primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
pbsA-rpl35_p1	pbsA-rpl35	1	GAACCGAGATTCTGGTGGTC	AGACTTGCTGCTTTGCTGTA	20	20	55.0	45.0	58.0	57.7	8.1	0.0	0.272384	6057	6764	1692-1728	1.000000	83.7
pbsA-rpl35_p2	pbsA-rpl35	2	GAACCGAGATTCTGGTGGTC	GCAACAAATGACTGCGTGAA	20	20	55.0	45.0	58.0	57.9	8.1	0.0	0.129769	6057	6764	1388-1388	0.500000	59.0
pbsA-rpl35_p3	pbsA-rpl35	3	ATTGGCAACCAGGTAATGCA	GCAACAAATGACTGCGTGAA	20	20	45.0	45.0	58.1	57.9	16.6	0.0	0.182429	6057	6764	1131-1131	0.500000	58.5
pbsA-rpl35_p4	pbsA-rpl35	4	AACGAACCGAGATTCTGGTG	GCAACAAATGACTGCGTGAA	20	20	50.0	45.0	57.9	57.9	8.1	0.0	0.198515	6057	6764	1391-1391	0.500000	58.4
pbsA-rpl35_p5	pbsA-rpl35	5	AACCCGGATGAAGTTTGTCC	GCAACAAATGACTGCGTGAA	20	20	50.0	45.0	58.1	57.9	0.7	0.0	0.212591	6057	6764	1156-1156	0.500000	58.3
pdhB-petA_p1	pdhB-petA	1	TCTGTGAGGAAATTAGTAGCT	CTTTGCTGTGACACTTGCAC	21	20	38.1	50.0	53.7	58.2	0.0	20.6	5.464858	10781	10844	170-176	1.000000	46.0
pdhB-petA_p2	pdhB-petA	2	TCTGTGAGGAAATTAGTAGCT	TCTTTGCTGTGACACTTGCA	21	20	38.1	45.0	53.7	58.3	0.0	18.2	5.547251	10781	10844	171-177	1.000000	46.0
pdhB-petA_p3	pdhB-petA	3	TTCTGTGAGGAAATTAGTAGCT	CTTTGCTGTGACACTTGCAC	22	20	36.4	50.0	54.6	58.2	0.0	20.6	5.612035	10781	10844	171-177	1.000000	46.0
pdhB-petA_p4	pdhB-petA	4	AGATTCTGTGAGGAAATTAGTAGCT	CTTTGCTGTGACACTTGCAC	25	20	36.0	50.0	57.6	58.2	0.0	20.6	5.599417	10781	10844	174-180	1.000000	45.9
pdhB-petA_p5	pdhB-petA	5	AGATTCTGTGAGGAAATTAGTAGCT	TCTTTGCTGTGACACTTGCA	25	20	36.0	45.0	57.6	58.3	0.0	18.2	5.681810	10781	10844	175-181	1.000000	45.8
ycf58-cemA_p1	ycf58-cemA	1	TTGATGAGAGAGCCAGTTGC	ACCTCTGCATTTGGATCGAG	20	20	50.0	50.0	57.9	57.7	0.0	6.8	0.427362	44152	46519	2550-2550	0.500000	56.6
ycf58-cemA_p2	ycf58-cemA	2	TTGATGAGAGAGCCAGTTGC	CCTCTGCATTTGGATCGAGT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.427362	44152	46519	2549-2549	0.500000	56.6
ycf58-cemA_p3	ycf58-cemA	3	GAGCCAGTTGCCTTCCATAT	ACCTCTGCATTTGGATCGAG	20	20	50.0	50.0	57.6	57.7	0.0	6.8	0.677365	44152	46519	2541-2541	0.500000	54.6
ycf58-cemA_p4	ycf58-cemA	4	GAGCCAGTTGCCTTCCATAT	CCTCTGCATTTGGATCGAGT	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.677365	44152	46519	2540-2540	0.500000	54.6
ycf58-cemA_p5	ycf58-cemA	5	AGAGCCAGTTGCCTTCCATA	CCTCTGCATTTGGATCGAGT	20	20	50.0	50.0	58.7	57.7	0.0	0.0	1.033195	44152	46519	2541-2541	0.500000	51.7
