primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4384	5195	937-949	1.000000	77.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4384	5195	936-948	1.000000	77.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4384	5195	1021-1033	1.000000	76.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.554139	4384	5195	1020-1032	1.000000	76.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.3	58.3	0.0	7.6	0.579483	4384	5195	952-964	1.000000	76.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6305	7256	1009-1025	1.000000	80.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6305	7256	1024-1040	1.000000	78.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6305	7256	1023-1039	1.000000	78.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CCAAGAGCACCTTCATTCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.579996	6305	7256	1080-1096	1.000000	77.4
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6305	7256	1013-1029	1.000000	75.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACCAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	21	20	42.9	50.0	57.5	58.3	0.0	0.0	1.713302	10918	11564	766-766	1.000000	66.8
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGTAGAAAAGCGGTAGGAACC	TTCAATAGGCAAGAGCACCG	21	20	47.6	50.0	57.4	58.3	0.0	0.0	1.868756	10918	11564	781-781	1.000000	65.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGAACCAAAATGAACAGTGC	TTCAATAGGCAAGAGCACCG	21	20	42.9	50.0	57.3	58.3	0.0	0.0	1.995605	10918	11564	767-767	1.000000	64.6
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	ACCAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	56.1	58.3	0.0	0.0	2.201015	10918	11564	763-763	1.000000	62.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GAACCAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	22	20	40.9	50.0	58.0	58.3	0.0	0.0	2.307798	10918	11564	765-765	1.000000	62.1
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.0	56.8	0.0	0.0	2.237066	12015	12951	1078-1080	1.000000	62.8
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.1	56.8	14.8	0.0	2.326325	12015	12951	1037-1039	1.000000	62.1
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	12015	12951	1068-1070	1.000000	61.9
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	12015	12951	1073-1075	1.000000	61.9
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.0	57.6	0.0	0.0	2.409970	12015	12951	1068-1070	1.000000	61.4
atpA_p1	atpA	1	TTGGCATATTGGGGACGATG	ATCCGAAACGCTTGAGTACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026116	28194	29720	1688-1690	1.000000	79.9
atpA_p2	atpA	2	TTGGCATATTGGGGACGATG	TGAATCCGAAACGCTTGAGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.183963	28194	29720	1691-1693	1.000000	78.6
atpA_p3	atpA	3	TTGGCATATTGGGGACGATG	CGAAACGCTTGAGTACCCTT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.228140	28194	29720	1685-1687	1.000000	78.3
