primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnQ-UUG_p1	matK-trnQ-UUG	1	TTGAAATTCCGACGTTGACG	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	57.1	58.7	3.7	0.0	1.613600	1547	2150	683-2027	1.000000	71.9
matK-trnQ-UUG_p2	matK-trnQ-UUG	2	TTGAAATTCCGACGTTGACG	GTAAGGCAACGGGTTTTGGT	20	20	45.0	50.0	57.1	59.0	3.7	0.0	1.899751	1547	2150	684-2028	1.000000	69.6
matK-trnQ-UUG_p3	matK-trnQ-UUG	3	TTTGAAATTCCGACGTTGACG	TAAGGCAACGGGTTTTGGTC	21	20	42.9	50.0	57.8	58.7	3.7	0.0	1.921276	1547	2150	684-2028	1.000000	69.5
matK-trnQ-UUG_p4	matK-trnQ-UUG	4	TTGAAATTCCGACGTTGACG	AAGGTTCGAATCCTTCCGC	20	19	45.0	52.6	57.1	57.8	3.7	19.8	2.088697	1547	2150	653-1997	1.000000	68.2
matK-trnQ-UUG_p5	matK-trnQ-UUG	5	TTGAAATTCCGACGTTGACG	TATAAAGAGATGGGGCGTGG	20	20	45.0	50.0	57.1	56.8	3.7	0.0	2.155697	1547	2150	711-2055	1.000000	67.5
trnQ-UUG-rps2_p1	trnQ-UUG-rps2	1	GACCAAAACCCGTTGCCTTA	AGATTTTTCGATTCCGGCCA	20	20	50.0	45.0	58.7	57.9	0.0	2.2	0.802276	2223	2786	659-711	1.000000	80.0
trnQ-UUG-rps2_p2	trnQ-UUG-rps2	2	ATGAAGACAAAACTGGGGCG	AGATTTTTCGATTCCGGCCA	20	20	50.0	45.0	58.8	57.9	0.0	2.2	0.875216	2223	2786	705-757	1.000000	79.3
trnQ-UUG-rps2_p3	trnQ-UUG-rps2	3	GACCAAAACCCGTTGCCTTA	GCAATTTGTGAGGGTCGTTC	20	20	50.0	50.0	58.7	57.7	0.0	0.0	1.011302	2223	2786	589-641	1.000000	78.6
trnQ-UUG-rps2_p4	trnQ-UUG-rps2	4	GACCAAAACCCGTTGCCTTA	TTGTGAGGGTCGTTCTAGCT	20	20	50.0	50.0	58.7	58.4	0.0	0.0	1.050451	2223	2786	584-636	1.000000	78.4
trnQ-UUG-rps2_p5	trnQ-UUG-rps2	5	ATGAAGACAAAACTGGGGCG	GCAATTTGTGAGGGTCGTTC	20	20	50.0	50.0	58.8	57.7	0.0	0.0	1.084242	2223	2786	635-687	1.000000	77.9
trnD-GUC-trnfM-CAU_p1	trnD-GUC-trnfM-CAU	1	CGCTCTGACCGATTGAACTA	AAATCCCGGTTTTTCAACGC	20	20	50.0	45.0	57.4	57.9	0.0	0.0	0.704741	4731	6110	1498-2354	1.000000	80.8
trnD-GUC-trnfM-CAU_p2	trnD-GUC-trnfM-CAU	2	CGCTCTGACCGATTGAACTA	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.786802	4731	6110	1462-2318	1.000000	80.3
trnD-GUC-trnfM-CAU_p3	trnD-GUC-trnfM-CAU	3	CGCTCTGACCGATTGAACTA	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	57.4	57.8	0.0	0.0	0.786802	4731	6110	1461-2317	1.000000	80.3
trnD-GUC-trnfM-CAU_p4	trnD-GUC-trnfM-CAU	4	CGCTCTGACCGATTGAACTA	TTTTCAACGCGGAGTAGAGC	20	20	50.0	50.0	57.4	58.6	0.0	12.5	1.136321	4731	6110	1488-2344	1.000000	77.4
trnD-GUC-trnfM-CAU_p5	trnD-GUC-trnfM-CAU	5	CGCTCTGACCGATTGAACTA	GGTTTTTCAACGCGGAGTAG	20	20	50.0	50.0	57.4	57.4	0.0	12.5	1.151071	4731	6110	1491-2347	1.000000	77.3
accD-rps18_p1	accD-rps18	1	AAATACAGGATCGCGGAACC	ATGCGCAGTTACGAAGGATT	20	20	50.0	45.0	58.1	58.0	6.1	18.3	0.074710	13079	13198	1243-1508	1.000000	91.1
accD-rps18_p2	accD-rps18	2	AAATACAGGATCGCGGAACC	CCCCGACCCCCTATAAGTAG	20	20	50.0	60.0	58.1	58.1	6.1	0.0	0.116125	13079	13198	891-1174	1.000000	91.1
accD-rps18_p3	accD-rps18	3	AAATACAGGATCGCGGAACC	CCCGACCCCCTATAAGTAGG	20	20	50.0	60.0	58.1	58.1	6.1	0.0	0.116125	13079	13198	890-1173	1.000000	91.1
accD-rps18_p4	accD-rps18	4	AAATACAGGATCGCGGAACC	CGATGTCGTAGGTTCGAGTC	20	20	50.0	55.0	58.1	57.9	6.1	0.0	0.138873	13079	13198	1044-1315	1.000000	90.9
accD-rps18_p5	accD-rps18	5	AAATACAGGATCGCGGAACC	CAAAAAGCCCCTTGAGGTCT	20	20	50.0	50.0	58.1	58.0	6.1	0.0	0.051611	13079	13198	1441-1441	0.500000	59.6
