primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score petA-psbJ_p1 petA-psbJ 1 CCCATTACGTGTCCAAGGAC CATATTCCGGATTGGGGTCA 20 20 55.0 50.0 58.3 57.1 12.3 4.1 1.215014 61660 62522 1010-1010 1.000000 74.1 petA-psbJ_p2 petA-psbJ 2 GACCCATTACGTGTCCAAGG CATATTCCGGATTGGGGTCA 20 20 55.0 50.0 58.3 57.1 0.0 4.1 1.215014 61660 62522 1012-1012 1.000000 74.1 petA-psbJ_p3 petA-psbJ 3 ACGTGTCCAAGGACTTTTGT CATATTCCGGATTGGGGTCA 20 20 45.0 50.0 57.6 57.1 19.2 4.1 1.362486 61660 62522 1004-1004 1.000000 72.9 petA-psbJ_p4 petA-psbJ 4 CCCATTACGTGTCCAAGGAC GCTGGTATTCTTGTGATCGGT 20 21 55.0 47.6 58.3 58.1 12.3 0.0 1.359483 61660 62522 1056-1056 1.000000 72.8 petA-psbJ_p5 petA-psbJ 5 GACCCATTACGTGTCCAAGG GCTGGTATTCTTGTGATCGGT 20 21 55.0 47.6 58.3 58.1 0.0 0.0 1.359483 61660 62522 1058-1058 1.000000 72.8 ycf1_p1 ycf1 1 AGTGTCCCGTGTCAACTCTA CAACGTTTCAGCCTGGGATA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.116829 111825 111944 1051-1051 0.500000 59.1 ycf1_p2 ycf1 2 AGTGTCCCGTGTCAACTCTA AACGTTTCAGCCTGGGATAC 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.118304 111825 111944 1050-1050 0.500000 59.1 ycf1_p3 ycf1 3 AAAGTGTCCCGTGTCAACTC CAACGTTTCAGCCTGGGATA 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.158485 111825 111944 1053-1053 0.500000 58.7 ycf1_p4 ycf1 4 AAAGTGTCCCGTGTCAACTC AACGTTTCAGCCTGGGATAC 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.159959 111825 111944 1052-1052 0.500000 58.7 ycf1_p5 ycf1 5 AGTGTCCCGTGTCAACTCTA AGCTGCTCGCCTTGTTAAAT 20 20 50.0 45.0 58.0 58.2 0.0 0.0 0.182494 111825 111944 861-861 0.500000 58.5 rps15-ndhH_p1 rps15-ndhH 1 GCTGAAACGTTGTTGGTAGC TCCCAACGCGTTACATAAGG 20 20 50.0 50.0 58.0 58.0 0.0 26.1 0.045351 112883 113012 1001-1001 0.500000 59.6 rps15-ndhH_p2 rps15-ndhH 2 GCTGAAACGTTGTTGGTAGC CAGAAGCTCGTAGCATTGGT 20 20 50.0 50.0 58.0 58.0 0.0 9.1 0.048150 112883 113012 1471-1471 0.500000 59.6 rps15-ndhH_p3 rps15-ndhH 3 GCTGAAACGTTGTTGGTAGC GATGAGCCGAAGAACACCAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.062252 112883 113012 876-876 0.500000 59.5 rps15-ndhH_p4 rps15-ndhH 4 GCTGAAACGTTGTTGGTAGC CCGAGCCATAACAAATGGGA 20 20 50.0 50.0 58.0 57.9 0.0 6.2 0.083748 112883 113012 1145-1145 0.500000 59.3 rps15-ndhH_p5 rps15-ndhH 5 GCTGAAACGTTGTTGGTAGC AACACCATGCATTGATGGGT 20 20 50.0 45.0 58.0 58.1 0.0 8.9 0.091037 112883 113012 864-864 0.500000 59.3 ndhH_p1 ndhH 1 AAAAGATTTGTGCGTGTGCC ATTTCACCGAATCGCACGTA 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.149293 