primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH (GUG)-psbA_p1	trnH (GUG)-psbA	1	CAATCCACTGCCTTCATCCA	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.469587	78	418	313-525	1.000000	88.2
trnH (GUG)-psbA_p2	trnH (GUG)-psbA	2	AATCCACTGCCTTCATCCAC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	418	312-524	1.000000	88.2
trnH (GUG)-psbA_p3	trnH (GUG)-psbA	3	CAATCCACTGCCTTCATCCA	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.529871	78	418	314-526	1.000000	87.8
trnH (GUG)-psbA_p4	trnH (GUG)-psbA	4	AATCCACTGCCTTCATCCAC	ACCGTGCTAACCTTGGTATG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531379	78	418	313-525	1.000000	87.7
trnH (GUG)-psbA_p5	trnH (GUG)-psbA	5	ACAATCCACTGCCTTCATCC	CCGTGCTAACCTTGGTATGG	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.471094	78	418	314-526	0.977778	87.3
psbA-rbcL_p1	psbA-rbcL	1	TACAGAAGCGACCCCATAGG	ATTTGAGTTTGCCGCAATGG	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.673162	1481	2512	862-1177	1.000000	86.6
psbA-rbcL_p2	psbA-rbcL	2	TACAGAAGCGACCCCATAGG	GCTGCTTGTGAGGTATGGAA	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.703856	1481	2512	891-1206	1.000000	86.4
psbA-rbcL_p3	psbA-rbcL	3	TACAGAAGCGACCCCATAGG	TGAGTTTGCCGCAATGGATA	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.711287	1481	2512	859-1174	1.000000	86.3
psbA-rbcL_p4	psbA-rbcL	4	TACAGAAGCGACCCCATAGG	AGTTTGCCGCAATGGATACT	20	20	55.0	45.0	58.6	57.9	0.0	6.6	0.718766	1481	2512	857-1172	1.000000	86.2
psbA-rbcL_p5	psbA-rbcL	5	ATCCAGTTACAGAAGCGACC	ATTTGAGTTTGCCGCAATGG	20	20	50.0	45.0	57.3	57.9	0.0	6.6	0.757962	1481	2512	869-1184	1.000000	85.9
rbcL-atpB_p1	rbcL-atpB	1	ACACCAGCTTTGAATCCAACA	CAATGATTTGGCCGATACGC	21	20	42.9	50.0	58.3	58.0	0.0	0.0	1.371391	3941	4757	860-956	1.000000	81.0
rbcL-atpB_p2	rbcL-atpB	2	AGTCTCTGTTTGTGGTGACA	CAATGATTTGGCCGATACGC	20	20	45.0	50.0	56.6	58.0	0.0	0.0	1.408253	3941	4757	831-927	1.000000	80.7
rbcL-atpB_p3	rbcL-atpB	3	ACACCAGCTTTGAATCCAACA	CCCCAGAACCAGAAGGAGTA	21	20	42.9	55.0	58.3	58.0	0.0	0.0	1.382213	3941	4757	812-908	0.977778	80.1
rbcL-atpB_p4	rbcL-atpB	4	ACACCAGCTTTGAATCCAACA	AGCTACATCTAGGACCGGAC	21	20	42.9	55.0	58.3	58.0	0.0	9.5	1.350769	3941	4757	880-976	0.911111	77.6
rbcL-atpB_p5	rbcL-atpB	5	AGTCTCTGTTTGTGGTGACA	AGCTACATCTAGGACCGGAC	20	20	45.0	55.0	56.6	58.0	0.0	9.5	1.387630	3941	4757	851-947	0.911111	77.3
trnK (UUU)-rps16_p1	trnK (UUU)-rps16	1	AAAGCCGAGTACTCTACCGT	AACGAACTTTGCCCCAATCA	20	20	50.0	45.0	58.2	58.3	20.3	0.0	0.469914	10738	11224	548-672	0.866667	82.9
trnK (UUU)-rps16_p2	trnK (UUU)-rps16	2	AAAGCCGAGTACTCTACCGT	ACGAACTTTGCCCCAATCAA	20	20	50.0	45.0	58.2	58.3	20.3	0.0	0.469914	10738	11224	547-671	0.866667	82.9
trnK (UUU)-rps16_p3	trnK (UUU)-rps16	3	AAGCCGAGTACTCTACCGTT	AACGAACTTTGCCCCAATCA	20	20	50.0	45.0	58.2	58.3	20.3	0.0	0.469914	10738	11224	547-671	0.866667	82.9
