primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTACCAACGGTATGGACGA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	57.9	58.0	10.6	7.6	0.151611	4419	5009	746-758	1.000000	81.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4419	5009	681-693	1.000000	81.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4419	5009	680-692	1.000000	81.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	0.0	7.6	0.290178	4419	5009	695-707	1.000000	80.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4419	5009	690-702	1.000000	80.3
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	ATTAGCAATCCGCCGCTTTA	GATGGGAAAATTTGCGTCCA	20	20	45.0	45.0	58.0	57.0	0.0	9.1	1.066296	8033	9743	1807-1835	1.000000	73.0
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	ACGGAAAGAGAGGGATTCGA	GATGGGAAAATTTGCGTCCA	20	20	50.0	45.0	58.2	57.0	0.0	9.1	1.179437	8033	9743	1856-1884	1.000000	72.0
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	ATTAGCAATCCGCCGCTTTA	GATGGGAAAATTTGCGTCCAA	20	21	45.0	42.9	58.0	57.7	0.0	9.1	1.349692	8033	9743	1807-1835	1.000000	70.7
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	ATTAGCAATCCGCCGCTTTA	AGATGGGAAAATTTGCGTCC	20	20	45.0	45.0	58.0	56.7	0.0	0.0	1.364130	8033	9743	1808-1836	1.000000	70.6
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	GCTTTAGTCCACTCAGCCAT	GATGGGAAAATTTGCGTCCA	20	20	50.0	45.0	57.6	57.0	0.9	9.1	1.438038	8033	9743	1793-1821	1.000000	70.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110548	26463	27664	1357-1367	1.000000	79.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGTCAAGCCCTGATCAATGA	TGGGGAAAAAGGGATTTGCA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.137649	26463	27664	1345-1355	1.000000	79.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CGTCAAGCCCTGATCAATGA	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.354003	26463	27664	1375-1385	1.000000	78.0
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.4	58.1	0.0	0.0	0.476375	26463	27664	1367-1377	1.000000	77.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AAAGTTCTTCCGTCAAGCCC	TGGGGAAAAAGGGATTTGCA	20	20	50.0	45.0	58.4	57.9	0.0	0.0	0.503477	26463	27664	1355-1365	1.000000	76.8
petN-psbM_p1	petN-psbM	1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTCT	20	21	45.0	38.1	58.0	55.9	0.0	0.0	3.137832	28697	29347	804-806	1.000000	55.7
petN-psbM_p2	petN-psbM	2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCATTGTTCATTCT	20	21	50.0	38.1	57.8	55.9	0.0	0.0	3.322916	28697	29347	810-812	1.000000	54.2
petN-psbM_p3	petN-psbM	3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCATTGTTCATTCT	20	21	50.0	38.1	57.6	55.9	0.0	0.0	3.482651	28697	29347	750-752	1.000000	53.0
petN-psbM_p4	petN-psbM	4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCATTGTTCATTC	20	20	45.0	40.0	58.0	54.3	0.0	0.0	3.717181	28697	29347	804-806	1.000000	51.1