113013 114194 2456-2456 0.500000 58.8 ndhH_p2 ndhH 2 AAAAGATTTGTGCGTGTGCC TATTTCACCGAATCGCACGT 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.149293 113013 114194 2457-2457 0.500000 58.8 ndhH_p3 ndhH 3 AAAAGATTTGTGCGTGTGCC ACAAGGTGGTAGCCAAAAGG 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.152965 113013 114194 2254-2254 0.500000 58.8 ndhH_p4 ndhH 4 CTCAGAGAGGGTTGCGAAAA ATTTCACCGAATCGCACGTA 20 20 50.0 45.0 57.8 58.0 0.0 0.0 0.170784 113013 114194 2014-2014 0.500000 58.6 ndhH_p5 ndhH 5 CTCAGAGAGGGTTGCGAAAA TATTTCACCGAATCGCACGT 20 20 50.0 45.0 57.8 58.0 0.0 0.0 0.170784 113013 114194 2015-2015 0.500000 58.6 ndhA-ndhI_p1 ndhA-ndhI 1 TTTTGGGGGTGGAATCTGTG GTTGGGCAATACTCGACACA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.204908 116461 116536 909-909 0.500000 58.4 ndhA-ndhI_p2 ndhA-ndhI 2 TTTTGGGGGTGGAATCTGTG TGTTGGGCAATACTCGACAC 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.204908 116461 116536 910-910 0.500000 58.4 ndhA-ndhI_p3 ndhA-ndhI 3 GGTTTTTGGGGGTGGAATCT GTTGGGCAATACTCGACACA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.220842 116461 116536 912-912 0.500000 58.2 ndhA-ndhI_p4 ndhA-ndhI 4 GGTTTTTGGGGGTGGAATCT TGTTGGGCAATACTCGACAC 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.220842 116461 116536 913-913 0.500000 58.2 ndhA-ndhI_p5 ndhA-ndhI 5 CTCTAGCCGAGTGTGAGAGA GTTGGGCAATACTCGACACA 20 20 55.0 50.0 58.0 58.2 0.0 0.0 0.227924 116461 116536 851-851 0.500000 58.2 ndhI_p1 ndhI 1 TTTTGGGGGTGGAATCTGTG CAATCCCAGCGAAAAAGCAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.021545 116537 117040 1584-1584 0.500000 59.8 ndhI_p2 ndhI 2 TTTTGGGGGTGGAATCTGTG TGACCCCTAAACCCCCTAAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.032772 116537 117040 1540-1540 0.500000 59.7 ndhI_p3 ndhI 3 GGTTTTTGGGGGTGGAATCT CAATCCCAGCGAAAAAGCAG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.037478 116537 117040 1587-1587 0.500000 59.7 ndhI_p4 ndhI 4 CTCTAGCCGAGTGTGAGAGA CAATCCCAGCGAAAAAGCAG 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.044560 116537 117040 1526-1526 0.500000 59.6 ndhI_p5 ndhI 5 GGTTTTTGGGGGTGGAATCT TGACCCCTAAACCCCCTAAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.048705 116537 117040 1543-1543 0.500000 59.6 ndhI-ndhG_p1 ndhI-ndhG 1 CAACCTCGCCTCAACTTCTT CAATCCCAGCGAAAAAGCAG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.129067 117041 117435 1127-1127 0.500000 59.0 ndhI-ndhG_p2 ndhI-ndhG 2 CAACCTCGCCTCAACTTCTT