primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	atpA-atpF	1	CCGTCGCCTACTTGAAGTAC	ATTGGCATGTTTGGGACGAT	20	20	55.0	45.0	58.1	58.1	0.0	0.0	0.243247	12875	12938	216-217	1.000000	79.2
atpA-atpF_p2	atpA-atpF	2	GTCGCCTACTTGAAGTACGG	ATTGGCATGTTTGGGACGAT	20	20	55.0	45.0	58.1	58.1	13.2	0.0	0.243247	12875	12938	214-215	1.000000	79.2
atpA-atpF_p3	atpA-atpF	3	ACTTGAAGTACGGTACCGGT	ATTGGCATGTTTGGGACGAT	20	20	50.0	45.0	58.4	58.1	35.9	0.0	0.527874	12875	12938	207-208	1.000000	76.9
atpA-atpF_p4	atpA-atpF	4	TTGCTCAATACGTTCACGGA	ATTGGCATGTTTGGGACGAT	20	20	45.0	45.0	57.6	58.1	4.1	0.0	0.590908	12875	12938	160-161	1.000000	76.8
atpA-atpF_p5	atpA-atpF	5	GTCGCCTACTTGAAGTACGG	TATTGGCATGTTTGGGACGA	20	20	55.0	45.0	58.1	57.5	13.2	0.0	0.593195	12875	12938	215-216	1.000000	76.4
ndhF_p1	ndhF	1	ACGAAGGGGACTAAACAGGA	AGCGGGAAATTTTTGTAATGCA	20	22	50.0	36.4	58.0	57.7	0.0	0.0	2.284268	112502	114748	2924-2924	0.500000	41.7
ndhF_p2	ndhF	2	ACAGGAACAAGAGGGATCCA	AGCGGGAAATTTTTGTAATGCA	20	22	50.0	36.4	58.0	57.7	15.2	0.0	2.309253	112502	114748	2910-2910	0.500000	41.5
ndhF_p3	ndhF	3	AAGAGGGATCCACCGAAGAA	AGCGGGAAATTTTTGTAATGCA	20	22	50.0	36.4	58.0	57.7	15.2	0.0	2.311839	112502	114748	2902-2902	0.500000	41.5
ndhF_p4	ndhF	4	CTCAGAAACGGAGGAAAGGG	AGCGGGAAATTTTTGTAATGCA	20	22	55.0	36.4	57.9	57.7	0.0	0.0	2.365065	112502	114748	2991-2991	0.500000	41.1
ndhF_p5	ndhF	5	GGGAAAGCGAGGAAGAAACA	AGCGGGAAATTTTTGTAATGCA	20	22	50.0	36.4	58.1	57.7	0.0	0.0	2.372251	112502	114748	2971-2971	0.500000	41.0
ndhF-rpl32_p1	ndhF-rpl32	1	AAAGTAGAAGTCCCGCTCCT	CGCCCTTTGCTTTTCCAAAT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.228346	114749	115402	813-813	0.500000	58.2
ndhF-rpl32_p2	ndhF-rpl32	2	GAAGGTTTTTGGTTGCCGTC	CGCCCTTTGCTTTTCCAAAT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.320508	114749	115402	837-837	0.500000	57.4
ndhF-rpl32_p3	ndhF-rpl32	3	GTCGAAGGTTTTTGGTTGCC	CGCCCTTTGCTTTTCCAAAT	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.320508	114749	115402	840-840	0.500000	57.4
ndhF-rpl32_p4	ndhF-rpl32	4	GTGGATCGATCAAGTAGCCC	CGCCCTTTGCTTTTCCAAAT	20	20	55.0	45.0	57.8	57.8	25.9	0.0	0.324669	114749	115402	1002-1002	0.500000	57.4
ndhF-rpl32_p5	ndhF-rpl32	5	TGGATCGATCAAGTAGCCCA	CGCCCTTTGCTTTTCCAAAT	20	20	50.0	45.0	58.2	57.8	25.9	0.0	0.380097	114749	115402	1001-1001	0.500000	57.0
rpl32-trnL-TAG_p1	rpl32-trnL-TAG	1	GGCAGCGTTGAAAGCTTTTT	GGAGAGCGGGGACTAATAGT	20	20	45.0	55.0	58.4	58.0	14.0	0.0	0.430965	115565	116725	1822-1822	0.500000	56.6
