primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	20	20	50.0	55.0	58.4	57.9	13.2	7.8	0.538239	51590	51815	334-348	1.000000	77.8
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	20	20	50.0	55.0	58.4	58.1	13.2	0.0	0.551943	51590	51815	336-350	1.000000	77.7
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.558762	51590	51815	338-352	1.000000	77.6
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TATTGCTTTCATACGGCGGG	AGGCAAATCTAGCTCTCCGA	20	20	50.0	50.0	58.4	58.2	13.2	0.0	0.629540	51590	51815	337-351	1.000000	77.1
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TATTGCTTTCATACGGCGGG	CTAGGACACGAGTAGAGGCT	20	20	50.0	55.0	58.4	57.7	13.2	0.0	0.746642	51590	51815	313-327	1.000000	76.3
ndhF_p1	ndhF	1	CTAGCACTGGGTAGACCTCA	CGAAAGAGGTAAGATCCGTTGA	20	22	55.0	45.5	57.9	57.9	0.0	0.0	2.251426	108083	110317	2594-2594	0.500000	42.0
ndhF_p2	ndhF	2	CTAGCACTGGGTAGACCTCA	TCGAAAGAGGTAAGATCCGTTG	20	22	55.0	45.5	57.9	57.9	0.0	0.0	2.251426	108083	110317	2595-2595	0.500000	42.0
ndhF_p3	ndhF	3	ATCAATTCGGTCGTTGTGGT	CGAAAGAGGTAAGATCCGTTGA	20	22	45.0	45.5	57.8	57.9	0.0	0.0	2.284941	108083	110317	2792-2792	0.500000	41.7
ndhF_p4	ndhF	4	ATCAATTCGGTCGTTGTGGT	TCGAAAGAGGTAAGATCCGTTG	20	22	45.0	45.5	57.8	57.9	0.0	0.0	2.284941	108083	110317	2793-2793	0.500000	41.7
ndhF_p5	ndhF	5	TCTCTTCCTTCTCCGAGCTC	CGAAAGAGGTAAGATCCGTTGA	20	22	55.0	45.5	58.2	57.9	2.7	0.0	2.348346	108083	110317	2724-2724	0.500000	41.2
ndhF-rpl32_p1	ndhF-rpl32	1	AAGGGGAAATTGTGCGGATT	ACGCCGACCAATATCCTTTC	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.113344	110318	111355	1861-1861	0.500000	59.1
ndhF-rpl32_p2	ndhF-rpl32	2	AGGGGAAATTGTGCGGATTT	ACGCCGACCAATATCCTTTC	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.113344	110318	111355	1860-1860	0.500000	59.1
ndhF-rpl32_p3	ndhF-rpl32	3	GGGAAATTGTGCGGATTTCG	ACGCCGACCAATATCCTTTC	20	20	50.0	50.0	58.1	58.1	11.0	0.0	0.142469	110318	111355	1858-1858	0.500000	58.9
ndhF-rpl32_p4	ndhF-rpl32	4	AACAGGTCGTGTGAACCAAA	ACGCCGACCAATATCCTTTC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.167362	110318	111355	1633-1633	0.500000	58.7
ndhF-rpl32_p5	ndhF-rpl32	5	ACAGGTCGTGTGAACCAAAA	ACGCCGACCAATATCCTTTC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.167362	110318	111355	1632-1632	0.500000	58.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAAAGGATATTGGTCGGCGT	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.1	57.9	6.8	0.0	0.149676	111521	112048	1267-1267	0.500000	58.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAAAGGATATTGGTCGGCGT	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.1	57.9	6.8	0.0	0.174812	111521	112048	667-667	0.500000	58.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAAAGGATATTGGTCGGCGT	AGCAGCGTGTCTACCAATTT	20	20	50.0	45.0	58.1	57.8	6.8	0.0	0.235539	111521	112048	660-660	0.500000	58.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAAAGGATATTGGTCGGCGT	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.1	57.8	6.8	0.0	0.246830	111521	112048	666-666	0.500000	58.0
