primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	rps18	1	TCTTACAACGCAACAACCTCA	AGAAATGGGATTTTCGGTATAGGA	21	24	42.9	37.5	58.1	57.5	0.0	0.0	5.601888	63285	63662	528-530	1.000000	44.7
rps18_p2	rps18	2	TCTTACAACGCAACAACCTCA	TGGGATTTTCGGTATAGGAA	21	20	42.9	40.0	58.1	53.4	0.0	0.0	5.693528	63285	63662	523-525	1.000000	44.7
rps18_p3	rps18	3	CTTACAACGCAACAACCTCA	AGAAATGGGATTTTCGGTATAGGA	20	24	45.0	37.5	56.6	57.5	0.0	0.0	5.919139	63285	63662	527-529	1.000000	44.7
rps18_p4	rps18	4	TCTTACAACGCAACAACCTCA	ATGGGATTTTCGGTATAGGA	21	20	42.9	40.0	58.1	53.1	0.0	0.0	5.942609	63285	63662	524-526	1.000000	44.7
rps18_p5	rps18	5	TCTTACAACGCAACAACCTCA	GAAATGGGATTTTCGGTATAGGA	21	23	42.9	39.1	58.1	56.1	0.0	0.0	5.966664	63285	63662	527-529	1.000000	44.7
clpP_p1	clpP	1	AGCATATGTTACAAGGGCCG	AGGATTAGCCGCTTCAATGG	20	20	50.0	50.0	58.0	58.0	4.3	0.0	0.059809	69405	69524	2004-2004	0.500000	59.5
clpP_p2	clpP	2	CCTGCTGGAGATGGACAATC	AGGATTAGCCGCTTCAATGG	20	20	55.0	50.0	58.0	58.0	2.9	0.0	0.062575	69405	69524	1807-1807	0.500000	59.5
clpP_p3	clpP	3	ATCAACAATTCCGTGGGCTT	AGGATTAGCCGCTTCAATGG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.103772	69405	69524	2797-2797	0.500000	59.2
clpP_p4	clpP	4	CTTGTCCTTGTGCTTGTCCT	AGCACAAGGACAAGCACAAT	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.331065	69405	69524	328-328	0.500000	57.4
clpP_p5	clpP	5	AGCATATGTTACAAGGGCCG	AGCACAAGGACAAGCACAAT	20	20	50.0	45.0	58.0	58.3	4.3	0.0	0.336618	69405	69524	805-805	0.500000	57.3
clpP-psbB_p1	clpP-psbB	1	CCATTGAAGCGGCTAATCCT	AACAGGGTCAGAGGGATCAA	20	20	50.0	50.0	58.0	58.0	1.4	0.0	0.071921	71088	71464	852-867	1.000000	86.1
clpP-psbB_p2	clpP-psbB	2	CCATTGAAGCGGCTAATCCT	ACAGGGTCAGAGGGATCAAA	20	20	50.0	50.0	58.0	58.0	1.4	0.0	0.071921	71088	71464	851-866	1.000000	86.1
clpP-psbB_p3	clpP-psbB	3	CCATTGAAGCGGCTAATCCT	AAAAGCACAAGCCCGAAAAC	20	20	50.0	45.0	58.0	58.1	1.4	0.0	0.092539	71088	71464	1024-1039	1.000000	84.9
clpP-psbB_p4	clpP-psbB	4	CCATTGAAGCGGCTAATCCT	AATGCGCCAAAACCAAAACA	20	20	50.0	40.0	58.0	58.0	1.4	4.2	0.056966	71088	71464	1166-1181	1.000000	84.5
clpP-psbB_p5	clpP-psbB	5	CCATTGAAGCGGCTAATCCT	GCTTGTACCCTTCCGGTTAG	20	20	50.0	55.0	58.0	58.0	1.4	0.0	0.047076	71088	71464	1238-1253	1.000000	84.3
petB-petD_p1	petB-petD	1	TAAGCAAGGCATTTCCGGTC	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.5	57.7	0.0	0.0	0.866133	75466	75660	243-244	1.000000	80.6
petB-petD_p2	petB-petD	2	CAAGGCATTTCCGGTCCTTT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.7	57.7	0.0	0.0	1.068771	75466	75660	239-240	1.000000	79.1
petB-petD_p3	petB-petD	3	GTAAGCAAGGCATTTCCGGT	GTTCAAGTCACACACTCCCA	20	20	50.0	50.0	58.8	57.7	0.0	0.0	1.151670	75466	75660	244-245	1.000000	78.3
petB-petD_p4	petB-petD	4	TAAGCAAGGCATTTCCGGTC	AGTTCAAGTCACACACTCCC	20	20	50.0	50.0	58.5	57.4	0.0	0.0	1.162614	75466	75660	244-245	1.000000	78.2
