primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
EI144_pgp62_p1	EI144_pgp62	1	TCGGTCCATATCCGGTGTAA	ACGCCAAAACTAGGAGCAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.094645	5014	6308	2544-2630	0.750000	79.5
EI144_pgp62_p2	EI144_pgp62	2	TTCGGTCCATATCCGGTGTA	ACGCCAAAACTAGGAGCAAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.094645	5014	6308	2545-2631	0.750000	79.5
EI144_pgp62_p3	EI144_pgp62	3	GAGAGGACCGAAAAGGACAC	ACGCCAAAACTAGGAGCAAA	20	20	55.0	45.0	57.9	58.0	0.0	0.0	0.106066	5014	6308	2490-2576	0.750000	79.5
EI144_pgp62_p4	EI144_pgp62	4	TCGGTCCATATCCGGTGTAA	CAAAAAGAGCAGCGCCTAAC	20	20	50.0	50.0	57.9	58.0	0.0	2.3	0.101202	5014	6308	2688-2688	0.250000	49.2
EI144_pgp62_p5	EI144_pgp62	5	TTCGGTCCATATCCGGTGTA	CAAAAAGAGCAGCGCCTAAC	20	20	50.0	50.0	57.9	58.0	0.0	2.3	0.101202	5014	6308	2689-2689	0.250000	49.2
EI144_pgp61_p1	EI144_pgp61	1	GTCTTCCGTTGCTCCTAAGG	ATCCAGTTAGGCAAGTCCTT	20	20	55.0	45.0	58.0	56.1	0.0	0.0	1.892225	6423	7860	2346-2346	0.250000	34.9
EI144_pgp61_p2	EI144_pgp61	2	TGCTCCTAAGGGAAGGTCAA	ATCCAGTTAGGCAAGTCCTT	20	20	50.0	45.0	58.0	56.1	0.0	0.0	1.919548	6423	7860	2337-2337	0.250000	34.6
EI144_pgp61_p3	EI144_pgp61	3	TTGCTCCTAAGGGAAGGTCA	ATCCAGTTAGGCAAGTCCTT	20	20	50.0	45.0	58.0	56.1	0.0	0.0	1.919548	6423	7860	2338-2338	0.250000	34.6
EI144_pgp61_p4	EI144_pgp61	4	TCTGTCTTCCGTTGCTCCTA	ATCCAGTTAGGCAAGTCCTT	20	20	50.0	45.0	58.1	56.1	0.0	0.0	1.961444	6423	7860	2349-2349	0.250000	34.3
EI144_pgp61_p5	EI144_pgp61	5	GTCTTCCGTTGCTCCTAAGG	AATCCAGTTAGGCAAGTCCTT	20	21	55.0	42.9	58.0	56.9	0.0	0.0	2.110217	6423	7860	2347-2347	0.250000	33.1
EI144_pgp60_p1	EI144_pgp60	1	GGACTTGCCTAACTGGATTT	TGACGCTGGAAACAAAACTG	20	20	45.0	45.0	55.3	57.2	0.0	0.0	3.521406	8620	8739	1147-1147	0.250000	21.8
EI144_pgp60_p2	EI144_pgp60	2	GGACTTGCCTAACTGGATTTT	TGACGCTGGAAACAAAACTG	21	20	42.9	45.0	56.1	57.2	0.0	0.0	3.714689	8620	8739	1147-1147	0.250000	20.3
EI144_pgp60_p3	EI144_pgp60	3	GGACTTGCCTAACTGGATTT	GACGCTGGAAACAAAACTGA	20	20	45.0	45.0	55.3	56.9	0.0	0.0	3.805948	8620	8739	1146-1146	0.250000	19.6
EI144_pgp60_p4	EI144_pgp60	4	GGACTTGCCTAACTGGATTTTTC	TGACGCTGGAAACAAAACTG	23	20	43.5	45.0	57.9	57.2	0.0	0.0	3.902870	8620	8739	1147-1147	0.250000	18.8
EI144_pgp60_p5	EI144_pgp60	5	TGTCCCTTATTCTTTTATGCGGA	TGACGCTGGAAACAAAACTG	23	20	39.1	45.0	57.9	57.2	0.0	0.0	3.947775	8620	8739	725-725	0.250000	18.4
EI144_pgp58_p1	EI144_pgp58	1	GGTGGTCGTGATCAAGAGTC	CCAACCATCACCACCAAAGA	20	20	55.0	50.0	58.0	58.0	11.9	0.0	0.008375	10734	12095	2189-2791	0.500000	69.3
EI144_pgp58_p2	EI144_pgp58	2	GGTGGTCGTGATCAAGAGTC	TCCAACCATCACCACCAAAG	20	20	55.0	50.0	58.0	58.0	11.9	0.0	0.008375	10734	12095	2190-2792	0.500000	69.3
