primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	76	303	292-295	1.000000	83.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	76	303	295-298	1.000000	83.2
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	76	303	306-309	1.000000	83.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	76	303	305-308	1.000000	83.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	76	303	307-310	1.000000	83.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAAATCCACGAACGATCCCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	0.1	16.9	0.710402	5752	6918	1266-1271	1.000000	76.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5752	6918	1232-1237	1.000000	74.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	59.1	58.1	0.0	16.9	1.134559	5752	6918	1235-1240	1.000000	72.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAATCCACGAACGATCCCG	GAGGTTCGAATCCTTCCGTC	19	20	52.6	55.0	57.9	58.1	0.1	16.9	1.135704	5752	6918	1265-1270	1.000000	72.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAAATCCACGAACGATCCCG	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	58.6	57.3	0.1	16.9	1.301774	5752	6918	1270-1275	1.000000	71.3
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	ACGGAAAGAGAGGGATTCGA	AGAACTTGCGTCCAATAGGA	20	20	50.0	45.0	58.2	56.6	0.0	0.0	1.594719	8183	10020	1986-2006	1.000000	69.5
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	TAGCAATCCGACGCTTTAGG	AGAACTTGCGTCCAATAGGA	20	20	50.0	45.0	57.8	56.6	0.0	0.0	1.662324	8183	10020	1935-1955	1.000000	69.0
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	ACGGAAAGAGAGGGATTCGA	ACATAGAAGAACTTGCGTCCA	20	21	50.0	42.9	58.2	57.3	0.0	0.0	1.829404	8183	10020	1993-2013	1.000000	67.6
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	ACGGAAAGAGAGGGATTCGA	AAGAACTTGCGTCCAATAGGA	20	21	50.0	42.9	58.2	57.3	0.0	0.0	1.847178	8183	10020	1987-2007	1.000000	67.5
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	TAGCAATCCGACGCTTTAGG	ACATAGAAGAACTTGCGTCCA	20	21	50.0	42.9	57.8	57.3	0.0	0.0	1.897009	8183	10020	1942-1962	1.000000	67.1
atpF-atpH_p1	atpF-atpH	1	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.554161	13426	14064	724-729	1.000000	78.5
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTCGCTTTAGC	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.662793	13426	14064	756-761	1.000000	77.5
atpF-atpH_p3	atpF-atpH	3	GGCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	20	20	50.0	50.0	58.0	57.3	0.0	0.0	0.695531	13426	14064	789-794	1.000000	77.2
atpF-atpH_p4	atpF-atpH	4	GGCCCAAGGAAAGGAAAGAA	GCGGAGGGAAAAATACGAGG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.712954	13426	14064	790-795	1.000000	77.0
atpF-atpH_p5	atpF-atpH	5	GGCCCAAGGAAAGGAAAGAA	GGCGGAGGGAAAAATACGAG	20	20	50.0	55.0	58.0	58.7	0.0	0.0	0.712954	13426	14064	791-796	1.000000	77.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCACTTCCATCCACCAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	59.0	58.4	0.0	14.6	1.361244	27517	28961	1509-1510	1.000000	70.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCACTTCCATCCACC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	59.0	58.4	0.0	14.6	1.363571	27517	28961	1514-1515	1.000000	70.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GACATTCCCTCACTTCCATCC	AAAAGGATTTGCAGTCCCCC	21	20	52.4	50.0	58.1	58.4	0.0	14.6	1.431069	27517	28961	1518-1519	1.000000	69.5