petB-petD_p5	petB-petD	5	AAGGCATTTCCGGTCCTTTA	GTTCAAGTCACACACTCCCA	20	20	45.0	50.0	57.1	57.7	0.0	0.0	1.216774	75466	75660	238-239	1.000000	78.0
rpl23-trnL-CAA_p1	rpl23-trnL-CAA	1	AAACCGCACTCAAAGGTAGG	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.1	58.0	0.0	5.8	0.115621	86287	86604	1052-1052	0.500000	59.1
rpl23-trnL-CAA_p2	rpl23-trnL-CAA	2	AAACCGCACTCAAAGGTAGG	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	58.1	58.0	0.0	5.8	0.116991	86287	86604	1050-1050	0.500000	59.1
rpl23-trnL-CAA_p3	rpl23-trnL-CAA	3	AAACCGCACTCAAAGGTAGG	GTGATCTGCTGGCGAACTTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.117936	86287	86604	1150-1150	0.500000	59.1
rpl23-trnL-CAA_p4	rpl23-trnL-CAA	4	CCCTGCGGTAATGATTCCTC	GTCTCGCGTGTCTACCATTT	20	20	55.0	50.0	58.1	58.0	0.0	5.8	0.123595	86287	86604	802-802	0.500000	59.0
rpl23-trnL-CAA_p5	rpl23-trnL-CAA	5	CCCTGCGGTAATGATTCCTC	CTCGCGTGTCTACCATTTCA	20	20	55.0	50.0	58.1	58.0	0.0	5.8	0.124966	86287	86604	800-800	0.500000	59.0
trnL-CAA_p1	trnL-CAA	1	CTCGGGATGACATGACGATC	GTGATCTGCTGGCGAACTTA	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.067648	86605	86685	243-243	0.500000	59.5
trnL-CAA_p2	trnL-CAA	2	CTCGGGATGACATGACGATC	TCTGCTGGCGAACTTATTCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.081679	86605	86685	239-239	0.500000	59.3
trnL-CAA_p3	trnL-CAA	3	CTCGGGATGACATGACGATC	CTGCTGGCGAACTTATTCCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.087341	86605	86685	238-238	0.500000	59.3
trnL-CAA_p4	trnL-CAA	4	CTCGGGATGACATGACGATC	ACGGGGTGAGATTTGTCCTA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.110519	86605	86685	545-545	0.500000	59.1
trnL-CAA_p5	trnL-CAA	5	CTCGGGATGACATGACGATC	CTTTCTCGTCGTCTCATGGG	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.142869	86605	86685	666-666	0.500000	58.9
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	AGGGTGAGCTTCAAGAGACT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.030548	86686	87194	1093-1093	0.500000	59.8
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	AGGGTGAGCTTCAAGAGACT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.031919	86686	87194	1095-1095	0.500000	59.7
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AAATGGTAGACACGCGAGAC	TTCGAAGAAGGGGAAGGAGT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.033466	86686	87194	1292-1292	0.500000	59.7
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	TGAAATGGTAGACACGCGAG	TTCGAAGAAGGGGAAGGAGT	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.034837	86686	87194	1294-1294	0.500000	59.7
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	CTCGGGATGACATGACGATC	AGGGTGAGCTTCAAGAGACT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.080221	86686	87194	1218-1218	0.500000	59.4