TGACCCCTAAACCCCCTAAG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.140294 117041 117435 1083-1083 0.500000 58.9 ndhI-ndhG_p3 ndhI-ndhG 3 CAACCTCGCCTCAACTTCTT AAATCCGGTATTGGTTCGGG 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.162767 117041 117435 1033-1033 0.500000 58.7 ndhI-ndhG_p4 ndhI-ndhG 4 ATGGACTTTACCAAGGCTGC CAATCCCAGCGAAAAAGCAG 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.173878 117041 117435 1216-1216 0.500000 58.6 ndhI-ndhG_p5 ndhI-ndhG 5 CCTCGCCTCAACTTCTTTCA CAATCCCAGCGAAAAAGCAG 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.183134 117041 117435 1124-1124 0.500000 58.5 ndhG_p1 ndhG 1 GTGTCGAGTATTGCCCAACA TTGCTGAAACAATAGCCGGT 20 20 50.0 45.0 58.2 58.1 0.0 1.9 0.292379 117436 117966 1470-1470 0.500000 57.7 ndhG_p2 ndhG 2 TGTGTCGAGTATTGCCCAAC TTGCTGAAACAATAGCCGGT 20 20 50.0 45.0 58.2 58.1 0.0 1.9 0.292379 117436 117966 1471-1471 0.500000 57.7 ndhG_p3 ndhG 3 GTGTCGAGTATTGCCCAACA CTGAAACAATAGCCGGTCCA 20 20 50.0 50.0 58.2 57.9 0.0 1.9 0.304616 117436 117966 1467-1467 0.500000 57.6 ndhG_p4 ndhG 4 TGTGTCGAGTATTGCCCAAC CTGAAACAATAGCCGGTCCA 20 20 50.0 50.0 58.2 57.9 0.0 1.9 0.304616 117436 117966 1468-1468 0.500000 57.6 ndhG_p5 ndhG 5 GTGTCGAGTATTGCCCAACA ACAATAGCCGGTCCAATAGC 20 20 50.0 50.0 58.2 57.7 0.0 1.9 0.452783 117436 117966 1462-1462 0.500000 56.4 ndhG-ndhE_p1 ndhG-ndhE 1 CTGCTTTTTCGCTGGGATTG TTGCTGAAACAATAGCCGGT 20 20 50.0 45.0 58.0 58.1 0.0 1.9 0.109015 117967 118087 796-796 0.500000 59.1 ndhG-ndhE_p2 ndhG-ndhE 2 CTTAGGGGGTTTAGGGGTCA TTGCTGAAACAATAGCCGGT 20 20 55.0 45.0 58.0 58.1 0.0 1.9 0.120243 117967 118087 840-840 0.500000 59.0 ndhG-ndhE_p3 ndhG-ndhE 3 CTGCTTTTTCGCTGGGATTG CTGAAACAATAGCCGGTCCA 20 20 50.0 50.0 58.0 57.9 0.0 1.9 0.121252 117967 118087 793-793 0.500000 59.0 ndhG-ndhE_p4 ndhG-ndhE 4 CTGCTTTTTCGCTGGGATTG TGCTTTACATCCGTCCCAAG 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.121252 117967 118087 1285-1285 0.500000 59.0 ndhG-ndhE_p5 ndhG-ndhE 5 CTTAGGGGGTTTAGGGGTCA CTGAAACAATAGCCGGTCCA 20 20 55.0 50.0 58.0 57.9 0.0 1.9 0.132479 117967 118087 837-837 0.500000 58.9 ndhE_p1 ndhE 1 CTGCTTTTTCGCTGGGATTG CCAGAAAAACCAGTGCTCCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.026420 118088 118390 1519-1519 0.500000 59.8 ndhE_p2 ndhE 2 CTTAGGGGGTTTAGGGGTCA CCAGAAAAACCAGTGCTCCT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.037647 118088 118390 1563-1563 0.500000 59.7 ndhE_p3 ndhE 3 CTGCTTTTTCGCTGGGATTG AACCAACGCAGTCTTCTGTT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.051689 