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CATTCCCTCACTTCCATCCA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	56.9	58.4	0.0	14.6	1.463812	27517	28961	1516-1517	1.000000	69.2
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ATTCCCTCACTTCCATCCAC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	56.9	58.4	0.0	14.6	1.465977	27517	28961	1515-1516	1.000000	69.2
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	31876	32855	1121-1126	1.000000	79.3
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	31876	32855	1100-1105	1.000000	78.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	31876	32855	1097-1102	1.000000	78.7
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	31876	32855	1062-1067	1.000000	78.4
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	31876	32855	1118-1123	1.000000	78.2
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.1	57.8	0.0	1.1	0.296135	37572	38096	671-677	1.000000	78.8
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	57.7	57.8	0.0	1.1	0.505148	37572	38096	670-676	1.000000	77.1
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	CGTTGTGTTTGCTTCTCCTG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.6	57.8	0.0	1.1	0.559545	37572	38096	690-696	1.000000	76.7
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	TGTGTTTGCTTCTCCTGACG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.587899	37572	38096	687-693	1.000000	76.5
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	CCGTTGTGTTTGCTTCTCCT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.7	57.8	0.0	1.1	0.860834	37572	38096	691-697	1.000000	74.3
ycf3_p1	ycf3	1	CATTTGACTAATCAGACTAATAAATCA	TCCTTTGTTTGTGGGGAATCA	27	21	25.9	42.9	54.2	57.7	0.0	0.0	12.103219	44057	46131	2175-2184	1.000000	41.8
ycf3_p2	ycf3	2	CATTTGACTAATCAGACTAATAAATC	TCCTTTGTTTGTGGGGAATCA	26	21	26.9	42.9	52.7	57.7	0.0	0.0	12.614818	44057	46131	2175-2184	1.000000	41.8
ycf3_p3	ycf3	3	CATTTGACTAATCAGACTAATAAATCA	ACAATTCCTTTGTTTGTGGGG	27	21	25.9	42.9	54.2	57.2	0.0	0.0	12.618973	44057	46131	2180-2189	1.000000	41.8
ycf3_p4	ycf3	4	CATTTGACTAATCAGACTAATAAATCA	CCTTTGTTTGTGGGGAATCA	27	20	25.9	45.0	54.2	56.1	0.0	0.0	12.665965	44057	46131	2174-2183	1.000000	41.8
ycf3_p5	ycf3	5	CATTTGACTAATCAGACTAATAAATCA	CAATTCCTTTGTTTGTGGGGA	27	21	25.9	42.9	54.2	56.9	0.0	0.0	12.896637	44057	46131	2179-2188	1.000000	41.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48033	48830	848-850	1.000000	80.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48033	48830	856-858	1.000000	79.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	TAGACAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	33.1	0.0	0.223577	48033	48830	899-901	1.000000	79.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48033	48830	880-882	1.000000	79.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TAGACAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	33.1	0.0	0.288386	48033	48830	907-909	1.000000	78.9