atpA_p4	atpA	4	GGCATATTGGGGACGATGAA	ATCCGAAACGCTTGAGTACC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.276323	28194	29720	1686-1688	1.000000	77.9
atpA_p5	atpA	5	GGCATATTGGGGACGATGAA	TGAATCCGAAACGCTTGAGT	20	20	50.0	45.0	57.7	57.8	0.0	0.0	0.434170	28194	29720	1689-1691	1.000000	76.6
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	GAACGAAACAAAACGCGGAT	GGGTAGAGCAGTTTGGTAGC	20	20	45.0	55.0	58.0	58.0	12.5	0.0	0.052503	35945	36147	358-376	1.000000	83.8
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAACGAAACAAAACGCGGAT	CTTGAGGTCACGGGTTCAAA	20	20	45.0	50.0	58.0	58.0	12.5	4.2	0.065121	35945	36147	322-340	1.000000	83.7
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	GAACGAAACAAAACGCGGAT	TTCAGTTCTTCTCTTCGGCG	20	20	45.0	50.0	58.0	57.9	12.5	0.0	0.092908	35945	36147	380-398	1.000000	83.5
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	GAACGAAACAAAACGCGGAT	GTCACGGGTTCAAATCCTGT	20	20	45.0	50.0	58.0	58.1	12.5	0.0	0.134705	35945	36147	316-334	1.000000	83.2
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	TCTTTGCCAAGGAGAAGACG	GGGTAGAGCAGTTTGGTAGC	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.195421	35945	36147	317-335	1.000000	82.7
ndhC-trnC-ACA_p1	ndhC-trnC-ACA	1	CGGTCCTTTGTTACTCGGAG	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.0	58.1	2.8	0.0	0.115363	50051	51209	1281-1309	1.000000	81.5
ndhC-trnC-ACA_p2	ndhC-trnC-ACA	2	GGTCCTTTGTTACTCGGAGC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.3	58.1	4.6	0.0	0.384313	50051	51209	1280-1308	1.000000	79.3
ndhC-trnC-ACA_p3	ndhC-trnC-ACA	3	ACTCGGAGCTAAAAATCCCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.7	58.1	4.6	0.0	0.418656	50051	51209	1269-1297	1.000000	79.1
ndhC-trnC-ACA_p4	ndhC-trnC-ACA	4	TTCCGGTCCTTTGTTACTCG	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.6	58.1	0.0	0.0	0.551420	50051	51209	1284-1312	1.000000	78.0
ndhC-trnC-ACA_p5	ndhC-trnC-ACA	5	CGGTCCTTTGTTACTCGGAG	GTTCGAGTCCGTATAGCCCT	20	20	55.0	55.0	58.0	58.7	2.8	0.0	0.691179	50051	51209	1266-1294	1.000000	76.9
accD_p1	accD	1	TTTTCAATACTCACACTCTTTTT	TGTCAACCCCATTCGTCTAA	23	20	26.1	45.0	52.8	56.5	0.0	0.0	9.748806	56927	58366	1609-1623	1.000000	41.2
accD_p2	accD	2	TTTTTCAATACTCACACTCTTTT	TGTCAACCCCATTCGTCTAA	23	20	26.1	45.0	52.8	56.5	0.0	0.0	9.748806	56927	58366	1610-1624	1.000000	41.2
accD_p3	accD	3	TTTTTCAATACTCACACTCTTTTT	TGTCAACCCCATTCGTCTAA	24	20	25.0	45.0	53.6	56.5	0.0	0.0	9.934798	56927	58366	1610-1624	1.000000	41.2
accD_p4	accD	4	TTTTCAATACTCACACTCTTTTT	ATGTCAACCCCATTCGTCTA	23	20	26.1	45.0	52.8	56.2	0.0	0.0	9.982287	56927	58366	1610-1624	1.000000	41.2
accD_p5	accD	5	TTTTCAATACTCACACTCTTTTT	TATGTCAACCCCATTCGTCT	23	20	26.1	45.0	52.8	56.2	0.0	0.0	9.982287	56927	58366	1611-1625	1.000000	41.2
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	60116	60867	932-934	1.000000	80.1
