primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhB_p1	ndhB	1	TCGCGTCACAAGAAGAGAAG	TCAATCTCAAATATTACTCCGA	20	22	50.0	31.8	57.9	52.5	5.8	0.0	7.589124	625	2995	2426-2426	0.500000	20.0
ndhB_p2	ndhB	2	TGCGGAGACAAACTAGTTGG	TCAATCTCAAATATTACTCCGA	20	22	50.0	31.8	57.8	52.5	9.3	0.0	7.701063	625	2995	2492-2492	0.500000	20.0
ndhB_p3	ndhB	3	TCGCGTCACAAGAAGAGAAG	TTCAATCTCAAATATTACTCCGA	20	23	50.0	30.4	57.9	53.3	5.8	0.0	7.723623	625	2995	2427-2427	0.500000	20.0
ndhB_p4	ndhB	4	AGTTCGCGTCACAAGAAGAG	TCAATCTCAAATATTACTCCGA	20	22	50.0	31.8	58.2	52.5	5.8	0.0	7.765063	625	2995	2429-2429	0.500000	20.0
ndhB_p5	ndhB	5	CGCGTCACAAGAAGAGAAGT	TCAATCTCAAATATTACTCCGA	20	22	50.0	31.8	58.2	52.5	0.0	0.0	7.765063	625	2995	2425-2425	0.500000	20.0
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	ACGTTCGGTTCTGGATTAGC	AACACGGAATTACGCCCAAT	20	20	50.0	45.0	58.0	58.2	0.0	10.7	0.176991	11526	11692	351-351	1.000000	80.4
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	ACGTTCGGTTCTGGATTAGC	ACGGAATTACGCCCAATAGG	20	20	50.0	50.0	58.0	57.8	0.0	10.7	0.255814	11526	11692	348-348	1.000000	79.8
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	TACTGATGCGGGTTCGATTC	AACACGGAATTACGCCCAAT	20	20	50.0	45.0	57.8	58.2	0.0	10.7	0.389974	11526	11692	281-281	1.000000	79.2
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CGCCGTCGACTATAACCTTT	AACACGGAATTACGCCCAAT	20	20	50.0	45.0	57.8	58.2	14.7	10.7	0.370312	11526	11692	313-313	1.000000	79.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CTACTGATGCGGGTTCGATT	AACACGGAATTACGCCCAAT	20	20	50.0	45.0	57.8	58.2	0.0	10.7	0.396089	11526	11692	282-282	1.000000	79.1
atpA-atpF_p1	atpA-atpF	1	TTTGCTTGCGAATGATGCTG	GAGCCATGGATAGCAAGTCC	20	20	45.0	55.0	58.0	58.1	0.0	18.4	0.125121	13392	13477	164-164	1.000000	81.4
atpA-atpF_p2	atpA-atpF	2	TTTGCTTGCGAATGATGCTG	CCATGGATAGCAAGTCCGAC	20	20	45.0	55.0	58.0	58.1	0.0	5.6	0.143586	13392	13477	161-161	1.000000	81.3
atpA-atpF_p3	atpA-atpF	3	TTTGCTTGCGAATGATGCTG	TCAACTGCACTTGCGAATGA	20	20	45.0	45.0	58.0	58.4	0.0	1.6	0.422602	13392	13477	208-208	1.000000	78.5
atpA-atpF_p4	atpA-atpF	4	TGCTGCTAATTTCGTCAGGT	GAGCCATGGATAGCAAGTCC	20	20	45.0	55.0	57.5	58.1	0.0	18.4	0.583151	13392	13477	149-149	1.000000	78.0
atpA-atpF_p5	atpA-atpF	5	TGCTGCTAATTTCGTCAGGT	CCATGGATAGCAAGTCCGAC	20	20	45.0	55.0	57.5	58.1	0.0	5.6	0.601615	13392	13477	146-146	1.000000	77.9
atpH-atpI_p1	atpH-atpI	1	GCCTTCTACTTCTGGCTGTC	TATTTCAGCCGACGAGTTGG	20	20	55.0	50.0	58.0	58.0	0.0	5.1	0.021721	15362	16178	1108-1124	1.000000	84.5
atpH-atpI_p2	atpH-atpI	2	GCCTTCTACTTCTGGCTGTC	AGGTGCCAGAAATCTGCAAA	20	20	55.0	45.0	58.0	58.0	0.0	4.9	0.017490	15362	16178	1522-1538	1.000000	83.7
