primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
chlI-trnF_p1	chlI-trnF	1	TGCTTACGTCACCGTCTAAG	GAACTGGTGACACGAGGATC	20	20	50.0	55.0	57.4	58.0	0.0	20.1	0.626647	2830	2944	267-267	1.000000	75.9
chlI-trnF_p2	chlI-trnF	2	TGCTTACGTCACCGTCTAAG	TCCACTGCTCTACCAACTGA	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.633998	2830	2944	242-242	1.000000	75.9
chlI-trnF_p3	chlI-trnF	3	TGCTTACGTCACCGTCTAAG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.4	58.1	0.0	20.1	0.676212	2830	2944	270-270	1.000000	75.5
chlI-trnF_p4	chlI-trnF	4	TGCTTACGTCACCGTCTAAG	CTGGTGACACGAGGATCTTC	20	20	50.0	55.0	57.4	57.7	0.0	9.6	0.910143	2830	2944	264-264	1.000000	73.6
chlI-trnF_p5	chlI-trnF	5	GCTTACGTCACCGTCTAAGA	GAACTGGTGACACGAGGATC	20	20	50.0	55.0	57.1	58.0	0.0	20.1	0.910241	2830	2944	266-266	1.000000	73.6
tsf_p1	tsf	1	GGGTTCTCACAGTTTCTCCA	AGGTCAAACGTTAATCACAACA	20	22	50.0	36.4	57.1	56.5	0.0	0.0	4.432469	9128	9730	720-720	1.000000	44.8
tsf_p2	tsf	2	GGGTTCTCACAGTTTCTCCAG	AGGTCAAACGTTAATCACAACA	21	22	52.4	36.4	58.2	56.5	0.0	0.0	4.729355	9128	9730	720-720	1.000000	42.4
tsf_p3	tsf	3	AGGGTTCTCACAGTTTCTCC	AGGTCAAACGTTAATCACAACA	20	22	50.0	36.4	56.8	56.5	0.0	0.0	4.734354	9128	9730	721-721	1.000000	42.3
tsf_p4	tsf	4	GGGTTCTCACAGTTTCTCCA	AAGGTCAAACGTTAATCACAACA	20	23	50.0	34.8	57.1	57.2	0.0	0.0	4.752343	9128	9730	721-721	1.000000	42.2
tsf_p5	tsf	5	GGGTTCTCACAGTTTCTCCA	GGTCAAACGTTAATCACAACA	20	21	50.0	38.1	57.1	55.0	0.0	0.0	4.911805	9128	9730	719-719	1.000000	40.9
rpoC2_p1	rpoC2	1	TCTGTATCATTTGATTGAAGTGA	TCGCCCTATAACTCGGTATGA	23	21	30.4	47.6	53.8	57.9	0.0	0.0	8.373434	12109	12228	201-201	1.000000	41.2
rpoC2_p2	rpoC2	2	TCTGTATCATTTGATTGAAGTGAA	TCGCCCTATAACTCGGTATGA	24	21	29.2	47.6	54.5	57.9	0.0	0.0	8.598474	12109	12228	201-201	1.000000	41.2
rpoC2_p3	rpoC2	3	TTCTGTATCATTTGATTGAAGTGA	TCGCCCTATAACTCGGTATGA	24	21	29.2	47.6	54.5	57.9	0.0	0.0	8.598474	12109	12228	202-202	1.000000	41.2
rpoC2_p4	rpoC2	4	TTCTGTATCATTTGATTGAAGTGAA	TCGCCCTATAACTCGGTATGA	25	21	28.0	47.6	55.3	57.9	0.0	0.0	8.885587	12109	12228	202-202	1.000000	41.2
rpoC2_p5	rpoC2	5	TCTGTATCATTTGATTGAAGTGA	CGCCCTATAACTCGGTATGA	23	20	30.4	50.0	53.8	56.3	0.0	0.0	8.905723	12109	12228	200-200	1.000000	41.2
