primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	138	333	301-341	1.000000	90.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	138	333	315-355	1.000000	90.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	138	333	314-354	1.000000	90.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	138	333	316-356	1.000000	90.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	138	333	251-291	1.000000	90.4
psbA_p1	psbA	1	GATCCACTTGGCTACATCCG	AAATTCGTGTGCTTGGGAGT	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.147663	334	1395	1319-1359	1.000000	83.1
psbA_p2	psbA	2	CAATCCACTGCCTTGATCCA	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.152025	334	1395	1333-1373	1.000000	83.1
psbA_p3	psbA	3	AATCCACTGCCTTGATCCAC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.153532	334	1395	1332-1372	1.000000	83.0
psbA_p4	psbA	4	ACAATCCACTGCCTTGATCC	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.153532	334	1395	1334-1374	1.000000	83.0
psbA_p5	psbA	5	GATCCACTTGGCTACATCCG	TGCTTGGGAGTCCCTGATAA	20	20	55.0	50.0	58.1	58.0	0.0	28.7	0.163506	334	1395	1310-1350	1.000000	83.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCCCTCGCTTCATCCAAATG	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.0	56.5	0.0	13.9	1.591763	4204	4913	834-886	1.000000	71.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCGATGGTATGGACGAATCC	AGGAACTGTTCGGGATCTTT	20	20	55.0	45.0	57.9	56.5	0.0	13.9	1.629363	4204	4913	851-903	1.000000	71.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CGATGGTATGGACGAATCCC	AGGAACTGTTCGGGATCTTT	20	20	55.0	45.0	57.9	56.5	0.0	13.9	1.629363	4204	4913	850-902	1.000000	71.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	4204	4913	789-841	1.000000	71.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAAGCCGAGTACTCTACCGT	AGGAACTGTTCGGGATCTTT	20	20	50.0	45.0	58.2	56.5	20.3	13.9	1.717497	4204	4913	790-842	1.000000	70.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGCTTTCTACCACAGCGTTT	TGGAAGTATAGATGGGGCGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.135316	6018	7293	937-1441	1.000000	86.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TTGCTTTCTACCACAGCGTT	TGGAAGTATAGATGGGGCGT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.135316	6018	7293	938-1442	1.000000	86.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TGCTTTCTACCACAGCGTTT	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.094301	6018	7293	1453-1955	1.000000	86.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TTGCTTTCTACCACAGCGTT	CTTACAGCAGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.094301	6018	7293	1454-1956	1.000000	86.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GATTCCCGCGTGATACACTT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.1	57.9	4.9	0.0	0.120162	6018	7293	1810-2312	1.000000	86.1
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9851	9986	216-247	1.000000	73.2
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9851	9986	219-250	1.000000	72.9
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9851	9986	222-253	1.000000	72.3
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9851	9986	225-256	1.000000	71.9
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9851	9986	220-251	1.000000	70.6
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	13562	14676	1170-1228	1.000000	85.4
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	13562	14676	1226-1284	1.000000	84.5
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	13562	14676	1186-1244	1.000000	84.4
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	13562	14676	1171-1229	1.000000	84.2
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.2	57.9	0.0	21.3	0.256114	13562	14676	1230-1288	1.000000	83.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28312	29040	647-791	1.000000	87.7
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	28312	29040	708-852	1.000000	85.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28312	29040	665-809	1.000000	85.4
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	28312	29040	726-870	1.000000	83.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	28312	29040	646-790	1.000000	81.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.6	58.5	0.0	5.4	0.870603	30384	31071	736-808	1.000000	79.6
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGGATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	20	20	50.0	50.0	57.6	57.0	0.0	5.4	1.390757	30384	31071	737-809	1.000000	75.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	20	19	50.0	52.6	57.6	57.8	0.0	5.4	1.625006	30384	31071	735-807	1.000000	73.5
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GGAACTAGGATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	57.7	58.5	0.0	5.4	1.811914	30384	31071	742-814	1.000000	72.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	20	21	50.0	47.6	57.6	58.5	0.0	5.4	1.884745	30384	31071	738-810	1.000000	71.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	31498	32247	419-884	1.000000	85.5