atpH-atpI_p3	atpH-atpI	3	GAGGGAGTAGCGATCTCACT	AGGTGCCAGAAATCTGCAAA	20	20	55.0	45.0	58.0	58.0	9.6	4.9	0.024692	15362	16178	2026-2031	1.000000	83.2
atpH-atpI_p4	atpH-atpI	4	GAGGGGTAAAGGTGCAACAA	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	3.6	4.9	0.010646	15362	16178	1685-1685	0.500000	59.9
atpH-atpI_p5	atpH-atpI	5	GAGGGGTAAAGGTGCAACAA	TATTTCAGCCGACGAGTTGG	20	20	50.0	50.0	58.0	58.0	3.6	5.1	0.014876	15362	16178	1271-1271	0.500000	59.9
rpoB-trnD-GUC_p1	rpoB-trnD-GUC	1	CAAGTTCTGGTAATACGGGCA	GAGTTCATCGGGATTGACGG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.447513	28492	29127	735-740	1.000000	71.8
rpoB-trnD-GUC_p2	rpoB-trnD-GUC	2	CAAGTTCTGGTAATACGGGCA	GGAGTTCATCGGGATTGACG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.447513	28492	29127	736-741	1.000000	71.8
rpoB-trnD-GUC_p3	rpoB-trnD-GUC	3	TGCAAGTTCTGGTAATACGGG	GAGTTCATCGGGATTGACGG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.447513	28492	29127	737-742	1.000000	71.8
rpoB-trnD-GUC_p4	rpoB-trnD-GUC	4	TGCAAGTTCTGGTAATACGGG	GGAGTTCATCGGGATTGACG	21	20	47.6	55.0	58.0	58.4	0.0	0.0	1.447513	28492	29127	738-743	1.000000	71.8
rpoB-trnD-GUC_p5	rpoB-trnD-GUC	5	CAAGTTCTGGTAATACGGGCA	CAACTTCCGTCTTGACAGGG	21	20	47.6	55.0	58.0	58.5	0.0	1.6	1.506506	28492	29127	703-708	1.000000	71.5
psbM-petN_p1	psbM-petN	1	CCACTTACATGCCCCAAACT	ATTGGATGTTGTTTCCGCCT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.153289	29982	31125	2216-2216	0.500000	58.8
psbM-petN_p2	psbM-petN	2	CCACTTACATGCCCCAAACT	AAGACGGAGGACTGCAAATC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.177249	29982	31125	2145-2145	0.500000	58.6
psbM-petN_p3	psbM-petN	3	CCACTTACATGCCCCAAACT	TGTTGTTTCCGCCTGGATAG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.184594	29982	31125	2210-2210	0.500000	58.5
psbM-petN_p4	psbM-petN	4	CCACTTACATGCCCCAAACT	TTGTAGTTTGGGGACGAAGC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.189758	29982	31125	1579-1579	0.500000	58.5
psbM-petN_p5	psbM-petN	5	CCACTTACATGCCCCAAACT	CATTTCATGTGCCTCGATGC	20	20	50.0	50.0	58.1	57.9	0.0	5.7	0.191992	29982	31125	1942-1942	0.500000	58.5
rps4-trnL-UAA_p1	rps4-trnL-UAA	1	TCAGTTTCAAACGAGGTCCTC	CTCCACATCCAGGCTCAATC	21	20	47.6	55.0	57.9	58.0	9.1	0.0	1.143089	46796	47425	723-723	1.000000	73.6
rps4-trnL-UAA_p2	rps4-trnL-UAA	2	TCAGTTTCAAACGAGGTCCTC	GCCCGATAAATGATCCCAGG	21	20	47.6	55.0	57.9	58.2	9.1	0.0	1.281257	46796	47425	777-777	1.000000	72.3
rps4-trnL-UAA_p3	rps4-trnL-UAA	3	TCAGTTTCAAACGAGGTCCTC	GGGCTACCACTTGATACGTC	21	20	47.6	55.0	57.9	57.8	9.1	0.0	1.328121	46796	47425	742-742	1.000000	72.1
