primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	TAGGTGAATCCATGGAGGGG	20	20	50.0	55.0	58.0	58.2	0.0	21.3	0.210086	6709	7771	1158-1170	1.000000	81.7
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGG	20	20	45.0	55.0	58.0	58.2	1.4	21.3	0.218634	6709	7771	1124-1136	1.000000	81.7
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	TAGGTGAATCCATGGAGGGG	20	20	45.0	55.0	57.9	58.2	0.0	21.3	0.328693	6709	7771	1140-1152	1.000000	80.8
atpH-atpI_p4	atpH-atpI	4	GCGATACCTTCTACAGCCTG	TAGGTGAATCCATGGAGGGG	20	20	55.0	55.0	57.9	58.2	0.0	21.3	0.321783	6709	7771	1211-1223	1.000000	80.6
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	TAGGTGAATCCATGGAGGGG	20	20	45.0	55.0	58.2	58.2	1.4	21.3	0.364173	6709	7771	1125-1137	1.000000	80.6
trnM (CAU)-atpE_p1	trnM (CAU)-atpE	1	TATTGCTTTCATACGGCGGG	GTCGAGGCTATCAATGCGAT	20	20	50.0	50.0	58.4	57.9	13.2	0.0	0.483014	45669	45884	291-292	1.000000	82.7
trnM (CAU)-atpE_p2	trnM (CAU)-atpE	2	GTATTGCTTTCATACGGCGG	GTCGAGGCTATCAATGCGAT	20	20	50.0	50.0	57.3	57.9	13.2	0.0	0.734967	45669	45884	292-293	1.000000	80.7
trnM (CAU)-atpE_p3	trnM (CAU)-atpE	3	GCGGGAGTCATTGGTTCAAA	GTCGAGGCTATCAATGCGAT	20	20	50.0	50.0	58.8	57.9	0.0	0.0	0.827786	45669	45884	276-277	1.000000	80.3
trnM (CAU)-atpE_p4	trnM (CAU)-atpE	4	CTTTCATACGGCGGGAGTC	GTCGAGGCTATCAATGCGAT	19	20	57.9	50.0	58.0	57.9	13.2	0.0	1.098268	45669	45884	286-287	1.000000	77.9
trnM (CAU)-atpE_p5	trnM (CAU)-atpE	5	TATTGCTTTCATACGGCGGG	CTCTTCGACGAGCTAGGACA	20	20	50.0	55.0	58.4	58.6	13.2	5.9	1.040717	45669	45884	314-315	1.000000	77.8
atpB-rbcL_p1	atpB-rbcL	1	GGAAACCCCAGAACCAGAAG	CGTCTCTGTTTGTGGTGACA	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.154354	47780	48565	846-846	1.000000	80.8
atpB-rbcL_p2	atpB-rbcL	2	AGGACCGGACCAATGATTTG	CGTCTCTGTTTGTGGTGACA	20	20	50.0	50.0	57.9	58.1	25.0	0.0	0.194182	47780	48565	899-899	1.000000	80.4
atpB-rbcL_p3	atpB-rbcL	3	ACATCTAGGACCGGACCAAT	CGTCTCTGTTTGTGGTGACA	20	20	50.0	50.0	57.8	58.1	25.0	0.0	0.241659	47780	48565	905-905	1.000000	80.0
atpB-rbcL_p4	atpB-rbcL	4	GGAAACCCCAGAACCAGAAG	TCGTCTCTGTTTGTGGTGAC	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.307896	47780	48565	847-847	1.000000	79.6
atpB-rbcL_p5	atpB-rbcL	5	AGGACCGGACCAATGATTTG	TCGTCTCTGTTTGTGGTGAC	20	20	50.0	50.0	57.9	57.8	25.0	0.0	0.347724	47780	48565	900-900	1.000000	79.2