rpl32-trnL-TAG_p2	rpl32-trnL-TAG	2	GGCAGCGTTGAAAGCTTTTT	TGGAGAGCGGGGACTAATAG	20	20	45.0	55.0	58.4	58.0	14.0	0.0	0.432389	115565	116725	1823-1823	0.500000	56.5
rpl32-trnL-TAG_p3	rpl32-trnL-TAG	3	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.437017	115565	116725	1275-1275	0.500000	56.5
rpl32-trnL-TAG_p4	rpl32-trnL-TAG	4	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	58.4	58.0	14.0	0.0	0.438388	115565	116725	1276-1276	0.500000	56.5
rpl32-trnL-TAG_p5	rpl32-trnL-TAG	5	GGCAGCGTTGAAAGCTTTTT	CGGGGACTAATAGTGGGGAT	20	20	45.0	55.0	58.4	58.0	14.0	0.0	0.454823	115565	116725	1816-1816	0.500000	56.4
trnL-TAG_p1	trnL-TAG	1	GGAAACGCTCAAATCCCCTA	GGAGAGCGGGGACTAATAGT	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.415087	116726	116805	752-752	0.500000	56.7
trnL-TAG_p2	trnL-TAG	2	GGAAACGCTCAAATCCCCTA	TGGAGAGCGGGGACTAATAG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.416511	116726	116805	753-753	0.500000	56.7
trnL-TAG_p3	trnL-TAG	3	GGAAACGCTCAAATCCCCTA	CGGGGACTAATAGTGGGGAT	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.438945	116726	116805	746-746	0.500000	56.5
trnL-TAG_p4	trnL-TAG	4	GGAAACGCTCAAATCCCCTA	AAAATAGTACTTGGCGCGGT	20	20	50.0	45.0	57.6	57.9	0.0	8.8	0.516847	116726	116805	681-681	0.500000	55.9
trnL-TAG_p5	trnL-TAG	5	GGAAACGCTCAAATCCCCTA	AAATAGTACTTGGCGCGGTT	20	20	50.0	45.0	57.6	57.9	0.0	8.8	0.516847	116726	116805	680-680	0.500000	55.9
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030642	116902	117873	1731-1731	0.500000	59.8
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GGTATGAGCGGTTTTGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032012	116902	117873	1730-1730	0.500000	59.7
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.040512	116902	117873	1758-1758	0.500000	59.7
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.041882	116902	117873	1757-1757	0.500000	59.7
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	CATGTTTGTGTTCGTCAGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044387	116902	117873	1175-1175	0.500000	59.6
ndhD_p1	ndhD	1	GCCTGACGAACACAAACATG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.055300	118130	119539	1921-1921	0.500000	59.6
ndhD_p2	ndhD	2	GCCTGACGAACACAAACATG	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.067453	118130	119539	1927-1927	0.500000	59.5
ndhD_p3	ndhD	3	GCCTGACGAACACAAACATG	CCGACGGATTTCTTGAGTGT	20	20	50.0	50.0	58.0	57.9	0.0	8.6	0.116843	118130	119539	1961-1961	0.500000	59.1
ndhD_p4	ndhD	4	ATTCAAGAAAGGGCCTGACG	AAACAACTCGAAGCATGGGT	20	20	50.0	45.0	58.2	58.0	6.9	0.0	0.192359	118130	119539	1933-1933	0.500000	58.5
