primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	TCCTATATGTTAGCAGAAAAACC	TATGTGTGCTTGGGAGTCCT	23	20	34.8	50.0	54.2	58.3	0.0	9.6	7.170784	87	1148	1133-1133	1.000000	40.0
psbA_p2	psbA	2	TTCCTATATGTTAGCAGAAAAACC	TATGTGTGCTTGGGAGTCCT	24	20	33.3	50.0	54.9	58.3	0.0	9.6	7.415053	87	1148	1134-1134	1.000000	40.0
psbA_p3	psbA	3	TCCTATATGTTAGCAGAAAAACC	TGGGAGTCCTTGCAATTTGA	23	20	34.8	45.0	54.2	57.3	0.0	9.6	7.501147	87	1148	1123-1123	1.000000	40.0
psbA_p4	psbA	4	TCCTATATGTTAGCAGAAAAACC	ATATGTGTGCTTGGGAGTCC	23	20	34.8	50.0	54.2	57.3	0.0	0.0	7.545011	87	1148	1134-1134	1.000000	40.0
psbA_p5	psbA	5	TCCTATATGTTAGCAGAAAAACC	GCTTGGGAGTCCTTGCAATT	23	20	34.8	50.0	54.2	58.7	0.0	9.6	7.566828	87	1148	1126-1126	1.000000	40.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5652	6433	856-887	1.000000	78.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5652	6433	867-898	1.000000	78.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5652	6433	860-891	1.000000	77.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5652	6433	871-902	1.000000	77.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5652	6433	862-893	1.000000	75.9
petN-trnC-GCA_p1	petN-trnC-GCA	1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	20	19	50.0	52.6	58.2	58.1	0.0	0.0	1.204402	17052	17949	947-965	1.000000	70.5
petN-trnC-GCA_p2	petN-trnC-GCA	2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	20	20	50.0	55.0	58.2	59.1	0.0	4.3	1.260665	17052	17949	949-967	1.000000	70.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	20	19	60.0	52.6	58.3	58.1	0.0	0.0	1.334774	17052	17949	1003-1021	1.000000	69.5
petN-trnC-GCA_p4	petN-trnC-GCA	4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	20	20	60.0	55.0	58.3	59.1	0.0	4.3	1.391037	17052	17949	1005-1023	1.000000	69.0
petN-trnC-GCA_p5	petN-trnC-GCA	5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	20	19	55.0	52.6	58.4	58.1	0.0	0.0	1.425206	17052	17949	1004-1022	1.000000	68.8
atpI-atpH_p1	atpI-atpH	1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31097	31665	659-659	1.000000	79.0
atpI-atpH_p2	atpI-atpH	2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31097	31665	662-662	1.000000	79.0
atpI-atpH_p3	atpI-atpH	3	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	20	20	45.0	55.0	57.9	58.2	0.0	0.0	0.253472	31097	31665	716-716	1.000000	78.2
atpI-atpH_p4	atpI-atpH	4	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	20	20	45.0	45.0	57.9	57.8	0.0	3.2	0.268178	31097	31665	649-649	1.000000	78.1
atpI-atpH_p5	atpI-atpH	5	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.277543	31097	31665	671-671	1.000000	78.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.254079	43519	44114	729-729	1.000000	78.2
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.322349	43519	44114	731-731	1.000000	77.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTCAA	20	20	50.0	45.0	58.3	57.8	0.0	0.0	0.480608	43519	44114	730-730	1.000000	76.4