trnMe(cat)_p1	trnMe(cat)	1	CCATCAGCAGTGGCAACTTA	AACACGTATTGGTCAACCGT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.418512	47404	49419	2404-2404	0.500000	56.7
trnMe(cat)_p2	trnMe(cat)	2	CCATCAGCAGTGGCAACTTA	ACACGTATTGGTCAACCGTT	20	20	50.0	45.0	58.2	57.8	0.0	7.3	0.418512	47404	49419	2403-2403	0.500000	56.7
trnMe(cat)_p3	trnMe(cat)	3	CCATCAGCAGTGGCAACTTA	CCCGCTACCCGCTTTAAATA	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.418512	47404	49419	2147-2147	0.500000	56.7
trnMe(cat)_p4	trnMe(cat)	4	AGCGATACAGCAAAGGCATT	ACACGTATTGGTCAACCGTT	20	20	45.0	45.0	58.2	57.8	0.0	7.3	0.488423	47404	49419	2619-2619	0.500000	56.1
trnMe(cat)_p5	trnMe(cat)	5	AGCGATACAGCAAAGGCATT	CCCGCTACCCGCTTTAAATA	20	20	45.0	50.0	58.2	57.8	0.0	0.0	0.488423	47404	49419	2363-2363	0.500000	56.1
cbbX-trnC(gca)_p1	cbbX-trnC(gca)	1	ACGATTTAGTAACTATACAATCAGA	TCTGCCTTACCACTTGGCTA	25	20	28.0	50.0	53.7	58.3	0.0	0.0	9.665558	55311	55400	161-176	1.000000	48.3
cbbX-trnC(gca)_p2	cbbX-trnC(gca)	2	ACGATTTAGTAACTATACAATCAGA	TTGCAATCCTCTGCCTTACC	25	20	28.0	50.0	53.7	57.9	0.0	0.0	9.446065	55311	55400	170-185	1.000000	48.1
cbbX-trnC(gca)_p3	cbbX-trnC(gca)	3	ACGATTTAGTAACTATACAATCAGA	GGATTTGCAATCCTCTGCCT	25	20	28.0	50.0	53.7	58.2	0.0	16.2	9.544458	55311	55400	174-189	1.000000	48.0
cbbX-trnC(gca)_p4	cbbX-trnC(gca)	4	ACGATTTAGTAACTATACAATCAGA	AGGATTTGCAATCCTCTGCC	25	20	28.0	50.0	53.7	58.2	0.0	19.0	9.544458	55311	55400	175-190	1.000000	47.9
cbbX-trnC(gca)_p5	cbbX-trnC(gca)	5	ACGATTTAGTAACTATACAATCAGA	TTTTAGGCGGTACCCAGACT	25	20	28.0	50.0	53.7	58.4	0.0	6.3	9.669179	55311	55400	209-224	1.000000	47.3
ycf80_p1	ycf80	1	ACTAGGCATTGCACCAACAA	AAACAAGGAGGTTGCTCTGG	20	20	45.0	50.0	58.0	58.0	11.4	0.0	0.019162	57876	59282	2161-2177	1.000000	85.6
ycf80_p2	ycf80	2	TTGCACCAACAATACCAGCT	AAACAAGGAGGTTGCTCTGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.019162	57876	59282	2153-2169	1.000000	85.6
ycf80_p3	ycf80	3	GGCATTGCACCAACAATACC	AAACAAGGAGGTTGCTCTGG	20	20	50.0	50.0	58.0	58.0	11.4	0.0	0.021869	57876	59282	2157-2173	1.000000	85.6
ycf80_p4	ycf80	4	ACCAGCTGCTCGACAATTAG	AAACAAGGAGGTTGCTCTGG	20	20	50.0	50.0	58.0	58.0	8.7	0.0	0.028853	57876	59282	2140-2156	1.000000	85.5
ycf80_p5	ycf80	5	CCAGCTGCTCGACAATTAGT	AAACAAGGAGGTTGCTCTGG	20	20	50.0	50.0	58.0	58.0	8.7	0.0	0.028853	57876	59282	2139-2155	1.000000	85.5
psbE-ycf63_p1	psbE-ycf63	1	ACCCTGCTACAAAAAGTGCA	GCAAGAGGAGTCGGTCAATC	20	20	45.0	55.0	57.9	58.4	0.0	0.0	0.419709	84932	85643	944-973	1.000000	84.3