petN-psbM_p5	petN-psbM	5	TTGCTTGGGCTGCTTTAATG	TGCTACTGCATTGTTCATTCT	20	21	45.0	38.1	57.3	55.9	0.0	0.0	3.879185	28697	29347	802-804	1.000000	49.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47343	48103	795-820	1.000000	82.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47343	48103	803-828	1.000000	82.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47343	48103	827-852	1.000000	81.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	47343	48103	835-860	1.000000	81.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	47343	48103	818-843	1.000000	80.7
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CGGTCCTTCACTAATTGGGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.235670	51572	53147	1685-1723	1.000000	80.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCGGTCCTTCACTAATTGGG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.235670	51572	53147	1686-1724	1.000000	80.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCTTCACTAATTGGGGCCAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	4.4	0.0	0.427413	51572	53147	1681-1719	1.000000	79.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGTCCTTCACTAATTGGGGC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.433315	51572	53147	1684-1722	1.000000	79.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GTCCTTCACTAATTGGGGCC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.433315	51572	53147	1683-1721	1.000000	79.3
accD_p1	accD	1	CGGGATTGGGATTGGGAAAT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.113614	59269	60765	2483-2668	1.000000	84.7
accD_p2	accD	2	TTCGTTCAAATAGGGGGCAG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.149787	59269	60765	2023-2142	1.000000	84.5
accD_p3	accD	3	TGGACCCCTCCAATGTACTT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	26.9	0.080750	59269	60765	2582-2582	0.166667	46.0
accD_p4	accD	4	TGGACCCCTCCAATGTACTT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.9	57.9	0.0	26.8	0.157284	59269	60765	2588-2588	0.166667	45.4
accD_p5	accD	5	TGGACCCCTCCAATGTACTT	GCATAACAGCGCTTTCACAG	20	20	50.0	50.0	57.9	58.1	0.0	7.5	0.166427	59269	60765	2751-2751	0.166667	45.3
rps12-clpP_p1	rps12-clpP	1	GTACATGTTCCTCGACGCTG	ACTTTTATGGACGCAAGCGA	20	20	55.0	45.0	58.7	58.2	2.3	22.3	0.927500	70629	71117	691-698	1.000000	75.1
rps12-clpP_p2	rps12-clpP	2	AGTACATGTTCCTCGACGCT	ACTTTTATGGACGCAAGCGA	20	20	50.0	45.0	58.8	58.2	2.3	22.3	1.021883	70629	71117	692-699	1.000000	74.4
rps12-clpP_p3	rps12-clpP	3	TACATGTTCCTCGACGCTGA	ACTTTTATGGACGCAAGCGA	20	20	50.0	45.0	58.8	58.2	2.3	22.3	1.026224	70629	71117	690-697	1.000000	74.3
rps12-clpP_p4	rps12-clpP	4	TAGTACATGTTCCTCGACGC	ACTTTTATGGACGCAAGCGA	20	20	50.0	45.0	57.2	58.2	2.3	22.3	1.035052	70629	71117	693-700	1.000000	74.2
rps12-clpP_p5	rps12-clpP	5	AAGAGCGGGGGATTTTGTG	ACTTTTATGGACGCAAGCGA	19	20	52.6	45.0	58.0	58.2	0.0	22.3	1.235242	70629	71117	659-666	1.000000	72.8
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	ATAGACGTCGTTTTGCCCTG	20	20	50.0	50.0	58.0	58.3	7.6	10.5	0.287189	87420	87749	347-498	1.000000	84.1
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	AGTTGGGCGCTTTAACCATT	ATAGACGTCGTTTTGCCCTG	20	20	45.0	50.0	58.4	58.3	7.6	10.5	0.650125	87420	87749	348-499	1.000000	81.1
