primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	78	461	451-556	0.666667	70.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	78	461	454-559	0.666667	70.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	78	461	465-570	0.666667	70.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	78	461	464-569	0.666667	70.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	78	461	466-571	0.666667	70.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4356	5186	1021-1024	1.000000	82.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	AAAAAGGCGCTCAACCTACA	20	20	55.0	45.0	57.9	58.0	0.0	7.6	0.104626	4356	5186	1020-1023	1.000000	82.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4356	5186	969-972	1.000000	81.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGATGGTATGGACGAATCCC	CCTTGAAAAAGGCGCTCAAC	20	20	55.0	50.0	57.9	58.2	0.0	7.6	0.309329	4356	5186	1025-1028	1.000000	80.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGATGGTATGGACGAATCCC	CTTGAAAAAGGCGCTCAACC	20	20	55.0	50.0	57.9	58.2	0.0	7.6	0.309329	4356	5186	1024-1027	1.000000	80.8
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	10251	10953	778-783	1.000000	67.4
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	10251	10953	781-786	1.000000	67.2
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	10251	10953	784-789	1.000000	66.7
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	10251	10953	787-792	1.000000	66.5
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	10251	10953	782-787	1.000000	65.0
atpA-atpF_p1	atpA-atpF	1	GTCGCCTACTTGAAGTACGG	GCATGTTTGGCGCGATAAAA	20	20	55.0	45.0	58.1	58.4	13.2	12.1	0.471192	12768	12837	206-216	1.000000	80.4
atpA-atpF_p2	atpA-atpF	2	CCGTCGCCTACTTGAAGTAC	GCATGTTTGGCGCGATAAAA	20	20	55.0	45.0	58.1	58.4	0.0	12.1	0.471192	12768	12837	208-218	1.000000	80.3
atpA-atpF_p3	atpA-atpF	3	TACTAATCTCGTCGGCTCGA	GCATGTTTGGCGCGATAAAA	20	20	50.0	45.0	57.8	58.4	0.0	12.1	0.606317	12768	12837	126-136	1.000000	79.8
atpA-atpF_p4	atpA-atpF	4	GCTCAATACGTTCGCGGATA	GCATGTTTGGCGCGATAAAA	20	20	50.0	45.0	58.2	58.4	6.1	12.1	0.614222	12768	12837	150-160	1.000000	79.6
atpA-atpF_p5	atpA-atpF	5	GTCGCCTACTTGAAGTACGG	TGTTTGACCAACGAGTTGCA	20	20	55.0	45.0	58.1	58.5	13.2	10.2	0.640350	12768	12837	252-262	1.000000	78.3
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	29463	30811	1511-1513	1.000000	69.4
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	29463	30811	1450-1452	1.000000	67.9
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	29463	30811	1511-1513	1.000000	65.3
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	57.6	56.1	0.0	0.0	2.280835	29463	30811	1450-1452	1.000000	63.7
petN-psbM_p5	petN-psbM	5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.1	57.6	0.0	0.0	2.300208	29463	30811	1512-1514	1.000000	63.5
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	TTGGATTTTGATCCGTCGGG	TGGCAATATGTCTACGCTGG	20	20	50.0	50.0	58.3	57.8	12.3	0.0	0.493710	32180	32643	560-594	1.000000	80.3
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	TTGGATTTTGATCCGTCGGG	GGCAATATGTCTACGCTGGT	20	20	50.0	50.0	58.3	57.8	12.3	0.0	0.495261	32180	32643	559-593	1.000000	80.3
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	TTGGATTTTGATCCGTCGGG	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.3	58.4	12.3	0.0	0.606264	32180	32643	581-615	1.000000	79.4
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	TTGGATTTTGATCCGTCGGG	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.3	57.6	12.3	0.0	0.629941	32180	32643	580-614	1.000000	79.2
