primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	81	295	255-288	1.000000	88.3
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	81	295	305-338	1.000000	87.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	81	295	319-352	1.000000	87.4
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	81	295	318-351	1.000000	87.4
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	81	295	320-353	1.000000	87.4
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.3	57.9	0.0	0.0	0.434234	46671	47037	505-532	1.000000	83.0
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.7	57.9	0.0	0.0	0.439081	46671	47037	475-502	1.000000	83.0
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	58.3	57.8	0.0	0.0	0.541987	46671	47037	473-500	1.000000	82.3
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	GTTAGTCCCGGTAAAGCTCC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.7	57.8	0.0	0.0	0.546835	46671	47037	443-470	1.000000	82.3
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	TTTTTGAAACGAGGTCCCCG	CTCAGAGGTTAGAGCATCGC	20	20	50.0	55.0	58.7	57.9	0.0	0.0	0.821836	46671	47037	442-469	1.000000	80.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.230316	51033	52119	1227-1262	1.000000	81.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	0.0	0.0	0.715163	51033	52119	1214-1249	1.000000	77.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.806132	51033	52119	1212-1247	1.000000	76.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.828958	51033	52119	1222-1257	1.000000	76.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.8	58.1	5.2	0.0	0.936224	51033	52119	1209-1244	1.000000	75.6
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	63095	64020	979-1138	1.000000	75.4
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	63095	64020	966-1125	1.000000	75.0
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	63095	64020	980-1139	1.000000	74.7
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	63095	64020	967-1126	1.000000	74.3
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	63095	64020	984-1143	1.000000	73.8
ycf2-ndhB_p1	ycf2-ndhB	1	CAGCCCTTTGTCTCTGTGTT	ATCACGGGTTCTTCTCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088543	92479	93895	1729-2033	0.214286	54.0
ycf2-ndhB_p2	ycf2-ndhB	2	CAGCCCTTTGTCTCTGTGTT	TCACGGGTTCTTCTCTCCAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.088543	92479	93895	1728-2032	0.214286	54.0
ycf2-ndhB_p3	ycf2-ndhB	3	GTTCTTGCAGAGTGGAACCA	ATCACGGGTTCTTCTCTCCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.094097	92479	93895	1853-2157	0.214286	53.9
ycf2-ndhB_p4	ycf2-ndhB	4	GTTCTTGCAGAGTGGAACCA	TCACGGGTTCTTCTCTCCAT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.094097	92479	93895	1852-2156	0.214286	53.9
ycf2-ndhB_p5	ycf2-ndhB	5	GGCAAGCGGATCCTTTATGA	TCACGGGTTCTTCTCTCCAT	20	20	50.0	50.0	58.0	58.1	10.8	0.0	0.094097	92479	93895	1905-2209	0.214286	53.8
ndhB_p1	ndhB	1	GTTGCGGAAGAACGGACTAT	AAGCCCAAAGAGGTGAATGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.077102	93896	96110	2736-2874	0.214286	51.9
ndhB_p2	ndhB	2	GTTGCGGAAGAACGGACTAT	ATATAGGCCCGAGTTGGACA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.103081	93896	96110	2855-2993	0.214286	51.5
ndhB_p3	ndhB	3	GTTGCGGAAGAACGGACTAT	TATAGGCCCGAGTTGGACAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.103081	93896	96110	2854-2992	0.214286	51.5