trnK (UUU)-rps16_p4	trnK (UUU)-rps16	4	AAGCCGAGTACTCTACCGTT	ACGAACTTTGCCCCAATCAA	20	20	50.0	45.0	58.2	58.3	20.3	0.0	0.469914	10738	11224	546-670	0.866667	82.9
trnK (UUU)-rps16_p5	trnK (UUU)-rps16	5	TCAGTCGTGGTCTTCCAAAC	ACGAACTTTGCCCCAATCAA	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.535794	10738	11224	632-780	0.866667	82.4
ORF198_p1	ORF198	1	TATCAAAGCGCATCCAACGA	TTGTCGCTAGAGATTTGGGC	20	20	45.0	50.0	58.0	58.0	11.5	0.0	0.041314	11665	12261	1664-2387	0.533333	73.0
ORF198_p2	ORF198	2	AGCGAACATCAATTGCAACG	TTGTCGCTAGAGATTTGGGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.056414	11665	12261	1177-1821	0.511111	72.0
ORF198_p3	ORF198	3	CGCATCCAACGAACTCCTTA	TTGTCGCTAGAGATTTGGGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028758	11665	12261	1656-2379	0.488889	71.3
ORF198_p4	ORF198	4	CGCATCCAACGAACTCCTTA	TGACACGGGCAGTTGTAAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.044401	11665	12261	1572-1622	0.244444	54.7
ORF198_p5	ORF198	5	TATCAAAGCGCATCCAACGA	TGACACGGGCAGTTGTAAAA	20	20	45.0	45.0	58.0	58.0	11.5	0.0	0.056956	11665	12261	1580-1630	0.244444	54.6
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.0	58.3	1.4	0.0	0.297359	19219	20013	918-959	1.000000	89.6
atpH-atpI_p2	atpH-atpI	2	AGCCAATCCAGCAGCAATAA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.407418	19219	20013	934-975	1.000000	88.7
atpH-atpI_p3	atpH-atpI	3	AATAACGGAAGCGGCAGAAA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.2	58.3	1.4	0.0	0.442898	19219	20013	919-960	1.000000	88.5
atpH-atpI_p4	atpH-atpI	4	GCCAATCCAGCAGCAATAAC	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.473359	19219	20013	933-974	1.000000	88.2
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	TCCCTGTCATGTTCCTTGGA	20	20	50.0	50.0	58.3	58.3	0.0	0.0	0.521134	19219	20013	928-969	1.000000	87.8
ORF304_p1	ORF304	1	ACGAGTCCTGCGGATACATA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041687	32803	33717	2079-2583	0.888889	87.2
ORF304_p2	ORF304	2	CCTGATCGATGAACCTGCAA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.5	0.0	0.047163	32803	33717	1907-2841	0.822222	84.5
ORF304_p3	ORF304	3	AACGTTACCGGCAATCTACC	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033079	32803	33717	1571-2006	0.755556	82.0
ORF304_p4	ORF304	4	AACGTTACCGGCAATCTACC	CTCACCGAAGACTGTTGAGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.086024	32803	33717	1549-1984	0.755556	81.5
ORF304_p5	ORF304	5	AACGTTACCGGCAATCTACC	CACCTATTACCGCACCTGAC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.078403	32803	33717	1226-1336	0.355556	63.6
ORF304-trnC (GCA)_p1	ORF304-trnC (GCA)	1	ATAGAGTCCAACCCCAACGA	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071759	33718	34183	768-915	0.822222	84.3
ORF304-trnC (GCA)_p2	ORF304-trnC (GCA)	2	TACCCTCGCGACAAGATAGT	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	3.7	0.0	0.067188	33718	34183	1440-1602	0.733333	80.8