psbE-ycf63_p2	psbE-ycf63	2	ACCCTGCTACAAAAAGTGCA	AGCTGTTCTTACATTGGCGT	20	20	45.0	45.0	57.9	57.8	0.0	0.0	0.250903	84932	85643	1359-1359	0.500000	58.0
psbE-ycf63_p3	psbE-ycf63	3	TGGCTGCTATACCACCTACA	AGCTGTTCTTACATTGGCGT	20	20	50.0	45.0	57.8	57.8	0.0	0.0	0.353462	84932	85643	1937-1937	0.500000	57.2
psbE-ycf63_p4	psbE-ycf63	4	ACCCTGCTACAAAAAGTGCA	TTGGTCGTGTAGGATCATGC	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.375426	84932	85643	1297-1297	0.500000	57.0
psbE-ycf63_p5	psbE-ycf63	5	AAAAAGGACGTTCACCCGTT	AGCTGTTCTTACATTGGCGT	20	20	45.0	45.0	58.2	57.8	0.0	0.0	0.431074	84932	85643	1287-1287	0.500000	56.6
trnP(tgg)-ycf52_p1	trnP(tgg)-ycf52	1	GCTACCAAGCTGCGCTATAT	AGGTATGTTTTGGTATCCACGT	20	22	50.0	40.9	57.9	57.4	9.7	0.0	2.649195	90703	90757	104-106	1.000000	65.7
trnP(tgg)-ycf52_p2	trnP(tgg)-ycf52	2	GCTACCAAGCTGCGCTATAT	TGTTTTGGTATCCACGTTGA	20	20	50.0	40.0	57.9	55.2	9.7	0.0	2.875656	90703	90757	99-101	1.000000	64.2
trnP(tgg)-ycf52_p3	trnP(tgg)-ycf52	3	TTTGAACCTGCGACATCCTG	AGGTATGTTTTGGTATCCACGT	20	22	50.0	40.9	58.5	57.4	0.0	0.0	3.040472	90703	90757	140-142	1.000000	60.8
trnP(tgg)-ycf52_p4	trnP(tgg)-ycf52	4	CAGGATTTGAACCTGCGACA	AGGTATGTTTTGGTATCCACGT	20	22	50.0	40.9	58.5	57.4	0.0	0.0	3.040472	90703	90757	145-147	1.000000	60.6
trnP(tgg)-ycf52_p5	trnP(tgg)-ycf52	5	ATTTGAACCTGCGACATCCT	AGGTATGTTTTGGTATCCACGT	20	22	45.0	40.9	57.5	57.4	0.0	0.0	3.060039	90703	90757	141-143	1.000000	60.6
psaF-psaJ_p1	psaF-psaJ	1	GCAGGACTAACTCCATGCAA	AACGTTGCTTCCTTAAGGCA	20	20	50.0	45.0	57.9	58.0	0.0	17.3	0.138201	92101	92124	1193-1194	1.000000	83.5
psaF-psaJ_p2	psaF-psaJ	2	TTGCAGGACTAACTCCATGC	AACGTTGCTTCCTTAAGGCA	20	20	50.0	45.0	57.9	58.0	0.0	17.3	0.138201	92101	92124	1195-1196	1.000000	83.5
psaF-psaJ_p3	psaF-psaJ	3	GCAGGACTAACTCCATGCAA	CAGGTGCAATGTATTCGGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.150818	92101	92124	985-986	1.000000	83.3
psaF-psaJ_p4	psaF-psaJ	4	CCATGCAAAGAATCAGCTGC	AACGTTGCTTCCTTAAGGCA	20	20	50.0	45.0	58.1	58.0	9.5	17.3	0.101770	92101	92124	1182-1182	0.500000	59.2
psaF-psaJ_p5	psaF-psaJ	5	CCATGCAAAGAATCAGCTGC	CAGGTGCAATGTATTCGGGA	20	20	50.0	50.0	58.1	58.0	9.5	0.0	0.114387	92101	92124	974-974	0.500000	59.1
trnL(tag)-trnN(gtt)_p1	trnL(tag)-trnN(gtt)	1	AGCGTGTCTACCAATTCCAC	CCCCAGGAAGGATTTGAACC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.242130	107032	107200	268-274	1.000000	85.7