ycf4-cemA_p2	ycf4-cemA	2	GAGCCATTCCATTGACTCGT	CCACCAATGAGTAACCCAGG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.158609	60116	60867	976-978	1.000000	79.6
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	60116	60867	896-898	1.000000	79.6
ycf4-cemA_p4	ycf4-cemA	4	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	60116	60867	864-866	1.000000	79.6
ycf4-cemA_p5	ycf4-cemA	5	TTGGCCTATTTCTTGCGTGT	CCACCAATGAGTAACCCAGG	20	20	45.0	55.0	58.1	57.9	3.3	0.0	0.224484	60116	60867	908-910	1.000000	79.2
clpP1_p1	clpP1	1	AAGTCGGCAGGGGTAATTTC	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	69566	71573	2125-2133	1.000000	70.3
clpP1_p2	clpP1	2	GAAAGTCGGCAGGGGTAATT	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.263647	69566	71573	2127-2135	1.000000	70.3
clpP1_p3	clpP1	3	TATGAAAGTCGGCAGGGGTA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.1	56.9	0.0	0.0	1.270657	69566	71573	2130-2138	1.000000	70.2
clpP1_p4	clpP1	4	GTCGGCAGGGGTAATTTCAA	GGCTTTGTTACGTTTCCACA	20	20	50.0	45.0	58.2	56.9	0.0	0.0	1.310391	69566	71573	2123-2131	1.000000	69.9
clpP1_p5	clpP1	5	AAGTCGGCAGGGGTAATTTC	AGGCTTTGTTACGTTTCCACA	20	21	50.0	42.9	57.9	58.4	0.0	0.0	1.478475	69566	71573	2126-2134	1.000000	68.6
psbB_p1	psbB	1	ATCCACTAAAGAGGGGGTCC	TCCCGGATAAAGAAAGAGCA	20	20	55.0	45.0	58.1	56.2	0.0	0.7	1.865889	72029	73555	1724-1736	1.000000	65.8
psbB_p2	psbB	2	ATCCACTAAAGAGGGGGTCC	TCCCGGATAAAGAAAGAGCAA	20	21	55.0	42.9	58.1	57.0	0.0	0.7	2.098244	72029	73555	1724-1736	1.000000	64.0
psbB_p3	psbB	3	CCACTAAAGAGGGGGTCCAT	TCCCGGATAAAGAAAGAGCA	20	20	55.0	45.0	58.4	56.2	0.0	0.7	2.164093	72029	73555	1722-1734	1.000000	63.4
psbB_p4	psbB	4	ATCCACTAAAGAGGGGGTCC	CCCGGATAAAGAAAGAGCAAGA	20	22	55.0	45.5	58.1	58.1	0.0	0.7	2.231391	72029	73555	1723-1735	1.000000	62.9
psbB_p5	psbB	5	ATCCACTAAAGAGGGGGTCC	TCCCGGATAAAGAAAGAGCAAG	20	22	55.0	45.5	58.1	58.1	0.0	0.7	2.231391	72029	73555	1724-1736	1.000000	62.9
petB_p1	petB	1	CCCCCAAGTCCTAGCTTTTC	ATCGTTCCTACCCCAAGTGA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.183288	74484	75880	1565-1575	1.000000	79.5
petB_p2	petB	2	CCCCCAAGTCCTAGCTTTTC	TCGTTCCTACCCCAAGTGAT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.183288	74484	75880	1564-1574	1.000000	79.5
petB_p3	petB	3	TTTTGGTAGTTCGACCGTGG	ATCGTTCCTACCCCAAGTGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.184546	74484	75880	1509-1513	1.000000	78.8
petB_p4	petB	4	TTTTGGTAGTTCGACCGTGG	TCGTTCCTACCCCAAGTGAT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.184546	74484	75880	1508-1512	1.000000	78.8
petB_p5	petB	5	GGTAGTTCGACCGTGGAATT	TCGTTCCTACCCCAAGTGAT	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.428189	74484	75880	1504-1508	1.000000	76.8