trnL-UAA_p1	trnL-UAA	1	ACACTGCTTTGATTTCAGCA	AGGATAAAGCATTAACGAGTCA	20	22	40.0	36.4	56.2	55.3	0.0	0.0	6.562856	48831	49450	675-685	1.000000	41.4
trnL-UAA_p2	trnL-UAA	2	ACACTGCTTTGATTTCAGCA	AGCATTAACGAGTCAAATGT	20	20	40.0	35.0	56.2	53.2	0.0	10.1	6.618595	48831	49450	668-678	1.000000	41.4
trnL-UAA_p3	trnL-UAA	3	ACACTGCTTTGATTTCAGCA	AAGCATTAACGAGTCAAATGT	20	21	40.0	33.3	56.2	54.1	0.0	10.1	6.721940	48831	49450	669-679	1.000000	41.4
trnL-UAA_p4	trnL-UAA	4	ACACTGCTTTGATTTCAGCA	AGCATTAACGAGTCAAATGTT	20	21	40.0	33.3	56.2	54.1	0.0	10.1	6.721940	48831	49450	668-678	1.000000	41.4
trnL-UAA_p5	trnL-UAA	5	ACACTGCTTTGATTTCAGCA	AGGATAAAGCATTAACGAGTCAA	20	23	40.0	34.8	56.2	56.0	0.0	0.0	6.824138	48831	49450	675-685	1.000000	41.4
trnC-GCA-ndhJ_p1	trnC-GCA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ACTCATCCACGACTGAAACG	20	20	55.0	50.0	58.0	57.9	10.3	0.0	0.068498	49797	50642	1010-1024	1.000000	81.0
trnC-GCA-ndhJ_p2	trnC-GCA-ndhJ	2	GGGATAGCTCAGCTGGTAGA	CTCATCCACGACTGAAACGT	20	20	55.0	50.0	58.0	57.9	10.3	0.0	0.068498	49797	50642	1009-1023	1.000000	81.0
trnC-GCA-ndhJ_p3	trnC-GCA-ndhJ	3	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	49797	50642	974-988	1.000000	80.9
trnC-GCA-ndhJ_p4	trnC-GCA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ACTCATCCACGACTGAAACG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.117888	49797	50642	974-988	1.000000	80.7
trnC-GCA-ndhJ_p5	trnC-GCA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	CTCATCCACGACTGAAACGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.117888	49797	50642	973-987	1.000000	80.7
rbcL-accD_p1	rbcL-accD	1	GCTGCTTGTGAGGTATGGAA	TTTCCCGCTAGACAACATCG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.118443	58181	58835	755-757	1.000000	80.1
rbcL-accD_p2	rbcL-accD	2	GAGTCCTGAACTAGCTGCTG	TTTCCCGCTAGACAACATCG	20	20	55.0	50.0	57.7	58.0	13.4	0.0	0.296685	58181	58835	771-773	1.000000	78.7
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	CCCGCTAGACAACATCGAAT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.338929	58181	58835	752-754	1.000000	78.4
rbcL-accD_p4	rbcL-accD	4	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.384577	58181	58835	741-743	1.000000	78.0
rbcL-accD_p5	rbcL-accD	5	GCTGCTTGTGAGGTATGGAA	CGAATTAAACCGCCGTTGTT	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.433102	58181	58835	737-739	1.000000	77.6
petL-petG_p1	petL-petG	1	TCATTTAGCCCCTTCATGCT	GGTCTAACTGATCACCACGT	20	20	45.0	50.0	56.9	57.0	0.0	0.0	2.162320	67281	67473	413-413	1.000000	63.9
petL-petG_p2	petL-petG	2	TCATTTAGCCCCTTCATGCT	GGTCTAACTGATCACCACGTC	20	21	45.0	52.4	56.9	58.1	0.0	0.0	2.252771	67281	67473	413-413	1.000000	63.1
petL-petG_p3	petL-petG	3	TCATTTAGCCCCTTCATGCTT	GGTCTAACTGATCACCACGT	21	20	42.9	50.0	57.6	57.0	0.0	0.0	2.419146	67281	67473	413-413	1.000000	61.8
petL-petG_p4	petL-petG	4	TTCATTTAGCCCCTTCATGCT	GGTCTAACTGATCACCACGT	21	20	42.9	50.0	57.6	57.0	0.0	0.0	2.419146	67281	67473	414-414	1.000000	61.8