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAGGTTGTCGGA	TTCCAATGCTACGCCTTCAA	20	20	50.0	45.0	58.3	57.8	0.0	0.0	0.480608	43519	44114	731-731	1.000000	76.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TGAAATTTCCATTTGCGCGG	TTCCAATGCTACGCCTTCAA	20	20	45.0	45.0	58.3	57.8	15.5	0.0	0.485032	43519	44114	665-665	1.000000	76.4
ndhJ_p1	ndhJ	1	CGTTTCATCTAGGAACAGAACG	TCTTGCATCCGAAATGTGAA	22	20	45.5	40.0	57.2	55.7	0.0	0.0	5.164808	47920	48399	622-622	1.000000	41.2
ndhJ_p2	ndhJ	2	CGTTTCATCTAGGAACAGAACG	TTCTTGCATCCGAAATGTGA	22	20	45.5	40.0	57.2	55.7	0.0	0.0	5.164808	47920	48399	623-623	1.000000	41.2
ndhJ_p3	ndhJ	3	TCCGTTTCATCTAGGAACAGA	TCTTGCATCCGAAATGTGAA	21	20	42.9	40.0	56.1	55.7	0.0	0.0	5.227626	47920	48399	624-624	1.000000	41.2
ndhJ_p4	ndhJ	4	TCCGTTTCATCTAGGAACAGA	TTCTTGCATCCGAAATGTGA	21	20	42.9	40.0	56.1	55.7	0.0	0.0	5.227626	47920	48399	625-625	1.000000	41.2
ndhJ_p5	ndhJ	5	CGTTTCATCTAGGAACAGAACG	CTTTCTTGCATCCGAAATGTGA	22	22	45.5	40.9	57.2	57.6	0.0	6.8	5.248909	47920	48399	625-625	1.000000	41.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCTGAAATCCAAAATGCC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.104399	49593	50486	953-1004	1.000000	83.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCCCTGAAATCCAAAATGCC	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.0	58.3	0.0	22.8	0.333780	49593	50486	954-1005	1.000000	81.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCAGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.3	57.9	0.0	0.0	0.732539	49593	50486	982-1033	1.000000	78.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCAGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.812623	49593	50486	981-1032	1.000000	77.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.2	57.9	6.3	0.0	0.849972	49593	50486	972-1023	1.000000	77.4
atpB-rbcL_p1	atpB-rbcL	1	AAAGTGACATCTAGCACGGG	CCTTGGTTTCATACTCCGGG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.425048	53446	54238	994-994	1.000000	76.7
atpB-rbcL_p2	atpB-rbcL	2	AAAGTGACATCTAGCACGGG	CTTGGTTTCATACTCCGGGG	20	20	50.0	55.0	57.6	58.0	0.0	0.0	0.425048	53446	54238	993-993	1.000000	76.7
atpB-rbcL_p3	atpB-rbcL	3	AAAGTGACATCTAGCACGGG	ACTCCGGGGTGTAGTAAGTC	20	20	50.0	55.0	57.6	58.2	0.0	5.9	0.541974	53446	54238	982-982	1.000000	75.7
atpB-rbcL_p4	atpB-rbcL	4	AAAGTGACATCTAGCACGGG	CTCCGGGGTGTAGTAAGTCA	20	20	50.0	55.0	57.6	58.2	0.0	0.0	0.543292	53446	54238	981-981	1.000000	75.7
atpB-rbcL_p5	atpB-rbcL	5	AAAGTGACATCTAGCACGGG	TCCGGGGTGTAGTAAGTCAA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.694157	53446	54238	980-980	1.000000	74.5
rbcL-psaI_p1	rbcL-psaI	1	AGTTCGAGCCGGTAGATACT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.7	58.0	1.9	26.9	0.372128	55673	56854	1284-1285	1.000000	78.0
rbcL-psaI_p2	rbcL-psaI	2	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	55673	56854	1298-1299	1.000000	77.9