trnL(tag)-trnN(gtt)_p2	trnL(tag)-trnN(gtt)	2	AGCGTGTCTACCAATTCCAC	AACCTCCCCAGGAAGGATTT	20	20	50.0	50.0	57.9	58.2	0.0	13.5	0.321221	107032	107200	273-279	1.000000	85.0
trnL(tag)-trnN(gtt)_p3	trnL(tag)-trnN(gtt)	3	CTACCAATTCCACCACGTCC	CCCCAGGAAGGATTTGAACC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.413205	107032	107200	261-267	1.000000	84.6
trnL(tag)-trnN(gtt)_p4	trnL(tag)-trnN(gtt)	4	AATATGCGGACGGAGAGACT	CCCCAGGAAGGATTTGAACC	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.453741	107032	107200	326-332	1.000000	82.7
trnL(tag)-trnN(gtt)_p5	trnL(tag)-trnN(gtt)	5	ATATGCGGACGGAGAGACTT	CCCCAGGAAGGATTTGAACC	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.453741	107032	107200	325-331	1.000000	82.7
ccdA_p1	ccdA	1	AGCGTGTCTACCAATTCCAC	AAGCCTGGTAATTGACGAGA	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.538133	107310	108050	1408-1414	1.000000	73.9
ccdA_p2	ccdA	2	AGCGTGTCTACCAATTCCAC	AGCCTGGTAATTGACGAGAA	20	20	50.0	45.0	57.9	56.6	0.0	0.0	1.538133	107310	108050	1407-1413	1.000000	73.9
ccdA_p3	ccdA	3	GGTTCAAATCCTTCCTGGGG	AAGCCTGGTAATTGACGAGA	20	20	55.0	45.0	58.1	56.6	0.0	0.0	1.590738	107310	108050	1160-1160	1.000000	73.0
ccdA_p4	ccdA	4	GGTTCAAATCCTTCCTGGGG	AGCCTGGTAATTGACGAGAA	20	20	55.0	45.0	58.1	56.6	0.0	0.0	1.590738	107310	108050	1159-1159	1.000000	73.0
ccdA_p5	ccdA	5	AAATCCTTCCTGGGGAGGTT	AGCCTGGTAATTGACGAGAA	20	20	50.0	45.0	58.2	56.6	13.3	0.0	1.669830	107310	108050	1154-1154	1.000000	72.4
ycf27-psbD_p1	ycf27-psbD	1	TGATGCCCAAGTTAGATGGC	GATTCCTACAAGCCGAGCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.081313	117931	118377	1415-1415	0.500000	59.3
ycf27-psbD_p2	ycf27-psbD	2	AACTCACTGTATCCGCACAC	GATTCCTACAAGCCGAGCAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104462	117931	118377	1214-1214	0.500000	59.2
ycf27-psbD_p3	ycf27-psbD	3	TGATGCCCAAGTTAGATGGC	AGCGCATAAAAGAGCTCCTC	20	20	50.0	50.0	57.9	58.1	0.0	11.5	0.110392	117931	118377	1637-1637	0.500000	59.1
ycf27-psbD_p4	ycf27-psbD	4	TGATGCCCAAGTTAGATGGC	GGTCTGATTCCTACAAGCCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.115812	117931	118377	1420-1420	0.500000	59.1
ycf27-psbD_p5	ycf27-psbD	5	AACTCACTGTATCCGCACAC	AGCGCATAAAAGAGCTCCTC	20	20	50.0	50.0	57.9	58.1	0.0	11.5	0.133541	117931	118377	1436-1436	0.500000	58.9
