primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTGATCCA	TAGACCTAGCCGCTCTTGAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.272605	62	400	238-575	1.000000	89.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTGATCCAC	TAGACCTAGCCGCTCTTGAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.274112	62	400	237-574	1.000000	89.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACAATCCACTGCCTTGATCC	TAGACCTAGCCGCTCTTGAA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.274112	62	400	239-576	1.000000	89.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CAATCCACTGCCTTGATCCA	CTAGACCTAGCCGCTCTTGA	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.451743	62	400	239-576	1.000000	88.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	AATCCACTGCCTTGATCCAC	CTAGACCTAGCCGCTCTTGA	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.453251	62	400	238-575	1.000000	88.4
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	ACTTGTGTTGGATTGGCACT	GCGGAGGCTTTTAAGGAACT	20	20	45.0	50.0	57.9	58.2	0.0	0.0	0.249272	4792	4911	171-181	1.000000	90.0
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	GACTTGTGTTGGATTGGCAC	GCGGAGGCTTTTAAGGAACT	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.599770	4792	4911	172-182	1.000000	87.2
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	AGACTTGTGTTGGATTGGCA	GCGGAGGCTTTTAAGGAACT	20	20	45.0	50.0	57.6	58.2	0.0	0.0	0.540800	4792	4911	173-183	0.987500	87.2
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	TGTGTTGGATTGGCACTACA	GCGGAGGCTTTTAAGGAACT	20	20	45.0	50.0	57.4	58.2	0.0	0.0	0.825177	4792	4911	168-178	1.000000	85.4
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	TTGTGTTGGATTGGCACTACA	GCGGAGGCTTTTAAGGAACT	21	20	42.9	50.0	58.1	58.2	0.0	0.0	1.239975	4792	4911	169-179	1.000000	82.1
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	20	22	45.0	40.9	58.0	57.7	0.0	0.0	2.339278	9090	9834	827-892	0.987500	72.8
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	GCTCGTACAACGGATTAGCA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.1	57.7	0.0	0.0	2.383799	9090	9834	840-905	0.987500	72.4
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.2	57.7	0.0	0.0	2.452420	9090	9834	876-941	0.987500	71.9
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	AGCTCGTACAACGGATTAGC	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	57.8	57.7	0.0	0.0	2.506379	9090	9834	841-906	0.987500	71.4
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	TGGAAACGGAAAGAGAGGGA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.3	57.7	0.0	0.0	2.576527	9090	9834	881-946	0.987500	70.9
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	20	20	45.0	45.0	58.0	58.5	1.4	0.0	0.556109	14484	15635	1235-1273	1.000000	87.6
atpH-atpI_p2	atpH-atpI	2	GTTCCTTGACCAACTCCAGG	TTTTTGCAACTTTAGCCGCG	20	20	55.0	45.0	58.1	58.5	0.0	0.0	0.620163	14484	15635	1292-1330	1.000000	87.0
atpH-atpI_p3	atpH-atpI	3	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	14484	15635	1208-1246	1.000000	87.0
atpH-atpI_p4	atpH-atpI	4	CAGCAGTTCCTTGACCAACT	TTTTTGCAACTTTAGCCGCG	20	20	50.0	45.0	58.0	58.5	0.0	0.0	0.544850	14484	15635	1297-1335	0.950000	85.5