rbcL-psaI_p3	rbcL-psaI	3	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	55673	56854	1303-1304	1.000000	77.6
rbcL-psaI_p4	rbcL-psaI	4	AGTTCGAGCCGGTAGATACT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.7	57.9	1.9	26.8	0.448661	55673	56854	1290-1291	1.000000	77.4
rbcL-psaI_p5	rbcL-psaI	5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.456795	55673	56854	1304-1305	1.000000	77.3
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCATATTCTGGATTGGG	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.231322	60169	60956	925-932	1.000000	62.3
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	CGGTTCATATTCTGGATTGGGT	20	22	50.0	45.5	57.9	58.2	0.0	0.0	2.231322	60169	60956	924-931	1.000000	62.3
petA-psbJ_p3	petA-psbJ	3	TGTTCTTCTTCGCATCCGTT	ACGGTTCATATTCTGGATTGGG	20	22	45.0	45.5	57.8	58.2	0.0	0.0	2.343261	60169	60956	918-925	1.000000	61.4
petA-psbJ_p4	petA-psbJ	4	TGTTCTTCTTCGCATCCGTT	CGGTTCATATTCTGGATTGGGT	20	22	45.0	45.5	57.8	58.2	0.0	0.0	2.343261	60169	60956	917-924	1.000000	61.4
petA-psbJ_p5	petA-psbJ	5	TTGTTCTTCTTCGCATCCGT	CGGTTCATATTCTGGATTGGGT	20	22	45.0	45.5	57.8	58.2	0.0	0.0	2.343261	60169	60956	918-925	1.000000	61.4
clpP-psbB_p1	clpP-psbB	1	ACCCAAGTCGCTTCTTCATC	TATAAAGCCATCGAGCCAGC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.270437	67811	68347	718-718	1.000000	78.1
clpP-psbB_p2	clpP-psbB	2	ACCCAAGTCGCTTCTTCATC	CCAGCCCAACCAGAAACTAG	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.274220	67811	68347	703-703	1.000000	78.0
clpP-psbB_p3	clpP-psbB	3	TCAACCCAAGTCGCTTCTTC	TATAAAGCCATCGAGCCAGC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.290879	67811	68347	721-721	1.000000	77.9
clpP-psbB_p4	clpP-psbB	4	TCAACCCAAGTCGCTTCTTC	CCAGCCCAACCAGAAACTAG	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.294662	67811	68347	706-706	1.000000	77.9
clpP-psbB_p5	clpP-psbB	5	ACCCAAGTCGCTTCTTCATC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.337897	67811	68347	619-619	1.000000	77.6
psbB_p1	psbB	1	TGGATAGTCTTTTCCAATGCGA	GGGAGCTGTTTTGTTTCCCA	22	20	40.9	50.0	57.8	58.6	2.5	0.0	2.806017	68348	69874	1664-1664	1.000000	57.7
psbB_p2	psbB	2	TGGATAGTCTTTTCCAATGCGA	TGGGAGCTGTTTTGTTTCCC	22	20	40.9	50.0	57.8	58.6	2.5	0.0	2.806017	68348	69874	1665-1665	1.000000	57.7
psbB_p3	psbB	3	TGGATAGTCTTTTCCAATGCGA	AAGGATGGGAGCTGTTTTGT	22	20	40.9	45.0	57.8	57.3	2.5	0.0	2.898197	68348	69874	1670-1670	1.000000	57.0
psbB_p4	psbB	4	TGGATAGTCTTTTCCAATGCGA	AGGATGGGAGCTGTTTTGTT	22	20	40.9	45.0	57.8	57.3	2.5	0.0	2.898197	68348	69874	1669-1669	1.000000	57.0
psbB_p5	psbB	5	TGGATAGTCTTTTCCAATGCGA	GGAGCTGTTTTGTTTCCCATG	22	21	40.9	47.6	57.8	58.0	2.5	0.0	3.245654	68348	69874	1663-1663	1.000000	54.2
rps11-rpl36_p1	rps11-rpl36	1	GCGCACTCTTCCGTAAACTA	AGCATAAACAAAGACAGGGGT	20	21	50.0	42.9	58.0	57.5	0.3	0.0	1.539102	75271	75462	306-307	1.000000	68.5
rps11-rpl36_p2	rps11-rpl36	2	TATTCTACGCGCACTCTTCC	AGCATAAACAAAGACAGGGGT	20	21	50.0	42.9	57.5	57.5	5.7	0.0	2.012751	75271	75462	314-315	1.000000	64.7
