primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	85	549	337-532	1.000000	90.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	85	549	351-546	1.000000	90.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	85	549	350-545	1.000000	90.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	85	549	352-547	1.000000	90.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	85	549	340-535	1.000000	90.4
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CAGTTCAGGATCAGTCGTGG	AATTGATGTGCGATCCCGAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.038769	4443	5333	1225-1248	1.000000	84.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTTCAGGATCAGTCGTGGTC	AATTGATGTGCGATCCCGAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.039288	4443	5333	1223-1246	1.000000	84.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CAGTTCAGGATCAGTCGTGG	ATCGTTGCAATTGATGTGCG	20	20	55.0	45.0	58.0	58.1	0.0	10.3	0.102874	4443	5333	1233-1256	1.000000	84.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTTCAGGATCAGTCGTGG	CGTTGCAATTGATGTGCGAT	20	20	55.0	45.0	58.0	58.1	0.0	10.3	0.102874	4443	5333	1231-1254	1.000000	84.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GTTCAGGATCAGTCGTGGTC	CGTTGCAATTGATGTGCGAT	20	20	55.0	45.0	58.0	58.1	0.0	10.3	0.103393	4443	5333	1229-1252	1.000000	84.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	6466	7923	871-1526	1.000000	86.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCTGTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.292817	6466	7923	887-1542	1.000000	86.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AGTCGCACGTTGCTTTTTAC	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.6	58.1	8.1	16.9	0.454608	6466	7923	875-1530	1.000000	84.9
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCACAGCTGATCATGTCTGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.6	58.1	23.3	16.9	0.474353	6466	7923	897-1552	1.000000	84.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAAGTCGCACGTTGCTTTTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.5	58.1	8.1	16.9	0.531415	6466	7923	877-1532	1.000000	84.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	9270	10083	877-922	1.000000	57.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	9270	10083	876-921	1.000000	57.1
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	9270	10083	930-975	1.000000	55.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CAATCCGACGCTTTAGTCCA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.0	56.2	0.0	0.0	3.780896	9270	10083	878-923	1.000000	55.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AATCCGACGCTTTAGTCCAC	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.0	56.2	0.0	0.0	3.782284	9270	10083	877-922	1.000000	55.1
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.404255	10871	11045	243-262	1.000000	52.9
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	AGCCTTCCAAGCTAACGATG	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.0	54.6	0.0	0.0	4.434337	10871	11045	237-256	1.000000	52.7
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTATGTCCT	20	22	55.0	40.9	58.1	55.6	0.0	0.0	4.433092	10871	11045	246-265	1.000000	52.6
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGAAGACCTATGTCCTATCCA	20	21	55.0	42.9	58.1	54.6	0.0	0.0	4.463174	10871	11045	240-259	1.000000	52.4
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	AGCCTTCCAAGCTAACGATG	ACCAAAGGTTTAGAAGACCTATGT	20	24	50.0	37.5	58.0	57.6	0.0	0.0	4.479668	10871	11045	248-267	1.000000	52.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCATTTCCATCCTCGAGCAT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.7	58.1	7.0	0.0	0.349302	28341	29634	1323-1376	1.000000	83.5