trnP-chlB_p1	trnP-chlB	1	CCAAAGCAGATACGCTACCA	CGAAACACCGAAAAGTTTGCT	20	21	50.0	42.9	57.7	58.2	0.0	0.0	1.516779	21203	21275	197-197	1.000000	68.3
trnP-chlB_p2	trnP-chlB	2	CCAAAGCAGATACGCTACCA	CGAAACACCGAAAAGTTTGC	20	20	50.0	45.0	57.7	56.8	0.0	0.0	1.556097	21203	21275	197-197	1.000000	68.0
trnP-chlB_p3	trnP-chlB	3	CCCAAAGCAGATACGCTACC	CGAAACACCGAAAAGTTTGCT	20	21	55.0	42.9	58.4	58.2	0.0	0.0	1.624197	21203	21275	198-198	1.000000	67.4
trnP-chlB_p4	trnP-chlB	4	CCCAAAGCAGATACGCTACC	CGAAACACCGAAAAGTTTGC	20	20	55.0	45.0	58.4	56.8	0.0	0.0	1.663516	21203	21275	198-198	1.000000	67.1
trnP-chlB_p5	trnP-chlB	5	CCAAAGCAGATACGCTACCA	ACACCGAAAAGTTTGCTCAAG	20	21	50.0	42.9	57.7	57.6	0.0	0.0	1.730367	21203	21275	193-193	1.000000	66.6
chlB_p1	chlB	1	ACTAGGTTATTTATGTCAAGAAGA	AGAGGGTGTTTCTATTGAACACT	24	23	29.2	39.1	53.2	57.4	0.0	16.7	12.376182	21276	22808	1629-1629	1.000000	40.4
chlB_p2	chlB	2	ACTAGGTTATTTATGTCAAGAAGA	AGGGTGTTTCTATTGAACACTCT	24	23	29.2	39.1	53.2	57.4	0.0	14.8	12.376182	21276	22808	1627-1627	1.000000	40.4
chlB_p3	chlB	3	ACTAGGTTATTTATGTCAAGAAGA	GAGGGTGTTTCTATTGAACACTC	24	23	29.2	43.5	53.2	57.1	0.0	16.7	12.672191	21276	22808	1628-1628	1.000000	40.4
chlB_p4	chlB	4	ACTAGGTTATTTATGTCAAGAAGA	AGGGTGTTTCTATTGAACACTC	24	22	29.2	40.9	53.2	56.0	0.0	16.7	12.795407	21276	22808	1627-1627	1.000000	40.4
chlB_p5	chlB	5	ACTAGGTTATTTATGTCAAGAAGA	GGGTGTTTCTATTGAACACTCT	24	22	29.2	40.9	53.2	56.0	0.0	16.7	12.795407	21276	22808	1626-1626	1.000000	40.4
trnY-ORF1_p1	trnY-ORF1	1	AAGGGGGCGGATTGTAAATC	ACTTAGGTCGAGATGGTCCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.289425	23102	23144	183-183	1.000000	78.1
trnY-ORF1_p2	trnY-ORF1	2	AAGGGGGCGGATTGTAAATC	GTCGAGATGGTCCCGATTTT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.378490	23102	23144	177-177	1.000000	77.4
trnY-ORF1_p3	trnY-ORF1	3	AATCCGCTGGTTTTCCTACG	ACTTAGGTCGAGATGGTCCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.487897	23102	23144	167-167	1.000000	76.6
trnY-ORF1_p4	trnY-ORF1	4	AATCCGCTGGTTTTCCTACG	GTCGAGATGGTCCCGATTTT	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.576961	23102	23144	161-161	1.000000	75.9
trnY-ORF1_p5	trnY-ORF1	5	GCTGGTTTTCCTACGTTGGT	ACTTAGGTCGAGATGGTCCC	20	20	50.0	55.0	58.4	58.2	15.7	0.0	0.620893	23102	23144	162-162	1.000000	75.5
