primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGAATCCCTTGCTTCATCCA	GCTCAACCTACAGAAACCGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.208005	4299	4905	752-789	1.000000	79.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	ACGAATCCCTTGCTTCATCC	GCTCAACCTACAGAAACCGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.209441	4299	4905	753-790	1.000000	79.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	GCTCAACCTACAGAAACCGT	20	20	50.0	50.0	58.2	57.8	20.3	0.0	0.333738	4299	4905	704-741	1.000000	78.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	GCTCAACCTACAGAAACCGT	20	20	50.0	50.0	58.2	57.8	20.3	0.0	0.333738	4299	4905	703-740	1.000000	78.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGAATCCCTTGCTTCATCCA	AAGAGGGCGGGAATTTTGAA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.345273	4299	4905	719-756	1.000000	78.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CCACAACGGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	11.1	16.9	0.189961	6056	6852	884-891	1.000000	81.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGTCGAGTTAAGAGCATCCC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.2	58.1	0.0	16.9	0.234651	6056	6852	931-938	1.000000	81.0
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	6056	6852	858-865	1.000000	81.0
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6056	6852	874-881	1.000000	80.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CCACAACGGATCATGTCCTT	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	57.9	58.2	11.1	0.0	0.284881	6056	6852	914-921	1.000000	80.7
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	TTTGCAACTTTAGCTGCAGC	20	20	45.0	45.0	58.1	57.9	1.4	31.5	0.242376	13638	14318	763-765	1.000000	79.1
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	TTTGCAACTTTAGCTGCAGC	20	20	50.0	45.0	57.9	57.9	0.0	31.5	0.250875	13638	14318	821-823	1.000000	79.0
atpH-atpI_p3	atpH-atpI	3	AACAGAAGCGGCAGAAATCA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.1	57.8	1.4	21.3	0.279658	13638	14318	734-736	1.000000	78.9
atpH-atpI_p4	atpH-atpI	4	AGCCAATCCAGCAGCAATAA	TTTGCAACTTTAGCTGCAGC	20	20	45.0	45.0	57.9	57.9	0.0	31.5	0.287042	13638	14318	781-783	1.000000	78.7
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.9	57.8	0.0	21.3	0.288157	13638	14318	792-794	1.000000	78.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	31480	32035	659-660	1.000000	78.8
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31480	32035	638-639	1.000000	78.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	58.3	58.2	0.0	0.0	0.414292	31480	32035	656-657	1.000000	77.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.432442	31480	32035	635-636	1.000000	77.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	20	20	55.0	50.0	58.3	58.3	0.0	0.0	0.546195	31480	32035	661-662	1.000000	76.6
psaA-ycf3_p1	psaA-ycf3	1	GGGGCCCTTAGCTATTGTTC	ATCAAGCCGCTGAGTATTGG	20	20	55.0	50.0	58.0	58.0	24.4	0.0	0.058298	42477	43296	1028-1038	1.000000	82.5
psaA-ycf3_p2	psaA-ycf3	2	GGGGCCCTTAGCTATTGTTC	ATTGGTTGAAGATCACGGGG	20	20	55.0	50.0	58.0	57.9	24.4	0.0	0.146110	42477	43296	962-972	1.000000	82.0