psbK-ORF100_p1	psbK-ORF100	1	CTCGTAGCAAGGGCAGAATT	AAGTCGCACGTTGCTTTCTA	20	20	50.0	45.0	58.0	58.1	0.0	8.1	0.169162	51526	52237	1318-1489	1.000000	87.6
psbK-ORF100_p2	psbK-ORF100	2	CTCGTAGCAAGGGCAGAATT	TAAGTCGCACGTTGCTTTCT	20	20	50.0	45.0	58.0	58.1	0.0	8.1	0.169162	51526	52237	1319-1490	1.000000	87.6
psbK-ORF100_p3	psbK-ORF100	3	CTCGTAGCAAGGGCAGAATT	GATTCGCGCCAAAATAGTGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.135161	51526	52237	962-962	0.500000	58.9
psbK-ORF100_p4	psbK-ORF100	4	CTTACAGCAGCTTGCCAAAC	GATTCGCGCCAAAATAGTGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.161417	51526	52237	1082-1082	0.500000	58.7
psbK-ORF100_p5	psbK-ORF100	5	CTCGTAGCAAGGGCAGAATT	CGATTCGCGTCAAAATCGTT	20	20	50.0	45.0	58.0	57.9	0.0	24.2	0.169175	51526	52237	910-910	0.500000	58.6
ORF100_p1	ORF100	1	CACGTGTCAAAGTCCTAGCA	GTTGAGCACCCCTTTCAAGA	20	20	50.0	50.0	57.9	58.0	7.6	0.0	0.167659	52238	52540	1178-1349	1.000000	88.0
ORF100_p2	ORF100	2	ACACGTGTCAAAGTCCTAGC	GTTGAGCACCCCTTTCAAGA	20	20	50.0	50.0	57.8	58.0	17.2	0.0	0.169141	52238	52540	1179-1350	1.000000	88.0
ORF100_p3	ORF100	3	ACGTGTCAAAGTCCTAGCAC	GTTGAGCACCCCTTTCAAGA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.169141	52238	52540	1177-1348	1.000000	88.0
ORF100_p4	ORF100	4	CACGTGTCAAAGTCCTAGCA	AAAACCCACTTTCCGAAGCT	20	20	50.0	45.0	57.9	57.9	7.6	0.0	0.216281	52238	52540	1276-1276	0.500000	58.3
ORF100_p5	ORF100	5	ACGTGTCAAAGTCCTAGCAC	AAAACCCACTTTCCGAAGCT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.217763	52238	52540	1275-1275	0.500000	58.3
trnK (UUU)-ORF148_p1	trnK (UUU)-ORF148	1	CTAGTTCCGGGTTCGAATCC	ACCGTGAGATTCAAATCATAGA	20	22	55.0	36.4	57.8	55.0	18.0	5.6	5.220175	56629	56807	235-236	1.000000	48.5
trnK (UUU)-ORF148_p2	trnK (UUU)-ORF148	2	CTAGTTCCGGGTTCGAATCC	ACCGTGAGATTCAAATCATAGAT	20	23	55.0	34.8	57.8	55.5	18.0	5.6	5.673048	56629	56807	235-236	1.000000	48.5
trnK (UUU)-ORF148_p3	trnK (UUU)-ORF148	3	CTAGTTCCGGGTTCGAATCC	ACCGTGAGATTCAAATCATAG	20	21	55.0	38.1	57.8	53.4	18.0	5.6	5.779095	56629	56807	235-236	1.000000	48.5
trnK (UUU)-ORF148_p4	trnK (UUU)-ORF148	4	ATCCGACTAGTTCCGGGTTC	ACCGTGAGATTCAAATCATAGA	20	22	55.0	36.4	58.9	55.0	19.6	5.6	5.890087	56629	56807	241-242	1.000000	48.3
trnK (UUU)-ORF148_p5	trnK (UUU)-ORF148	5	CTTCATCCGACTAGTTCCGG	ACCGTGAGATTCAAATCATAGA	20	22	55.0	36.4	57.5	55.0	15.1	5.6	5.493409	56629	56807	245-246	1.000000	48.1
