primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	psbK-psbI	1	CAGGCCGCGGTAAGTTTC	CGTCCAGGATCGTTTGACAA	18	20	61.1	50.0	58.8	58.2	28.7	12.7	3.034457	9885	10520	705-749	1.000000	65.2
psbK-psbI_p2	psbK-psbI	2	CAGGCCGCGGTAAGTTTC	TTTACGTCCAGGATTCCGTC	18	20	61.1	50.0	58.8	57.3	28.7	6.9	3.485401	9885	10520	721-765	1.000000	61.5
psbK-psbI_p3	psbK-psbI	3	CAGGCCGCGGTAAGTTTC	CTTTACGTCCAGGATTCCGT	18	20	61.1	50.0	58.8	57.3	28.7	8.5	3.492686	9885	10520	722-766	1.000000	61.4
psbK-psbI_p4	psbK-psbI	4	CAGGCCGCGGTAAGTTTC	TCTTTACGTCCAGGATTCCG	18	20	61.1	50.0	58.8	57.0	28.7	0.0	3.778678	9885	10520	723-767	1.000000	59.1
psbK-psbI_p5	psbK-psbI	5	CAGGCCGCGGTAAGTTTC	GTCCAGGATCGTTTGACAAGA	18	21	61.1	47.6	58.8	58.0	28.7	0.0	3.865360	9885	10520	704-748	1.000000	58.6
ycf12-trnG-UCC_p1	ycf12-trnG-UCC	1	TAGCAGCCCGTAAAGGAAAC	AGACGAATCACACTTTTACCACT	20	23	50.0	39.1	57.9	58.0	0.0	0.0	3.146241	11569	11707	201-204	1.000000	65.1
ycf12-trnG-UCC_p2	ycf12-trnG-UCC	2	TCGTATCGGGACCACTTGTA	AGACGAATCACACTTTTACCACT	20	23	50.0	39.1	57.9	58.0	0.0	0.0	3.169974	11569	11707	231-234	1.000000	63.6
ycf12-trnG-UCC_p3	ycf12-trnG-UCC	3	GCGCTAATAGTCGTATCGGG	AGACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.1	58.0	0.0	0.0	3.150236	11569	11707	241-244	1.000000	63.4
ycf12-trnG-UCC_p4	ycf12-trnG-UCC	4	CGGGACCACTTGTAATTGCT	AGACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.2	58.0	0.0	0.0	3.221487	11569	11707	225-228	1.000000	63.4
ycf12-trnG-UCC_p5	ycf12-trnG-UCC	5	GGCGCTAATAGTCGTATCGG	AGACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.1	58.0	7.6	0.0	3.150236	11569	11707	242-245	1.000000	63.3
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	ATGGTCGAGTGGTTTAAGGC	CGTTCTTTCAATGACTCCCCT	20	21	50.0	47.6	57.9	57.9	0.0	0.0	1.168777	34586	35470	996-1045	1.000000	77.3
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GGGTTCGAATCCCTCTCTCT	CGTTCTTTCAATGACTCCCCT	20	21	55.0	47.6	58.2	57.9	17.2	0.0	1.276280	34586	35470	936-985	1.000000	76.9
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	CTTGAAAACTGGCATGGTGC	CGTTCTTTCAATGACTCCCCT	20	21	50.0	47.6	58.2	57.9	0.0	0.0	1.272339	34586	35470	970-1019	1.000000	76.7
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	TCAGGAGAGATGGTCGAGTG	CGTTCTTTCAATGACTCCCCT	20	21	55.0	47.6	58.2	57.9	0.0	0.0	1.302922	34586	35470	1005-1054	1.000000	76.2
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	ATGGTCGAGTGGTTTAAGGC	ACCCGTTCTTTCAATGACTCC	20	21	50.0	47.6	57.9	58.2	0.0	0.0	1.329919	34586	35470	999-1048	1.000000	76.0
psbE-petL_p1	psbE-petL	1	GTAATGCTGTGGATGACCCA	GCGGATGTCAAAAAGGCAAA	20	20	50.0	45.0	57.6	57.9	0.0	0.0	0.565804	65766	66984	1337-1353	1.000000	81.3
psbE-petL_p2	psbE-petL	2	GTTCTCCCGTATTCCCAGAC	GCGGATGTCAAAAAGGCAAA	20	20	55.0	45.0	57.4	57.9	0.0	0.0	0.746121	65766	66984	1282-1298	1.000000	80.1