EI144_pgp58_p3	EI144_pgp58	3	CAACTGGTTTTGCTTGGTGG	CCAACCATCACCACCAAAGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.067419	10734	12095	2170-2772	0.500000	68.9
EI144_pgp58_p4	EI144_pgp58	4	CAACTGGTTTTGCTTGGTGG	TCCAACCATCACCACCAAAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.067419	10734	12095	2171-2773	0.500000	68.9
EI144_pgp58_p5	EI144_pgp58	5	GGTGGTCGTGATCAAGAGTC	CGATGGATTCAAAGTCGGGT	20	20	55.0	50.0	58.0	58.0	11.9	0.0	0.026507	10734	12095	2141-2141	0.250000	49.8
EI144_pgp51_p1	EI144_pgp51	1	CGGAGTAGAGCAGTCTGGTA	ACTGTAGGAGCAGGAGTTGT	20	20	55.0	50.0	58.0	58.0	4.1	0.0	0.056723	16681	17710	1639-1671	0.500000	71.0
EI144_pgp51_p2	EI144_pgp51	2	GGTTAATGTCGTGGGTTCGA	ACTGTAGGAGCAGGAGTTGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.100071	16681	17710	1688-1715	0.500000	70.9
EI144_pgp51_p3	EI144_pgp51	3	AAAGCGGATGTAGCTCAGTG	ACTGTAGGAGCAGGAGTTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032641	16681	17710	1728-1728	0.250000	49.7
EI144_pgp51_p4	EI144_pgp51	4	GAATCCCATCATCCGCTTGT	ACTGTAGGAGCAGGAGTTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044033	16681	17710	1670-1670	0.250000	49.6
EI144_pgp51_p5	EI144_pgp51	5	AGGCTTAAAGCGGATGTAGC	ACTGTAGGAGCAGGAGTTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.061978	16681	17710	1734-1734	0.250000	49.5
EI144_pgp35_p1	EI144_pgp35	1	CCAGTTCGAATCCGGGTATC	AAAGATTGTCCGCTTCCAGG	20	20	55.0	50.0	57.8	58.2	0.0	0.0	0.324012	37062	38025	1656-1656	0.250000	47.4
EI144_pgp35_p2	EI144_pgp35	2	CCAGTTCGAATCCGGGTATC	TCATTTGTTGGCGCTCCTAA	20	20	55.0	45.0	57.8	57.8	0.0	7.6	0.343061	37062	38025	1845-1845	0.250000	47.3
EI144_pgp35_p3	EI144_pgp35	3	CCAGTTCGAATCCGGGTATC	TTGTTGGCGCTCCTAATTCA	20	20	55.0	45.0	57.8	57.8	0.0	7.6	0.343061	37062	38025	1841-1841	0.250000	47.3
EI144_pgp35_p4	EI144_pgp35	4	CAGTTCGAATCCGGGTATCG	AAAGATTGTCCGCTTCCAGG	20	20	55.0	50.0	58.2	58.2	15.4	0.0	0.406326	37062	38025	1655-1655	0.250000	46.7
EI144_pgp35_p5	EI144_pgp35	5	GTTCGAATCCGGGTATCGTC	AAAGATTGTCCGCTTCCAGG	20	20	55.0	50.0	58.2	58.2	19.0	0.0	0.409981	37062	38025	1653-1653	0.250000	46.7
EI144_pgp34_p1	EI144_pgp34	1	CCGGCTAATGATGATTCCGT	GGGCCTATTGCTCCTAAACC	20	20	50.0	55.0	57.8	58.0	4.7	0.8	0.187172	38171	39662	1944-1944	0.250000	48.5
EI144_pgp34_p2	EI144_pgp34	2	CCGGCTAATGATGATTCCGT	TTGCCTTTCCTGAAGCTGTT	20	20	50.0	45.0	57.8	57.9	4.7	0.0	0.241610	38171	39662	1979-1979	0.250000	48.1
EI144_pgp34_p3	EI144_pgp34	3	CCGGCTAATGATGATTCCGT	GCTTCTGGTTGTCGAGCTAA	20	20	50.0	50.0	57.8	57.9	4.7	0.0	0.250118	38171	39662	2007-2007	0.250000	48.0