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	ACAAAGCTTTTAACGCTGCC	20	20	50.0	45.0	58.0	57.9	17.5	9.5	0.166615	110880	111919	1218-1235	1.000000	80.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	ACAAAGCTTTTAACGCTGCC	20	20	45.0	45.0	58.0	57.9	17.5	9.5	0.177905	110880	111919	1216-1233	1.000000	80.6
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	ACAAAGCTTTTAACGCTGCC	20	20	45.0	45.0	58.0	57.9	17.5	9.5	0.177905	110880	111919	1217-1234	1.000000	80.6
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	GCCCAATACCCCTTCCTTTT	20	20	50.0	50.0	58.0	57.8	17.5	0.0	0.280113	110880	111919	1201-1218	1.000000	79.8
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	GCCCAATACCCCTTCCTTTT	20	20	45.0	50.0	58.0	57.8	17.5	0.0	0.291402	110880	111919	1199-1216	1.000000	79.7
ndhH_p1	ndhH	1	GCATCCATATGATCCCATAGACC	CGCTTTCAGCAATTCATGGA	23	20	47.8	45.0	58.3	57.1	3.7	0.0	4.213102	120582	121763	1312-1312	1.000000	46.5
ndhH_p2	ndhH	2	GCATCCATATGATCCCATAGACC	TCGCTTTCAGCAATTCATGG	23	20	47.8	45.0	58.3	57.1	3.7	0.0	4.213102	120582	121763	1313-1313	1.000000	46.5
ndhH_p3	ndhH	3	TCCATATGATCCCATAGACCTCT	CGCTTTCAGCAATTCATGGA	23	20	43.5	45.0	57.4	57.1	3.7	0.0	4.484434	120582	121763	1309-1309	1.000000	44.3
ndhH_p4	ndhH	4	TCCATATGATCCCATAGACCTCT	TCGCTTTCAGCAATTCATGG	23	20	43.5	45.0	57.4	57.1	3.7	0.0	4.484434	120582	121763	1310-1310	1.000000	44.3
ndhH_p5	ndhH	5	GCATCCATATGATCCCATAGACC	CGCTTTCAGCAATTCATGGAA	23	21	47.8	42.9	58.3	57.8	3.7	0.0	4.508032	120582	121763	1312-1312	1.000000	44.1
rps15-ycf1_p1	rps15-ycf1	1	ACTTGAAATTCAACAGATCCTCT	CGCTTGAGTATGTTAAGGAT	23	20	34.8	40.0	55.8	52.8	4.0	0.0	10.338209	122133	122531	518-523	1.000000	41.1
rps15-ycf1_p2	rps15-ycf1	2	CAGATCCTCTGTTTTCTTTGT	CGCTTGAGTATGTTAAGGAT	21	20	38.1	40.0	53.7	52.8	0.0	0.0	10.434449	122133	122531	505-510	1.000000	41.1
rps15-ycf1_p3	rps15-ycf1	3	AGTCTCCGTATCTTATTGGT	CGCTTGAGTATGTTAAGGAT	20	20	40.0	40.0	52.9	52.8	0.0	0.0	10.327644	122133	122531	548-553	1.000000	41.0
rps15-ycf1_p4	rps15-ycf1	4	AAGTCTCCGTATCTTATTGGT	CGCTTGAGTATGTTAAGGAT	21	20	38.1	40.0	53.8	52.8	0.0	0.0	10.394639	122133	122531	549-554	1.000000	41.0
rps15-ycf1_p5	rps15-ycf1	5	AGTAAGTCTCCGTATCTTATTGGT	CGCTTGAGTATGTTAAGGAT	24	20	37.5	40.0	56.7	52.8	0.0	0.0	10.466162	122133	122531	552-557	1.000000	41.0
ycf1_p1	ycf1	1	GGTCTTCTTCTTCGCCCATT	ACATTGGATCTAAGAAGTCCC	20	21	50.0	42.9	57.9	54.7	0.0	0.0	4.414757	126782	126901	171-204	1.000000	47.2
ycf1_p2	ycf1	2	GGTCTTCTTCTTCGCCCATT	AGTACATTGGATCTAAGAAGTCCC	20	24	50.0	41.7	57.9	57.5	0.0	0.0	4.602642	126782	126901	174-207	1.000000	45.7
ycf1_p3	ycf1	3	TCAAGGTCTTCTTCTTCGCC	ACATTGGATCTAAGAAGTCCC	20	21	50.0	42.9	57.5	54.7	0.0	0.0	4.756780	126782	126901	175-208	1.000000	44.5
ycf1_p4	ycf1	4	CAAGGTCTTCTTCTTCGCCC	ACATTGGATCTAAGAAGTCCC	20	21	55.0	42.9	58.6	54.7	0.0	0.0	4.855613	126782	126901	174-207	1.000000	43.7
ycf1_p5	ycf1	5	TCAAGGTCTTCTTCTTCGCC	AGTACATTGGATCTAAGAAGTCCC	20	24	50.0	41.7	57.5	57.5	0.0	0.0	4.944665	126782	126901	178-211	1.000000	42.9