rps4-trnL-UAA_p4	rps4-trnL-UAA	4	TCAGTTTCAAACGAGGTCCT	CTCCACATCCAGGCTCAATC	20	20	45.0	55.0	56.7	58.0	0.0	0.0	1.328635	46796	47425	723-723	1.000000	72.1
rps4-trnL-UAA_p5	rps4-trnL-UAA	5	TCAGTTTCAAACGAGGTCCTC	CCACATCCAGGCTCAATCTG	21	20	47.6	55.0	57.9	58.3	9.1	0.0	1.430896	46796	47425	721-721	1.000000	71.3
trnV-UAC_p1	trnV-UAC	1	CATACGGTGTGCTAGCAACT	GCTGAGTCAAGTAGGCTGTC	20	20	50.0	55.0	58.0	58.0	21.3	0.0	0.013341	50829	51549	1028-1038	1.000000	86.6
trnV-UAC_p2	trnV-UAC	2	CATACGGTGTGCTAGCAACT	GCTTCGGCAGTCTGGAAATA	20	20	50.0	50.0	58.0	58.0	21.3	1.1	0.034321	50829	51549	1231-1246	1.000000	85.5
trnV-UAC_p3	trnV-UAC	3	GGCAACAAATCCGGAAATCG	GCTGAGTCAAGTAGGCTGTC	20	20	50.0	55.0	58.1	58.0	9.0	0.0	0.095131	50829	51549	1586-1595	1.000000	84.5
trnV-UAC_p4	trnV-UAC	4	CTCGTCATACTGGAGAGGGT	GCTGAGTCAAGTAGGCTGTC	20	20	55.0	55.0	57.9	58.0	0.0	0.0	0.063247	50829	51549	1392-1392	0.500000	59.5
trnV-UAC_p5	trnV-UAC	5	CTCGTCATACTGGAGAGGGT	GCTTCGGCAGTCTGGAAATA	20	20	55.0	50.0	57.9	58.0	0.0	1.1	0.084226	50829	51549	1595-1595	0.500000	59.3
psaI-ycf4_p1	psaI-ycf4	1	CGTACCCCTAGTTGGTTTGG	CACCGCAGACAAGGATACAA	20	20	55.0	50.0	57.9	57.9	6.5	0.0	0.196843	57960	58391	600-608	1.000000	81.9
psaI-ycf4_p2	psaI-ycf4	2	CGTACCCCTAGTTGGTTTGG	AATTCGCAACTGTACGGGAA	20	20	55.0	45.0	57.9	57.8	6.5	6.7	0.276388	57960	58391	570-578	1.000000	81.4
psaI-ycf4_p3	psaI-ycf4	3	CGTACCCCTAGTTGGTTTGG	GAATTCGCAACTGTACGGGA	20	20	55.0	50.0	57.9	58.3	6.5	6.7	0.386343	57960	58391	571-579	1.000000	80.5
psaI-ycf4_p4	psaI-ycf4	4	CGTACCCCTAGTTGGTTTGG	CAAGCCCAACATGAATTCGC	20	20	55.0	50.0	57.9	58.4	6.5	0.0	0.471115	57960	58391	583-591	1.000000	79.8
psaI-ycf4_p5	psaI-ycf4	5	CCCCTAGTTGGTTTGGTGTT	CACCGCAGACAAGGATACAA	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.468320	57960	58391	596-604	1.000000	79.7
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GCGTTCTACCGAACTGAACT	GGTAGCGTGTTTGTTTTGGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.190107	64584	64782	283-283	1.000000	81.6
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	CCTGGGGTAGACGGTAGTAG	GGTAGCGTGTTTGTTTTGGG	20	20	60.0	50.0	58.0	57.9	0.0	0.0	0.149913	64584	64782	350-350	1.000000	81.3
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	TAGTAGACACGCCCTGTAGG	GGTAGCGTGTTTGTTTTGGG	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.176812	64584	64782	336-336	1.000000	81.2
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	AATCCTGGGGTAGACGGTAG	GGTAGCGTGTTTGTTTTGGG	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.211996	64584	64782	353-353	1.000000	80.8