EI144_pgp34_p4	EI144_pgp34	4	CCGGCTAATGATGATTCCGT	CTTCTGCTTCTGGTTGTCGA	20	20	50.0	50.0	57.8	57.8	4.7	0.0	0.324686	38171	39662	2012-2012	0.250000	47.4
EI144_pgp34_p5	EI144_pgp34	5	CCGGCTAATGATGATTCCGT	TTCTGCTTCTGGTTGTCGAG	20	20	50.0	50.0	57.8	57.8	4.7	0.0	0.324686	38171	39662	2011-2011	0.250000	47.4
trnS(UGA)-EI144_pgp28_p1	trnS(UGA)-EI144_pgp28	1	GGATGTAGCGCAGTTTGGTA	TGTTTGGGAAGGTCAGTGTT	20	20	50.0	45.0	58.0	57.2	11.5	0.0	0.837293	43184	43595	1054-1054	0.250000	43.3
trnS(UGA)-EI144_pgp28_p2	trnS(UGA)-EI144_pgp28	2	CAAGGGTCACAGGTTCGAAT	TGTTTGGGAAGGTCAGTGTT	20	20	50.0	45.0	57.8	57.2	0.0	0.0	1.003755	43184	43595	1015-1015	0.250000	42.0
trnS(UGA)-EI144_pgp28_p3	trnS(UGA)-EI144_pgp28	3	CCGAAACTTGGAGAGGTGTC	TGTTTGGGAAGGTCAGTGTT	20	20	55.0	45.0	58.2	57.2	0.0	0.0	1.021726	43184	43595	986-986	0.250000	41.8
trnS(UGA)-EI144_pgp28_p4	trnS(UGA)-EI144_pgp28	4	GGATGTAGCGCAGTTTGGTA	ATGTTTGGGAAGGTCAGTGT	20	20	50.0	45.0	58.0	56.9	11.5	0.0	1.069406	43184	43595	1055-1055	0.250000	41.4
trnS(UGA)-EI144_pgp28_p5	trnS(UGA)-EI144_pgp28	5	GGATGTAGCGCAGTTTGGTA	TGTTTGGGAAGGTCAGTGTTT	20	21	50.0	42.9	58.0	57.9	11.5	0.0	1.113026	43184	43595	1054-1054	0.250000	41.1
EI144_pgp28_p1	EI144_pgp28	1	GGATGTAGCGCAGTTTGGTA	CCCGTTGTTTTTGCTTCTCC	20	20	50.0	50.0	58.0	57.9	11.5	0.0	0.152743	43596	44156	1438-1454	0.500000	69.0
EI144_pgp28_p2	EI144_pgp28	2	CAAGGGTCACAGGTTCGAAT	CCCGTTGTTTTTGCTTCTCC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.319205	43596	44156	1399-1415	0.500000	67.8
EI144_pgp28_p3	EI144_pgp28	3	GGATGTAGCGCAGTTTGGTA	TTGCATCGCCTGTTGATTCT	20	20	50.0	45.0	58.0	58.2	11.5	0.0	0.193452	43596	44156	1872-1872	0.250000	48.5
EI144_pgp28_p4	EI144_pgp28	4	GGATGTAGCGCAGTTTGGTA	CTTGCATCGCCTGTTGATTC	20	20	50.0	50.0	58.0	57.8	11.5	0.0	0.202926	43596	44156	1873-1873	0.250000	48.4
EI144_pgp28_p5	EI144_pgp28	5	CCGAAACTTGGAGAGGTGTC	CCCGTTGTTTTTGCTTCTCC	20	20	55.0	50.0	58.2	57.9	0.0	0.0	0.337177	43596	44156	1370-1370	0.250000	47.3
EI144_pgp24_p1	EI144_pgp24	1	TATACCACCTCCACTTGCCT	AGCTTGTGAGGTTTGGGAAG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.277013	45650	46475	1473-1473	0.250000	47.8
EI144_pgp24_p2	EI144_pgp24	2	TATACCACCTCCACTTGCCT	GAAGCTTGTGAGGTTTGGGA	20	20	50.0	50.0	57.7	58.0	0.0	2.1	0.282639	45650	46475	1475-1475	0.250000	47.7
EI144_pgp24_p3	EI144_pgp24	3	TATACCACCTCCACTTGCCT	GCTTGTGAGGTTTGGGAAGA	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.282639	45650	46475	1472-1472	0.250000	47.7
EI144_pgp24_p4	EI144_pgp24	4	TATACCACCTCCACTTGCCT	CTGCAGCTTGTGAAGTTTGG	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.393718	45650	46475	1810-1810	0.250000	46.9