clpP_p1	clpP	1	CGGTATAATCTTGGCTCGGG	TGACCCTGTTAGTCCGTTCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.085656	56999	57718	1353-1353	0.500000	59.3
clpP_p2	clpP	2	CGGTATAATCTTGGCTCGGG	AAGATCTGAACGAGTCGCAC	20	20	55.0	50.0	57.9	58.0	0.0	13.6	0.096314	56999	57718	1094-1094	0.500000	59.2
clpP_p3	clpP	3	CGGTATAATCTTGGCTCGGG	CAAGATCTGAACGAGTCGCA	20	20	55.0	50.0	57.9	58.0	0.0	13.6	0.097674	56999	57718	1095-1095	0.500000	59.2
clpP_p4	clpP	4	CGGTATAATCTTGGCTCGGG	CTGAACGAGTCGCACATACA	20	20	55.0	50.0	57.9	58.0	0.0	13.6	0.103098	56999	57718	1089-1089	0.500000	59.2
clpP_p5	clpP	5	CGGTATAATCTTGGCTCGGG	AGTTGTCCGCTTTCCTTTGT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.133068	56999	57718	1122-1122	0.500000	58.9
rps12-rpl20_p1	rps12-rpl20	1	GGAAGGCGTGATCCGTTTAT	AAACAGAAACACGACCGACA	20	20	50.0	45.0	58.1	58.0	5.7	0.0	0.063472	57904	58650	2100-2100	0.500000	59.5
rps12-rpl20_p2	rps12-rpl20	2	TCGATCTGGGGTCTGTACTC	AAACAGAAACACGACCGACA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.067169	57904	58650	2030-2030	0.500000	59.5
rps12-rpl20_p3	rps12-rpl20	3	GGAAGGCGTGATCCGTTTAT	AACAACAACCCGCAGATTCT	20	20	50.0	45.0	58.1	58.0	5.7	0.0	0.069278	57904	58650	2036-2036	0.500000	59.4
rps12-rpl20_p4	rps12-rpl20	4	TCGATCTGGGGTCTGTACTC	AACAACAACCCGCAGATTCT	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.072976	57904	58650	1966-1966	0.500000	59.4
rps12-rpl20_p5	rps12-rpl20	5	GAAGCTTCACGATCGCATTG	AAACAGAAACACGACCGACA	20	20	50.0	45.0	57.9	58.0	21.7	0.0	0.095670	57904	58650	1812-1812	0.500000	59.2
rpl20_p1	rpl20	1	AGAACGGACTAACAGGGTCA	AAACAGAAACACGACCGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.018307	58651	59061	1181-1181	0.500000	59.9
rpl20_p2	rpl20	2	AGAACGGACTAACAGGGTCA	AACAACAACCCGCAGATTCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.024114	58651	59061	1117-1117	0.500000	59.8
rpl20_p3	rpl20	3	GTGCGACTCGTTCAGATCTT	AAACAGAAACACGACCGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.028964	58651	59061	1440-1440	0.500000	59.8
rpl20_p4	rpl20	4	TGCGACTCGTTCAGATCTTG	AAACAGAAACACGACCGACA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.030325	58651	59061	1439-1439	0.500000	59.8
rpl20_p5	rpl20	5	GTGCGACTCGTTCAGATCTT	AACAACAACCCGCAGATTCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034771	58651	59061	1376-1376	0.500000	59.7
