primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GAGAAGCCTTTCCCGCATTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.062727	4338	4904	1052-1215	0.833333	80.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AGAAGCCTTTCCCGCATTAG	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.0	58.0	0.0	7.6	0.068463	4338	4904	1051-1214	0.833333	80.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	GAGAAGCCTTTCCCGCATTA	ACGAGACAAGAAGGGGGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.058194	4338	4904	1476-1641	0.833333	79.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGAAGCCTTTCCCGCATTAG	ACGAGACAAGAAGGGGGTTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.063930	4338	4904	1475-1640	0.833333	79.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGATCCGGTAAGAATGCAA	ACGAGACAAGAAGGGGGTTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071703	4338	4904	1280-1445	0.666667	67.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6008	6970	750-1056	1.000000	90.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6008	6970	761-1067	1.000000	89.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6008	6970	754-1060	1.000000	85.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6008	6970	780-1086	1.000000	85.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	6008	6970	762-1068	1.000000	85.2
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TCTATGGAGGGTCATCATTGA	20	21	45.0	42.9	58.0	55.5	1.4	0.0	3.497320	13305	14247	869-1061	1.000000	64.0
atpH-atpI_p2	atpH-atpI	2	AGCGGCAGAAATCAATGGAT	TCTATGGAGGGTCATCATTGA	20	21	45.0	42.9	57.9	55.5	1.4	0.0	3.521904	13305	14247	861-1053	1.000000	63.8
atpH-atpI_p3	atpH-atpI	3	AAGCGGCAGAAATCAATGGA	TCTATGGAGGGTCATCATTGA	20	21	45.0	42.9	58.2	55.5	1.4	0.0	3.620821	13305	14247	862-1054	1.000000	63.0
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	TCTATGGAGGGTCATCATTGA	20	21	45.0	42.9	58.2	55.5	1.4	0.0	3.642859	13305	14247	870-1062	1.000000	62.9
atpH-atpI_p5	atpH-atpI	5	GAAGCGGCAGAAATCAATGG	TCTATGGAGGGTCATCATTGA	20	21	50.0	42.9	57.8	55.5	1.4	0.0	3.658225	13305	14247	863-1055	1.000000	62.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.746530	26444	27636	1214-1281	1.000000	86.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	ATTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.3	58.4	0.0	14.6	1.019893	26444	27636	1196-1263	1.000000	83.8
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	21	20	47.6	50.0	57.7	58.1	0.0	0.0	1.401159	26444	27636	1216-1283	1.000000	80.8
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	ATTCCCTCATTTCCATCCCG	GACACCCGGATTTGAACTGG	20	20	50.0	55.0	57.3	58.8	0.0	0.7	1.499132	26444	27636	1219-1286	1.000000	80.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCCCTCATTTCCATCCCGG	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	59.4	58.1	0.0	0.0	1.531214	26444	27636	1213-1280	1.000000	79.8
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AAATGCAAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	23	20	26.1	50.0	52.7	58.5	0.0	5.4	8.816568	29185	29629	506-941	1.000000	52.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	18	20	44.4	50.0	52.6	58.5	0.0	5.4	7.894962	29185	29629	527-962	1.000000	51.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	18	20	44.4	50.0	52.6	57.0	0.0	5.4	8.415115	29185	29629	528-963	1.000000	51.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	18	19	44.4	52.6	52.6	57.8	0.0	5.4	8.649364	29185	29629	526-961	1.000000	51.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	18	21	44.4	47.6	52.6	58.5	0.0	5.4	8.909104	29185	29629	529-964	1.000000	51.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30297	30885	532-733	1.000000	90.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	ACCCGCTGTTCCAATTGAAT	20	20	55.0	45.0	57.8	58.1	0.0	7.1	0.235823	30297	30885	855-1060	1.000000	89.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	AAAAGACCCGCTGTTCCAAT	20	20	55.0	45.0	57.8	58.0	0.0	0.0	0.163803	30297	30885	860-1065	0.833333	80.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TTTCGTAGTACCCTACCCCC	AAAGACCCGCTGTTCCAATT	20	20	55.0	45.0	57.8	58.0	0.0	0.0	0.163803	30297	30885	859-1064	0.833333	80.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CCAGCGTAGACATATTGCCA	AAAGACCCGCTGTTCCAATT	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.243566	30297	30885	974-1179	0.833333	79.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GTTGGACGTTCCTACAGCAT	GCCTTCAGCCTTTCGATGTA	20	20	50.0	50.0	57.9	58.0	11.5	0.0	0.118938	45335	46283	1276-1416	0.833333	83.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GTTGGACGTTCCTACAGCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.9	58.0	11.5	0.0	0.099386	45335	46283	1054-1235	0.833333	81.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TTGGAAAAGGGGAGAAAGCC	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014305	45335	46283	1018-1136	0.666667	71.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	TTGGAAAAGGGGAGAAAGCC	GCCTTCAGCCTTTCGATGTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033857	45335	46283	1240-1359	0.666667	71.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TTGGAAAAGGGGAGAAAGCC	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.079114	45335	46283	1026-1144	0.666667	71.0