ycf46_p1	ycf46	1	GCAATAGCTAACAATATTATTATAGCT	GGTTGCAAAGTAATAAACAACCG	27	23	25.9	39.1	54.2	57.2	24.6	0.0	14.601469	25302	26777	1702-1702	1.000000	40.1
ycf46_p2	ycf46	2	GCAATAGCTAACAATATTATTATAGC	GGTTGCAAAGTAATAAACAACCG	26	23	26.9	39.1	52.9	57.2	17.5	0.0	14.871774	25302	26777	1702-1702	1.000000	40.1
ycf46_p3	ycf46	3	GCAATAGCTAACAATATTATTATAGCT	AAACAACCGTAGACGAAAAA	27	20	25.9	35.0	54.2	53.7	24.6	3.1	15.069881	25302	26777	1688-1688	1.000000	40.1
ycf46_p4	ycf46	4	GCAATAGCTAACAATATTATTATAGCT	GTTGCAAAGTAATAAACAACCGT	27	23	25.9	34.8	54.2	56.5	24.6	0.0	15.242594	25302	26777	1701-1701	1.000000	40.1
ycf46_p5	ycf46	5	GCAATAGCTAACAATATTATTATAGC	AAACAACCGTAGACGAAAAA	26	20	26.9	35.0	52.9	53.7	17.5	3.1	15.340186	25302	26777	1688-1688	1.000000	40.1
psbX-rpl27_p1	psbX-rpl27	1	ACAATAAGCCCACCAGCAAT	ACCAGGTGATAATGTAGGGGT	20	21	45.0	47.6	57.8	57.9	0.0	0.0	1.382048	37632	38061	600-604	1.000000	70.3
psbX-rpl27_p2	psbX-rpl27	2	CTACAATAAGCCCACCAGCA	ACCAGGTGATAATGTAGGGGT	20	21	50.0	47.6	57.6	57.9	0.0	0.0	1.563927	37632	38061	602-606	1.000000	68.8
psbX-rpl27_p3	psbX-rpl27	3	ACTACAATAAGCCCACCAGC	ACCAGGTGATAATGTAGGGGT	20	21	50.0	47.6	57.6	57.9	0.0	0.0	1.565617	37632	38061	603-607	1.000000	68.8
psbX-rpl27_p4	psbX-rpl27	4	ACAATAAGCCCACCAGCAAT	TGCATTTCAGTATCTGCCACT	20	21	45.0	42.9	57.8	57.6	0.0	0.0	1.591437	37632	38061	509-513	1.000000	68.8
psbX-rpl27_p5	psbX-rpl27	5	CTACAATAAGCCCACCAGCA	TGCATTTCAGTATCTGCCACT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.773316	37632	38061	511-515	1.000000	67.4
ycf42_p1	ycf42	1	AAGTCCATTACGGTCATGCC	GCTACCCGGAACAAACTTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.7	0.151500	41912	42472	1250-1250	1.000000	79.0
ycf42_p2	ycf42	2	GTCCATTACGGTCATGCCTT	GCTACCCGGAACAAACTTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.7	0.151500	41912	42472	1248-1248	1.000000	79.0
ycf42_p3	ycf42	3	TTTTTCCAACGAGCAGGGAA	GCTACCCGGAACAAACTTGA	20	20	45.0	50.0	57.9	58.1	0.0	0.7	0.173164	41912	42472	1212-1212	1.000000	78.8
ycf42_p4	ycf42	4	TGAGAGTTGGTGCCAAGATG	GCTACCCGGAACAAACTTGA	20	20	50.0	50.0	57.8	58.1	3.6	0.7	0.302707	41912	42472	1594-1594	1.000000	77.7
ycf42_p5	ycf42	5	GTCCATTACGGTCATGCCTT	TTGCTACCCGGAACAAACTT	20	20	50.0	45.0	58.0	57.6	0.0	0.7	0.390093	41912	42472	1250-1250	1.000000	77.1