psaA-ycf3_p3	psaA-ycf3	3	GGGGCCCTTAGCTATTGTTC	CGATCAAGCCGCTGAGTATT	20	20	55.0	50.0	58.0	58.1	24.4	0.0	0.159248	42477	43296	1030-1040	1.000000	81.7
psaA-ycf3_p4	psaA-ycf3	4	AAAAATGACCGGGTCTAGCC	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	26.4	0.0	0.165236	42477	43296	1005-1015	1.000000	81.7
psaA-ycf3_p5	psaA-ycf3	5	AAAAATGACCGGGTCTAGCC	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	26.4	0.0	0.253048	42477	43296	939-949	1.000000	81.2
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	57.5	58.0	0.0	17.4	0.476915	48950	49284	389-390	1.000000	76.8
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.5	58.1	0.0	20.1	0.526305	48950	49284	425-426	1.000000	76.4
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.564710	48950	49284	440-441	1.000000	76.0
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.610708	48950	49284	401-402	1.000000	75.7
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	20	20	50.0	50.0	57.5	58.4	0.0	0.0	0.854756	48950	49284	441-442	1.000000	73.7
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	CCCGAAAGTTGGGTAGGATG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.040892	49358	49993	784-815	1.000000	82.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	CCGAAAGTTGGGTAGGATGG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.040892	49358	49993	783-814	1.000000	82.5
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGGTAGGATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	49358	49993	748-779	1.000000	82.2
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGGTAGGATGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.090283	49358	49993	747-778	1.000000	82.2
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	AAAGGGGAATGGTCGGGATA	CCGAAAGTTGGGTAGGATGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.136739	49358	49993	797-828	1.000000	81.7
rps18-rpl20_p1	rps18-rpl20	1	CGAGTCGACTACTAGAACTGC	GAAAGAATCCCCCGAAACGA	21	20	52.4	50.0	57.7	57.9	20.1	0.0	1.410876	69561	69842	321-359	1.000000	73.9
rps18-rpl20_p2	rps18-rpl20	2	CGAGTCGACTACTAGAACTGC	AAAGAATCCCCCGAAACGAT	21	20	52.4	45.0	57.7	57.2	20.1	0.0	2.108416	69561	69842	320-358	1.000000	68.3
rps18-rpl20_p3	rps18-rpl20	3	CGAGTCGACTACTAGAACTGC	AAGAATCCCCCGAAACGATT	21	20	52.4	45.0	57.7	57.2	20.1	0.0	2.108416	69561	69842	319-357	1.000000	68.3
rps18-rpl20_p4	rps18-rpl20	4	CGAGTCGACTACTAGAACTGC	AGAATCCCCCGAAACGATTT	21	20	52.4	45.0	57.7	57.2	20.1	0.0	2.108416	69561	69842	318-356	1.000000	68.3
rps18-rpl20_p5	rps18-rpl20	5	CGAGTCGACTACTAGAACTGC	GGAAAGAATCCCCCGAAACG	21	20	52.4	55.0	57.7	58.9	20.1	0.0	2.214991	69561	69842	322-360	1.000000	67.5
rpl20-rps12_p1	rpl20-rps12	1	CCCTCGAAAGCTTGATCCAA	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.234977	70230	71007	879-883	1.000000	79.5
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.234977	70230	71007	890-894	1.000000	79.5
rpl20-rps12_p3	rpl20-rps12	3	TTGAATGAGCCCCTCGAAAG	CGCCGAGGAACATGTACTAG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.234977	70230	71007	889-893	1.000000	79.5