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	TTGGATTTTGATCCGTCGGG	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.3	58.4	12.3	0.0	0.667221	32180	32643	614-648	1.000000	78.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	32860	33618	862-893	1.000000	79.6
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32860	33618	841-872	1.000000	79.5
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	32860	33618	841-872	1.000000	79.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	32860	33618	820-851	1.000000	79.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	32860	33618	838-869	1.000000	79.0
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.0	57.8	0.0	1.1	0.185568	38557	39205	796-803	1.000000	82.7
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.395214	38557	39205	792-799	1.000000	81.1
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.530110	38557	39205	795-802	1.000000	80.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.8	57.8	0.0	1.1	0.983632	38557	39205	794-801	1.000000	76.3
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	CTTCTCCTGATGGTTGGTCG	GGGAGAAAAGAGGATAGGGGA	20	21	55.0	52.4	58.0	57.9	0.0	0.0	1.077645	38557	39205	868-875	1.000000	75.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTTGCTAATCGGGGATA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	56.8	57.8	0.0	0.0	1.441190	53178	54219	1075-1186	1.000000	74.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTCCGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	19	20	57.9	50.0	58.3	57.8	0.0	0.0	1.489381	53178	54219	1081-1192	1.000000	74.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGCCCTTTGCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	59.4	57.8	0.0	0.0	1.573560	53178	54219	1083-1194	1.000000	73.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGCTAATCGGGGATAA	AGAAGGTCTACGGTTCGAGT	21	20	47.6	50.0	57.5	57.8	0.0	0.0	1.699406	53178	54219	1075-1186	1.000000	72.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTTGCTAATCGGGGATA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	56.8	57.5	0.0	0.0	1.784962	53178	54219	1074-1185	1.000000	71.9
rbcL-accD_p1	rbcL-accD	1	GAGGCTAGCAAATGGAGTCC	AGATCGAATTGAACCACCGT	20	20	55.0	45.0	58.0	57.2	18.2	0.0	0.802119	59497	60303	876-931	1.000000	77.6
rbcL-accD_p2	rbcL-accD	2	GCAGCAGTGGATACTTTGGA	AGATCGAATTGAACCACCGT	20	20	50.0	45.0	57.9	57.2	0.0	0.0	0.876396	59497	60303	807-862	1.000000	77.0
rbcL-accD_p3	rbcL-accD	3	TTGAATTCGCAGCAGTGGAT	AGATCGAATTGAACCACCGT	20	20	45.0	45.0	58.2	57.2	0.0	0.0	0.937445	59497	60303	815-870	1.000000	76.5
rbcL-accD_p4	rbcL-accD	4	TCGCAGCAGTGGATACTTTG	AGATCGAATTGAACCACCGT	20	20	50.0	45.0	58.3	57.2	0.0	0.0	1.036895	59497	60303	809-864	1.000000	75.7
rbcL-accD_p5	rbcL-accD	5	GAGTCCTGAACTAGCTGCTG	AGATCGAATTGAACCACCGT	20	20	55.0	45.0	57.7	57.2	13.4	0.0	1.054638	59497	60303	862-917	1.000000	75.6
accD_p1	accD	1	TCTTCCGAGAAGAAATGAAA	AAAAATACCTGCTCGCCAGA	20	20	35.0	45.0	52.1	57.5	12.2	4.7	6.350832	60304	62031	1888-1888	0.666667	26.7
accD_p2	accD	2	TCTTCCGAGAAGAAATGAAA	AAAATACCTGCTCGCCAGAA	20	20	35.0	45.0	52.1	57.5	12.2	4.7	6.350832	60304	62031	1887-1887	0.666667	26.7
accD_p3	accD	3	TCTTCCGAGAAGAAATGAAA	AAATACCTGCTCGCCAGAAA	20	20	35.0	45.0	52.1	57.5	12.2	4.7	6.350832	60304	62031	1886-1886	0.666667	26.7
accD_p4	accD	4	TCTTCCGAGAAGAAATGAAA	AATACCTGCTCGCCAGAAAA	20	20	35.0	45.0	52.1	57.5	12.2	4.7	6.350832	60304	62031	1885-1885	0.666667	26.7
accD_p5	accD	5	TCTTCCGAGAAGAAATGAAA	ATACCTGCTCGCCAGAAAAA	20	20	35.0	45.0	52.1	57.5	12.2	4.7	6.350832	60304	62031	1884-1884	0.666667	26.7
rpl33-rps18_p1	rpl33-rps18	1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.790741	72159	72578	512-593	1.000000	78.2
rpl33-rps18_p2	rpl33-rps18	2	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	72159	72578	511-592	1.000000	75.8