psbE-petL_p3	psbE-petL	3	AGTAATGCTGTGGATGACCC	GCGGATGTCAAAAAGGCAAA	20	20	50.0	45.0	57.3	57.9	0.0	0.0	0.865075	65766	66984	1338-1354	1.000000	78.9
psbE-petL_p4	psbE-petL	4	AGGACGTTCTCCCGTATTCC	GCGGATGTCAAAAAGGCAAA	20	20	55.0	45.0	58.9	57.9	0.0	0.0	1.037827	65766	66984	1287-1303	1.000000	77.8
psbE-petL_p5	psbE-petL	5	TTCTCCCGTATTCCCAGACG	GCGGATGTCAAAAAGGCAAA	20	20	55.0	45.0	58.9	57.9	0.0	0.0	1.038659	65766	66984	1281-1297	1.000000	77.8
rpl23-trnI-CAU_p1	rpl23-trnI-CAU	1	ACTTGATTCTTTAGTCCATCCA	TGGCGAATTCACAGGTTCAA	22	20	36.4	45.0	54.8	58.0	0.0	6.2	5.229303	87464	87702	284-297	1.000000	51.6
rpl23-trnI-CAU_p2	rpl23-trnI-CAU	2	ACTTGATTCTTTAGTCCATCCA	TTGGCGAATTCACAGGTTCA	22	20	36.4	45.0	54.8	58.0	0.0	6.2	5.229303	87464	87702	285-298	1.000000	51.5
rpl23-trnI-CAU_p3	rpl23-trnI-CAU	3	ACTTGATTCTTTAGTCCATCCA	AACGGTTAAAGCACCCAACT	22	20	36.4	45.0	54.8	57.9	0.0	0.0	5.265077	87464	87702	310-323	1.000000	50.6
rpl23-trnI-CAU_p4	rpl23-trnI-CAU	4	ACTTGATTCTTTAGTCCATCCA	TGAACGGTTAAAGCACCCAA	22	20	36.4	45.0	54.8	57.9	0.0	0.0	5.257800	87464	87702	312-325	1.000000	50.5
rpl23-trnI-CAU_p5	rpl23-trnI-CAU	5	ACTTGATTCTTTAGTCCATCCA	CATCCATGGCTGAACGGTTA	22	20	36.4	50.0	54.8	58.0	0.0	10.2	5.240525	87464	87702	322-335	1.000000	50.2
ndhF_p1	ndhF	1	AGGGAGAGATATGTGGACGG	CTTCTTTCCAAAGCAGGGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.003581	108628	110817	2744-2744	0.500000	60.0
ndhF_p2	ndhF	2	GAGAGATATGTGGACGGGGA	CTTCTTTCCAAAGCAGGGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.004278	108628	110817	2741-2741	0.500000	60.0
ndhF_p3	ndhF	3	AGGGAGAGATATGTGGACGG	CGGATCCGTGACAAATGAGT	20	20	55.0	50.0	58.0	58.0	0.0	26.3	0.012650	108628	110817	2667-2667	0.500000	59.9
ndhF_p4	ndhF	4	GAGAGATATGTGGACGGGGA	CGGATCCGTGACAAATGAGT	20	20	55.0	50.0	58.0	58.0	0.0	26.3	0.013347	108628	110817	2664-2664	0.500000	59.9
ndhF_p5	ndhF	5	AGGGAGAGATATGTGGACGG	AATGTTAACGTCGGACCTCG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.015807	108628	110817	2624-2624	0.500000	59.9
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	ACCCCTGCTTTGGAAAGAAG	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	3.2	0.0	0.004784	111784	112832	1871-1871	0.500000	60.0
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	ACCCCTGCTTTGGAAAGAAG	CGATAACGAGAAAATGCCGC	20	20	50.0	50.0	58.0	58.0	3.2	0.0	0.006264	111784	112832	1899-1899	0.500000	59.9
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	ACTCATTTGTCACGGATCCG	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	32.2	0.0	0.013853	111784	112832	1948-1948	0.500000	59.9
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	ACTCATTTGTCACGGATCCG	CGATAACGAGAAAATGCCGC	20	20	50.0	50.0	58.0	58.0	32.2	0.0	0.015333	111784	112832	1976-1976	0.500000	59.9
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	CGAGGTCCGACGTTAACATT	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	6.4	0.0	0.017010	111784	112832	1991-1991	0.500000	59.9