ORF304-trnC (GCA)_p3	ORF304-trnC (GCA)	3	TGTCCTTGTTATCCGAGGGT	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071759	33718	34183	924-1054	0.577778	74.4
ORF304-trnC (GCA)_p4	ORF304-trnC (GCA)	4	TTCTTTACCCTCGCGACAAG	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	57.9	58.0	3.7	0.0	0.093290	33718	34183	1465-1597	0.288889	58.9
ORF304-trnC (GCA)_p5	ORF304-trnC (GCA)	5	TTTCGGTAGATGCAGAAGCC	GGTGCAGAGTCAAAAAGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.042919	33718	34183	880-905	0.066667	45.7
psbM-trnD (GUC)_p1	psbM-trnD (GUC)	1	TGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	20	20	40.0	55.0	54.3	57.8	0.0	6.3	3.876263	35204	36216	898-1138	0.977778	60.1
psbM-trnD (GUC)_p2	psbM-trnD (GUC)	2	TGCCAGAATATTGACTTCCA	TTCAATTGGTCAGAGCACCG	20	20	40.0	50.0	54.3	58.5	0.0	5.4	4.155261	35204	36216	871-1111	1.000000	58.8
psbM-trnD (GUC)_p3	psbM-trnD (GUC)	3	ATGCCAGAATATTGACTTCCA	AGATCCCTTAGTTCCCTGGG	21	20	38.1	55.0	55.0	57.8	0.0	6.3	4.243175	35204	36216	899-1139	0.977778	57.2
psbM-trnD (GUC)_p4	psbM-trnD (GUC)	4	TGCCAGAATATTGACTTCCAT	AGATCCCTTAGTTCCCTGGG	21	20	38.1	55.0	55.0	57.8	0.0	6.3	4.243175	35204	36216	898-1138	0.977778	57.2
psbM-trnD (GUC)_p5	psbM-trnD (GUC)	5	GGAACTAAAATGAAGAGTGCAGT	AGATCCCTTAGTTCCCTGGG	23	20	39.1	55.0	57.1	57.8	0.0	6.3	4.147326	35204	36216	930-1170	0.955556	57.0
ndhC-ORF227_p1	ndhC-ORF227	1	CTAGAAAGTTTCTCCGGCCC	CAATATCCAAGCTCCGCAGT	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.066547	56165	56617	906-1227	0.866667	86.1
ndhC-ORF227_p2	ndhC-ORF227	2	CTAGAAAGTTTCTCCGGCCC	TTTTCTTGGTGGAAGGCCTC	20	20	55.0	50.0	58.0	58.0	21.2	5.2	0.030212	56165	56617	1145-1235	0.844444	85.5
ndhC-ORF227_p3	ndhC-ORF227	3	TCTCACTAAAGAAGGGGCCA	TTTTCTTGGTGGAAGGCCTC	20	20	50.0	50.0	58.0	58.0	4.4	5.2	0.043487	56165	56617	1703-1793	0.844444	85.4
ndhC-ORF227_p4	ndhC-ORF227	4	CTAGAAAGTTTCTCCGGCCC	CAAGACCCCCGTTTAACACA	20	20	55.0	50.0	58.0	58.0	21.2	0.0	0.055497	56165	56617	515-833	0.777778	82.7
ndhC-ORF227_p5	ndhC-ORF227	5	CTAGAAAGTTTCTCCGGCCC	ATCGATTAAGTTGCCGCCTT	20	20	55.0	45.0	58.0	58.0	21.2	0.0	0.063975	56165	56617	1050-1093	0.755556	81.2
ORF227_p1	ORF227	1	AAAGCAGAAGCATAGACGCA	TTTTCGACGGAAGCTGCTTA	20	20	45.0	45.0	57.9	57.8	0.0	16.0	0.256975	56618	57301	1166-1636	0.911111	86.4
ORF227_p2	ORF227	2	CTAGAAAGTTTCTCCGGCCC	TTTTCGACGGAAGCTGCTTA	20	20	55.0	45.0	58.0	57.8	21.2	16.0	0.183423	56618	57301	1281-1751	0.888889	86.1
ORF227_p3	ORF227	3	CTAGAAAGTTTCTCCGGCCC	TCCTTCGTACGACCCCATTA	20	20	55.0	50.0	58.0	57.9	21.2	19.4	0.167945	56618	57301	1654-1681	0.355556	56.3
ORF227_p4	ORF227	4	AAAGCAGAAGCATAGACGCA	TCCTTCGTACGACCCCATTA	20	20	45.0	50.0	57.9	57.9	0.0	19.4	0.241497	56618	57301	1539-1566	0.355556	55.8
ORF227_p5	ORF227	5	TGCTAATGGGGGCTAAGACT	TCCTTCGTACGACCCCATTA	20	20	50.0	50.0	58.1	57.9	0.0	19.4	0.249041	56618	57301	1632-1636	0.333333	54.0
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	62722	63653	677-1355	1.000000	90.7