petL-petG_p5	petL-petG	5	TCATTTAGCCCCTTCATGCT	AGGTCTAACTGATCACCACG	20	20	45.0	50.0	56.9	56.7	0.0	0.0	2.458290	67281	67473	414-414	1.000000	61.5
petG-trnW-CCA_p1	petG-trnW-CCA	1	ACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	57.0	57.9	0.0	1.4	1.121164	67588	67724	201-201	1.000000	79.4
petG-trnW-CCA_p2	petG-trnW-CCA	2	GACGTGGTGATCAGTTAGACC	CAAAACCCGATGTCGTAGGT	21	20	52.4	50.0	58.1	57.9	0.0	1.4	1.211615	67588	67724	202-202	1.000000	78.6
petG-trnW-CCA_p3	petG-trnW-CCA	3	ACGTGGTGATCAGTTAGACC	CCCGATGTCGTAGGTTCAAA	20	20	50.0	50.0	57.0	57.6	0.0	1.4	1.405755	67588	67724	196-196	1.000000	77.3
petG-trnW-CCA_p4	petG-trnW-CCA	4	ACGTGGTGATCAGTTAGACC	CTCCAAAACCCGATGTCGTA	20	20	50.0	50.0	57.0	57.6	0.0	1.4	1.405755	67588	67724	204-204	1.000000	77.0
petG-trnW-CCA_p5	petG-trnW-CCA	5	ACGTGGTGATCAGTTAGACC	TCCAAAACCCGATGTCGTAG	20	20	50.0	50.0	57.0	57.6	0.0	1.4	1.405755	67588	67724	203-203	1.000000	77.0
ndhF_p1	ndhF	1	TCCAAAATGTCAATCCAGAGT	TCGAAGGAGTTGAAAAAGAA	21	20	38.1	35.0	55.1	52.7	0.0	0.0	9.218689	112682	114910	2306-2312	1.000000	41.2
ndhF_p2	ndhF	2	CATCCAAAATGTCAATCCAGAGT	TCGAAGGAGTTGAAAAAGAA	23	20	39.1	35.0	57.1	52.7	0.0	0.0	9.230881	112682	114910	2308-2314	1.000000	41.2
ndhF_p3	ndhF	3	TCCAACAAGGAAGAATTGTCT	TCGAAGGAGTTGAAAAAGAA	21	20	38.1	35.0	55.0	52.7	0.0	0.0	9.287480	112682	114910	2363-2369	1.000000	41.2
ndhF_p4	ndhF	4	TCATCCAAAATGTCAATCCAGAG	TCGAAGGAGTTGAAAAAGAA	23	20	39.1	35.0	56.8	52.7	0.0	0.0	9.484480	112682	114910	2309-2315	1.000000	41.2
ndhF_p5	ndhF	5	TCCAACAAGGAAGAATTGTCTT	TCGAAGGAGTTGAAAAAGAA	22	20	36.4	35.0	55.8	52.7	0.0	0.0	9.495815	112682	114910	2363-2369	1.000000	41.2
ndhD-psaC_p1	ndhD-psaC	1	AGAAAATTAAAGAACCCGCCA	ATGGCATGAAACCACTCGAA	21	20	38.1	45.0	56.0	57.8	0.0	0.0	3.152384	119143	119282	247-249	1.000000	56.7
ndhD-psaC_p2	ndhD-psaC	2	AGAAAATTAAAGAACCCGCCA	CACTCGAAGCATGGGTCTAG	21	20	38.1	55.0	56.0	57.8	0.0	0.0	3.169464	119143	119282	235-237	1.000000	56.7
ndhD-psaC_p3	ndhD-psaC	3	AGAAAATTAAAGAACCCGCCA	AGAGATGTGAATCCGCTTGC	21	20	38.1	50.0	56.0	58.3	0.0	0.0	3.292215	119143	119282	299-301	1.000000	55.3
ndhD-psaC_p4	ndhD-psaC	4	AAGAAAATTAAAGAACCCGCCA	ATGGCATGAAACCACTCGAA	22	20	36.4	45.0	56.8	57.8	0.0	0.0	3.412941	119143	119282	248-250	1.000000	54.6
ndhD-psaC_p5	ndhD-psaC	5	AGAAAATTAAAGAACCCGCCAA	ATGGCATGAAACCACTCGAA	22	20	36.4	45.0	56.8	57.8	0.0	0.0	3.412941	119143	119282	247-249	1.000000	54.6
trnI-CAU-rpl23_p1	trnI-CAU-rpl23	1	GAATGGTTAAAGCGCCCAAC	TCGACCCAATGCTTTACTTCT	20	21	50.0	42.9	58.0	57.3	2.3	0.0	1.705031	154252	154434	350-364	1.000000	69.9
trnI-CAU-rpl23_p2	trnI-CAU-rpl23	2	GAATGGTTAAAGCGCCCAAC	CGACCCAATGCTTTACTTCTG	20	21	50.0	47.6	58.0	57.3	2.3	0.0	1.756300	154252	154434	349-363	1.000000	69.5
trnI-CAU-rpl23_p3	trnI-CAU-rpl23	3	GAATGGTTAAAGCGCCCAAC	TTCGACCCAATGCTTTACTTCT	20	22	50.0	40.9	58.0	58.0	2.3	0.0	2.025073	154252	154434	351-365	1.000000	67.4
trnI-CAU-rpl23_p4	trnI-CAU-rpl23	4	GCTAAGCATCCATGGCTGAA	TCGACCCAATGCTTTACTTCT	20	21	50.0	42.9	58.3	57.3	19.8	0.0	2.011887	154252	154434	367-381	1.000000	67.3
trnI-CAU-rpl23_p5	trnI-CAU-rpl23	5	GCTAAGCATCCATGGCTGAA	CGACCCAATGCTTTACTTCTG	20	21	50.0	47.6	58.3	57.3	19.8	0.0	2.063156	154252	154434	366-380	1.000000	66.9