primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.107227	102	362	438-440	1.000000	80.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.140446	102	362	388-390	1.000000	80.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.149276	102	362	391-393	1.000000	80.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	102	362	377-379	1.000000	80.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	102	362	391-393	1.000000	79.9
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4253	5267	1047-1087	1.000000	82.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.2	58.0	20.3	0.0	0.186962	4253	5267	1046-1086	1.000000	82.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	CGGAACTTCGCCCTAATCAA	20	20	50.0	50.0	58.3	58.0	5.3	0.0	0.301348	4253	5267	1056-1096	1.000000	81.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4253	5267	1102-1142	1.000000	81.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4253	5267	1101-1141	1.000000	81.2
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAATAGG	20	24	50.0	37.5	58.0	57.9	0.0	0.0	4.172656	9887	10122	317-363	1.000000	52.7
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	TGGAAATAAATTGCGTCCAATAGG	20	24	55.0	37.5	58.1	57.9	0.0	0.0	4.201493	9887	10122	320-366	1.000000	52.4
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAA	20	20	50.0	35.0	58.0	53.8	0.0	0.0	4.269330	9887	10122	317-363	1.000000	51.9
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	TGGAAATAAATTGCGTCCAA	20	20	55.0	35.0	58.1	53.8	0.0	0.0	4.298167	9887	10122	320-366	1.000000	51.7
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.404255	9887	10122	276-322	1.000000	51.1
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	21	20	42.9	50.0	57.4	58.4	5.3	0.0	1.948668	10195	11202	917-1205	1.000000	72.8
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	21	20	42.9	50.0	57.4	58.4	5.3	0.0	1.950350	10195	11202	916-1204	1.000000	72.8
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	21	20	47.6	50.0	57.3	58.4	0.0	0.0	2.067175	10195	11202	856-1144	1.000000	72.1
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	20	20	40.0	50.0	55.9	58.4	0.0	0.0	2.453105	10195	11202	917-1205	1.000000	68.8
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	TTGGAATGAAAAGCGTCCAT	AGACATTTACCGACGAAGCG	20	20	40.0	50.0	55.9	58.4	0.0	0.0	2.453105	10195	11202	918-1206	1.000000	68.8
atpH-atpI_p1	atpH-atpI	1	AATAGAAGCAAGCCCGACAG	GCGAATCCATGGAAGGTCAT	20	20	50.0	50.0	58.0	58.0	0.0	20.7	0.056966	14766	15956	1278-1283	1.000000	80.0
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	1.4	20.7	0.065515	14766	15956	1244-1249	1.000000	80.0
atpH-atpI_p3	atpH-atpI	3	AATAGAAGCAAGCCCGACAG	TTTTTGCAACCTTAGCTGCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	14766	15956	1308-1313	1.000000	79.2
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACCTTAGCTGCG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	14766	15956	1274-1279	1.000000	79.1
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.2	58.0	1.4	20.7	0.211053	14766	15956	1245-1250	1.000000	78.8
petN-psbM_p1	petN-psbM	1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	20	20	50.0	45.0	58.0	57.6	0.0	0.0	0.465211	30560	31723	1270-1325	1.000000	76.5
petN-psbM_p2	petN-psbM	2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.554432	30560	31723	1213-1268	1.000000	75.8
petN-psbM_p3	petN-psbM	3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	58.1	57.6	0.0	0.0	0.554432	30560	31723	1212-1267	1.000000	75.8
petN-psbM_p4	petN-psbM	4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	20	20	55.0	45.0	57.8	57.6	0.0	0.0	0.611620	30560	31723	1211-1266	1.000000	75.4
petN-psbM_p5	petN-psbM	5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	20	20	60.0	45.0	58.2	57.6	6.7	0.0	0.652301	30560	31723	1208-1263	1.000000	75.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGCCAGACCAACCTACAAAA	20	20	55.0	50.0	58.1	58.0	7.6	0.0	0.074361	34394	35846	1592-1649	1.000000	81.6