rps18_p1	rps18	1	GCGACGTAGAACAAAAAGGC	AGTTAGTGACGTGGGAAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.058849	59215	59679	1719-1743	1.000000	91.5
rps18_p2	rps18	2	GTCGTTTGTGGATCACTCGA	AGTTAGTGACGTGGGAAGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.065696	59215	59679	1594-1618	1.000000	91.5
rps18_p3	rps18	3	TCGTCGTTTGTGGATCACTC	AGTTAGTGACGTGGGAAGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.065696	59215	59679	1596-1620	1.000000	91.5
rps18_p4	rps18	4	GCGACGTAGAACAAAAAGGC	TAGGTTCATGGCCAAGGGTA	20	20	50.0	50.0	58.1	58.0	0.0	11.0	0.079451	59215	59679	1313-1370	1.000000	91.4
rps18_p5	rps18	5	ATTGACTCGGAAGGGAGACA	AGTTAGTGACGTGGGAAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.066786	59215	59679	1858-1858	0.500000	59.5
rpl33-psaJ_p1	rpl33-psaJ	1	ACACCCTTTCGAACACAAGT	ACATATCTTTCCGTGGCACC	20	20	45.0	50.0	57.6	58.0	0.0	12.9	0.451697	60046	60423	566-599	1.000000	80.0
rpl33-psaJ_p2	rpl33-psaJ	2	ACACCCTTTCGAACACAAGT	TAAGTGCTCTATGGTTCGCG	20	20	45.0	50.0	57.6	57.8	0.0	0.0	0.617110	60046	60423	541-574	1.000000	78.8
rpl33-psaJ_p3	rpl33-psaJ	3	ACACCCTTTCGAACACAAGT	GCTCTATGGTTCGCGTCTTT	20	20	45.0	50.0	57.6	58.4	0.0	5.8	0.771733	60046	60423	536-569	1.000000	77.6
rpl33-psaJ_p4	rpl33-psaJ	4	ACACCCTTTCGAACACAAGT	CGCGTCTTTAGCAGGTCTAT	20	20	45.0	50.0	57.6	57.5	0.0	0.0	0.907023	60046	60423	525-558	1.000000	76.6
rpl33-psaJ_p5	rpl33-psaJ	5	ACACCCTTTCGAACACAAGT	TCGCGTCTTTAGCAGGTCTA	20	20	45.0	50.0	57.6	58.5	0.0	5.8	0.957013	60046	60423	526-559	1.000000	76.2
rpl23-trnI (CAU)_p1	rpl23-trnI (CAU)	1	TCTTCTAGCCTTTCCCGGAA	CCTGTTAAGCATCCATGGCT	20	20	50.0	50.0	58.1	57.9	0.7	7.0	0.115986	82998	83516	914-1061	1.000000	86.4
rpl23-trnI (CAU)_p2	rpl23-trnI (CAU)	2	CGGTATAGACGCTTATGGCC	CCTGTTAAGCATCCATGGCT	20	20	55.0	50.0	58.0	57.9	0.0	7.0	0.070801	82998	83516	1198-1340	1.000000	85.5
rpl23-trnI (CAU)_p3	rpl23-trnI (CAU)	3	ACTGTCTACGGTTCCATTGC	CCTGTTAAGCATCCATGGCT	20	20	50.0	50.0	57.9	57.9	0.0	7.0	0.157733	82998	83516	1067-1209	1.000000	85.5
rpl23-trnI (CAU)_p4	rpl23-trnI (CAU)	4	TTATGGCCTCCCCCTCTATG	CCTGTTAAGCATCCATGGCT	20	20	55.0	50.0	58.0	57.9	3.3	7.0	0.107763	82998	83516	1186-1328	1.000000	85.3
rpl23-trnI (CAU)_p5	rpl23-trnI (CAU)	5	ACCACAAAGATGCTGTCCAG	CCTGTTAAGCATCCATGGCT	20	20	50.0	50.0	58.1	57.9	0.0	7.0	0.161380	82998	83516	1130-1130	0.500000	58.7