ycf2_p1	ycf2	1	TGGATTTGACCCCATAAGCG	AGGATTTTCCGCAAAGTGGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.055958	90337	90456	1029-1029	0.500000	59.6
ycf2_p2	ycf2	2	TGGATTTGACCCCATAAGCG	TTTCCGCAAAGTGGTGATGA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.077069	90337	90456	1024-1024	0.500000	59.4
ycf2_p3	ycf2	3	ATATCTTCGCACACGGACAC	AGGATTTTCCGCAAAGTGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.082053	90337	90456	658-658	0.500000	59.3
ycf2_p4	ycf2	4	AATTCGCTCGGATCTATGCC	AGGATTTTCCGCAAAGTGGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.090678	90337	90456	563-563	0.500000	59.3
ycf2_p5	ycf2	5	TCGCTCGGATCTATGCCTAA	AGGATTTTCCGCAAAGTGGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.098986	90337	90456	560-560	0.500000	59.2
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	AAACCCTGTAAATGGCGTGA	GAACCTACGACTTCGCCAAT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.285905	92814	92929	409-409	0.500000	57.7
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	AACCCTGTAAATGGCGTGAA	ATTGAACCTACGACTTCGCC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.285905	92814	92929	411-411	0.500000	57.7
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	AACCCTGTAAATGGCGTGAA	GAACCTACGACTTCGCCAAT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.285905	92814	92929	408-408	0.500000	57.7
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	ACCCTGTAAATGGCGTGAAA	ATTGAACCTACGACTTCGCC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.285905	92814	92929	410-410	0.500000	57.7
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	ACCCTGTAAATGGCGTGAAA	GAACCTACGACTTCGCCAAT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.285905	92814	92929	407-407	0.500000	57.7
trnI-CAU_p1	trnI-CAU	1	AAACCCTGTAAATGGCGTGA	TTTCGAGGCGATCGGAAAAT	20	20	45.0	45.0	57.7	58.0	0.0	18.3	0.298476	92930	93003	615-615	0.500000	57.6
trnI-CAU_p2	trnI-CAU	2	AACCCTGTAAATGGCGTGAA	TTTCGAGGCGATCGGAAAAT	20	20	45.0	45.0	57.7	58.0	0.0	18.3	0.298476	92930	93003	614-614	0.500000	57.6
trnI-CAU_p3	trnI-CAU	3	ACCCTGTAAATGGCGTGAAA	TTTCGAGGCGATCGGAAAAT	20	20	45.0	45.0	57.7	58.0	0.0	18.3	0.298476	92930	93003	613-613	0.500000	57.6
trnI-CAU_p4	trnI-CAU	4	TTGACGAGACAGGAGTGGAA	TTTCGAGGCGATCGGAAAAT	20	20	50.0	45.0	58.3	58.0	0.0	18.3	0.318812	92930	93003	853-853	0.500000	57.4
trnI-CAU_p5	trnI-CAU	5	TTTGACGAGACAGGAGTGGA	TTTCGAGGCGATCGGAAAAT	20	20	50.0	45.0	58.3	58.0	0.0	18.3	0.318812	92930	93003	854-854	0.500000	57.4
trnI-CAU-ndhB_p1	trnI-CAU-ndhB	1	ATTGGCGAAGTCGTAGGTTC	AACGGTGGATATGCAAGCAT	20	20	50.0	45.0	58.0	57.9	2.3	12.7	0.063108	93004	94095	1630-1630	0.500000	59.5
trnI-CAU-ndhB_p2	trnI-CAU-ndhB	2	GGCGAAGTCGTAGGTTCAAT	AACGGTGGATATGCAAGCAT	20	20	50.0	45.0	58.0	57.9	2.3	12.7	0.063108	93004	94095	1627-1627	0.500000	59.5