EI144_pgp24_p5	EI144_pgp24	5	TATACCACCTCCACTTGCCT	CGCGAAGGTGGAGATGTTAT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.488963	45650	46475	1862-1862	0.250000	46.1
EI144_pgp19_p1	EI144_pgp19	1	ACCACGTTGTAATCCATCGG	TGGTCCGAATCCCGTTAGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.160898	50914	52023	1828-1828	0.250000	48.7
EI144_pgp19_p2	EI144_pgp19	2	CCACGTTGTAATCCATCGGT	TGGTCCGAATCCCGTTAGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.160898	50914	52023	1827-1827	0.250000	48.7
EI144_pgp19_p3	EI144_pgp19	3	CCAATTTTTCCACCACGACG	TGGTCCGAATCCCGTTAGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.187244	50914	52023	2070-2070	0.250000	48.5
EI144_pgp19_p4	EI144_pgp19	4	ACCACGACGATAAGGAGCTA	TGGTCCGAATCCCGTTAGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.190800	50914	52023	2059-2059	0.250000	48.5
EI144_pgp19_p5	EI144_pgp19	5	CATTTCCTTCACGTGTTCGC	TGGTCCGAATCCCGTTAGTA	20	20	50.0	50.0	58.0	57.9	16.5	0.0	0.191885	50914	52023	2195-2195	0.250000	48.5
EI144_pgp15_p1	EI144_pgp15	1	ACAATACGAGGTGGTTCACG	TGTGACTCCTTTATTCGCGG	20	20	50.0	50.0	57.9	58.0	0.0	6.1	0.079130	53604	55216	2395-2487	0.750000	81.4
EI144_pgp15_p2	EI144_pgp15	2	ACGAGGTGGTTCACGAAAAA	TGTGACTCCTTTATTCGCGG	20	20	45.0	50.0	58.0	58.0	0.0	6.1	0.031586	53604	55216	2482-2482	0.250000	49.7
EI144_pgp15_p3	EI144_pgp15	3	ACGAGGTGGTTCACGAAAAA	TCCAAGTCTAATGTCGCGTG	20	20	45.0	50.0	58.0	58.0	0.0	5.8	0.036204	53604	55216	2142-2142	0.250000	49.7
EI144_pgp15_p4	EI144_pgp15	4	ACGAGGTGGTTCACGAAAAA	ACTCCTTTATTCGCGGGTTC	20	20	45.0	50.0	58.0	58.0	0.0	6.1	0.046951	53604	55216	2478-2478	0.250000	49.6
EI144_pgp15_p5	EI144_pgp15	5	ACGAGGTGGTTCACGAAAAA	GACTCCTTTATTCGCGGGTT	20	20	45.0	50.0	58.0	58.0	0.0	6.1	0.046951	53604	55216	2479-2479	0.250000	49.6
EI144_pgp08_p1	EI144_pgp08	1	CTTCAATCTGGCGTGACGTA	TCTAGCTTTTTGGCGCAAGA	20	20	50.0	45.0	58.0	58.1	6.9	10.8	0.118325	59250	60725	2682-2682	0.250000	49.1
EI144_pgp08_p2	EI144_pgp08	2	AATCTGGCGTGACGTAATCC	TCTAGCTTTTTGGCGCAAGA	20	20	50.0	45.0	58.1	58.1	6.9	10.8	0.173191	59250	60725	2678-2678	0.250000	48.6
EI144_pgp08_p3	EI144_pgp08	3	CTTCAATCTGGCGTGACGTA	GGTCGTTTAAATGGGGCAGA	20	20	50.0	50.0	58.0	58.2	6.9	7.6	0.180222	59250	60725	2298-2298	0.250000	48.6
EI144_pgp08_p4	EI144_pgp08	4	CTTCAATCTGGCGTGACGTA	TCTGGTCGTTTAAATGGGGC	20	20	50.0	50.0	58.0	58.2	6.9	7.6	0.180222	59250	60725	2301-2301	0.250000	48.6
EI144_pgp08_p5	EI144_pgp08	5	CGCGGCGAAACCAATAAAAT	TCTAGCTTTTTGGCGCAAGA	20	20	45.0	45.0	58.1	58.1	19.2	10.8	0.206284	59250	60725	2781-2781	0.250000	48.3