rpl33-rps18_p3	rpl33-rps18	3	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	57.2	57.7	0.0	0.0	1.092932	72159	72578	513-594	1.000000	75.8
rpl33-rps18_p4	rpl33-rps18	4	CGACACAATACGCCTAGTCG	ATTCGATCCCCCGATTGGAT	20	20	55.0	50.0	58.5	58.6	0.0	0.0	1.173970	72159	72578	509-590	1.000000	75.2
rpl33-rps18_p5	rpl33-rps18	5	AGAATCGACACAATACGCCT	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.216072	72159	72578	517-598	1.000000	74.8
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	ACAATTGACACGACTGAAAAAGA	20	23	50.0	34.8	58.0	57.2	0.0	13.6	3.819229	120495	120603	238-242	1.000000	52.1
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	ACAATTGACACGACTGAAAAAGA	20	23	50.0	34.8	58.0	57.2	0.0	13.6	3.820600	120495	120603	237-241	1.000000	52.1
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	TAGACACGCTGCTCTTAGGA	ACAATTGACACGACTGAAAAAGA	20	23	50.0	34.8	57.9	57.2	0.0	13.6	3.935676	120495	120603	228-232	1.000000	51.3
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GGTAGACACGCTGCTCTTAG	ACAATTGACACGACTGAAAAAGA	20	23	55.0	34.8	57.8	57.2	0.0	13.6	4.007694	120495	120603	230-234	1.000000	50.7
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTAGACACGCTGCTCTTAGG	ACAATTGACACGACTGAAAAAGA	20	23	55.0	34.8	57.8	57.2	0.0	13.6	4.007694	120495	120603	229-233	1.000000	50.7
ccsA-ndhD_p1	ccsA-ndhD	1	TTCCGCAATTGTCGCTTCTA	GTTGACCGGGTTGAAGCTAT	20	20	45.0	50.0	57.9	57.9	7.4	6.3	0.196926	121573	121843	431-434	1.000000	80.6
ccsA-ndhD_p2	ccsA-ndhD	2	TTCCGCAATTGTCGCTTCTA	ATCAGTTGACCGGGTTGAAG	20	20	45.0	50.0	57.9	57.8	7.4	6.3	0.272505	121573	121843	435-438	1.000000	80.0
ccsA-ndhD_p3	ccsA-ndhD	3	CGCAATTGTCGCTTCTATCG	GTTGACCGGGTTGAAGCTAT	20	20	50.0	50.0	57.7	57.9	7.4	6.3	0.379627	121573	121843	428-431	1.000000	79.2
ccsA-ndhD_p4	ccsA-ndhD	4	TCCGCAATTGTCGCTTCTAT	GTTGACCGGGTTGAAGCTAT	20	20	45.0	50.0	57.7	57.9	7.4	6.3	0.418953	121573	121843	430-433	1.000000	78.9
ccsA-ndhD_p5	ccsA-ndhD	5	CGCAATTGTCGCTTCTATCG	ATCAGTTGACCGGGTTGAAG	20	20	50.0	50.0	57.7	57.8	7.4	6.3	0.455206	121573	121843	432-435	1.000000	78.6
ndhG-ndhI_p1	ndhG-ndhI	1	TCCCAACGAAAAGGCAGAAT	GTCGTTTACCAATGTCAGTAGT	20	22	45.0	40.9	57.7	56.1	0.0	0.0	4.144548	125079	125517	599-631	1.000000	51.0
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAACGAAAAGGCAGAAT	GTCGTTTACCAATGTCAGTAGTTG	20	24	45.0	41.7	57.7	58.1	0.0	0.0	4.373836	125079	125517	599-631	1.000000	49.1
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAACGAAAAGGCAGAAT	TCGTTTACCAATGTCAGTAGTTGA	20	24	45.0	37.5	57.7	58.2	0.0	0.0	4.441262	125079	125517	598-630	1.000000	48.6
ndhG-ndhI_p4	ndhG-ndhI	4	TCCCAACGAAAAGGCAGAAT	CGTTTACCAATGTCAGTAGTTGA	20	23	45.0	39.1	57.7	56.8	0.0	0.0	4.453074	125079	125517	597-629	1.000000	48.5
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAACGAAAAGGCAGAAT	TCGTTTACCAATGTCAGTAGTTG	20	23	45.0	39.1	57.7	56.8	0.0	0.0	4.453074	125079	125517	598-630	1.000000	48.5
ycf1_p1	ycf1	1	TGTCAGTGAATAATTTGTCCAAGC	ACCTTTCAAAATCAAAAAGAAATCCA	24	26	37.5	26.9	58.0	57.0	0.0	0.0	11.045820	132736	132855	170-170	1.000000	44.2
ycf1_p2	ycf1	2	TGTCAGTGAATAATTTGTCCAAGC	ACAACCTTTCAAAATCAAAAAGA	24	23	37.5	26.1	58.0	53.8	0.0	0.0	11.226818	132736	132855	173-173	1.000000	44.2
ycf1_p3	ycf1	3	TGTCAGTGAATAATTTGTCCAAGC	ACAACCTTTCAAAATCAAAAAGAA	24	24	37.5	25.0	58.0	54.6	0.0	0.0	11.454160	132736	132855	173-173	1.000000	44.2
ycf1_p4	ycf1	4	TGTCAGTGAATAATTTGTCCAAGC	AACCTTTCAAAATCAAAAAGAAATCCA	24	27	37.5	25.9	58.0	57.5	0.0	0.0	11.472677	132736	132855	171-171	1.000000	44.2
ycf1_p5	ycf1	5	TGTCAGTGAATAATTTGTCCAAGC	AACAACCTTTCAAAATCAAAAAGA	24	24	37.5	25.0	58.0	54.6	0.0	0.0	11.454160	132736	132855	174-174	1.000000	44.1