118088 118390 1328-1328 0.500000 59.6 ndhE_p4 ndhE 4 CCCGAACCAATACCGGATTT CCAGAAAAACCAGTGCTCCT 20 20 50.0 50.0 58.0 58.0 9.5 0.0 0.060119 118088 118390 1613-1613 0.500000 59.5 ndhE_p5 ndhE 5 CTTAGGGGGTTTAGGGGTCA AACCAACGCAGTCTTCTGTT 20 20 55.0 45.0 58.0 58.0 0.0 0.0 0.062916 118088 118390 1372-1372 0.500000 59.5 ndhE-psaC_p1 ndhE-psaC 1 ACCGGCTATTGTTTCAGCAA CCAGAAAAACCAGTGCTCCT 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.108831 118391 118715 743-743 0.500000 59.1 ndhE-psaC_p2 ndhE-psaC 2 TGGACCGGCTATTGTTTCAG CCAGAAAAACCAGTGCTCCT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.121068 118391 118715 746-746 0.500000 59.0 ndhE-psaC_p3 ndhE-psaC 3 ACCGGCTATTGTTTCAGCAA AACCAACGCAGTCTTCTGTT 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.134100 118391 118715 552-552 0.500000 58.9 ndhE-psaC_p4 ndhE-psaC 4 AGTTGCTTTAATAGGCGCCA CCAGAAAAACCAGTGCTCCT 20 20 45.0 50.0 57.9 58.0 31.5 0.0 0.135901 118391 118715 1131-1131 0.500000 58.9 ndhE-psaC_p5 ndhE-psaC 5 ACCGGCTATTGTTTCAGCAA GCCAAGCCGATAAAGTAGCT 20 20 45.0 50.0 58.1 58.0 0.0 0.0 0.140786 118391 118715 1082-1082 0.500000 58.9 psaC-ndhD_p1 psaC-ndhD 1 AACAGAAGACTGCGTTGGTT TCTTTCCTCCCCACATGGAT 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.052765 118962 119431 709-709 0.500000 59.6 psaC-ndhD_p2 psaC-ndhD 2 AACAGAAGACTGCGTTGGTT TAATCGGCGATTTGACAGCA 20 20 45.0 45.0 58.0 58.0 0.0 36.3 0.055525 118962 119431 916-916 0.500000 59.6 psaC-ndhD_p3 psaC-ndhD 3 AACAGAAGACTGCGTTGGTT ACCCATACACAGAACTCCCA 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.077250 118962 119431 792-792 0.500000 59.4 psaC-ndhD_p4 psaC-ndhD 4 AACAGAAGACTGCGTTGGTT ATACGAACCAACCCATACGC 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.094355 118962 119431 1025-1025 0.500000 59.2 psaC-ndhD_p5 psaC-ndhD 5 AACAGAAGACTGCGTTGGTT GCCGAGTACAGACGTTTCTT 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.100127 118962 119431 725-725 0.500000 59.2 ndhD_p1 ndhD 1 AGGAGCACTGGTTTTTCTGG GTGAACCGACACGAATCGTA 20 20 50.0 50.0 58.0 58.0 0.0 8.4 0.045356 119432 120595 1720-1720 0.500000 59.6 ndhD_p2 ndhD 2 AACAGAAGACTGCGTTGGTT GTGAACCGACACGAATCGTA 20 20 45.0 50.0 58.0 58.0 0.0 8.4 0.070625 119432 120595 1911-1911 0.500000 59.4 ndhD_p3 ndhD 3 AGCTACTTTATCGGCTTGGC GTGAACCGACACGAATCGTA 20 20 50.0 50.0 58.0 58.0 0.0 8.4 0.077311 119432 120595 1381-1381 0.500000 59.4 ndhD_p4 ndhD 4 AACAAATCGCTTCTGCTCCA GTGAACCGACACGAATCGTA 20 20 45.0 50.0 58.1 