rps11-rpl36_p3	rps11-rpl36	3	GCGCACTCTTCCGTAAACTA	GCATAAACAAAGACAGGGGT	20	20	50.0	45.0	58.0	55.9	0.3	0.0	2.094793	75271	75462	305-306	1.000000	64.0
rps11-rpl36_p4	rps11-rpl36	4	GCGCACTCTTCCGTAAACTA	CCGAAGCATAAACAAAGACAGG	20	22	50.0	45.5	58.0	57.9	0.3	0.0	2.096883	75271	75462	310-311	1.000000	64.0
rps11-rpl36_p5	rps11-rpl36	5	GCGCACTCTTCCGTAAACTA	CGAAGCATAAACAAAGACAGGG	20	22	50.0	45.5	58.0	57.9	0.3	0.0	2.096883	75271	75462	309-310	1.000000	64.0
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TGCTAAAGAATAAGCCTGTACT	20	22	55.0	36.4	58.1	54.9	0.0	0.0	5.209782	103597	104366	857-925	1.000000	44.3
ndhF-rpl32_p2	ndhF-rpl32	2	GGCCAAACCCCATTGACATA	TGCTAAAGAATAAGCCTGTACT	20	22	50.0	36.4	58.1	54.9	0.0	0.0	5.236903	103597	104366	883-951	1.000000	44.3
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGGCCAAACCCCATTGA	TGCTAAAGAATAAGCCTGTACT	20	22	45.0	36.4	58.2	54.9	5.0	0.0	5.283102	103597	104366	887-955	1.000000	44.3
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTGCTAAAGAATAAGCCTGTACT	20	23	55.0	34.8	58.1	55.7	0.0	0.0	5.448653	103597	104366	858-926	1.000000	44.3
ndhF-rpl32_p5	ndhF-rpl32	5	GGCCAAACCCCATTGACATA	TTGCTAAAGAATAAGCCTGTACT	20	23	50.0	34.8	58.1	55.7	0.0	0.0	5.475773	103597	104366	884-952	1.000000	44.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCAATGAGCATCCAAAACCA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.3	58.0	0.0	0.0	0.751105	104547	105202	775-781	1.000000	75.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCAATGAGCATCCAAAACCA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	57.3	58.1	0.0	0.0	0.806071	104547	105202	778-784	1.000000	75.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCAATGAGCATCCAAAACCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.871673	104547	105202	748-754	1.000000	74.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCAATGAGCATCCAAAACCA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.3	57.8	0.0	0.0	0.932400	104547	105202	741-747	1.000000	74.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	ATTTTCGAGTGGCAATGAGC	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.1	58.0	0.0	0.0	0.931262	104547	105202	786-792	1.000000	74.3
ndhI_p1	ndhI	1	AGGATTCCAGGAAGTAAAAAGGG	TCAACACAAAGGCTCTGTCA	23	20	43.5	45.0	58.1	57.3	8.2	0.0	3.772638	110003	110545	665-665	1.000000	50.1
ndhI_p2	ndhI	2	GGATTCCAGGAAGTAAAAAGGGA	TCAACACAAAGGCTCTGTCA	23	20	43.5	45.0	58.1	57.3	8.2	0.0	3.777520	110003	110545	664-664	1.000000	50.0
ndhI_p3	ndhI	3	GGATTCCAGGAAGTAAAAAGGG	TCAACACAAAGGCTCTGTCA	22	20	45.5	45.0	56.7	57.3	8.2	0.0	4.049002	110003	110545	664-664	1.000000	47.8
ndhI_p4	ndhI	4	AGGATTCCAGGAAGTAAAAAGGG	TCAACACAAAGGCTCTGTCAA	23	21	43.5	42.9	58.1	58.0	8.2	0.0	4.066441	110003	110545	665-665	1.000000	47.7
ndhI_p5	ndhI	5	AGGATTCCAGGAAGTAAAAAGGG	TTCAACACAAAGGCTCTGTCA	23	21	43.5	42.9	58.1	58.0	8.2	0.0	4.066441	110003	110545	666-666	1.000000	47.7