ndhD_p5	ndhD	5	ATTCAAGAAAGGGCCTGACG	GGCATGAAACAACTCGAAGC	20	20	50.0	50.0	58.2	58.0	6.9	0.0	0.204512	118130	119539	1939-1939	0.500000	58.4
ndhE_p1	ndhE	1	ACCCATGCTTCGAGTTGTTT	TTCGTTGGGACTGGTTCTTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.056950	120277	120579	1438-1438	0.500000	59.5
ndhE_p2	ndhE	2	GCTTCGAGTTGTTTCATGCC	TTCGTTGGGACTGGTTCTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.069103	120277	120579	1432-1432	0.500000	59.4
ndhE_p3	ndhE	3	ACACTCAAGAAATCCGTCGG	TTCGTTGGGACTGGTTCTTG	20	20	50.0	50.0	57.9	58.0	4.4	0.0	0.118493	120277	120579	1398-1398	0.500000	59.1
ndhE_p4	ndhE	4	CTTCTGTTCTTGGAGCCGAA	TTCGTTGGGACTGGTTCTTG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.208039	120277	120579	1343-1343	0.500000	58.3
ndhE_p5	ndhE	5	TTCTGTTCTTGGAGCCGAAG	TTCGTTGGGACTGGTTCTTG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.208039	120277	120579	1342-1342	0.500000	58.3
ndhE-ndhG_p1	ndhE-ndhG	1	ACAATAGCTGGGCCAATAGC	TTTGCCTGGACCGATACATG	20	20	50.0	50.0	58.0	58.0	8.0	0.0	0.050075	120580	120807	993-993	0.500000	59.6
ndhE-ndhG_p2	ndhE-ndhG	2	ACAATAGCTGGGCCAATAGC	TTCGTTGGGACTGGTTCTTG	20	20	50.0	50.0	58.0	58.0	8.0	0.0	0.051324	120580	120807	888-888	0.500000	59.6
ndhE-ndhG_p3	ndhE-ndhG	3	ACAATAGCTGGGCCAATAGC	TAATGGATTTGCCTGGACCG	20	20	50.0	50.0	58.0	57.9	8.0	0.0	0.064452	120580	120807	1000-1000	0.500000	59.5
ndhE-ndhG_p4	ndhE-ndhG	4	ACACTCAAGAAATCCGTCGG	TTTGCCTGGACCGATACATG	20	20	50.0	50.0	57.9	58.0	4.4	0.0	0.117244	120580	120807	1503-1503	0.500000	59.1
ndhE-ndhG_p5	ndhE-ndhG	5	ACACTCAAGAAATCCGTCGG	TTCGTTGGGACTGGTTCTTG	20	20	50.0	50.0	57.9	58.0	4.4	0.0	0.118493	120580	120807	1398-1398	0.500000	59.1
ndhG_p1	ndhG	1	ACAATAGCTGGGCCAATAGC	CTCCCCGTTGTTGATTGGAA	20	20	50.0	50.0	58.0	58.1	8.0	0.0	0.108831	120808	121338	1619-1619	0.500000	59.1
ndhG_p2	ndhG	2	ACAATAGCTGGGCCAATAGC	ATCTCCCCGTTGTTGATTGG	20	20	50.0	50.0	58.0	57.9	8.0	0.0	0.141888	120808	121338	1621-1621	0.500000	58.9
ndhG_p3	ndhG	3	ACAATAGCTGGGCCAATAGC	ACTGCGTTGAATATTGCCCA	20	20	50.0	45.0	58.0	58.2	8.0	0.0	0.180234	120808	121338	1525-1525	0.500000	58.6
ndhG_p4	ndhG	4	TGCGATAGGGCCAAGATACT	CTCCCCGTTGTTGATTGGAA	20	20	50.0	50.0	58.3	58.1	6.2	0.0	0.378681	120808	121338	1798-1798	0.500000	57.0
ndhG_p5	ndhG	5	ACAATAGCTGGGCCAATAGC	TCCCCGTTGTTGATTGGAAA	20	20	50.0	45.0	58.0	57.6	8.0	0.0	0.379758	120808	121338	1618-1618	0.500000	57.0
ndhG-ndhI_p1	ndhG-ndhI	1	AAACTGGGCGTTTGCTAGAA	AACAAACAGTACGAGCTGCA	20	20	45.0	45.0	58.0	58.1	0.0	1.0	0.076236	121339	121689	986-986	0.500000	59.4