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CCATTTCCATCCTCGAGCAT	TTGTTAATGAGGCGACACCC	20	20	50.0	50.0	57.7	58.2	7.0	0.0	0.451100	28341	29634	1341-1394	1.000000	82.6
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ACATTCCCCCATTTCCATCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.5	58.1	0.0	0.0	0.559232	28341	29634	1331-1384	1.000000	81.8
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCATTTCCATCCTCGAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	57.7	58.4	7.0	14.6	0.622665	28341	29634	1305-1358	1.000000	81.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	ACATTCCCCCATTTCCATCC	TTGTTAATGAGGCGACACCC	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.661030	28341	29634	1349-1402	1.000000	80.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ACGCAGTCGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.7	58.5	0.0	5.4	0.756914	31744	32862	1222-1249	1.000000	80.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	CGCAGTCGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.4	58.5	0.0	5.4	1.039065	31744	32862	1221-1248	1.000000	78.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACGCAGTCGGAACTAGAATG	GTTCAATTGGTCAGAGCACC	20	20	50.0	50.0	57.7	57.0	0.0	5.4	1.277068	31744	32862	1223-1250	1.000000	76.1
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAAACGCAGTCGGAACTAGA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.2	58.5	0.0	5.4	1.283940	31744	32862	1225-1252	1.000000	76.1
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAACGCAGTCGGAACTAGAA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.2	58.5	0.0	5.4	1.283940	31744	32862	1224-1251	1.000000	76.1
accD_p1	accD	1	GTGCTTGTGATCGGATCCAT	TGAATTGCGCATAAGGCAGA	20	20	50.0	45.0	58.0	58.3	13.4	12.8	0.292008	61907	63562	1831-2003	1.000000	85.4
accD_p2	accD	2	TCCTAGTGCTTGTGATCGGA	TGAATTGCGCATAAGGCAGA	20	20	50.0	45.0	58.2	58.3	0.0	12.8	0.403721	61907	63562	1836-2008	1.000000	84.5
accD_p3	accD	3	GTGCTTGTGATCGGATCCAT	GCCTAATTTGAATGGCGCAT	20	20	50.0	45.0	58.0	57.5	13.4	10.8	0.555163	61907	63562	1852-2024	1.000000	83.3
accD_p4	accD	4	GTGCTTGTGATCGGATCCAT	TTGAATTGCGCATAAGGCAG	20	20	50.0	45.0	58.0	57.4	13.4	12.8	0.606169	61907	63562	1832-2004	1.000000	82.9
accD_p5	accD	5	ATTGTCCTTTTCGGTTCCGT	TGAATTGCGCATAAGGCAGA	20	20	45.0	45.0	57.7	58.3	11.0	12.8	0.516638	61907	63562	2422-2422	0.200000	43.9
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGTCCGA	TTCATACTCCGGGTTAGGCT	21	20	42.9	50.0	57.2	57.8	5.1	0.0	1.932419	68063	69023	994-1035	1.000000	70.8
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGTCCGA	GTTCATACTCCGGGTTAGGC	21	20	42.9	55.0	57.2	57.8	5.1	0.0	1.998252	68063	69023	995-1036	1.000000	70.3
petA-psbJ_p3	petA-psbJ	3	TGAGAAGGTTCAATTGTCCGA	TACTCCGGGTTAGGCTCATC	21	20	42.9	55.0	57.2	58.3	5.1	0.0	2.061452	68063	69023	990-1031	1.000000	69.8
petA-psbJ_p4	petA-psbJ	4	TGAGAAGGTTCAATTGTCCGA	TCATACTCCGGGTTAGGCTC	21	20	42.9	55.0	57.2	58.3	5.1	0.0	2.061452	68063	69023	993-1034	1.000000	69.8
petA-psbJ_p5	petA-psbJ	5	TGAGAAGGTTCAATTGTCCGA	ATACTCCGGGTTAGGCTCAT	21	20	42.9	50.0	57.2	57.6	5.1	0.0	2.162874	68063	69023	991-1032	1.000000	69.0
rps12-clpP_p1	rps12-clpP	1	CTGAACGAGTCGCACATACA	CCGAAGATCTGGAGAGGGAT	20	20	50.0	55.0	58.0	58.0	13.6	0.0	0.031054	75534	75674	350-478	1.000000	87.8
