primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	ACGCTATCGATTGAACGCTT	ATTTCGAAAGGGGCAAGGTT	20	20	45.0	45.0	58.1	58.0	11.2	16.8	0.087111	1760	2427	914-949	0.750000	79.0
matK-rps16_p2	matK-rps16	2	TCGATTGAACGCTTTGTTGC	ATTTCGAAAGGGGCAAGGTT	20	20	45.0	45.0	58.0	58.0	0.0	16.8	0.040718	1760	2427	908-943	0.500000	66.8
matK-rps16_p3	matK-rps16	3	TCGATTGAACGCTTTGTTGC	AGTAGCTCAATCAACAGCGG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.046773	1760	2427	939-974	0.500000	66.8
matK-rps16_p4	matK-rps16	4	AGCTAGTTGACGCAGACAAG	ATTTCGAAAGGGGCAAGGTT	20	20	50.0	45.0	57.9	58.0	0.0	16.8	0.081370	1760	2427	1067-1091	0.500000	64.7
matK-rps16_p5	matK-rps16	5	AGCTAGTTGACGCAGACAAG	AGTAGCTCAATCAACAGCGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.087424	1760	2427	1122-1122	0.250000	49.3
rps16_p1	rps16	1	GCTTGCTTAGCCGCATATTG	ATGCGACGTATGCAGCTAAA	20	20	50.0	45.0	58.0	58.1	0.3	18.3	0.107515	2428	3489	1022-1531	0.500000	66.8
rps16_p2	rps16	2	CCCCTGCTTTTCCAACTTCT	ATGCGACGTATGCAGCTAAA	20	20	50.0	45.0	58.0	58.1	0.0	18.3	0.066487	2428	3489	1453-1453	0.250000	49.5
rps16_p3	rps16	3	CCCCTGCTTTTCCAACTTCT	TCTAGGTTACGAAGGCACCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.073589	2428	3489	1719-1719	0.250000	49.4
rps16_p4	rps16	4	CCCCTGCTTTTCCAACTTCT	GAGCGTCATATTGGCAAACG	20	20	50.0	50.0	58.0	57.9	0.0	15.7	0.092228	2428	3489	1811-1811	0.250000	49.3
rps16_p5	rps16	5	CCCCTGCTTTTCCAACTTCT	CCCTAGGATACCGACCCTTT	20	20	50.0	55.0	58.0	57.9	0.0	11.5	0.099694	2428	3489	1738-1738	0.250000	49.2
atpH-atpI_p1	atpH-atpI	1	TCCCTCCTGGAAAGTTTTGC	CATTCAGTTACCCCACGGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.028799	12349	13070	1690-1729	1.000000	87.9
atpH-atpI_p2	atpH-atpI	2	TCCCTCCTGGAAAGTTTTGC	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	0.0	4.9	0.004976	12349	13070	1882-1921	0.750000	75.2
atpH-atpI_p3	atpH-atpI	3	GAGGGAGTAGCGATCTCACT	AGGTGCCAGAAATCTGCAAA	20	20	55.0	45.0	58.0	58.0	9.6	4.9	0.024692	12349	13070	1921-1960	0.750000	75.0
atpH-atpI_p4	atpH-atpI	4	ACACCCCCTTTCCGTAGTAA	AGGTGCCAGAAATCTGCAAA	20	20	50.0	45.0	58.0	58.0	0.0	4.9	0.028913	12349	13070	2034-2073	0.750000	74.8
atpH-atpI_p5	atpH-atpI	5	TCCCTCCTGGAAAGTTTTGC	GCAAGTAGGGGTCTTCGAAG	20	20	50.0	55.0	58.0	58.0	0.0	19.4	0.016164	12349	13070	1973-1973	0.250000	49.9
rpoB-trnD-GUC_p1	rpoB-trnD-GUC	1	CAAGTTCTGGTAGTACGGGC	GAGTTCATCGGGATTGACGG	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.434052	25432	26136	773-809	1.000000	88.2