ndhG-ndhI_p2	ndhG-ndhI	2	AAACTGGGCGTTTGCTAGAA	ACAAACAGTACGAGCTGCAA	20	20	45.0	45.0	58.0	58.1	0.0	1.0	0.076236	121339	121689	985-985	0.500000	59.4
ndhG-ndhI_p3	ndhG-ndhI	3	CATGTATCGGTCCAGGCAAA	AACAAACAGTACGAGCTGCA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.088853	121339	121689	840-840	0.500000	59.3
ndhG-ndhI_p4	ndhG-ndhI	4	CATGTATCGGTCCAGGCAAA	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.088853	121339	121689	839-839	0.500000	59.3
ndhG-ndhI_p5	ndhG-ndhI	5	CAAGAACCAGTCCCAACGAA	ACAAACAGTACGAGCTGCAA	20	20	50.0	45.0	58.0	58.1	0.0	1.0	0.090102	121339	121689	944-944	0.500000	59.3
ndhI_p1	ndhI	1	AAACTGGGCGTTTGCTAGAA	AAATAATCCCGACACGCTCC	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.074875	121690	122190	1762-1762	0.500000	59.4
ndhI_p2	ndhI	2	CATGTATCGGTCCAGGCAAA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.087492	121690	122190	1616-1616	0.500000	59.3
ndhI_p3	ndhI	3	CAAGAACCAGTCCCAACGAA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088741	121690	122190	1721-1721	0.500000	59.3
ndhI_p4	ndhI	4	CGGTCCAGGCAAATCCATTA	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.101869	121690	122190	1609-1609	0.500000	59.2
ndhI_p5	ndhI	5	AAACTGGGCGTTTGCTAGAA	TGGTTTTTGGGGGTGGAATT	20	20	45.0	45.0	58.0	57.8	0.0	0.0	0.222674	121690	122190	1593-1593	0.500000	58.2
ndhI-ndhA_p1	ndhI-ndhA	1	TGCAGCTCGTACTGTTTGTT	AAATAATCCCGACACGCTCC	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.102430	122191	122270	796-796	0.500000	59.2
ndhI-ndhA_p2	ndhI-ndhA	2	TTGCAGCTCGTACTGTTTGT	AAATAATCCCGACACGCTCC	20	20	45.0	50.0	58.1	58.1	8.8	0.0	0.102430	122191	122270	797-797	0.500000	59.2
ndhI-ndhA_p3	ndhI-ndhA	3	TTCCAATCAACAACGGGGAG	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.146248	122191	122270	990-990	0.500000	58.8
ndhI-ndhA_p4	ndhI-ndhA	4	CCAATCAACAACGGGGAGAT	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.179305	122191	122270	988-988	0.500000	58.6
ndhI-ndhA_p5	ndhI-ndhA	5	ACCAATGTACCTTGCAGCTC	AAATAATCCCGACACGCTCC	20	20	50.0	50.0	58.2	58.1	5.4	0.0	0.224150	122191	122270	808-808	0.500000	58.2
ndhA_p1	ndhA	1	TGCAGCTCGTACTGTTTGTT	CAGACTTGGTGAGATGACGG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.053708	122271	124517	2779-2779	0.500000	59.6
ndhA_p2	ndhA	2	TTGCAGCTCGTACTGTTTGT	CAGACTTGGTGAGATGACGG	20	20	45.0	55.0	58.1	58.0	8.8	0.0	0.053708	122271	124517	2780-2780	0.500000	59.6
ndhA_p3	ndhA	3	TGCAGCTCGTACTGTTTGTT	ATTGGGGGTTATCAGGACCA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	122271	124517	2921-2921	0.500000	59.5