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TCAGCTTTTGAATGCCCACT	AGCGACCGTCATACCTTTTC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.006322	113167	113566	1272-1272	0.500000	59.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GTCTGGACTAGAAACCGCTG	AGCGACCGTCATACCTTTTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.008301	113167	113566	1049-1049	0.500000	59.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	CTCAAGCATCTCGGTTCGAA	AGCGACCGTCATACCTTTTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009679	113167	113566	575-575	0.500000	59.9
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AAGTGAAGCGCATCATACGT	AGCGACCGTCATACCTTTTC	20	20	45.0	50.0	58.0	58.0	19.3	0.0	0.016116	113167	113566	1168-1168	0.500000	59.9
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TCAGCTTTTGAATGCCCACT	TGAATCTGTTGCTCGACTCG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.017740	113167	113566	1669-1669	0.500000	59.9
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	CAAAGTTCCGGGGAATAGGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.056491	113567	114538	1641-1641	0.500000	59.5
ccsA_p2	ccsA	2	GCGGCATTTTCTCGTTATCG	CAAAGTTCCGGGGAATAGGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.057971	113567	114538	1613-1613	0.500000	59.5
ccsA_p3	ccsA	3	CTCAAGCATCTCGGTTCGAA	ACTTTTTCGCTGACGTCACT	20	20	50.0	45.0	58.0	58.1	0.0	15.8	0.067776	113567	114538	1692-1692	0.500000	59.5
ccsA_p4	ccsA	4	GCGGCATTTTCTCGTTATCG	ACTTTTTCGCTGACGTCACT	20	20	50.0	45.0	58.0	58.1	0.0	15.8	0.069256	113567	114538	1664-1664	0.500000	59.4
ccsA_p5	ccsA	5	CTCAAGCATCTCGGTTCGAA	ACCTTACACCCCAAAACCAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.079915	113567	114538	1440-1440	0.500000	59.4
ccsA-ndhD_p1	ccsA-ndhD	1	CTGAGCTATGCAACTCCGTT	TTGGCTTCTCTCGCATTACC	20	20	50.0	50.0	58.0	58.0	11.0	0.0	0.039911	114539	114984	1349-1349	0.500000	59.7
ccsA-ndhD_p2	ccsA-ndhD	2	CGAGTCGAGCAACAGATTCA	TTGGCTTCTCTCGCATTACC	20	20	50.0	50.0	58.0	58.0	6.7	0.0	0.041563	114539	114984	1260-1260	0.500000	59.7
ccsA-ndhD_p3	ccsA-ndhD	3	GGGGGTCAATCTATCGGGTA	TTGGCTTCTCTCGCATTACC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.043983	114539	114984	861-861	0.500000	59.6
ccsA-ndhD_p4	ccsA-ndhD	4	CTGAGCTATGCAACTCCGTT	TCGCATTACCAGGAATGAGC	20	20	50.0	50.0	58.0	58.0	11.0	6.8	0.062177	114539	114984	1339-1339	0.500000	59.5
ccsA-ndhD_p5	ccsA-ndhD	5	CGAGTCGAGCAACAGATTCA	TCGCATTACCAGGAATGAGC	20	20	50.0	50.0	58.0	58.0	6.7	6.8	0.063829	114539	114984	1250-1250	0.500000	59.5
ndhD_p1	ndhD	1	GGGGGTCAATCTATCGGGTA	TTGCCCAACGGATTTCTTGA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.031514	114985	116493	2243-2243	0.500000	59.7
ndhD_p2	ndhD	2	GGGGGTCAATCTATCGGGTA	TGTACCCAATGCGTAAGAGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.038349	114985	116493	2374-2374	0.500000	59.7