atpH-atpI_p5	atpH-atpI	5	CAGCAGTTCCTTGACCAACT	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	58.0	57.4	0.0	21.3	0.614188	14484	15635	1270-1308	0.950000	85.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGATCAACTCCCTTTCCCAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035809	32981	33760	545-1036	1.000000	91.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGATCAACTCCCTTTCCCAG	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	58.0	58.2	0.0	0.0	0.180742	32981	33760	542-1033	1.000000	90.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGATCAACTCCCTTTCCCAG	ACTCTACCGCTGACTGAGTT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.117421	32981	33760	514-1005	0.987500	90.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TCCCTTTCCCAGTACCCTAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.7	58.0	0.0	0.0	0.264984	32981	33760	537-1028	1.000000	89.9
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGATCAACTCCCTTTCCCAG	CGTTACTCTACCGCTGACTG	20	20	55.0	55.0	58.0	57.8	0.0	0.0	0.205368	32981	33760	518-1009	0.987500	89.9
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48270	49025	796-832	0.987500	89.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48270	49025	788-824	0.975000	89.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	48270	49025	802-838	1.000000	89.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48270	49025	828-864	0.975000	88.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48270	49025	820-856	0.962500	88.5
rbcL-accD_p1	rbcL-accD	1	CTTGCTCGTGACGGTAATGA	CCCCCATAAACCCAAACTCC	20	20	50.0	55.0	58.0	58.1	4.7	0.0	0.148560	58390	60723	2158-2997	0.862500	85.3
rbcL-accD_p2	rbcL-accD	2	TCTTGCTCGTGACGGTAATG	CCCCCATAAACCCAAACTCC	20	20	50.0	55.0	58.0	58.1	4.7	0.0	0.148560	58390	60723	2159-2998	0.862500	85.3
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	TACCAAAACTGGCTGTCACC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.138201	58390	60723	2341-2999	0.475000	69.9
rbcL-accD_p4	rbcL-accD	4	CTTGCTCGTGACGGTAATGA	CCACCGGTAGTAGGAGATGT	20	20	50.0	55.0	58.0	57.9	4.7	6.9	0.078035	58390	60723	2381-2999	0.462500	69.9
rbcL-accD_p5	rbcL-accD	5	TCTTGCTCGTGACGGTAATG	CCACCGGTAGTAGGAGATGT	20	20	50.0	55.0	58.0	57.9	4.7	6.9	0.078035	58390	60723	2382-3000	0.462500	69.9
petA-psbJ_p1	petA-psbJ	1	CGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCG	20	20	50.0	50.0	56.8	58.0	5.1	4.1	1.195174	65988	66997	1047-1105	0.987500	81.9
petA-psbJ_p2	petA-psbJ	2	CGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCG	21	20	47.6	50.0	58.3	58.0	5.1	4.1	1.348714	65988	66997	1047-1105	0.987500	80.7
petA-psbJ_p3	petA-psbJ	3	TCGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCG	21	20	47.6	50.0	58.3	58.0	5.1	4.1	1.348714	65988	66997	1048-1106	0.987500	80.7
petA-psbJ_p4	petA-psbJ	4	CGAGAAGGTTCAATTGTCCG	GGATTGGGTTCATCGCTGTA	20	20	50.0	50.0	56.8	57.7	5.1	0.0	1.488076	65988	66997	1042-1100	0.987500	79.6
petA-psbJ_p5	petA-psbJ	5	CGAGAAGGTTCAATTGTCCG	AGCTGGGATTCTTGTGATCG	20	20	50.0	50.0	56.8	57.7	5.1	0.0	1.484848	65988	66997	1097-1155	0.975000	79.1
rps12-psbB_p1	rps12-psbB	1	GGGGGCTTTTGTGACATTTC	ATGGACAGAAAGCAGTCGAC	20	20	50.0	50.0	57.5	57.9	0.0	7.7	0.541728	73544	74725	1294-1338	1.000000	85.8
rps12-psbB_p2	rps12-psbB	2	GGGGGCTTTTGTGACATTTC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.5	58.2	0.0	0.0	0.692966	73544	74725	1245-1289	1.000000	84.8
rps12-psbB_p3	rps12-psbB	3	GGGGGCTTTTGTGACATTTC	GTCGACCGGGATCATTCAAT	20	20	50.0	50.0	57.5	57.8	0.0	6.3	0.696167	73544	74725	1280-1324	0.987500	84.0
rps12-psbB_p4	rps12-psbB	4	GTACATGTTCCTCGACGCTG	ATGGACAGAAAGCAGTCGAC	20	20	55.0	50.0	58.7	57.9	2.3	7.7	0.816888	73544	74725	1332-1376	1.000000	83.3