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGCCAGACCAACCTACAAAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096516	34394	35846	1595-1652	1.000000	81.4
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TAGGCCAGACCAACCTACAA	20	20	55.0	50.0	58.1	58.0	7.6	5.0	0.097032	34394	35846	1594-1651	1.000000	81.4
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	20	20	55.0	45.0	58.1	58.0	7.6	0.0	0.106399	34394	35846	1582-1639	1.000000	81.4
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	TAGGCCAGACCAACCTACAA	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.119187	34394	35846	1597-1654	1.000000	81.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.7	57.8	0.0	1.1	0.446564	39128	39574	587-605	1.000000	80.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	GGGTTGGTCGGGTAACAAAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.3	57.8	0.0	1.1	0.479849	39128	39574	573-591	1.000000	80.4
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTCCGGCACATCATTATGGA	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	57.0	57.8	0.0	1.1	1.188961	39128	39574	543-561	1.000000	75.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	AGGGTTGGTCGGGTAACAAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	59.2	57.8	0.0	1.1	1.324496	39128	39574	574-592	1.000000	73.6
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	ATTTGCTTCTCCTGAGGGTTG	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	58.2	57.8	0.0	1.1	1.369583	39128	39574	588-606	1.000000	73.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49684	50887	1232-1254	1.000000	81.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49684	50887	1240-1262	1.000000	81.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	49684	50887	1264-1286	1.000000	80.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	49684	50887	1272-1294	1.000000	80.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	49684	50887	1246-1268	1.000000	79.6
petA-psbJ_p1	petA-psbJ	1	TGCTCTTCTTGGCATCTGTT	AGCCGGTATTCTTGTGATCG	20	20	45.0	50.0	57.4	57.8	0.0	1.9	0.799074	66632	67611	1150-1152	1.000000	73.8
petA-psbJ_p2	petA-psbJ	2	TTGCTCTTCTTGGCATCTGT	AGCCGGTATTCTTGTGATCG	20	20	45.0	50.0	57.4	57.8	0.0	1.9	0.799074	66632	67611	1151-1153	1.000000	73.8
petA-psbJ_p3	petA-psbJ	3	CGTGTCCAAGGTCTTTTGCT	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	58.7	57.8	0.0	1.9	0.916070	66632	67611	1166-1168	1.000000	72.9
petA-psbJ_p4	petA-psbJ	4	GGTCTTTTGCTCTTCTTGGC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.3	57.8	0.0	1.9	0.938964	66632	67611	1157-1159	1.000000	72.7
petA-psbJ_p5	petA-psbJ	5	TCCATTACGTGTCCAAGGTC	AGCCGGTATTCTTGTGATCG	20	20	50.0	50.0	57.2	57.8	0.0	1.9	0.980015	66632	67611	1173-1175	1.000000	72.4
petL-petG_p1	petL-petG	1	CGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	20	20	55.0	50.0	58.2	57.8	7.7	0.0	0.326022	69717	69874	291-291	1.000000	77.4
petL-petG_p2	petL-petG	2	TCGGTCTGAGCAAGATACGA	CAACTGATCACCACGTCTGT	20	20	50.0	50.0	58.3	57.8	7.7	0.0	0.408384	69717	69874	292-292	1.000000	76.7
petL-petG_p3	petL-petG	3	CGGTCTGAGCAAGATACGAC	ATCCAACTGATCACCACGTC	20	20	55.0	50.0	58.2	57.6	7.7	0.0	0.557829	69717	69874	294-294	1.000000	75.5
petL-petG_p4	petL-petG	4	TCGGTCTGAGCAAGATACGA	ATCCAACTGATCACCACGTC	20	20	50.0	50.0	58.3	57.6	7.7	0.0	0.640191	69717	69874	295-295	1.000000	74.9
petL-petG_p5	petL-petG	5	CGGTCTGAGCAAGATACGAC	CCAACTGATCACCACGTCTG	20	20	55.0	55.0	58.2	58.6	7.7	0.0	0.738654	69717	69874	292-292	1.000000	74.1
petG-trnW-CCA_p1	petG-trnW-CCA	1	ACAGACGTGGTGATCAGTTG	TTCAAATCCTACAGGGCGTG	20	20	50.0	50.0	57.8	57.9	0.0	5.5	0.262804	69989	70112	173-190	1.000000	87.7
petG-trnW-CCA_p2	petG-trnW-CCA	2	ACAGACGTGGTGATCAGTTG	GTTCAAATCCTACAGGGCGT	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.264278	69989	70112	174-191	1.000000	87.7