trnQ(ttg)-psb28_p1	trnQ(ttg)-psb28	1	TTTGACTCCGCCATTCGTAG	CCTTTAATGAATTGAATACTCGCCA	20	25	50.0	36.0	58.0	58.0	0.0	0.0	5.031213	126500	126778	349-534	1.000000	46.2
trnQ(ttg)-psb28_p2	trnQ(ttg)-psb28	2	TTTGACTCCGCCATTCGTAG	AATGAATTGAATACTCGCCA	20	20	50.0	35.0	58.0	53.0	0.0	0.0	5.048317	126500	126778	344-529	1.000000	46.2
trnQ(ttg)-psb28_p3	trnQ(ttg)-psb28	3	CTTTGACTCCGCCATTCGTA	CCTTTAATGAATTGAATACTCGCCA	20	25	50.0	36.0	58.0	58.0	0.0	0.0	5.031213	126500	126778	350-535	1.000000	46.1
trnQ(ttg)-psb28_p4	trnQ(ttg)-psb28	4	CTTTGACTCCGCCATTCGTA	ACCTTTAATGAATTGAATACTCGCC	20	25	50.0	36.0	58.0	58.0	0.0	0.0	5.032360	126500	126778	351-536	1.000000	46.1
trnQ(ttg)-psb28_p5	trnQ(ttg)-psb28	5	TTTGACTCCGCCATTCGTAG	ACCTTTAATGAATTGAATACTCGCC	20	25	50.0	36.0	58.0	58.0	0.0	0.0	5.032360	126500	126778	350-535	1.000000	46.1
clpC-rpl19_p1	clpC-rpl19	1	TTTCTACCGCAACAAACCCA	GGCAAAACCAGCGGATTTAC	20	20	45.0	50.0	57.9	58.0	0.0	1.5	0.063433	141856	142854	1861-1866	1.000000	86.9
clpC-rpl19_p2	clpC-rpl19	2	TTTCTACCGCAACAAACCCA	AGGCAAAACCAGCGGATTTA	20	20	45.0	45.0	57.9	58.1	0.0	1.5	0.136360	141856	142854	1862-1867	1.000000	86.3
clpC-rpl19_p3	clpC-rpl19	3	TTTCTACCGCAACAAACCCA	TAGGCAAAACCAGCGGATTT	20	20	45.0	45.0	57.9	58.1	0.0	1.5	0.136360	141856	142854	1863-1868	1.000000	86.3
clpC-rpl19_p4	clpC-rpl19	4	TTTCTACCGCAACAAACCCA	AACCAGCGGATTTACAGTCC	20	20	45.0	50.0	57.9	57.9	0.0	1.5	0.163657	141856	142854	1856-1861	1.000000	86.1
clpC-rpl19_p5	clpC-rpl19	5	GTGCAGCAATACCTGTTCCT	GGCAAAACCAGCGGATTTAC	20	20	50.0	50.0	58.2	58.0	0.0	1.5	0.182816	141856	142854	1768-1773	1.000000	86.0
trnS(gct)-trnD(gtc)_p1	trnS(gct)-trnD(gtc)	1	GCAGCAGATGATGAAGGTCA	TGGGTGCATTAGGAGGAAGA	20	20	50.0	50.0	58.0	58.0	1.6	0.0	0.068690	146943	148592	2767-2773	1.000000	91.5
trnS(gct)-trnD(gtc)_p2	trnS(gct)-trnD(gtc)	2	TGCAGCAGATGATGAAGGTC	TGGGTGCATTAGGAGGAAGA	20	20	50.0	50.0	58.0	58.0	1.6	0.0	0.068690	146943	148592	2768-2774	1.000000	91.5
trnS(gct)-trnD(gtc)_p3	trnS(gct)-trnD(gtc)	3	GCAGCAGATGATGAAGGTCA	GGTGCATTAGGAGGAAGAGC	20	20	50.0	55.0	58.0	58.0	1.6	0.0	0.073688	146943	148592	2765-2771	1.000000	91.4
trnS(gct)-trnD(gtc)_p4	trnS(gct)-trnD(gtc)	4	GCAGCAGATGATGAAGGTCA	GTGCATTAGGAGGAAGAGCC	20	20	50.0	55.0	58.0	58.0	1.6	0.0	0.073688	146943	148592	2770-2770	0.500000	59.4
trnS(gct)-trnD(gtc)_p5	trnS(gct)-trnD(gtc)	5	TGCAGCAGATGATGAAGGTC	GTGCATTAGGAGGAAGAGCC	20	20	50.0	55.0	58.0	58.0	1.6	0.0	0.073688	146943	148592	2771-2771	0.500000	59.4