rps12-clpP_p2	rps12-clpP	2	ACTCTCTATACACCGGAGCC	TGGGCGAATTTGTCTTGGAA	20	20	55.0	45.0	58.0	58.0	9.5	0.0	0.027641	75534	75674	914-919	0.800000	76.1
rps12-clpP_p3	rps12-clpP	3	ACTCTCTATACACCGGAGCC	CCGAAGATCTGGAGAGGGAT	20	20	55.0	55.0	58.0	58.0	9.5	0.0	0.024177	75534	75674	815-820	0.800000	75.7
rps12-clpP_p4	rps12-clpP	4	CTGAACGAGTCGCACATACA	ATCCGAAGATCTGGAGAGGG	20	20	50.0	55.0	58.0	58.0	13.6	7.8	0.031054	75534	75674	352-353	0.600000	67.2
rps12-clpP_p5	rps12-clpP	5	ACTCTCTATACACCGGAGCC	ATCCGAAGATCTGGAGAGGG	20	20	55.0	55.0	58.0	58.0	9.5	7.8	0.024177	75534	75674	817-822	0.600000	66.0
clpP_p1	clpP	1	CTGAACGAGTCGCACATACA	ATACGCAATGGGGGTTGATC	20	20	50.0	50.0	58.0	58.0	13.6	1.6	0.041555	75675	77924	2534-2725	1.000000	90.2
clpP_p2	clpP	2	ATTTTGTCCCAGCGAGGATC	ATACGCAATGGGGGTTGATC	20	20	50.0	50.0	58.0	58.0	0.8	1.6	0.062866	75675	77924	2553-2744	1.000000	90.0
clpP_p3	clpP	3	CTGAACGAGTCGCACATACA	ACTATGCTATGGACCTCCCC	20	20	50.0	55.0	58.0	58.0	13.6	0.0	0.054307	75675	77924	2680-2871	1.000000	89.8
clpP_p4	clpP	4	CTGAACGAGTCGCACATACA	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.041529	75675	77924	2805-2996	1.000000	89.6
clpP_p5	clpP	5	ATTTTGTCCCAGCGAGGATC	GAACAGAAAGCAAACGACCG	20	20	50.0	50.0	58.0	58.0	0.8	0.0	0.062840	75675	77924	2824-2995	0.800000	79.2
rps8-rpl14_p1	rps8-rpl14	1	AGCAATAGTGTCCCTACCCA	AGTTTCATTAGCACCTGAGGT	20	21	50.0	42.9	57.7	56.9	0.0	0.0	2.341951	86470	86715	274-333	1.000000	69.2
rps8-rpl14_p2	rps8-rpl14	2	AGCAATAGTGTCCCTACCCA	GTTTCATTAGCACCTGAGGT	20	20	50.0	45.0	57.7	55.4	0.0	0.0	2.911567	86470	86715	273-332	1.000000	64.7
rps8-rpl14_p3	rps8-rpl14	3	AGCAATAGTGTCCCTACCCA	AGTTTCATTAGCACCTGAGG	20	20	50.0	45.0	57.7	55.0	0.0	0.0	3.215262	86470	86715	274-333	1.000000	62.2
rps8-rpl14_p4	rps8-rpl14	4	AGCAATAGTGTCCCTACCCAT	AGTTTCATTAGCACCTGAGGT	21	21	47.6	42.9	58.2	56.9	0.0	0.0	3.301391	86470	86715	274-333	1.000000	61.6
rps8-rpl14_p5	rps8-rpl14	5	GCAATAGTGTCCCTACCCAT	AGTTTCATTAGCACCTGAGGT	20	21	50.0	42.9	56.7	56.9	0.0	0.0	3.403221	86470	86715	273-332	1.000000	60.8
ccsA-ndhD_p1	ccsA-ndhD	1	AGGGTTACATAGTTATGGTTCA	TCAGTTGATAAGGTGGAAGC	22	20	36.4	45.0	54.2	55.1	4.3	0.0	8.707726	120814	121203	469-479	1.000000	44.7
ccsA-ndhD_p2	ccsA-ndhD	2	AGGGTTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTTTCA	22	20	36.4	40.0	54.2	55.1	4.3	12.4	8.694472	120814	121203	492-502	1.000000	44.5
ccsA-ndhD_p3	ccsA-ndhD	3	AGGGTTACATAGTTATGGTTCA	TCCGGATTTCGTTCTTTCAT	22	20	36.4	40.0	54.2	55.1	4.3	0.0	8.694472	120814	121203	491-501	1.000000	44.5
ccsA-ndhD_p4	ccsA-ndhD	4	AGGGTTACATAGTTATGGTTCA	TCCGGATTTCGTTCTTTCATT	22	21	36.4	38.1	54.2	55.9	4.3	0.0	8.882845	120814	121203	491-501	1.000000	44.5
ccsA-ndhD_p5	ccsA-ndhD	5	AGGGTTACATAGTTATGGTTCA	ATCCGGATTTCGTTCTTTCAT	22	21	36.4	38.1	54.2	55.7	4.3	12.4	9.105081	120814	121203	492-502	1.000000	44.5
ycf1_p1	ycf1	1	TCTCTACGCCGTTTAGTGGA	CGAAGGGATTGCAACGACTA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.179492	129871	129990	258-264	0.800000	79.2
ycf1_p2	ycf1	2	GATTTGCCCAAGGTAGCCTT	CGAAGGGATTGCAACGACTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.151950	129871	129990	551-551	0.200000	46.8
ycf1_p3	ycf1	3	GATTTGCCCAAGGTAGCCTT	TAGCCTTTCTTCGAGGGGAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.200383	129871	129990	648-648	0.200000	46.4
ycf1_p4	ycf1	4	GATTTGCCCAAGGTAGCCTT	GGGGGAGCTAGTTTTTAGGC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.201600	129871	129990	599-599	0.200000	46.4
ycf1_p5	ycf1	5	ATGGATTTGCCCAAGGTAGC	CGAAGGGATTGCAACGACTA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.224080	129871	129990	554-554	0.200000	46.2