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAAAGGATATTGGTCGGCGT	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.1	57.8	6.8	0.0	0.246830	111521	112048	665-665	0.500000	58.0
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GAAAGGATATTGGTCGGCGT	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.1	57.9	6.8	0.0	0.149676	112129	112225	1267-1267	0.500000	58.8
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TAGACACGCTGCTCTTAGGA	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.223418	112129	112225	620-620	0.500000	58.2
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GAAAGGATATTGGTCGGCGT	TAGCCCATACTGCTCCTGAA	20	20	50.0	50.0	58.1	57.8	6.8	0.0	0.225220	112129	112225	1541-1541	0.500000	58.2
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GAAAGGATATTGGTCGGCGT	TTAGCCCATACTGCTCCTGA	20	20	50.0	50.0	58.1	57.8	6.8	0.0	0.225220	112129	112225	1542-1542	0.500000	58.2
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AAATTGGTAGACACGCTGCT	TGATCCACAAAGAAGAGCCG	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.284145	112129	112225	627-627	0.500000	57.7
ccsA_p1	ccsA	1	TAGACACGCTGCTCTTAGGA	AGAACCGCGTGAATCACTAC	20	20	50.0	50.0	57.9	58.0	0.0	11.9	0.132107	112226	113194	1236-1236	0.500000	58.9
ccsA_p2	ccsA	2	AAATTGGTAGACACGCTGCT	AGAACCGCGTGAATCACTAC	20	20	45.0	50.0	57.8	58.0	0.0	11.9	0.192834	112226	113194	1243-1243	0.500000	58.5
ccsA_p3	ccsA	3	GGTAGACACGCTGCTCTTAG	AGAACCGCGTGAATCACTAC	20	20	55.0	50.0	57.8	58.0	0.0	11.9	0.204125	112226	113194	1238-1238	0.500000	58.4
ccsA_p4	ccsA	4	GTAGACACGCTGCTCTTAGG	AGAACCGCGTGAATCACTAC	20	20	55.0	50.0	57.8	58.0	0.0	11.9	0.204125	112226	113194	1237-1237	0.500000	58.4
ccsA_p5	ccsA	5	TAGACACGCTGCTCTTAGGA	GCATTACCGGGTATGAGTGG	20	20	50.0	55.0	57.9	58.1	0.0	18.9	0.245324	112226	113194	1725-1725	0.500000	58.0
ndhD_p1	ndhD	1	GTAGTGATTCACGCGGTTCT	ATTTTGGAGCACGGGTTTCT	20	20	50.0	45.0	58.0	58.0	12.5	0.0	0.012454	113448	114950	1714-1714	0.500000	59.9
ndhD_p2	ndhD	2	GTAGTGATTCACGCGGTTCT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.0	58.0	12.5	0.0	0.023287	113448	114950	1912-1912	0.500000	59.8
ndhD_p3	ndhD	3	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	12.5	0.0	0.102592	113448	114950	2132-2132	0.500000	59.2
ndhD_p4	ndhD	4	GTAGTGATTCACGCGGTTCT	GGGTTTCTCTGGTCCAAGTG	20	20	50.0	55.0	58.0	58.1	12.5	0.0	0.110270	113448	114950	1702-1702	0.500000	59.1
ndhD_p5	ndhD	5	GTAGTGATTCACGCGGTTCT	TGATACCTTGGGACGGATGT	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.133701	113448	114950	1950-1950	0.500000	58.9
psaC_p1	psaC	1	AGAAACCCGTGCTCCAAAAT	AAAACTCGGGGAACTCATCG	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.099386	115070	115315	438-438	0.500000	59.2
psaC_p2	psaC	2	CTCCCGCCGTGTACAAAATA	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107699	115070	115315	985-985	0.500000	59.1
psaC_p3	psaC	3	TCTTTCCTCCCCACATGGAT	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.109141	115070	115315	940-940	0.500000	59.1
psaC_p4	psaC	4	AGAAACCCGTGCTCCAAAAT	CTCGGGGAACTCATCGATTC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.146159	115070	115315	434-434	0.500000	58.8