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	TTGGGCGCTTTAACCATTCA	ATAGACGTCGTTTTGCCCTG	20	20	45.0	50.0	58.4	58.3	7.6	10.5	0.655996	87420	87749	346-497	1.000000	81.1
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	CCAATTATGAGTTGGGCGCT	ATAGACGTCGTTTTGCCCTG	20	20	50.0	50.0	58.6	58.3	7.6	10.5	0.885399	87420	87749	357-508	1.000000	79.2
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	GAGTTGGGCGCTTTAACCAT	ATAGACGTCGTTTTGCCCTG	20	20	50.0	50.0	58.8	58.3	7.6	10.5	1.104076	87420	87749	349-500	1.000000	77.5
ycf1_p1	ycf1	1	CTTCATAGTGGCTCCGTTCC	TTCCCAGTACCCCCATGAAT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.064276	109342	110676	2619-2700	1.000000	80.8
ycf1_p2	ycf1	2	TCGTAACTCCCGGAACTTCT	TTCCCAGTACCCCCATGAAT	20	20	50.0	50.0	58.1	58.0	0.7	0.0	0.090460	109342	110676	2637-2718	1.000000	80.6
ycf1_p3	ycf1	3	CAATTCCCGTTCTCAACCCA	TTCCCAGTACCCCCATGAAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.101314	109342	110676	2683-2764	1.000000	80.5
ycf1_p4	ycf1	4	CTTCATAGTGGCTCCGTTCC	ACTGCAGTCGATCTTCAACC	20	20	55.0	50.0	58.1	57.9	0.0	15.2	0.144729	109342	110676	1938-2008	1.000000	80.4
ycf1_p5	ycf1	5	ATTCCCGTTCTCAACCCATG	TTCCCAGTACCCCCATGAAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.134372	109342	110676	2681-2762	1.000000	80.2
ycf1-ndhF_p1	ycf1-ndhF	1	GCATCCACGTACAATCCACA	TTCCCAGTACCCCCATGAAT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.272661	110677	110783	875-889	1.000000	79.7
ycf1-ndhF_p2	ycf1-ndhF	2	CGAAATTACTCTGCGGTGGA	TTCCCAGTACCCCCATGAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.010184	110677	110783	823-823	0.333333	53.3
ycf1-ndhF_p3	ycf1-ndhF	3	CGAAATTACTCTGCGGTGGA	TTTCCCAGTACCCCCATGAA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.242872	110677	110783	824-824	0.333333	51.5
ycf1-ndhF_p4	ycf1-ndhF	4	CGAAATTACTCTGCGGTGGA	AAATATATAGGGCGCGGACG	20	20	50.0	50.0	58.0	57.7	0.0	8.8	0.293543	110677	110783	288-288	0.333333	51.3
ycf1-ndhF_p5	ycf1-ndhF	5	CGAAATTACTCTGCGGTGGA	AAAAGCTTTCCCAGTACCCC	20	20	50.0	50.0	58.0	57.7	0.0	13.1	0.308804	110677	110783	830-830	0.333333	51.0
ccsA_p1	ccsA	1	TGGCTCTTTCAATCTCGACG	CGGTTTTGTTGCGGAATTGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.130622	114775	115740	1760-1761	1.000000	79.9
ccsA_p2	ccsA	2	GTGAAATCGGTAGACACGCT	CGGTTTTGTTGCGGAATTGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.134744	114775	115740	1696-1697	1.000000	79.9
ccsA_p3	ccsA	3	TGAAATCGGTAGACACGCTG	CGGTTTTGTTGCGGAATTGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.136114	114775	115740	1695-1696	1.000000	79.9
ccsA_p4	ccsA	4	TGGCTCTTTCAATCTCGACG	CTTCTCTTGCATTGCCAGGA	20	20	50.0	50.0	58.0	58.2	0.0	9.6	0.173429	114775	115740	1785-1786	1.000000	79.6
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	CTTCTCTTGCATTGCCAGGA	20	20	50.0	50.0	58.0	58.2	0.0	9.6	0.177551	114775	115740	1721-1722	1.000000	79.6
ccsA-ndhD_p1	ccsA-ndhD	1	TGCAATTGTAGCTTCTATGGGA	TGCACTATCAGTTGACAAGGT	22	21	40.9	42.9	57.5	57.2	7.4	0.0	4.271844	115741	115976	403-404	1.000000	49.6