trnE-psaA_p1	trnE-psaA	1	TAAGCCCCCATCGTCTAGAG	ACGTGAAAAATGACCTGGCT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.021945	47795	48028	412-412	1.000000	80.8
trnE-psaA_p2	trnE-psaA	2	CATCTCCTTTTCACGGAGGG	ACGTGAAAAATGACCTGGCT	20	20	55.0	45.0	58.0	58.0	6.3	0.0	0.048582	47795	48028	384-384	1.000000	80.7
trnE-psaA_p3	trnE-psaA	3	ATCTCCTTTTCACGGAGGGA	ACGTGAAAAATGACCTGGCT	20	20	50.0	45.0	58.0	58.0	6.3	0.0	0.066122	47795	48028	383-383	1.000000	80.5
trnE-psaA_p4	trnE-psaA	4	TAAGCCCCCATCGTCTAGAG	TTGGCTGCTTGCTTATTGGA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.081219	47795	48028	336-336	1.000000	80.5
trnE-psaA_p5	trnE-psaA	5	CATCTCCTTTTCACGGAGGG	TTGGCTGCTTGCTTATTGGA	20	20	55.0	45.0	58.0	58.1	6.3	0.0	0.107856	47795	48028	308-308	1.000000	80.4
chlL_p1	chlL	1	ACCAATCAATATCTCTTAAGAGCGA	TCCCTGTTTCACATTCAAAA	25	20	36.0	35.0	57.9	53.2	25.0	0.0	9.875744	55097	55981	1100-1100	1.000000	40.3
chlL_p2	chlL	2	ACCAATCAATATCTCTTAAGAGCG	TCCCTGTTTCACATTCAAAA	24	20	37.5	35.0	56.7	53.2	25.0	0.0	10.162285	55097	55981	1100-1100	1.000000	40.3
chlL_p3	chlL	3	CCAATCAATATCTCTTAAGAGCGAC	TCCCTGTTTCACATTCAAAA	25	20	40.0	35.0	57.7	53.2	25.0	0.0	10.164988	55097	55981	1099-1099	1.000000	40.3
chlL_p4	chlL	4	ACCAATCAATATCTCTTAAGAGCGA	TCCCTGTTTCACATTCAAAAT	25	21	36.0	33.3	57.9	53.9	25.0	0.0	10.194865	55097	55981	1100-1100	1.000000	40.3
chlL_p5	chlL	5	ACCAATCAATATCTCTTAAGAGCGA	TCCCTGTTTCACATTCAAAATT	25	22	36.0	31.8	57.9	54.7	25.0	0.0	10.351417	55097	55981	1100-1100	1.000000	40.3
chlN-psaF_p1	chlN-psaF	1	ACCACTTCGCCGTAATCAAA	TGCTGCTAAAGGTATGCTGA	20	20	45.0	45.0	57.8	56.9	0.0	0.0	1.251151	57391	57525	316-316	1.000000	70.5
chlN-psaF_p2	chlN-psaF	2	GACCACTTCGCCGTAATCAA	TGCTGCTAAAGGTATGCTGA	20	20	50.0	45.0	58.3	56.9	0.0	0.0	1.361106	57391	57525	317-317	1.000000	69.6
chlN-psaF_p3	chlN-psaF	3	GCGTTATACTGACCGTCGAT	TGCTGCTAAAGGTATGCTGA	20	20	50.0	45.0	57.6	56.9	0.0	0.0	1.474460	57391	57525	274-274	1.000000	68.8
chlN-psaF_p4	chlN-psaF	4	GAGACCACTTCGCCGTAATC	TGCTGCTAAAGGTATGCTGA	20	20	55.0	45.0	58.4	56.9	0.0	0.0	1.520648	57391	57525	319-319	1.000000	68.3
chlN-psaF_p5	chlN-psaF	5	CAGCGTTATACTGACCGTCG	TGCTGCTAAAGGTATGCTGA	20	20	55.0	45.0	58.5	56.9	0.0	0.0	1.614495	57391	57525	276-276	1.000000	67.7
