCG 20 20 45.0 50.0 57.7 58.3 0.0 0.0 0.548341 68826 69636 987-991 1.000000 76.1 ndhD_p1 ndhD 1 TTGTGCAACGAACCCTTTTG TTACCGACAAAATCCGTGCT 20 20 45.0 45.0 57.7 57.8 0.0 0.0 0.458678 114194 115723 1649-1650 1.000000 76.8 ndhD_p2 ndhD 2 TCATTGTGCAACGAACCCTT TTACCGACAAAATCCGTGCT 20 20 45.0 45.0 58.3 57.8 0.0 0.0 0.486496 114194 115723 1652-1653 1.000000 76.5 ndhD_p3 ndhD 3 TTGTGCAACGAACCCTTTTG TACCGACAAAATCCGTGCTC 20 20 45.0 50.0 57.7 58.3 0.0 0.0 0.568633 114194 115723 1648-1649 1.000000 75.9 ndhD_p4 ndhD 4 TCATTGTGCAACGAACCCTT TACCGACAAAATCCGTGCTC 20 20 45.0 50.0 58.3 58.3 0.0 0.0 0.596451 114194 115723 1651-1652 1.000000 75.7 ndhD_p5 ndhD 5 TTGTCATTGTGCAACGAACC TTACCGACAAAATCCGTGCT 20 20 45.0 45.0 57.5 57.8 0.0 0.0 0.656435 114194 115723 1655-1656 1.000000 75.2 ndhA_p1 ndhA 1 CCCTGACCAATGTATCTGGC CAATGTTACGAGCTTCCGGA 20 20 55.0 50.0 58.0 58.0 3.2 0.0 0.031710 118317 120449 2806-2809 1.000000 80.3 ndhA_p2 ndhA 2 TGACCAATGTATCTGGCAGC CAATGTTACGAGCTTCCGGA 20 20 50.0 50.0 58.0 58.0 10.3 0.0 0.047269 118317 120449 2803-2806 1.000000 80.2 ndhA_p3 ndhA 3 CCCTGACCAATGTATCTGGC TATTGGCGGAGAAGAAGCAC 20 20 55.0 50.0 58.0 58.0 3.2 0.0 0.051304 118317 120449 2847-2850 1.000000 80.2 ndhA_p4 ndhA 4 CAATGTATCTGGCAGCTCGT CAATGTTACGAGCTTCCGGA 20 20 50.0 50.0 58.1 58.0 10.3 0.0 0.059195 118317 120449 2799-2802 1.000000 80.1 ndhA_p5 ndhA 5 TGACCAATGTATCTGGCAGC TATTGGCGGAGAAGAAGCAC 20 20 50.0 50.0 58.0 58.0 10.3 0.0 0.066862 118317 120449 2844-2847 1.000000 80.0 ycf1_p1 ycf1 1 TCCAGGCCTCAATATCGACT TCAAGGAAATAAGAATCAAGGA 20 22 50.0 31.8 57.9 52.8 0.0 0.0 7.315791 123694 123813 203-203 1.000000 41.6 ycf1_p2 ycf1 2 TCCAGGCCTCAATATCGACT TCTTTCAAGGAAATAAGAATCAAGGA 20 26 50.0 30.8 57.9 56.8 0.0 0.0 7.256826 123694 123813 207-207 1.000000 41.5 ycf1_p3 ycf1 3 TCCAGGCCTCAATATCGACT CTCTTTCAAGGAAATAAGAATCAAGGA 20 27 50.0 33.3 57.9 57.8 0.0 0.0 7.324111 123694 123813 208-208 1.000000 41.5 ycf1_p4 ycf1 4 TCCAGGCCTCAATATCGACT TCTCTTTCAAGGAAATAAGAATCAAGG 20 27 50.0 33.3 57.9 57.8 0.0 0.0 7.324111 123694 123813 209-209 1.000000 41.5 ycf1_p5 ycf1 5 GTCCAGGCCTCAATATCGAC TCTTTCAAGGAAATAAGAATCAAGGA 20 26 55.0 30.8 57.8 56.8 0.0 0.0 7.325065 123694 123813 208-208 1.000000 41.5