petA-psbJ_p1	petA-psbJ	1	CAGGGCCAGAACTTCTTGTT	ATGTGGAGGGAAGTGGGTTA	20	20	50.0	50.0	58.0	57.9	6.2	0.0	0.066864	61954	62684	944-1109	1.000000	91.5
petA-psbJ_p2	petA-psbJ	2	CAGGGCCAGAACTTCTTGTT	ACGGACTAGCTGTACCTACC	20	20	50.0	55.0	58.0	58.0	6.2	0.0	0.063006	61954	62684	1290-1455	1.000000	90.5
petA-psbJ_p3	petA-psbJ	3	CAGGGCCAGAACTTCTTGTT	GGACTAGCTGTACCTACCGT	20	20	50.0	55.0	58.0	58.0	6.2	0.0	0.063006	61954	62684	1288-1453	1.000000	90.5
petA-psbJ_p4	petA-psbJ	4	CAGGGCCAGAACTTCTTGTT	GAGCCGACAAGGAATTCCAT	20	20	50.0	50.0	58.0	58.0	6.2	26.2	0.057240	61954	62684	1430-1595	1.000000	90.0
petA-psbJ_p5	petA-psbJ	5	CAGGGCCAGAACTTCTTGTT	TCCCTATTCATTGCGGGTTG	20	20	50.0	50.0	58.0	57.9	6.2	0.0	0.064452	61954	62684	1519-1684	1.000000	89.6
petG-trnW-CCA_p1	petG-trnW-CCA	1	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.8	58.0	0.0	2.6	0.170881	65166	65307	236-246	1.000000	90.6
petG-trnW-CCA_p2	petG-trnW-CCA	2	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.191682	65166	65307	214-224	1.000000	90.5
petG-trnW-CCA_p3	petG-trnW-CCA	3	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.8	57.9	0.0	1.4	0.243803	65166	65307	200-210	1.000000	90.0
petG-trnW-CCA_p4	petG-trnW-CCA	4	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.504151	65166	65307	234-244	1.000000	88.0
petG-trnW-CCA_p5	petG-trnW-CCA	5	ACAGACGTGGTGATCAGTTG	CCCGATGTCGTAGGTTCAAA	20	20	50.0	50.0	57.8	57.6	0.0	1.4	0.528394	65166	65307	195-205	1.000000	87.8
ndhF_p1	ndhF	1	AAAACCTCTTGTGACCCGTC	TTCATGGACTTCGTTCGGAA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.860752	108290	110527	2500-2591	0.833333	74.1
ndhF_p2	ndhF	2	AAAACCTCTTGTGACCCGTC	TTTCATGGACTTCGTTCGGA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.860752	108290	110527	2501-2592	0.833333	74.1
ndhF_p3	ndhF	3	GCAGAATACCGTCACCCATT	TTCATGGACTTCGTTCGGAA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.862311	108290	110527	2891-2982	0.833333	73.7
ndhF_p4	ndhF	4	GCAGAATACCGTCACCCATT	TTTCATGGACTTCGTTCGGA	20	20	50.0	45.0	58.0	57.2	0.0	0.0	0.862311	108290	110527	2892-2983	0.833333	73.7
ndhF_p5	ndhF	5	CTTGTGACCCGTCTTTTCGA	TTCATGGACTTCGTTCGGAA	20	20	50.0	45.0	58.1	57.2	0.0	0.0	0.963248	108290	110527	2493-2584	0.833333	73.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTGGGCAGCGTTAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032171	111761	112235	577-675	1.000000	91.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGATATTGGGCAGCGTTAA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033541	111761	112235	578-676	1.000000	91.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGATATTGGGCAGCGTTAA	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.113283	111761	112235	1069-1249	1.000000	91.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TTGACCCATTGAGCTTGACC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.098131	111761	112235	1137-1137	0.166667	45.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTGACCCATTGAGCTTGACC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.099501	111761	112235	1138-1138	0.166667	45.9
ccsA-ndhD_p1	ccsA-ndhD	1	ACCCAAGGCTTTGCTACTTC	TACCCCGCGCTCATTCTATA	20	20	50.0	50.0	58.1	58.1	2.4	5.6	0.175165	113382	113572	1516-1631	1.000000	86.4
ccsA-ndhD_p2	ccsA-ndhD	2	CAAATTCCGCAATTGTGGCT	ATCGATGGCTTCACTTGCAT	20	20	45.0	45.0	57.9	58.0	29.6	0.0	0.131611	113382	113572	656-771	0.833333	77.2
ccsA-ndhD_p3	ccsA-ndhD	3	CAAATTCCGCAATTGTGGCT	TTCAACATCTCCTGGCCAAC	20	20	45.0	50.0	57.9	58.1	29.6	7.0	0.173467	113382	113572	923-1038	0.833333	76.5
ccsA-ndhD_p4	ccsA-ndhD	4	CAAATTCCGCAATTGTGGCT	TACCCCGCGCTCATTCTATA	20	20	45.0	50.0	57.9	58.1	29.6	5.6	0.169389	113382	113572	993-1108	0.833333	76.4
ccsA-ndhD_p5	ccsA-ndhD	5	ACCCAAGGCTTTGCTACTTC	ATCGATGGCTTCACTTGCAT	20	20	50.0	45.0	58.1	58.0	2.4	0.0	0.137387	113382	113572	1179-1294	0.833333	76.3
ycf1_p1	ycf1	1	GTTCGACGATAAGGCCCATT	AAAACCTCTTGTGACCCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087363	125900	126049	444-450	1.000000	87.8
ycf1_p2	ycf1	2	GAACGAATCTGATCCCCCAC	AAAACCTCTTGTGACCCGTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.081901	125900	126049	915-924	0.666667	66.4
ycf1_p3	ycf1	3	GTGAACGAATCTGATCCCCC	AAAACCTCTTGTGACCCGTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.081901	125900	126049	917-926	0.666667	66.4
ycf1_p4	ycf1	4	GAACGAATCTGATCCCCCAC	GCAGAATACCGTCACCCATT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.083460	125900	126049	1306-1315	0.666667	66.4
ycf1_p5	ycf1	5	GTGAACGAATCTGATCCCCC	GCAGAATACCGTCACCCATT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.083460	125900	126049	1308-1317	0.666667	66.4