atpB_p1	atpB	1	CTTCGTCGGCAGAAGTATCC	ACTTGTTTTACTTTTGTTCTTAAGCT	20	26	55.0	26.9	58.2	56.5	11.7	0.0	7.667705	62470	63915	1615-1615	1.000000	40.2
atpB_p2	atpB	2	GGTAGAACTACTTCGTCGGC	ACTTGTTTTACTTTTGTTCTTAAGCT	20	26	55.0	26.9	57.8	56.5	11.0	0.0	7.695021	62470	63915	1625-1625	1.000000	40.2
atpB_p3	atpB	3	CTTCGTCGGCAGAAGTATCC	AGACTTGTTTTACTTTTGTTCTTAAGC	20	27	55.0	29.6	58.2	57.4	11.7	0.0	7.727718	62470	63915	1617-1617	1.000000	40.2
atpB_p4	atpB	4	CTTCGTCGGCAGAAGTATCC	GACTTGTTTTACTTTTGTTCTTAAGCT	20	27	55.0	29.6	58.2	57.4	11.7	0.0	7.727718	62470	63915	1616-1616	1.000000	40.2
atpB_p5	atpB	5	GGTAGAACTACTTCGTCGGC	GACTTGTTTTACTTTTGTTCTTAAGCT	20	27	55.0	29.6	57.8	57.4	11.0	0.0	7.755034	62470	63915	1626-1626	1.000000	40.2
psbA-cbbX_p1	psbA-cbbX	1	CTGCTCCTTCTGTTGTTGGT	GAGCTCGAATGAGACAAGCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028940	70188	70885	837-840	1.000000	82.2
psbA-cbbX_p2	psbA-cbbX	2	TGTTGCGATTTCTGCTCCTT	GAGCTCGAATGAGACAAGCA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.105592	70188	70885	848-851	1.000000	81.5
psbA-cbbX_p3	psbA-cbbX	3	TTCCTGTTGCGATTTCTGCT	GAGCTCGAATGAGACAAGCA	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.105592	70188	70885	852-855	1.000000	81.5
psbA-cbbX_p4	psbA-cbbX	4	CTTCCTGTTGCGATTTCTGC	GAGCTCGAATGAGACAAGCA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.255204	70188	70885	853-856	1.000000	80.3
psbA-cbbX_p5	psbA-cbbX	5	CTGCTCCTTCTGTTGTTGGT	CGAATGAGACAAGCAAACCG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.259367	70188	70885	832-835	1.000000	80.3
psbJ-psbL_p1	psbJ-psbL	1	GCCACCAACTAATGCGACTA	CGTACGTCTTTATACTGGGGG	20	21	50.0	52.4	58.0	57.6	0.0	7.2	1.378791	72987	73053	193-193	1.000000	69.4
psbJ-psbL_p2	psbJ-psbL	2	CCTAGGCCACCAACTAATGC	CGTACGTCTTTATACTGGGGG	20	21	55.0	52.4	58.3	57.6	5.0	7.2	1.679912	72987	73053	198-198	1.000000	67.0
psbJ-psbL_p3	psbJ-psbL	3	AACTCCTAGGCCACCAACTA	CGTACGTCTTTATACTGGGGG	20	21	50.0	52.4	57.7	57.6	5.0	7.2	1.697608	72987	73053	202-202	1.000000	66.8
psbJ-psbL_p4	psbJ-psbL	4	ACTCCTAGGCCACCAACTAA	CGTACGTCTTTATACTGGGGG	20	21	50.0	52.4	57.7	57.6	5.0	7.2	1.697608	72987	73053	201-201	1.000000	66.8
psbJ-psbL_p5	psbJ-psbL	5	TAACTCCTAGGCCACCAACT	CGTACGTCTTTATACTGGGGG	20	21	50.0	52.4	57.7	57.6	5.0	7.2	1.697608	72987	73053	203-203	1.000000	66.8