petG-trnW-CCA_p3	petG-trnW-CCA	3	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.191682	69989	70112	206-223	1.000000	87.4
petG-trnW-CCA_p4	petG-trnW-CCA	4	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.243803	69989	70112	192-209	1.000000	87.3
petG-trnW-CCA_p5	petG-trnW-CCA	5	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.8	58.0	0.0	2.6	0.170881	69989	70112	228-245	1.000000	86.8
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72771	73541	927-951	1.000000	75.1
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	72771	73541	926-950	1.000000	75.1
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.844694	72771	73541	890-914	1.000000	73.6
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.844694	72771	73541	889-913	1.000000	73.6
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	AGCGACGAGGAACATGTACT	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.939077	72771	73541	888-912	1.000000	72.8
rps11-rpl36_p1	rps11-rpl36	1	ACGTCTATTTCTACGCGAACC	AAGAGCCTCCGTTCGTAAAA	21	20	47.6	45.0	58.2	57.2	15.9	0.0	2.035474	83357	83522	317-327	1.000000	64.7
rps11-rpl36_p2	rps11-rpl36	2	CGTCTATTTCTACGCGAACCA	AAGAGCCTCCGTTCGTAAAA	21	20	47.6	45.0	58.2	57.2	15.9	0.0	2.036653	83357	83522	316-326	1.000000	64.7
rps11-rpl36_p3	rps11-rpl36	3	ACGTCTATTTCTACGCGAACC	AAATAAGAGCCTCCGTTCGT	21	20	47.6	45.0	58.2	56.9	15.9	0.0	2.262613	83357	83522	321-331	1.000000	62.9
rps11-rpl36_p4	rps11-rpl36	4	ACGTCTATTTCTACGCGAACC	AGAGCCTCCGTTCGTAAAAT	21	20	47.6	45.0	58.2	56.9	15.9	0.0	2.262613	83357	83522	316-326	1.000000	62.9
rps11-rpl36_p5	rps11-rpl36	5	CGTCTATTTCTACGCGAACCA	AGAGCCTCCGTTCGTAAAAT	21	20	47.6	45.0	58.2	56.9	15.9	0.0	2.263792	83357	83522	315-325	1.000000	62.9
ndhF-rpl32_p1	ndhF-rpl32	1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	20	20	40.0	45.0	57.0	57.8	0.0	13.1	1.214860	116825	117943	1300-1315	1.000000	72.5
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	20	20	40.0	45.0	57.0	57.8	0.0	0.0	1.261149	116825	117943	1298-1313	1.000000	72.1
ndhF-rpl32_p3	ndhF-rpl32	3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	20	20	40.0	55.0	57.0	58.3	0.0	0.0	1.377810	116825	117943	1287-1302	1.000000	71.2
ndhF-rpl32_p4	ndhF-rpl32	4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	20	20	40.0	45.0	57.0	57.6	0.0	0.0	1.489633	116825	117943	1297-1312	1.000000	70.3
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	21	20	38.1	45.0	57.7	57.8	0.0	13.1	1.495037	116825	117943	1301-1316	1.000000	70.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.182194	118103	119583	1591-1674	1.000000	82.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	14.0	0.0	0.183564	118103	119583	1592-1675	1.000000	82.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.228483	118103	119583	1593-1676	1.000000	82.5
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.229854	118103	119583	1594-1677	1.000000	82.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.8	57.9	14.0	0.0	0.298641	118103	119583	1601-1684	1.000000	81.9
ndhG-ndhI_p1	ndhG-ndhI	1	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTT	20	20	50.0	40.0	58.0	56.4	0.0	0.0	1.576114	124184	124633	531-566	1.000000	71.5
ndhG-ndhI_p2	ndhG-ndhI	2	AATACCACCCCCATACCTCC	TCAAATTGCTTTGGGTCGTT	20	20	55.0	40.0	58.2	56.4	0.0	0.0	1.728233	124184	124633	586-621	1.000000	70.3
ndhG-ndhI_p3	ndhG-ndhI	3	TGTATTGGTCCGGGCAAATC	TCAAATTGCTTTGGGTCGTT	20	20	50.0	40.0	58.2	56.4	13.2	0.0	1.799688	124184	124633	526-561	1.000000	69.8
ndhG-ndhI_p4	ndhG-ndhI	4	AATCATGTATTGGTCCGGGC	ATCAAATTGCTTTGGGTCGT	20	20	50.0	40.0	58.0	56.2	0.0	0.0	1.807085	124184	124633	532-567	1.000000	69.7
ndhG-ndhI_p5	ndhG-ndhI	5	AATCATGTATTGGTCCGGGC	TCAAATTGCTTTGGGTCGTTT	20	21	50.0	38.1	58.0	57.2	0.0	0.0	1.829054	124184	124633	531-566	1.000000	69.5