58.0 0.0 8.4 0.136019 119432 120595 1933-1933 0.500000 58.9 ndhD_p5 ndhD 5 ACAAATCGCTTCTGCTCCAA GTGAACCGACACGAATCGTA 20 20 45.0 50.0 58.1 58.0 0.0 8.4 0.136019 119432 120595 1932-1932 0.500000 58.9 ccsA_p1 ccsA 1 ATGAGTGGCTTTGTTGCTGA AAGTTCAACACAAGCCGCTA 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.052733 120812 121804 1733-1733 0.500000 59.6 ccsA_p2 ccsA 2 ATGAGTGGCTTTGTTGCTGA TAAGTTCAACACAAGCCGCT 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.052733 120812 121804 1734-1734 0.500000 59.6 ccsA_p3 ccsA 3 TACGATTCGTGTCGGTTCAC AAGTTCAACACAAGCCGCTA 20 20 50.0 45.0 58.0 58.0 21.4 0.0 0.070445 120812 121804 1246-1246 0.500000 59.4 ccsA_p4 ccsA 4 TACGATTCGTGTCGGTTCAC TAAGTTCAACACAAGCCGCT 20 20 50.0 45.0 58.0 58.0 21.4 0.0 0.070445 120812 121804 1247-1247 0.500000 59.4 ccsA_p5 ccsA 5 GCTTGCATTACCGGGTATGA AAGTTCAACACAAGCCGCTA 20 20 50.0 45.0 58.0 58.0 18.9 0.0 0.076413 120812 121804 1749-1749 0.500000 59.4 trnL-UAG-ndhF_p1 trnL-UAG-ndhF 1 TAGCTAGCAGGCCTTTTTCG CAAGAGGTCGTGTGAACCAA 20 20 50.0 50.0 58.0 58.1 18.8 0.0 0.076071 121971 123855 2716-2716 0.500000 59.4 trnL-UAG-ndhF_p2 trnL-UAG-ndhF 2 TCGAACCGAGATGCTTAAGC CAAGAGGTCGTGTGAACCAA 20 20 50.0 50.0 58.1 58.1 20.8 0.0 0.129325 121971 123855 2461-2461 0.500000 59.0 trnL-UAG-ndhF_p3 trnL-UAG-ndhF 3 AACCACTCGGATTGAAGAGC CAAGAGGTCGTGTGAACCAA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.152500 121971 123855 2981-2981 0.500000 58.8 trnL-UAG-ndhF_p4 trnL-UAG-ndhF 4 AAGCACTGCTTCCTAAGAGC CAAGAGGTCGTGTGAACCAA 20 20 50.0 50.0 57.9 58.1 10.7 0.0 0.158373 121971 123855 2445-2445 0.500000 58.7 trnL-UAG-ndhF_p5 trnL-UAG-ndhF 5 TCCTAAGAGCAGCGTGTCTA CAAGAGGTCGTGTGAACCAA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.176173 121971 123855 2435-2435 0.500000 58.6 ndhF_p1 ndhF 1 GCGCATATCTTCCCTTTTGC GCAGAATACCGTTACCCGTT 20 20 50.0 50.0 57.9 58.0 0.3 0.0 0.139797 123856 126093 2579-2579 0.500000 58.9 ndhF_p2 ndhF 2 GCGCATATCTTCCCTTTTGC ATCCGGGCAAAATCGATGAA 20 20 50.0 45.0 57.9 57.9 0.3 0.0 0.176796 123856 126093 2501-2501 0.500000 58.6 ndhF_p3 ndhF 3 GCGCATATCTTCCCTTTTGC CCGGGCAAAATCGATGAAAC 20 20 50.0 50.0 57.9 58.1 0.3 0.0 0.217396 123856 126093 2499-2499 0.500000 58.3 ndhF_p4 ndhF 4 GCGCATATCTTCCCTTTTGC GGGCAAAATCGATGAAACGG 20 20 50.0 50.0 57.9 58.1 0.3 0.0 0.217396 123856 126093 2497-2497 0.500000 58.3 ndhF_p5 ndhF 5 GCGCATATCTTCCCTTTTGC TCCGGGCAAAATCGATGAAA 20 20 50.0 45.0 57.9 58.2 0.3 0.0 0.297780 123856 126093 2500-2500 0.500000 57.6