ccsA-ndhD_p2	ccsA-ndhD	2	TGCAATTGTAGCTTCTATGGGA	TCCGGATTTTGTTTTTGCACT	22	21	40.9	38.1	57.5	57.2	7.4	0.0	4.312416	115741	115976	418-419	1.000000	49.1
ccsA-ndhD_p3	ccsA-ndhD	3	TGCAATTGTAGCTTCTATGGGA	CGATCTGTATCCTTCTACCCG	22	21	40.9	52.4	57.5	57.2	7.4	0.0	4.335651	115741	115976	458-459	1.000000	48.6
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAATTGTAGCTTCTATGGGA	TTGCACTATCAGTTGACAAGGT	22	22	40.9	40.9	57.5	57.9	7.4	0.0	4.587463	115741	115976	404-405	1.000000	47.1
ccsA-ndhD_p5	ccsA-ndhD	5	CTGCAATTGTAGCTTCTATGGG	TGCACTATCAGTTGACAAGGT	22	21	45.5	42.9	57.2	57.2	10.7	0.0	4.595284	115741	115976	404-405	1.000000	47.0
ndhG-ndhI_p1	ndhG-ndhI	1	AATCATGTATTGGCGCAGGT	TCAAATTGCTTTGGGTCGGT	20	20	45.0	45.0	57.9	58.3	10.8	0.0	0.358631	119236	119707	590-605	1.000000	79.3
ndhG-ndhI_p2	ndhG-ndhI	2	ATTGGCGCAGGTAAATCCAT	TCAAATTGCTTTGGGTCGGT	20	20	45.0	45.0	57.9	58.3	10.8	0.0	0.373272	119236	119707	582-597	1.000000	79.2
ndhG-ndhI_p3	ndhG-ndhI	3	CGCCGACTATAAGACCCAAC	TCAAATTGCTTTGGGTCGGT	20	20	55.0	45.0	58.2	58.3	0.0	0.0	0.452398	119236	119707	629-644	1.000000	78.4
ndhG-ndhI_p4	ndhG-ndhI	4	ACTCCTAAACCGCCGACTAT	TCAAATTGCTTTGGGTCGGT	20	20	50.0	45.0	58.2	58.3	0.0	0.0	0.528658	119236	119707	639-654	1.000000	77.8
ndhG-ndhI_p5	ndhG-ndhI	5	GCCGACTATAAGACCCAACC	TCAAATTGCTTTGGGTCGGT	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.537339	119236	119707	628-643	1.000000	77.7
ycf15-trnI-CAU_p1	ycf15-trnI-CAU	1	GATTGCTTGTTGAACCCTGC	TGGGGATTAGCATATATTTCAAGT	20	24	50.0	33.3	57.9	55.7	0.0	0.0	6.385665	151656	151775	512-663	1.000000	45.4
ycf15-trnI-CAU_p2	ycf15-trnI-CAU	2	TAGGAAGAAGCCCCCTCAAA	TGGGGATTAGCATATATTTCAAGT	20	24	50.0	33.3	57.9	55.7	0.0	0.0	6.369151	151656	151775	626-777	1.000000	45.2
ycf15-trnI-CAU_p3	ycf15-trnI-CAU	3	GGTATAGGAAGAAGCCCCCT	TGGGGATTAGCATATATTTCAAGT	20	24	55.0	33.3	57.9	55.7	0.0	0.0	6.435343	151656	151775	630-781	1.000000	45.2
ycf15-trnI-CAU_p4	ycf15-trnI-CAU	4	TCTCAGTAGGAGTCGAACCC	TGGGGATTAGCATATATTTCAAGT	20	24	55.0	33.3	57.9	55.7	6.3	0.0	6.441177	151656	151775	908-1059	1.000000	44.8
ycf15-trnI-CAU_p5	ycf15-trnI-CAU	5	CAGTAGGAGTCGAACCCAGA	TGGGGATTAGCATATATTTCAAGT	20	24	55.0	33.3	58.2	55.7	7.0	0.0	6.479909	151656	151775	905-1056	1.000000	44.8
psbB_p1	psbB	1	CCACACCTGTTCATTTGGGA	TTATTTGATAAAGGGGTATTTTC	20	23	50.0	26.1	58.0	50.5	0.0	0.0	10.459574	164129	165655	1706-1706	0.833333	33.4
psbB_p2	psbB	2	TCCACACCTGTTCATTTGGG	TTATTTGATAAAGGGGTATTTTC	20	23	50.0	26.1	58.0	50.5	0.0	0.0	10.459574	164129	165655	1707-1707	0.833333	33.4
psbB_p3	psbB	3	CCTTAGGCGGTTCTCGAAAA	TTATTTGATAAAGGGGTATTTTC	20	23	50.0	26.1	57.9	50.5	0.0	0.0	10.542480	164129	165655	1821-1821	0.833333	33.4
psbB_p4	psbB	4	CCACACCTGTTCATTTGGGA	TTTATTTGATAAAGGGGTATTTTC	20	24	50.0	25.0	58.0	51.5	0.0	0.0	10.545406	164129	165655	1707-1707	0.833333	33.4
psbB_p5	psbB	5	TCCACACCTGTTCATTTGGG	TTTATTTGATAAAGGGGTATTTTC	20	24	50.0	25.0	58.0	51.5	0.0	0.0	10.545406	164129	165655	1708-1708	0.833333	33.4