rpl20-rps12_p4	rpl20-rps12	4	CCCTCGAAAGCTTGATCCAA	GCCGAGGAACATGTACTAGG	20	20	50.0	55.0	57.9	57.5	0.0	19.0	0.624628	70230	71007	878-882	1.000000	76.4
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	GCCGAGGAACATGTACTAGG	20	20	50.0	55.0	57.9	57.5	0.0	19.0	0.624628	70230	71007	888-892	1.000000	76.4
psbT-psbN_p1	psbT-psbN	1	TCGAATCTATGGAAGCATTGGT	ATCCTTTCGAAGAACACGGG	22	20	40.9	50.0	57.6	57.9	0.0	0.0	2.516835	75648	75707	202-202	1.000000	61.4
psbT-psbN_p2	psbT-psbN	2	TCGAATCTATGGAAGCATTGGT	GCAGCCCTCTCAACAACTAA	22	20	40.9	50.0	57.6	57.8	0.0	0.0	2.607077	75648	75707	225-225	1.000000	60.6
psbT-psbN_p3	psbT-psbN	3	TCGAATCTATGGAAGCATTGGT	TTCGAAGAACACGGGGACTA	22	20	40.9	50.0	57.6	58.4	0.0	0.0	2.802418	75648	75707	197-197	1.000000	59.2
psbT-psbN_p4	psbT-psbN	4	CGAATCTATGGAAGCATTGGT	ATCCTTTCGAAGAACACGGG	21	20	42.9	50.0	56.1	57.9	0.0	0.0	2.992343	75648	75707	201-201	1.000000	57.7
psbT-psbN_p5	psbT-psbN	5	CGAATCTATGGAAGCATTGGT	GCAGCCCTCTCAACAACTAA	21	20	42.9	50.0	56.1	57.8	0.0	0.0	3.082585	75648	75707	224-224	1.000000	56.8
petD_p1	petD	1	ACATAACACCACTAGTTGAAGGT	TTCCCTAGATACGCATGTCG	23	20	39.1	50.0	57.4	56.9	0.1	7.8	4.642122	77913	79164	1319-1342	1.000000	45.2
petD_p2	petD	2	ACATAACACCACTAGTTGAAGGT	GCGACTATTCCCTAGATACGC	23	21	39.1	52.4	57.4	57.9	0.1	0.0	4.696319	77913	79164	1326-1349	1.000000	44.8
petD_p3	petD	3	ACATAACACCACTAGTTGAAGGT	CGCATGTCGTGTTATTTCACA	23	21	39.1	42.9	57.4	57.6	0.1	0.0	5.010050	77913	79164	1308-1331	1.000000	42.4
petD_p4	petD	4	CACTAGTTGAAGGTAATTCTCCGA	TTCCCTAGATACGCATGTCG	24	20	41.7	50.0	58.0	56.9	0.0	7.8	5.117391	77913	79164	1310-1333	1.000000	42.4
petD_p5	petD	5	ACCACTAGTTGAAGGTAATTCTCC	TTCCCTAGATACGCATGTCG	24	20	41.7	50.0	57.9	56.9	0.1	7.8	5.131003	77913	79164	1312-1335	1.000000	42.4
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	107368	107643	385-393	1.000000	83.7
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	107368	107643	383-391	1.000000	83.7
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	107368	107643	381-389	1.000000	83.7
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	107368	107643	411-419	1.000000	83.4
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	107368	107643	409-417	1.000000	83.4
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCTGTCGGAAAAAGGA	ACGCTTTTTAGAAAGAGAAGTACG	20	24	45.0	37.5	58.0	57.6	0.0	14.9	4.495010	112732	113621	1006-1031	1.000000	48.4
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCTGTCGGAAAAAGGA	CGCTTTTTAGAAAGAGAAGTACGT	20	24	45.0	37.5	58.0	57.6	0.0	9.8	4.495010	112732	113621	1005-1030	1.000000	48.4
ndhF-rpl32_p3	ndhF-rpl32	3	CGGAAAAAGGAGAAGTCCCA	ACGCTTTTTAGAAAGAGAAGTACG	20	24	50.0	37.5	57.4	57.6	0.0	14.9	5.010927	112732	113621	997-1022	1.000000	44.4
ndhF-rpl32_p4	ndhF-rpl32	4	CGGAAAAAGGAGAAGTCCCA	CGCTTTTTAGAAAGAGAAGTACGT	20	24	50.0	37.5	57.4	57.6	0.0	9.8	5.010927	112732	113621	996-1021	1.000000	44.4
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCTGTCGGAAAAAGGA	CGCTTTTTAGAAAGAGAAGTACG	20	23	45.0	39.1	58.0	56.0	0.0	9.8	5.048873	112732	113621	1005-1030	1.000000	44.4