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	62722	63653	679-1357	1.000000	90.7
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	62722	63653	763-1441	1.000000	90.6
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	AAGCCGCTAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.176991	62722	63653	765-1443	1.000000	90.6
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.184355	62722	63653	1060-1446	0.955556	88.7
rpl16-rps3_p1	rpl16-rps3	1	TGCGAAGTGATGAATTGGGT	CGAGAAGGTAGAGTTCCCCT	20	20	45.0	55.0	57.8	57.9	0.0	0.0	0.345747	80602	80766	376-399	0.822222	82.1
rpl16-rps3_p2	rpl16-rps3	2	AACGAGTCACACACTAAGCA	CGAGAAGGTAGAGTTCCCCT	20	20	45.0	55.0	57.1	57.9	0.0	0.0	1.029847	80602	80766	296-318	0.911111	80.2
rpl16-rps3_p3	rpl16-rps3	3	TGCGAAGTGATGAATTGGGT	CCCTACAAACCATTCAAGCG	20	20	45.0	50.0	57.8	57.1	0.0	0.0	1.118493	80602	80766	360-383	0.888889	78.6
rpl16-rps3_p4	rpl16-rps3	4	ACGAGTCACACACTAAGCAT	CGAGAAGGTAGAGTTCCCCT	20	20	45.0	55.0	56.9	57.9	0.0	0.0	1.256135	80602	80766	295-317	0.911111	78.4
rpl16-rps3_p5	rpl16-rps3	5	CAACGAGTCACACACTAAGC	CGAGAAGGTAGAGTTCCCCT	20	20	50.0	55.0	56.8	57.9	0.0	0.0	1.357060	80602	80766	297-319	0.911111	77.6
trnL (UAG)-rpl32_p1	trnL (UAG)-rpl32	1	AGCGTGTCTACCGATTTCAC	ACATGGAAATGCAGTGTCGA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.191308	122680	123395	1038-1303	0.844444	84.2
trnL (UAG)-rpl32_p2	trnL (UAG)-rpl32	2	CAGCGTGTCTACCGATTTCA	ACATGGAAATGCAGTGTCGA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.192679	122680	123395	1039-1304	0.844444	84.2
trnL (UAG)-rpl32_p3	trnL (UAG)-rpl32	3	AAAAGGTCGGTATCATGCCC	ACATGGAAATGCAGTGTCGA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.236260	122680	123395	1547-1609	0.755556	80.3
trnL (UAG)-rpl32_p4	trnL (UAG)-rpl32	4	GGTCGGTATCATGCCCTTTT	ACATGGAAATGCAGTGTCGA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.236260	122680	123395	1543-1605	0.755556	80.3
trnL (UAG)-rpl32_p5	trnL (UAG)-rpl32	5	AAAGACTAGAAGTGCAGCGG	ACATGGAAATGCAGTGTCGA	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.269532	122680	123395	1875-1936	0.755556	80.1
rpl32-ndhF_p1	rpl32-ndhF	1	CGCCGATGAATATCCCTTCC	GTTGCCGACGGAAAAAGAAG	20	20	55.0	50.0	58.3	58.0	0.0	20.5	0.305561	123561	124635	995-1359	0.933333	86.9
rpl32-ndhF_p2	rpl32-ndhF	2	CGCCGATGAATATCCCTTCC	AGAAGTCCCACCCCGATTAA	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.312014	123561	124635	977-1341	0.933333	86.8
rpl32-ndhF_p3	rpl32-ndhF	3	CGCCGATGAATATCCCTTCC	TGCCGACGGAAAAAGAAGAA	20	20	55.0	45.0	58.3	58.0	0.0	20.5	0.316850	123561	124635	993-1357	0.933333	86.8
rpl32-ndhF_p4	rpl32-ndhF	4	CGCCGATGAATATCCCTTCC	AAGAAGTCCCACCCCGATTA	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.312014	123561	124635	990-1342	0.911111	85.9
rpl32-ndhF_p5	rpl32-ndhF	5	CGCCGATGAATATCCCTTCC	GCAGATTTCGAAACAGCACC	20	20	55.0	50.0	58.3	58.0	0.0	13.8	0.304043	123561	124635	1622-1986	0.866667	84.2