psaC_p5	psaC	5	CTCCCGCCGTGTACAAAATA	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.154472	115070	115315	981-981	0.500000	58.8
psaC-ndhE_p1	psaC-ndhE	1	AGAAACCCGTGCTCCAAAAT	TTAATCGGGGCAATTGCTGT	20	20	45.0	45.0	58.0	58.2	0.0	28.4	0.158617	115316	115549	1129-1129	0.500000	58.7
psaC-ndhE_p2	psaC-ndhE	2	TCCTCTGTTCTTGGAGCAGA	TTAATCGGGGCAATTGCTGT	20	20	50.0	45.0	58.0	58.2	12.1	28.4	0.169450	115316	115549	931-931	0.500000	58.6
psaC-ndhE_p3	psaC-ndhE	3	AGAAACCCGTGCTCCAAAAT	GTTAATCGGGGCAATTGCTG	20	20	45.0	50.0	58.0	57.8	0.0	28.4	0.212950	115316	115549	1130-1130	0.500000	58.3
psaC-ndhE_p4	psaC-ndhE	4	TCCTCTGTTCTTGGAGCAGA	GTTAATCGGGGCAATTGCTG	20	20	50.0	50.0	58.0	57.8	12.1	28.4	0.223782	115316	115549	932-932	0.500000	58.2
psaC-ndhE_p5	psaC-ndhE	5	CGGACACTCAAGAAATCCGT	TTAATCGGGGCAATTGCTGT	20	20	50.0	45.0	57.9	58.2	2.9	28.4	0.234279	115316	115549	988-988	0.500000	58.1
ndhG_p1	ndhG	1	CGATGAGTTCCCCGAGTTTT	TCAAATTGCTTTGGGTCGGT	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.396256	116074	116604	1648-1648	0.500000	56.8
ndhG_p2	ndhG	2	GAATCGATGAGTTCCCCGAG	TCAAATTGCTTTGGGTCGGT	20	20	55.0	45.0	57.9	58.3	0.0	0.0	0.443029	116074	116604	1652-1652	0.500000	56.5
ndhG_p3	ndhG	3	CTTCTGGATTGACAGTGCCA	TCAAATTGCTTTGGGTCGGT	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.491088	116074	116604	1616-1616	0.500000	56.1
ndhG_p4	ndhG	4	ACTTCTGGATTGACAGTGCC	TCAAATTGCTTTGGGTCGGT	20	20	50.0	45.0	57.8	58.3	0.0	0.0	0.492622	116074	116604	1617-1617	0.500000	56.1
ndhG_p5	ndhG	5	CGATGAGTTCCCCGAGTTTT	TAGGAGGCGAGAATGGTGTT	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.526258	116074	116604	1480-1480	0.500000	55.8
ndhG-ndhI_p1	ndhG-ndhI	1	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064045	116605	116955	1359-1359	0.500000	59.5
ndhG-ndhI_p2	ndhG-ndhI	2	TAATACTACTCCCAGGCCCG	GATGGACTTTACCGAGGCTG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.064045	116605	116955	1133-1133	0.500000	59.5
ndhG-ndhI_p3	ndhG-ndhI	3	AGACTTGTACAGACCACGGA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.067554	116605	116955	1369-1369	0.500000	59.5
ndhG-ndhI_p4	ndhG-ndhI	4	TCCCAGGCCCGCTAATATAA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.091640	116605	116955	1124-1124	0.500000	59.3
ndhG-ndhI_p5	ndhG-ndhI	5	TAATACTACTCCCAGGCCCG	AGATGGACTTTACCGAGGCT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.138007	116605	116955	1134-1134	0.500000	58.9
ndhI_p1	ndhI	1	AGACCACGGAAGTTACTCCA	AGCCGAGTGTGAACGATTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	116956	117456	1664-1664	0.500000	59.9
ndhI_p2	ndhI	2	TAATACTACTCCCAGGCCCG	AGCCGAGTGTGAACGATTAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.013201	116956	117456	1438-1438	0.500000	59.9
ndhI_p3	ndhI	3	AGACTTGTACAGACCACGGA	AGCCGAGTGTGAACGATTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016709	116956	117456	1674-1674	0.500000	59.9
ndhI_p4	ndhI	4	AGACCACGGAAGTTACTCCA	TTTGTGACCTTTGAGGAGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.1	0.024114	116956	117456	1870-1870	0.500000	59.8