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	CTCTTAGGAAGCAGTGCGAG	CGGAAACCGTTGAAAAGGAA	20	20	55.0	45.0	58.1	56.9	10.0	1.8	1.219452	114708	114838	231-239	1.000000	74.7
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	CTTAGGAAGCAGTGCGAGAG	CGGAAACCGTTGAAAAGGAA	20	20	55.0	45.0	58.1	56.9	10.0	1.8	1.219452	114708	114838	229-237	1.000000	74.7
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AAACAAGCCGCTATGGTGAA	CGGAAACCGTTGAAAAGGAA	20	20	45.0	45.0	58.1	56.9	0.0	1.8	1.235083	114708	114838	267-275	1.000000	74.0
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AACAAGCCGCTATGGTGAAA	CGGAAACCGTTGAAAAGGAA	20	20	45.0	45.0	58.1	56.9	0.0	1.8	1.235083	114708	114838	266-274	1.000000	74.0
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TTCAAACAAGCCGCTATGGT	CGGAAACCGTTGAAAAGGAA	20	20	45.0	45.0	58.1	56.9	0.0	1.8	1.235083	114708	114838	270-278	1.000000	73.9
ndhE-ndhG_p1	ndhE-ndhG	1	TTTCGGCTCGTGACTAATCC	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	57.7	57.8	0.0	0.0	0.466355	118583	118824	326-339	1.000000	81.8
ndhE-ndhG_p2	ndhE-ndhG	2	TTTCGGCTCGTGACTAATCC	ATTGCTGTGGCTCGTCAATA	20	20	50.0	45.0	57.7	57.6	0.0	4.9	0.690544	118583	118824	327-340	1.000000	80.0
ndhE-ndhG_p3	ndhE-ndhG	3	TTTCGGCTCGTGACTAATCC	AATTGCTGTGGCTCGTCAAT	20	20	50.0	45.0	57.7	58.5	0.0	0.0	0.750913	118583	118824	328-341	1.000000	79.5
ndhE-ndhG_p4	ndhE-ndhG	4	TCGGCTCGTGACTAATCCAT	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	58.6	57.8	0.0	0.0	0.777680	118583	118824	324-337	1.000000	79.3
ndhE-ndhG_p5	ndhE-ndhG	5	CAAGACACATAAGAGCCCGA	TTGCTGTGGCTCGTCAATAA	20	20	50.0	45.0	57.3	57.8	0.0	0.0	0.850355	118583	118824	352-365	1.000000	78.3
ycf1_p1	ycf1	1	TCGATATGGGACGGCTTAGT	TGAATCATCCATTCAAGCCCA	20	21	50.0	42.9	58.0	57.6	0.0	0.0	1.394193	126218	126337	1448-1451	1.000000	70.3
ycf1_p2	ycf1	2	GATAGGGATCCTCCACCACA	TGAATCATCCATTCAAGCCCA	20	21	55.0	42.9	57.9	57.6	12.6	0.0	1.488467	126218	126337	1138-1141	1.000000	69.7
ycf1_p3	ycf1	3	GTGATAGGGATCCTCCACCA	TGAATCATCCATTCAAGCCCA	20	21	55.0	42.9	57.9	57.6	12.6	0.0	1.488467	126218	126337	1140-1143	1.000000	69.7
ycf1_p4	ycf1	4	TGATAGGGATCCTCCACCAC	TGAATCATCCATTCAAGCCCA	20	21	55.0	42.9	57.9	57.6	12.6	0.0	1.488467	126218	126337	1139-1142	1.000000	69.7
ycf1_p5	ycf1	5	CAGATTCGATATGGGACGGC	TGAATCATCCATTCAAGCCCA	20	21	55.0	42.9	58.3	57.6	0.0	0.0	1.670520	126218	126337	1453-1456	1.000000	68.1
rrn5-rrn4.5_p1	rrn5-rrn4.5	1	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	131524	131799	385-393	1.000000	83.7
rrn5-rrn4.5_p2	rrn5-rrn4.5	2	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	20	20	50.0	55.0	58.0	58.0	0.0	8.4	0.083276	131524	131799	383-391	1.000000	83.7
rrn5-rrn4.5_p3	rrn5-rrn4.5	3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.087359	131524	131799	381-389	1.000000	83.7
rrn5-rrn4.5_p4	rrn5-rrn4.5	4	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	131524	131799	411-419	1.000000	83.4
rrn5-rrn4.5_p5	rrn5-rrn4.5	5	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.080545	131524	131799	409-417	1.000000	83.4