rpoB-trnD-GUC_p2	rpoB-trnD-GUC	2	CAAGTTCTGGTAGTACGGGC	GGAGTTCATCGGGATTGACG	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.434052	25432	26136	774-810	1.000000	88.2
rpoB-trnD-GUC_p3	rpoB-trnD-GUC	3	GCAAGTTCTGGTAGTACGGG	GAGTTCATCGGGATTGACGG	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.434052	25432	26136	774-810	1.000000	88.2
rpoB-trnD-GUC_p4	rpoB-trnD-GUC	4	GCAAGTTCTGGTAGTACGGG	GGAGTTCATCGGGATTGACG	20	20	55.0	55.0	58.0	58.4	0.0	0.0	0.434052	25432	26136	775-811	1.000000	88.2
rpoB-trnD-GUC_p5	rpoB-trnD-GUC	5	AGTTCTGGTAGTACGGGCAT	GAGTTCATCGGGATTGACGG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.566099	25432	26136	771-807	1.000000	87.1
petN-trnC-GCA_p1	petN-trnC-GCA	1	GAGCTCCCCCAGATAATTGC	TGCAGGCGAAGACCTATCTA	20	20	55.0	50.0	58.1	57.9	0.9	0.0	0.164427	28221	28677	1354-1379	0.750000	77.0
petN-trnC-GCA_p2	petN-trnC-GCA	2	GTTTTTCATTCCGGGTGCAG	TGCAGGCGAAGACCTATCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.131939	28221	28677	1562-1569	0.500000	64.3
petN-trnC-GCA_p3	petN-trnC-GCA	3	CTGTTTTTCATTCCGGGTGC	TGCAGGCGAAGACCTATCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.131939	28221	28677	1571-1571	0.250000	48.9
petN-trnC-GCA_p4	petN-trnC-GCA	4	GAGTTAACGGAGGTGTGACC	TGCAGGCGAAGACCTATCTA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.143344	28221	28677	1519-1519	0.250000	48.9
petN-trnC-GCA_p5	petN-trnC-GCA	5	TCCCTGTTTTTCATTCCGGG	TGCAGGCGAAGACCTATCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.149493	28221	28677	1574-1574	0.250000	48.8
trnC-GCA-trnG-GCC_p1	trnC-GCA-trnG-GCC	1	GATTTGCAGTCCTCCGTCTT	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	57.9	58.2	0.0	0.0	1.290119	28750	29234	540-563	1.000000	78.8
trnC-GCA-trnG-GCC_p2	trnC-GCA-trnG-GCC	2	GATTTGCAGTCCTCCGTCTT	GGGTTCGATTCCCGCTATC	20	19	50.0	57.9	57.9	57.8	0.0	9.9	1.313054	28750	29234	542-565	1.000000	78.7
trnC-GCA-trnG-GCC_p3	trnC-GCA-trnG-GCC	3	GATTTGCAGTCCTCCGTCTT	GGTTCGATTCCCGCTATCC	20	19	50.0	57.9	57.9	57.8	0.0	0.0	1.313054	28750	29234	541-564	1.000000	78.7
trnC-GCA-trnG-GCC_p4	trnC-GCA-trnG-GCC	4	TCCGTCTTACCGCTTGACTA	GTTCGATTCCCGCTATCCG	20	19	50.0	57.9	58.2	58.2	0.0	0.0	1.364424	28750	29234	528-551	1.000000	78.3
trnC-GCA-trnG-GCC_p5	trnC-GCA-trnG-GCC	5	TCCGTCTTACCGCTTGACTA	GGTTCGATTCCCGCTATCC	20	19	50.0	57.9	58.2	57.8	0.0	0.0	1.387359	28750	29234	529-552	1.000000	78.1