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTAGGATTCGAACCTACGGC	GGTAGCGTGTTTGTTTTGGG	20	20	55.0	50.0	57.9	57.9	26.6	0.0	0.259648	64584	64782	321-321	1.000000	80.7
rpl2-rpl23_p1	rpl2-rpl23	1	ATTGCTGCGACCACTTCTAG	TGCGAGGCAGGTATATCTGA	20	20	50.0	50.0	58.0	58.0	0.0	17.5	0.020360	83745	83813	591-591	1.000000	83.1
rpl2-rpl23_p2	rpl2-rpl23	2	CTGGCTACTCTTCCGTCAAC	TGCGAGGCAGGTATATCTGA	20	20	55.0	50.0	58.0	58.0	0.0	17.5	0.006955	83745	83813	695-695	1.000000	82.8
rpl2-rpl23_p3	rpl2-rpl23	3	CTGGCTACTCTTCCGTCAAC	AGTCTATTCGCCTGTTGCAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.018067	83745	83813	541-541	1.000000	82.7
rpl2-rpl23_p4	rpl2-rpl23	4	CTGGCTACTCTTCCGTCAAC	GAGGAGAGGATCGATGGGAA	20	20	55.0	55.0	58.0	58.0	0.0	0.5	0.004836	83745	83813	628-628	0.500000	60.0
rpl2-rpl23_p5	rpl2-rpl23	5	ATTGCTGCGACCACTTCTAG	GAGGAGAGGATCGATGGGAA	20	20	50.0	55.0	58.0	58.0	0.0	0.5	0.018241	83745	83813	524-524	0.500000	59.9
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	TGACGGAAGAGAGAGACTCC	GTGATGGAGGTCACAGGTTC	20	20	55.0	55.0	57.9	57.9	0.0	2.4	0.217432	109345	110026	670-751	1.000000	87.7
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	TGACGGAAGAGAGAGACTCC	TGATGGAGGTCACAGGTTCA	20	20	55.0	50.0	57.9	58.3	0.0	0.0	0.388504	109345	110026	669-750	1.000000	86.3
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	ACGGAAGAGAGAGACTCCTC	GTGATGGAGGTCACAGGTTC	20	20	55.0	55.0	57.6	57.9	11.1	2.4	0.513914	109345	110026	668-749	1.000000	85.3
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	GACGGAAGAGAGAGACTCCT	GTGATGGAGGTCACAGGTTC	20	20	55.0	55.0	57.6	57.9	0.0	2.4	0.513914	109345	110026	669-750	1.000000	85.3
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	ACGGAAGAGAGAGACTCCTC	TGATGGAGGTCACAGGTTCA	20	20	55.0	50.0	57.6	58.3	11.1	0.0	0.684986	109345	110026	667-748	1.000000	83.9
trnP-GGG-trnL-UAG_p1	trnP-GGG-trnL-UAG	1	GTGACTTCGCTATCAGTCGG	CTCTGGCACTGCTTCCTAAG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.192524	110101	110337	380-381	1.000000	81.0
trnP-GGG-trnL-UAG_p2	trnP-GGG-trnL-UAG	2	GTGACTTCGCTATCAGTCGG	CTGGCACTGCTTCCTAAGAG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.192524	110101	110337	378-379	1.000000	81.0
trnP-GGG-trnL-UAG_p3	trnP-GGG-trnL-UAG	3	GTGACTTCGCTATCAGTCGG	AGAGCAGCGTGTCTACCTAT	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.231219	110101	110337	362-363	1.000000	80.8
trnP-GGG-trnL-UAG_p4	trnP-GGG-trnL-UAG	4	TGACTTCGCTATCAGTCGGA	CTCTGGCACTGCTTCCTAAG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.274885	110101	110337	379-380	1.000000	80.3