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.2	58.0	0.0	0.0	0.220324	31498	32247	454-919	1.000000	85.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.273369	31498	32247	384-849	1.000000	84.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	31498	32247	416-881	1.000000	84.4
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	20	20	60.0	50.0	58.2	57.9	0.0	0.0	0.317890	31498	32247	419-884	1.000000	84.3
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	AAGGGCCCTTTTAACTCAGC	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	58.1	58.1	32.5	9.5	0.128284	32320	33763	1619-1656	1.000000	83.3
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	AGACCGGACCATCCTACAAA	20	20	50.0	50.0	57.9	58.1	0.0	9.5	0.156553	32320	33763	1615-1652	1.000000	83.1
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AAGGGCCCTTTTAACTCAGC	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	58.1	57.8	32.5	9.5	0.231612	32320	33763	1626-1663	1.000000	82.5
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCATC	20	20	50.0	55.0	57.9	57.8	0.0	9.5	0.259880	32320	33763	1622-1659	1.000000	82.3
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	AAGGGCCCTTTTAACTCAGC	AGAGCAATAGACCGGACCAT	20	20	50.0	50.0	58.1	58.2	32.5	9.5	0.280554	32320	33763	1627-1664	1.000000	82.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.230316	51587	52671	1204-1316	1.000000	84.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.4	58.1	0.0	0.0	0.715163	51587	52671	1191-1303	1.000000	80.6
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.0	0.0	0.806132	51587	52671	1189-1301	1.000000	79.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.3	58.1	0.0	0.0	0.828958	51587	52671	1199-1311	1.000000	79.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.8	58.1	5.2	0.0	0.936224	51587	52671	1186-1298	1.000000	78.9
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	63628	64586	839-1188	1.000000	78.9
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	63628	64586	826-1175	1.000000	78.6
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	63628	64586	840-1189	1.000000	78.2
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	63628	64586	827-1176	1.000000	77.9
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	63628	64586	844-1193	1.000000	77.3
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.060086	65368	66372	1092-1177	1.000000	85.2
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097701	65368	66372	1105-1190	1.000000	84.9
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	6.4	0.0	0.134501	65368	66372	1095-1180	1.000000	84.6
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.165095	65368	66372	1015-1100	1.000000	84.5
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.172116	65368	66372	1108-1193	1.000000	84.3
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	93217	94469	500-1512	1.000000	89.5
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.237960	93217	94469	499-1511	1.000000	89.5
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	93217	94469	502-1514	1.000000	89.5
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	20	20	45.0	50.0	57.8	58.0	0.7	15.9	0.239330	93217	94469	501-1513	1.000000	89.5
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.2	58.0	0.7	15.9	0.240071	93217	94469	504-1515	0.923077	86.0
ycf1_p1	ycf1	1	CCCGGAACTTCTTCGTAGTG	GGGAATAAGAGGATTGGCCG	20	20	55.0	55.0	58.0	58.1	0.7	0.0	0.110270	109719	112007	2724-2724	0.076923	42.2
ycf1_p2	ycf1	2	CCGGAACTTCTTCGTAGTGG	GGGAATAAGAGGATTGGCCG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.110270	109719	112007	2723-2723	0.076923	42.2
ycf1_p3	ycf1	3	ATCTCCCCAGGTAGGATTCG	GGGAATAAGAGGATTGGCCG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.119345	109719	112007	2880-2880	0.076923	42.1
ycf1_p4	ycf1	4	TCAAAGCGAGCCCTTTTCTT	GGGAATAAGAGGATTGGCCG	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.121184	109719	112007	2933-2933	0.076923	42.1
ycf1_p5	ycf1	5	AACCCATGACCAATACGAGC	GGGAATAAGAGGATTGGCCG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.140827	109719	112007	2764-2764	0.076923	42.0
ccsA-ndhD_p1	ccsA-ndhD	1	TGGATATGCTATTTTGGGGTCA	CAGTTGACAAGGTCGAGGTT	22	20	40.9	50.0	57.2	57.8	0.0	0.0	3.045633	117516	117739	347-368	1.000000	65.1
ccsA-ndhD_p2	ccsA-ndhD	2	TGGATATGCTATTTTGGGGTCA	GTTGACAAGGTCGAGGTTCT	22	20	40.9	50.0	57.2	57.5	0.0	0.0	3.333181	117516	117739	345-366	1.000000	62.9
ccsA-ndhD_p3	ccsA-ndhD	3	TGGATATGCTATTTTGGGGTCA	AGTTGACAAGGTCGAGGTTC	22	20	40.9	50.0	57.2	57.5	0.0	0.0	3.333181	117516	117739	346-367	1.000000	62.8
ccsA-ndhD_p4	ccsA-ndhD	4	TGGATATGCTATTTTGGGGTCAA	CAGTTGACAAGGTCGAGGTT	23	20	39.1	50.0	57.9	57.8	0.0	0.0	3.376561	117516	117739	347-368	1.000000	62.5
ccsA-ndhD_p5	ccsA-ndhD	5	TTGGATATGCTATTTTGGGGTCA	CAGTTGACAAGGTCGAGGTT	23	20	39.1	50.0	57.9	57.8	0.0	0.0	3.376561	117516	117739	348-369	1.000000	62.4