rps12-psbB_p5	rps12-psbB	5	AGTACATGTTCCTCGACGCT	ATGGACAGAAAGCAGTCGAC	20	20	50.0	50.0	58.8	57.9	2.3	7.7	0.911271	73544	74725	1333-1377	1.000000	82.5
infA-rps8_p1	infA-rps8	1	TTGGACTCTATCGCCTGGAA	CGGGATGGGGGTTGTAATTC	20	20	50.0	55.0	58.1	58.3	0.0	0.0	0.451796	82314	82493	376-407	0.900000	82.9
infA-rps8_p2	infA-rps8	2	TTGGACTCTATCGCCTGGAA	TCGGCGGAGAAATTTTGTGT	20	20	50.0	45.0	58.1	58.4	0.0	7.2	0.539336	82314	82493	305-336	0.900000	82.8
infA-rps8_p3	infA-rps8	3	TTGGACTCTATCGCCTGGAA	CGGCGGAGAAATTTTGTGTT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.555056	82314	82493	304-335	0.900000	82.7
infA-rps8_p4	infA-rps8	4	TTGGACTCTATCGCCTGGAA	AGAATTTTAGGCGGGATGGG	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.504331	82314	82493	387-418	0.900000	82.4
infA-rps8_p5	infA-rps8	5	TTGGACTCTATCGCCTGGAA	TCGACTAGAAAGAATCGGCG	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.609555	82314	82493	319-350	0.875000	81.1
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CCGAACGCTCTACCACTAAG	GCATATTGATATGTCTGTTCCA	20	22	55.0	36.4	57.8	54.1	0.0	11.5	6.138220	111246	112221	861-1450	0.925000	49.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	CCCCAAGTAGGATTCGAACC	GCATATTGATATGTCTGTTCCA	20	22	55.0	36.4	57.7	54.1	0.0	11.5	6.275095	111246	112221	901-1490	0.925000	49.0
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CGAACGCTCTACCACTAAGC	GCATATTGATATGTCTGTTCCA	20	22	55.0	36.4	58.5	54.1	0.0	11.5	6.401061	111246	112221	860-1449	0.925000	49.0
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	CCGAACGCTCTACCACTAAG	GCATATTGATATGTCTGTTCCAT	20	23	55.0	34.8	57.8	54.6	0.0	11.5	6.557038	111246	112221	861-1450	0.925000	49.0
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CCCCAAGTAGGATTCGAACC	GCATATTGATATGTCTGTTCCAT	20	23	55.0	34.8	57.7	54.6	0.0	11.5	6.693913	111246	112221	901-1490	0.925000	49.0
ndhF-trnL-UAG_p1	ndhF-trnL-UAG	1	TCTTGTATTTATCGTATGTATCCA	AGCGTGTCTACCGATTTCAC	24	20	29.2	50.0	53.2	58.0	0.0	0.0	8.817543	114484	115550	941-1199	1.000000	52.0
ndhF-trnL-UAG_p2	ndhF-trnL-UAG	2	TCTTGTATTTATCGTATGTATCCA	CAGCGTGTCTACCGATTTCA	24	20	29.2	50.0	53.2	58.0	0.0	0.0	8.818913	114484	115550	942-1200	1.000000	52.0
ndhF-trnL-UAG_p3	ndhF-trnL-UAG	3	TCTTGTATTTATCGTATGTATCCA	TCCTAAGAGCAGCGTGTCTA	24	20	29.2	50.0	53.2	57.9	0.0	0.0	8.933989	114484	115550	951-1209	1.000000	52.0
ndhF-trnL-UAG_p4	ndhF-trnL-UAG	4	TCTTGTATTTATCGTATGTATCCA	CCTAAGAGCAGCGTGTCTAC	24	20	29.2	55.0	53.2	57.8	0.0	0.0	9.006007	114484	115550	950-1208	1.000000	52.0
ndhF-trnL-UAG_p5	ndhF-trnL-UAG	5	TCTTGTATTTATCGTATGTATCCA	CTAAGAGCAGCGTGTCTACC	24	20	29.2	55.0	53.2	57.8	0.0	0.0	9.006007	114484	115550	949-1207	1.000000	52.0
ndhH-trnN-GUU_p1	ndhH-trnN-GUU	1	GATACCCACGCCATCCTTTT	CGATTGGGACTTGAGACAGA	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.091204	131520	131639	366-670	0.237500	52.8
ndhH-trnN-GUU_p2	ndhH-trnN-GUU	2	GATACCCACGCCATCCTTTT	TCGATTGGGACTTGAGACAG	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.091204	131520	131639	367-671	0.237500	52.8
ndhH-trnN-GUU_p3	ndhH-trnN-GUU	3	TCCCAAGCGATCGATTTGAG	CGATTGGGACTTGAGACAGA	20	20	50.0	50.0	58.1	57.0	25.1	0.0	1.098448	131520	131639	561-609	0.200000	51.2
ndhH-trnN-GUU_p4	ndhH-trnN-GUU	4	TCCCAAGCGATCGATTTGAG	TCGATTGGGACTTGAGACAG	20	20	50.0	50.0	58.1	57.0	25.1	0.0	1.098448	131520	131639	562-610	0.200000	51.2
ndhH-trnN-GUU_p5	ndhH-trnN-GUU	5	GAACTTTTCGCGGAAGAACG	CGATTGGGACTTGAGACAGA	20	20	50.0	50.0	58.1	57.0	23.5	0.0	1.104965	131520	131639	290-303	0.125000	48.0