trnI-CAU-ndhB_p3	trnI-CAU-ndhB	3	GAATGGTTAAAGCGCCCAAC	AACGGTGGATATGCAAGCAT	20	20	50.0	45.0	58.0	57.9	2.3	12.7	0.066338	93004	94095	1655-1655	0.500000	59.5
trnI-CAU-ndhB_p4	trnI-CAU-ndhB	4	ATTGGCGAAGTCGTAGGTTC	AGAAATAACCCCTCACGTGC	20	20	50.0	50.0	58.0	57.9	2.3	11.7	0.115395	93004	94095	1283-1283	0.500000	59.1
trnI-CAU-ndhB_p5	trnI-CAU-ndhB	5	GGCGAAGTCGTAGGTTCAAT	AGAAATAACCCCTCACGTGC	20	20	50.0	50.0	58.0	57.9	2.3	11.7	0.115395	93004	94095	1280-1280	0.500000	59.1
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	105792	106021	345-345	1.000000	84.0
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	105792	106021	343-343	1.000000	84.0
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.0	58.0	8.4	0.0	0.083276	105792	106021	347-347	1.000000	83.9
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	GCTGAGGCATCCTAACAGAC	AGTATCGTCACCGCAGTAGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.087359	105792	106021	345-345	1.000000	83.9
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	CAGCTGAGGCATCCTAACAG	TCCCCTACAGTATCGTCACC	20	20	55.0	55.0	58.0	57.9	8.4	0.0	0.112142	105792	106021	355-355	1.000000	83.6
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GAACGCTTTGCCTTTCCTTC	CTGTCCCGTAATAAACCCGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.107847	106965	107509	869-888	1.000000	86.8
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	ATCCCCGGACTGTAGAACAA	CTGTCCCGTAATAAACCCGG	20	20	50.0	55.0	58.1	58.1	0.7	0.0	0.108984	106965	107509	1023-1042	1.000000	86.4
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GAACGCTTTGCCTTTCCTTC	CCCGTAATAAACCCGGTTGT	20	20	50.0	50.0	57.9	57.9	0.0	2.8	0.164259	106965	107509	865-884	1.000000	86.3
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	CCCCGGACTGTAGAACAAAG	CTGTCCCGTAATAAACCCGG	20	20	55.0	55.0	57.9	58.1	0.7	0.0	0.150731	106965	107509	1021-1040	1.000000	86.1
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CCCGGACTGTAGAACAAAGG	CTGTCCCGTAATAAACCCGG	20	20	55.0	55.0	57.9	58.1	0.7	0.0	0.150731	106965	107509	1020-1039	1.000000	86.1
ycf1-rps15_p1	ycf1-rps15	1	GAACTGTGATCCTTCCGTCC	ACAGTAGTCGTTGCCGTTTT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.067509	113400	113911	1387-1424	1.000000	83.6
ycf1-rps15_p2	ycf1-rps15	2	CTGTGATCCTTCCGTCCAAG	ACAGTAGTCGTTGCCGTTTT	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.071704	113400	113911	1384-1421	1.000000	83.6
ycf1-rps15_p3	ycf1-rps15	3	GAACTGTGATCCTTCCGTCC	AACACCATGCATTGATGGGT	20	20	55.0	45.0	58.0	58.1	0.0	8.9	0.070163	113400	113911	1639-1676	1.000000	83.0
ycf1-rps15_p4	ycf1-rps15	4	GAACTGTGATCCTTCCGTCC	ACCATGCATTGATGGGTGTT	20	20	55.0	45.0	58.0	58.1	0.0	8.9	0.070163	113400	113911	1636-1673	1.000000	83.0
ycf1-rps15_p5	ycf1-rps15	5	CTGTGATCCTTCCGTCCAAG	ACCATGCATTGATGGGTGTT	20	20	55.0	45.0	58.0	58.1	0.0	8.9	0.074359	113400	113911	1633-1670	1.000000	82.9