ndhA_p4	ndhA	4	TGCAGCTCGTACTGTTTGTT	TGGGGGTTATCAGGACCAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	122271	124517	2919-2919	0.500000	59.5
ndhA_p5	ndhA	5	TTGCAGCTCGTACTGTTTGT	TGGGGGTTATCAGGACCAAT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.057497	122271	124517	2920-2920	0.500000	59.5
ndhH_p1	ndhH	1	ATCCGTTGTTGTATCCCTGC	CGGAAAACGCCAACGATTAC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.165092	124519	125700	1574-1574	0.500000	58.7
ndhH_p2	ndhH	2	ATCCGTTGTTGTATCCCTGC	TGGCACTGTCTGACATTCTG	20	20	50.0	50.0	58.0	57.8	0.0	11.5	0.212750	124519	125700	2094-2094	0.500000	58.3
ndhH_p3	ndhH	3	GATGGGAGCCACACTTGAAA	CGGAAAACGCCAACGATTAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.217005	124519	125700	1818-1818	0.500000	58.3
ndhH_p4	ndhH	4	TCAGGTCCAATCCGTTGTTG	CGGAAAACGCCAACGATTAC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.235829	124519	125700	1583-1583	0.500000	58.1
ndhH_p5	ndhH	5	ATCCGTTGTTGTATCCCTGC	TCGGAAAACGCCAACGATTA	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.245129	124519	125700	1575-1575	0.500000	58.0
rps15-ycf1_p1	rps15-ycf1	1	ATGAGTCGAAGAACACCGTG	TTTTATCCCCCAGAGGTCGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104860	126077	126503	1200-1200	0.500000	59.2
rps15-ycf1_p2	rps15-ycf1	2	GTAATCGTTGGCGTTTTCCG	TTTTATCCCCCAGAGGTCGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.165572	126077	126503	928-928	0.500000	58.7
rps15-ycf1_p3	rps15-ycf1	3	ATGAGTCGAAGAACACCGTG	CCCAGAGGTCGTAGAGAGTT	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.197768	126077	126503	1192-1192	0.500000	58.4
rps15-ycf1_p4	rps15-ycf1	4	CGTTTCTTGTAGCTGGTCCA	TTTTATCCCCCAGAGGTCGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.202685	126077	126503	1139-1139	0.500000	58.4
rps15-ycf1_p5	rps15-ycf1	5	GGTCGTTTCTTGTAGCTGGT	TTTTATCCCCCAGAGGTCGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.204186	126077	126503	1142-1142	0.500000	58.4
ycf1_p1	ycf1	1	CGAGCCTTGGATCATTCGAT	CCTTTTGGTTCTCCCCGAAA	20	20	50.0	50.0	57.8	58.0	5.7	0.0	0.171116	129240	129359	345-345	0.500000	58.6
ycf1_p2	ycf1	2	CGAGCCTTGGATCATTCGAT	ACGGTATTCCCAACAATCGG	20	20	50.0	50.0	57.8	58.0	5.7	0.0	0.193503	129240	129359	1060-1060	0.500000	58.5
ycf1_p3	ycf1	3	CGAGCCTTGGATCATTCGAT	AATTTCCCGAATGGTCCGAG	20	20	50.0	50.0	57.8	58.0	5.7	0.0	0.199209	129240	129359	608-608	0.500000	58.4
ycf1_p4	ycf1	4	CGAGCCTTGGATCATTCGAT	CCGAATGGTCCGAGGATTTT	20	20	50.0	50.0	57.8	58.0	5.7	0.0	0.199209	129240	129359	602-602	0.500000	58.4
ycf1_p5	ycf1	5	CGAGCCTTGGATCATTCGAT	TACACAGGAAAAGACAGGCG	20	20	50.0	50.0	57.8	57.8	5.7	0.0	0.324640	129240	129359	832-832	0.500000	57.4