psbZ-trnS-UGA_p1	psbZ-trnS-UGA	1	AGCCAATTGGAATGCGATAGT	CCGCTCGACCATCTCTCC	21	18	42.9	66.7	58.1	59.3	29.0	0.0	4.338789	29772	30172	376-451	1.000000	57.3
psbZ-trnS-UGA_p2	psbZ-trnS-UGA	2	GCCAATTGGAATGCGATAGTC	CCGCTCGACCATCTCTCC	21	18	47.6	66.7	57.7	59.3	29.0	0.0	4.548910	29772	30172	375-450	1.000000	55.6
psbZ-trnS-UGA_p3	psbZ-trnS-UGA	3	AAAGCCAATTGGAATGCGAT	CCGCTCGACCATCTCTCC	20	18	40.0	66.7	56.6	59.3	29.0	0.0	4.630532	29772	30172	378-453	1.000000	55.0
psbZ-trnS-UGA_p4	psbZ-trnS-UGA	4	GCCAATTGGAATGCGATAGT	CCGCTCGACCATCTCTCC	20	18	45.0	66.7	56.5	59.3	29.0	0.0	4.726038	29772	30172	375-450	1.000000	54.2
psbZ-trnS-UGA_p5	psbZ-trnS-UGA	5	AGCCAATTGGAATGCGATAG	CCGCTCGACCATCTCTCC	20	18	45.0	66.7	56.3	59.3	29.0	0.0	5.022503	29772	30172	376-451	1.000000	52.0
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	TTTTCCGATGGCAATGGTCA	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.536534	33003	34395	1478-1503	1.000000	85.2
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TTTCCGATGGCAATGGTCAT	AACTCAGGGGTAGAGTGACG	20	20	45.0	55.0	57.9	58.5	8.1	0.0	0.595396	33003	34395	1477-1502	1.000000	84.7
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	TTTTCCGATGGCAATGGTCA	GCCATGGTAAGGCGTAAGTC	20	20	45.0	55.0	58.1	58.7	8.1	14.7	0.786767	33003	34395	1459-1484	1.000000	83.2
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	TTTCCGATGGCAATGGTCAT	GCCATGGTAAGGCGTAAGTC	20	20	45.0	55.0	57.9	58.7	8.1	14.7	0.845629	33003	34395	1458-1483	1.000000	82.7
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	TTTTCCGATGGCAATGGTCA	GGTTCAAATCCGACAAGGGG	20	20	45.0	55.0	58.1	58.8	8.1	0.0	0.909582	33003	34395	1436-1461	1.000000	82.2
trnT-GGU-trnfM-CAU_p1	trnT-GGU-trnfM-CAU	1	TTAGCCCCTTGTCGGATTTG	CAGGTTCAAATCCTGTCCCC	20	20	50.0	55.0	57.9	58.2	0.0	4.1	0.283360	34468	34946	573-583	1.000000	86.6
trnT-GGU-trnfM-CAU_p2	trnT-GGU-trnfM-CAU	2	TTAGCCCCTTGTCGGATTTG	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.347852	34468	34946	604-614	1.000000	86.0
trnT-GGU-trnfM-CAU_p3	trnT-GGU-trnfM-CAU	3	TTAGCCCCTTGTCGGATTTG	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.347852	34468	34946	603-613	1.000000	86.0
trnT-GGU-trnfM-CAU_p4	trnT-GGU-trnfM-CAU	4	TTAGCCCCTTGTCGGATTTG	TGTTGGGGGCATCAACTAAG	20	20	50.0	50.0	57.9	57.8	0.0	18.3	0.337159	34468	34946	641-651	1.000000	85.9
trnT-GGU-trnfM-CAU_p5	trnT-GGU-trnfM-CAU	5	ACGACTTACGCCTTACCATG	CAGGTTCAAATCCTGTCCCC	20	20	50.0	55.0	57.7	58.2	0.0	4.1	0.449537	34468	34946	548-558	1.000000	85.3