trnP-GGG-trnL-UAG_p5	trnP-GGG-trnL-UAG	5	TGACTTCGCTATCAGTCGGA	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.274885	110101	110337	377-378	1.000000	80.3
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	CTCTTAGGAAGCAGTGCCAG	GGATAGAAGCGCGTTCCATA	20	20	55.0	50.0	58.0	57.6	12.6	8.4	0.455224	110417	110767	415-433	1.000000	83.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	CTCTTAGGAAGCAGTGCCAG	TATGGATAGAAGCGCGTTCC	20	20	55.0	50.0	58.0	57.6	12.6	8.4	0.455224	110417	110767	418-436	1.000000	83.9
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	CTTAGGAAGCAGTGCCAGAG	GGATAGAAGCGCGTTCCATA	20	20	55.0	50.0	58.0	57.6	12.6	8.4	0.455224	110417	110767	413-431	1.000000	83.9
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	CTTAGGAAGCAGTGCCAGAG	TATGGATAGAAGCGCGTTCC	20	20	55.0	50.0	58.0	57.6	12.6	8.4	0.455224	110417	110767	416-434	1.000000	83.9
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	ATAGGTAGACACGCTGCTCT	GGATAGAAGCGCGTTCCATA	20	20	50.0	50.0	57.9	57.6	0.0	8.4	0.493919	110417	110767	431-449	1.000000	83.3
rps15-ycf1_p1	rps15-ycf1	1	CTACCGCGACTCACGTTAAA	GCCACCATATCGATCTCGTC	20	20	50.0	55.0	58.0	58.0	8.2	0.0	0.048266	120515	120634	1134-1155	1.000000	85.8
rps15-ycf1_p2	rps15-ycf1	2	GGGTTGTAGTAGGCGTTAGC	GCCACCATATCGATCTCGTC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.084122	120515	120634	971-992	1.000000	85.7
rps15-ycf1_p3	rps15-ycf1	3	CTACCGCGACTCACGTTAAA	GGTTTGATAGGCCTCCCAAG	20	20	50.0	55.0	58.0	57.9	8.2	9.7	0.090831	120515	120634	1207-1228	1.000000	85.1
rps15-ycf1_p4	rps15-ycf1	4	CTACCGCGACTCACGTTAAA	AAACTCTCTTGCAACCCTGG	20	20	50.0	50.0	58.0	58.0	8.2	0.0	0.045356	120515	120634	1086-1086	0.500000	59.6
rps15-ycf1_p5	rps15-ycf1	5	GGGTTGTAGTAGGCGTTAGC	AAACTCTCTTGCAACCCTGG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.081212	120515	120634	923-923	0.500000	59.4
chlN-chlL_p1	chlN-chlL	1	TCGCATTCGAAAGTGAGAAT	AGATGCTGCGCTTGATTTTG	20	20	40.0	45.0	55.5	57.7	12.2	9.7	2.777081	131183	131262	146-146	1.000000	62.2
chlN-chlL_p2	chlN-chlL	2	TCGCATTCGAAAGTGAGAAT	CAAGATGCTGCGCTTGATTT	20	20	40.0	45.0	55.5	57.7	12.2	22.7	2.777081	131183	131262	148-148	1.000000	62.1
chlN-chlL_p3	chlN-chlL	3	TCGCATTCGAAAGTGAGAAT	GCTGCGCTTGATTTTGCAAT	20	20	40.0	45.0	55.5	58.9	12.2	9.7	3.430534	131183	131262	142-142	1.000000	57.1
chlN-chlL_p4	chlN-chlL	4	TCGCATTCGAAAGTGAGAAT	AAGATGCTGCGCTTGATTTT	20	20	40.0	40.0	55.5	57.0	12.2	9.7	3.527686	131183	131262	147-147	1.000000	56.1
chlN-chlL_p5	chlN-chlL	5	TCGCATTCGAAAGTGAGAAT	ATGCTGCGCTTGATTTTGC	20	19	40.0	47.4	55.5	58.3	12.2	9.7	3.757983	131183	131262	144-144	1.000000	54.4