ndhD_p3	ndhD	3	GGGGGTCAATCTATCGGGTA	CCCACGGACGTATTGGAAAT	20	20	55.0	50.0	58.0	58.0	0.0	10.4	0.052488	114985	116493	2350-2350	0.500000	59.6
ndhD_p4	ndhD	4	CCCTATTCCCCGGAACTTTG	TTGCCCAACGGATTTCTTGA	20	20	55.0	45.0	57.9	58.0	0.7	0.0	0.064489	114985	116493	1973-1973	0.500000	59.5
ndhD_p5	ndhD	5	GAAGCGTGGGGATCTTACTG	TTGCCCAACGGATTTCTTGA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.070382	114985	116493	2410-2410	0.500000	59.4
ndhG_p1	ndhG	1	CTAACCGTGTTTCGACTCGT	TCGAAGTTATGGGCAACGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.046686	117812	118405	1618-1618	0.500000	59.6
ndhG_p2	ndhG	2	CGATACGAGTCGATCTGCTG	TCGAAGTTATGGGCAACGAG	20	20	55.0	50.0	58.1	58.0	21.5	0.0	0.060687	117812	118405	1818-1818	0.500000	59.5
ndhG_p3	ndhG	3	CTAACCGTGTTTCGACTCGT	TGGTCGCATCCATTTCGAAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.079061	117812	118405	1480-1480	0.500000	59.4
ndhG_p4	ndhG	4	CTAACCGTGTTTCGACTCGT	GCTATGTGTGATGGCAAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.084790	117812	118405	1063-1063	0.500000	59.3
ndhG_p5	ndhG	5	CAACCCCCTCTCCACAAAAA	TCGAAGTTATGGGCAACGAG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.085237	117812	118405	1528-1528	0.500000	59.3
ndhI_p1	ndhI	1	TCCTTGCCATCACACATAGC	TTATCTGGGTGGGTGGGATT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048287	118581	119150	1072-1072	0.500000	59.6
ndhI_p2	ndhI	2	TCCTTGCCATCACACATAGC	TTTATCTGGGTGGGTGGGAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048287	118581	119150	1073-1073	0.500000	59.6
ndhI_p3	ndhI	3	TCCTTGCCATCACACATAGC	AAGTTTCTTTTGCCCGTTGC	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.108097	118581	119150	855-855	0.500000	59.1
ndhI_p4	ndhI	4	TGCCATCACACATAGCGAAA	TTATCTGGGTGGGTGGGATT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.112078	118581	119150	1068-1068	0.500000	59.1
ndhI_p5	ndhI	5	TGCCATCACACATAGCGAAA	TTTATCTGGGTGGGTGGGAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.112078	118581	119150	1069-1069	0.500000	59.1
ndhH_p1	ndhH	1	ATCGCACGTAGGGATACAGA	GCGTAGGTTTGAACACTTGC	20	20	50.0	50.0	58.0	58.0	13.2	0.0	0.055358	121475	122659	2414-2414	0.500000	59.6
ndhH_p2	ndhH	2	TCCTGCCATTAGAAGACCCA	GCGTAGGTTTGAACACTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067000	121475	122659	2301-2301	0.500000	59.5
ndhH_p3	ndhH	3	ATTATTCCCAACGGACCTGC	GCGTAGGTTTGAACACTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.085196	121475	122659	2072-2072	0.500000	59.3
ndhH_p4	ndhH	4	TTTGTAGGTTTCGGTGCCAA	GCGTAGGTTTGAACACTTGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.086646	121475	122659	1875-1875	0.500000	59.3
ndhH_p5	ndhH	5	ATCGCACGTAGGGATACAGA	ATTGTCGTTCCGCCAGATAC	20	20	50.0	50.0	58.0	58.1	13.2	0.0	0.087011	121475	122659	1840-1840	0.500000	59.3