ndhI_p5	ndhI	5	TAATACTACTCCCAGGCCCG	TTTGTGACCTTTGAGGAGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.1	0.024114	116956	117456	1644-1644	0.500000	59.8
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	GTCGCTGCTGATTTACCTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048352	117535	119672	2984-2984	0.500000	59.6
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	117535	119672	2646-2646	0.500000	59.4
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	TTCAACAGGCCCTAGAAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	117535	119672	2647-2647	0.500000	59.4
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	117535	119672	2719-2719	0.500000	59.2
ndhA_p5	ndhA	5	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	117535	119672	2720-2720	0.500000	59.2
ndhH_p1	ndhH	1	TGCGTATTCAGGGCCAATAC	CGCTGTCTTACATCCCAGTC	20	20	50.0	55.0	58.0	58.1	6.2	0.0	0.110312	119674	120855	2037-2037	0.500000	59.1
ndhH_p2	ndhH	2	GTTGTTGTATCCCCGCAGAT	CGCTGTCTTACATCCCAGTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.110492	119674	120855	2017-2017	0.500000	59.1
ndhH_p3	ndhH	3	ATACGTTGTTGTATCCCCGC	CGCTGTCTTACATCCCAGTC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.128074	119674	120855	2021-2021	0.500000	59.0
ndhH_p4	ndhH	4	CCTGACATAAGAAGGCCGAC	CGCTGTCTTACATCCCAGTC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.130780	119674	120855	2282-2282	0.500000	59.0
ndhH_p5	ndhH	5	GACATAAGAAGGCCGACAGG	CGCTGTCTTACATCCCAGTC	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.130780	119674	120855	2279-2279	0.500000	59.0
rps15_p1	rps15	1	TGAGGTAAATCAGCAGCGAC	CGCTGTCTTACATCCCAGTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.082250	120947	121225	1159-1159	0.500000	59.3
rps15_p2	rps15	2	GAAAGGAGTCTGTGCACCAA	CGCTGTCTTACATCCCAGTC	20	20	50.0	55.0	58.0	58.1	15.7	0.0	0.104786	120947	121225	1046-1046	0.500000	59.2
rps15_p3	rps15	3	TCTGTGCACCAATATCTGCC	CGCTGTCTTACATCCCAGTC	20	20	50.0	55.0	58.0	58.1	15.7	0.0	0.114791	120947	121225	1038-1038	0.500000	59.1
rps15_p4	rps15	4	TGAGGTAAATCAGCAGCGAC	GGGAGCCGTTTGAGTATGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.119570	120947	121225	1319-1319	0.500000	59.0
rps15_p5	rps15	5	AACACCATGCATTGATGGGT	CGCTGTCTTACATCCCAGTC	20	20	45.0	55.0	58.1	58.1	8.9	0.0	0.130728	120947	121225	728-728	0.500000	59.0
rps15-ycf1_p1	rps15-ycf1	1	CCGCAGACCTCTCTGAGATA	GTCTCCTGGCTAGCCTGATA	20	20	55.0	55.0	58.0	58.0	8.0	35.0	0.028240	121226	121626	1216-1216	0.500000	59.8
rps15-ycf1_p2	rps15-ycf1	2	CCGCAGACCTCTCTGAGATA	ATAGTCTCCTGGCTAGCCTG	20	20	55.0	55.0	58.0	58.0	8.0	33.1	0.033977	121226	121626	1219-1219	0.500000	59.7
rps15-ycf1_p3	rps15-ycf1	3	AACACCATGCATTGATGGGT	GTCTCCTGGCTAGCCTGATA	20	20	45.0	55.0	58.1	58.0	8.9	35.0	0.061128	121226	121626	1493-1493	0.500000	59.5
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	ATAGTCTCCTGGCTAGCCTG	20	20	45.0	55.0	58.1	58.0	8.9	33.1	0.066865	121226	121626	1496-1496	0.500000	59.5
rps15-ycf1_p5	rps15-ycf1	5	CCGCAGACCTCTCTGAGATA	AATTATGGAACCTGCCCGTC	20	20	55.0	50.0	58.0	57.9	8.0	0.0	0.078517	121226	121626	1092-1092	0.500000	59.4