ndhB_p4	ndhB	4	TAGCCGGAAAGTTATGTGGC	AAGCCCAAAGAGGTGAATGG	20	20	50.0	50.0	58.0	58.1	5.0	0.0	0.103812	93896	96110	2766-2766	0.142857	44.9
ndhB_p5	ndhB	5	TAGCCGGAAAGTTATGTGGC	TATAGGCCCGAGTTGGACAT	20	20	50.0	50.0	58.0	57.9	5.0	0.0	0.129790	93896	96110	2884-2884	0.142857	44.7
trnL-CAA-rps7_p1	trnL-CAA-rps7	1	AAATGGTAGACACGCGAGAC	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.029376	96765	97249	592-593	0.214286	48.6
trnL-CAA-rps7_p2	trnL-CAA-rps7	2	TGAAATGGTAGACACGCGAG	ATGGCAGAGGCAAATAGAGC	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.030746	96765	97249	594-595	0.214286	48.6
trnL-CAA-rps7_p3	trnL-CAA-rps7	3	AAATGGTAGACACGCGAGAC	TTGGTTATTAGCGGCATCCC	20	20	50.0	50.0	58.0	58.0	15.9	1.4	0.032171	96765	97249	716-717	0.214286	48.5
trnL-CAA-rps7_p4	trnL-CAA-rps7	4	TGAAATGGTAGACACGCGAG	TTGGTTATTAGCGGCATCCC	20	20	50.0	50.0	58.0	58.0	15.9	1.4	0.033541	96765	97249	718-719	0.214286	48.5
trnL-CAA-rps7_p5	trnL-CAA-rps7	5	AAATGGTAGACACGCGAGAC	TCCGAATTAGTGGATGCTGC	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.054271	96765	97249	658-659	0.214286	48.4
ycf1-ndhF_p1	ycf1-ndhF	1	CTCATGAAGGCCTACCGTTG	TGTTCGTTGGATCCCTAGGA	20	20	55.0	50.0	58.3	57.8	5.2	11.5	0.584628	111367	111413	637-708	1.000000	83.9
ycf1-ndhF_p2	ycf1-ndhF	2	CCCTGCCCACACTAGAAAAA	GTGGGCGCATCTCTTCTTAT	20	20	50.0	50.0	57.7	57.8	0.0	10.8	0.533083	111367	111413	838-909	1.000000	82.6
ycf1-ndhF_p3	ycf1-ndhF	3	CCCTGCCCACACTAGAAAAA	GGGGAATAAGAGGATTGGCC	20	20	50.0	55.0	57.7	57.7	0.0	0.0	0.580814	111367	111413	958-1029	1.000000	82.2
ycf1-ndhF_p4	ycf1-ndhF	4	CCCTGCCCACACTAGAAAAA	GGGGGAATAAGAGGATTGGC	20	20	50.0	55.0	57.7	57.7	0.0	0.0	0.580814	111367	111413	959-1030	1.000000	82.2
ycf1-ndhF_p5	ycf1-ndhF	5	CCCTGCCCACACTAGAAAAA	TGTTCGTTGGATCCCTAGGA	20	20	50.0	50.0	57.7	57.8	0.0	11.5	0.527645	111367	111413	1312-1383	1.000000	81.4
rps7-trnL-CAA_p1	rps7-trnL-CAA	1	ATGGCAGAGGCAAATAGAGC	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.029376	143163	143647	592-593	0.214286	48.6
rps7-trnL-CAA_p2	rps7-trnL-CAA	2	ATGGCAGAGGCAAATAGAGC	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.030746	143163	143647	594-595	0.214286	48.6
rps7-trnL-CAA_p3	rps7-trnL-CAA	3	TTGGTTATTAGCGGCATCCC	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	1.4	15.9	0.032171	143163	143647	716-717	0.214286	48.5
rps7-trnL-CAA_p4	rps7-trnL-CAA	4	TTGGTTATTAGCGGCATCCC	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	1.4	15.9	0.033541	143163	143647	718-719	0.214286	48.5
rps7-trnL-CAA_p5	rps7-trnL-CAA	5	TCCGAATTAGTGGATGCTGC	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.054271	143163	143647	658-659	0.214286	48.4
ndhB_p1	ndhB	1	AAGCCCAAAGAGGTGAATGG	GTTGCGGAAGAACGGACTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.077102	144302	146516	2736-2874	0.214286	51.9
ndhB_p2	ndhB	2	ATATAGGCCCGAGTTGGACA	GTTGCGGAAGAACGGACTAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103081	144302	146516	2855-2993	0.214286	51.5
ndhB_p3	ndhB	3	TATAGGCCCGAGTTGGACAT	GTTGCGGAAGAACGGACTAT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103081	144302	146516	2854-2992	0.214286	51.5
ndhB_p4	ndhB	4	AAGCCCAAAGAGGTGAATGG	TAGCCGGAAAGTTATGTGGC	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.103812	144302	146516	2766-2766	0.142857	44.9
ndhB_p5	ndhB	5	TATAGGCCCGAGTTGGACAT	TAGCCGGAAAGTTATGTGGC	20	20	50.0	50.0	57.9	58.0	0.0	5.0	0.129790	144302	146516	2884-2884	0.142857	44.7