rps15_p1	rps15	1	CAACTCCTCCTACGCGAAAA	CTATCACGCGCTTATCTCCC	20	20	50.0	55.0	57.9	58.0	15.9	5.7	0.090575	122766	123035	1535-1535	0.500000	59.3
rps15_p2	rps15	2	CAACTCCTCCTACGCGAAAA	TATCTCCCGCAGTGGTAGAG	20	20	50.0	55.0	57.9	58.0	15.9	1.3	0.091887	122766	123035	1523-1523	0.500000	59.3
rps15_p3	rps15	3	CAACTCCTCCTACGCGAAAA	TCTCCTACTTCCGAGACGTC	20	20	50.0	55.0	57.9	58.0	15.9	7.7	0.095902	122766	123035	1465-1465	0.500000	59.2
rps15_p4	rps15	4	ATCATTCGCATACCGGTAGC	CTATCACGCGCTTATCTCCC	20	20	50.0	55.0	57.9	58.0	20.9	5.7	0.096314	122766	123035	1506-1506	0.500000	59.2
rps15_p5	rps15	5	ATCATTCGCATACCGGTAGC	TATCTCCCGCAGTGGTAGAG	20	20	50.0	55.0	57.9	58.0	20.9	1.3	0.097626	122766	123035	1494-1494	0.500000	59.2
ycf1_p1	ycf1	1	GGGGGTGTTTTTGATCCACT	ACCGGGATTGGATTTTCGAG	20	20	50.0	50.0	58.0	58.0	0.0	6.3	0.048068	126726	126845	1117-1117	0.500000	59.6
ycf1_p2	ycf1	2	ACGAACATCACTCTCCATGC	ACCGGGATTGGATTTTCGAG	20	20	50.0	50.0	58.0	58.0	0.0	6.3	0.054123	126726	126845	779-779	0.500000	59.6
ycf1_p3	ycf1	3	CCCTTCACAAGTCTACGACG	ACCGGGATTGGATTTTCGAG	20	20	55.0	50.0	58.0	58.0	0.0	6.3	0.059845	126726	126845	892-892	0.500000	59.5
ycf1_p4	ycf1	4	GGGGATCGATGGAGAAAACC	ACCGGGATTGGATTTTCGAG	20	20	55.0	50.0	58.0	58.0	0.5	6.3	0.069683	126726	126845	552-552	0.500000	59.4
ycf1_p5	ycf1	5	GGGGGTGTTTTTGATCCACT	GGAACTCTTGTGGTGGGTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.077047	126726	126845	1386-1386	0.500000	59.4
chlN_p1	chlN	1	CGCAACCCTACCCAATCTAC	TAGGGAAAAGGCGCATACAC	20	20	55.0	50.0	58.1	58.0	0.0	10.8	0.083215	128508	129875	2102-2102	0.500000	59.3
chlN_p2	chlN	2	CGTTTGTAGAGCGGAAGTCA	TAGGGAAAAGGCGCATACAC	20	20	50.0	50.0	57.9	58.0	0.0	10.8	0.093006	128508	129875	2014-2014	0.500000	59.3
chlN_p3	chlN	3	TGAGAACCGTTTGTAGAGCG	TAGGGAAAAGGCGCATACAC	20	20	50.0	50.0	57.9	58.0	0.0	10.8	0.093006	128508	129875	2021-2021	0.500000	59.3
chlN_p4	chlN	4	GGTCCGTGGCTAATATCGAC	TAGGGAAAAGGCGCATACAC	20	20	55.0	50.0	57.9	58.0	0.0	10.8	0.094420	128508	129875	2129-2129	0.500000	59.2
chlN_p5	chlN	5	CGCAACCCTACCCAATCTAC	GGTTAGGGAAAAGGCGCATA	20	20	55.0	50.0	58.1	58.0	0.0	10.8	0.097536	128508	129875	2105-2105	0.500000	59.2
chlL_p1	chlL	1	GCTATCCCCTCCAGATCCAT	AGGGAGAGATATGTGGACGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.038520	129947	130828	1377-1377	0.500000	59.7
chlL_p2	chlL	2	GCTATCCCCTCCAGATCCAT	GAGAGATATGTGGACGGGGA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.039216	129947	130828	1374-1374	0.500000	59.7
chlL_p3	chlL	3	CTCCAACTTGGGTGCTATCC	AGGGAGAGATATGTGGACGG	20	20	55.0	55.0	58.0	58.0	8.0	0.0	0.046628	129947	130828	1390-1390	0.500000	59.6
chlL_p4	chlL	4	CTCCAACTTGGGTGCTATCC	GAGAGATATGTGGACGGGGA	20	20	55.0	55.0	58.0	58.0	8.0	0.0	0.047324	129947	130828	1387-1387	0.500000	59.6
chlL_p5	chlL	5	GCTATCCCCTCCAGATCCAT	CAAGACGTTAAGCCTCCTCC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.047555	129947	130828	1701-1701	0.500000	59.6