trnR-UCG-accD_p1	trnR-UCG-accD	1	GTTCGAATCCCCACCAACTC	GGTAGCTTCTTCGGGGAAAG	20	20	55.0	55.0	58.5	58.0	0.0	0.0	0.575563	53245	53564	416-432	1.000000	87.1
trnR-UCG-accD_p2	trnR-UCG-accD	2	GTTCGAATCCCCACCAACTC	TCAATCCACCAAATTTGCGC	20	20	55.0	45.0	58.5	57.9	0.0	12.9	0.627388	53245	53564	390-406	1.000000	87.0
trnR-UCG-accD_p3	trnR-UCG-accD	3	GTTCGAATCCCCACCAACTC	ATCCACCAAATTTGCGCCTA	20	20	55.0	45.0	58.5	58.2	0.0	12.9	0.703968	53245	53564	387-403	1.000000	86.4
trnR-UCG-accD_p4	trnR-UCG-accD	4	GTTCGAATCCCCACCAACTC	TCCACCAAATTTGCGCCTAT	20	20	55.0	45.0	58.5	58.2	0.0	12.9	0.703968	53245	53564	386-402	1.000000	86.4
trnR-UCG-accD_p5	trnR-UCG-accD	5	GTTCGAATCCCCACCAACTC	TGCGCCTATCTTCAAACCAA	20	20	55.0	45.0	58.5	57.8	0.0	4.2	0.742638	53245	53564	375-391	1.000000	86.1
rrn16-rps12_p1	rrn16-rps12	1	ACTGCCGAGTCATGTCAATC	AGTACTTCCCCCAGTCGAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035543	93092	93241	531-531	0.250000	49.7
rrn16-rps12_p2	rrn16-rps12	2	ACTGCCGAGTCATGTCAATC	CGGAGTATTTGGCAAGCTCT	20	20	50.0	50.0	58.0	58.0	0.0	20.0	0.045574	93092	93241	649-649	0.250000	49.6
rrn16-rps12_p3	rrn16-rps12	3	ACTGCCGAGTCATGTCAATC	GAAGGTCGTCGAGTCTTGAC	20	20	50.0	55.0	58.0	58.0	0.0	13.5	0.049314	93092	93241	607-607	0.250000	49.6
rrn16-rps12_p4	rrn16-rps12	4	ACTGCCGAGTCATGTCAATC	GACTTGAAGGTCGTCGAGTC	20	20	50.0	55.0	58.0	58.0	0.0	15.0	0.049314	93092	93241	612-612	0.250000	49.6
rrn16-rps12_p5	rrn16-rps12	5	ATACGCCTAGAGTGACGTCA	AGTACTTCCCCCAGTCGAAA	20	20	50.0	50.0	58.0	58.0	15.8	0.0	0.053912	93092	93241	469-469	0.250000	49.6
rps12-rrn16_p1	rps12-rrn16	1	AGTACTTCCCCCAGTCGAAA	ACTGCCGAGTCATGTCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035543	144851	145000	531-531	0.250000	49.7
rps12-rrn16_p2	rps12-rrn16	2	CGGAGTATTTGGCAAGCTCT	ACTGCCGAGTCATGTCAATC	20	20	50.0	50.0	58.0	58.0	20.0	0.0	0.045574	144851	145000	649-649	0.250000	49.6
rps12-rrn16_p3	rps12-rrn16	3	GAAGGTCGTCGAGTCTTGAC	ACTGCCGAGTCATGTCAATC	20	20	55.0	50.0	58.0	58.0	13.5	0.0	0.049314	144851	145000	607-607	0.250000	49.6
rps12-rrn16_p4	rps12-rrn16	4	GACTTGAAGGTCGTCGAGTC	ACTGCCGAGTCATGTCAATC	20	20	55.0	50.0	58.0	58.0	15.0	0.0	0.049314	144851	145000	612-612	0.250000	49.6
rps12-rrn16_p5	rps12-rrn16	5	AGTACTTCCCCCAGTCGAAA	ATACGCCTAGAGTGACGTCA	20	20	50.0	50.0	58.0	58.0	0.0	15.8	0.053912	144851	145000	469-469	0.250000	49.6