ndhD-ccsA_p1	ndhD-ccsA	1	CCGGATTTTGTTCTTTCACTCTC	AGGGCTACATAGTTATGGTTCA	23	22	43.5	40.9	58.0	56.3	0.0	4.3	6.710998	122521	122790	367-376	1.000000	45.4
ndhD-ccsA_p2	ndhD-ccsA	2	TCCGGATTTTGTTCTTTCACTC	AGGGCTACATAGTTATGGTTCA	22	22	40.9	40.9	56.9	56.3	0.0	4.3	6.767309	122521	122790	368-377	1.000000	45.4
ndhD-ccsA_p3	ndhD-ccsA	3	CCGGATTTTGTTCTTTCACTCT	AGGGCTACATAGTTATGGTTCA	22	22	40.9	40.9	56.9	56.3	0.0	4.3	6.774995	122521	122790	367-376	1.000000	45.4
ndhD-ccsA_p4	ndhD-ccsA	4	TCCGGATTTTGTTCTTTCACT	AGGGCTACATAGTTATGGTTCA	21	22	38.1	40.9	55.7	56.3	0.0	4.3	6.959364	122521	122790	368-377	1.000000	45.4
ndhD-ccsA_p5	ndhD-ccsA	5	TCCGGATTTTGTTCTTTCACTCT	AGGGCTACATAGTTATGGTTCA	23	22	39.1	40.9	58.3	56.3	0.0	4.3	6.960582	122521	122790	368-377	1.000000	45.4
ycf1_p1	ycf1	1	GGATCCTTTTCGTCCTGCTT	AAATGCAGTCAGCCATAGGG	20	20	50.0	50.0	57.9	57.9	3.8	0.0	0.178313	136364	136483	1557-1557	0.500000	58.6
ycf1_p2	ycf1	2	GGATCCTTTTCGTCCTGCTT	TTACCGCTCACAGTTAAGGC	20	20	50.0	50.0	57.9	57.9	3.8	0.0	0.204292	136364	136483	950-950	0.500000	58.4
ycf1_p3	ycf1	3	TGGATTAGTGTAGTCGGGCA	AAATGCAGTCAGCCATAGGG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.207675	136364	136483	1179-1179	0.500000	58.3
ycf1_p4	ycf1	4	GGATCCTTTTCGTCCTGCTT	ATCGAACGTAAGCCGAAGAC	20	20	50.0	50.0	57.9	58.1	3.8	0.0	0.209893	136364	136483	911-911	0.500000	58.3
ycf1_p5	ycf1	5	TGGATTAGTGTAGTCGGGCA	TTACCGCTCACAGTTAAGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.233654	136364	136483	572-572	0.500000	58.1
trnI (CAU)-rpl23_p1	trnI (CAU)-rpl23	1	CCTGTTAAGCATCCATGGCT	TCTTCTAGCCTTTCCCGGAA	20	20	50.0	50.0	57.9	58.1	7.0	0.7	0.115986	164899	165417	914-1061	1.000000	86.4
trnI (CAU)-rpl23_p2	trnI (CAU)-rpl23	2	CCTGTTAAGCATCCATGGCT	CGGTATAGACGCTTATGGCC	20	20	50.0	55.0	57.9	58.0	7.0	0.0	0.070801	164899	165417	1198-1340	1.000000	85.5
trnI (CAU)-rpl23_p3	trnI (CAU)-rpl23	3	CCTGTTAAGCATCCATGGCT	ACTGTCTACGGTTCCATTGC	20	20	50.0	50.0	57.9	57.9	7.0	0.0	0.157733	164899	165417	1067-1209	1.000000	85.5
trnI (CAU)-rpl23_p4	trnI (CAU)-rpl23	4	CCTGTTAAGCATCCATGGCT	TTATGGCCTCCCCCTCTATG	20	20	50.0	55.0	57.9	58.0	7.0	3.3	0.107763	164899	165417	1186-1328	1.000000	85.3
trnI (CAU)-rpl23_p5	trnI (CAU)-rpl23	5	CCTGTTAAGCATCCATGGCT	ACCACAAAGATGCTGTCCAG	20	20	50.0	50.0	57.9	58.1	7.0	0.0	0.161380	164899	165417	1130-1130	0.500000	58.7