rpl20-rps12_p1	rpl20-rps12	1	GCCCCTATAAAGGTTGAGGC	TGTCACTAAATCTCCTGCGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.027485	15688	16679	1136-1187	1.000000	84.3
rpl20-rps12_p2	rpl20-rps12	2	GCCCCTATAAAGGTTGAGGC	ACTAAATCTCCTGCGCTTCG	20	20	55.0	50.0	58.0	58.1	0.0	9.7	0.089445	15688	16679	1132-1183	1.000000	83.8
rpl20-rps12_p3	rpl20-rps12	3	GCCCCTATAAAGGTTGAGGC	CTAAATCTCCTGCGCTTCGA	20	20	55.0	50.0	58.0	57.8	0.0	9.7	0.227178	15688	16679	1131-1182	1.000000	82.7
rpl20-rps12_p4	rpl20-rps12	4	GCCCCTATAAAGGTTGAGGC	TAAATCTCCTGCGCTTCGAG	20	20	55.0	50.0	58.0	57.8	0.0	9.7	0.227178	15688	16679	1130-1181	1.000000	82.7
rpl20-rps12_p5	rpl20-rps12	5	TTCTTTTTCGACGTCTCCGG	TGTCACTAAATCTCCTGCGC	20	20	50.0	50.0	58.2	58.0	14.7	0.0	0.235506	15688	16679	1105-1156	1.000000	82.7
rpl14-rpl16_p1	rpl14-rpl16	1	CAACTCTTTAGCTCCGCTGT	ATACGCTCTTCAGGCACTTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.101790	22314	22682	737-754	1.000000	87.8
rpl14-rpl16_p2	rpl14-rpl16	2	GGGTTTCCTGCTTGATCGAT	ATACGCTCTTCAGGCACTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059859	22314	22682	958-975	1.000000	86.2
rpl14-rpl16_p3	rpl14-rpl16	3	GGGTTTCCTGCTTGATCGAT	CCTGCTTGGATCACTGCTAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.093295	22314	22682	936-953	1.000000	86.1
rpl14-rpl16_p4	rpl14-rpl16	4	ACTTCGGGCGCTAATGAAAT	ATACGCTCTTCAGGCACTTG	20	20	45.0	50.0	58.0	58.0	7.6	0.0	0.054123	22314	22682	1042-1059	1.000000	85.7
rpl14-rpl16_p5	rpl14-rpl16	5	ACTTCGGGCGCTAATGAAAT	CCTGCTTGGATCACTGCTAG	20	20	45.0	55.0	58.0	58.0	7.6	0.0	0.087559	22314	22682	1020-1037	1.000000	85.6
rps19-rpl2_p1	rps19-rpl2	1	TGCCCGAGACCAAGTTACTA	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.1	58.0	0.0	22.4	0.096688	26149	26761	801-947	1.000000	88.6
rps19-rpl2_p2	rps19-rpl2	2	TGCCCGAGACCAAGTTACTA	GCTTGGAAGAAGAAGCAGGA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.263640	26149	26761	791-937	1.000000	87.4
rps19-rpl2_p3	rps19-rpl2	3	GATGCCCGAGACCAAGTTAC	GTTATCCTGCGCTTGGAAGA	20	20	55.0	50.0	58.3	58.0	0.0	22.4	0.371024	26149	26761	803-949	1.000000	86.4
rps19-rpl2_p4	rps19-rpl2	4	TGCCCGAGACCAAGTTACTA	TGATTCTTCGTCGCCGTAAA	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.423412	26149	26761	747-893	1.000000	86.4
rps19-rpl2_p5	rps19-rpl2	5	TGCCCGAGACCAAGTTACTA	TATCCTGCGCTTGGAAGAAG	20	20	50.0	50.0	58.1	57.7	0.0	22.4	0.388438	26149	26761	799-945	1.000000	86.3
rpl2-rpl23_p1	rpl2-rpl23	1	ATTCCATTGCGTGTACTCGG	ATCCGTTATGGGACCAATGC	20	20	50.0	50.0	58.3	58.0	18.0	0.0	0.366756	28325	28828	521-639	1.000000	85.3
rpl2-rpl23_p2	rpl2-rpl23	2	ACGGTGCTGTCCAAAAATCA	ATCCGTTATGGGACCAATGC	20	20	45.0	50.0	58.3	58.0	0.0	0.0	0.332389	28325	28828	588-706	1.000000	85.2
rpl2-rpl23_p3	rpl2-rpl23	3	ACGATTCCATTGCGTGTACT	ATCCGTTATGGGACCAATGC	20	20	45.0	50.0	57.6	58.0	0.0	0.0	0.398827	28325	28828	524-642	1.000000	85.1
rpl2-rpl23_p4	rpl2-rpl23	4	CGTGTACTCGGGGTAGAAGT	ATCCGTTATGGGACCAATGC	20	20	55.0	50.0	58.5	58.0	9.4	0.0	0.561397	28325	28828	512-630	1.000000	83.9
rpl2-rpl23_p5	rpl2-rpl23	5	CAACGGTGCTGTCCAAAAAT	ATCCGTTATGGGACCAATGC	20	20	45.0	50.0	57.5	58.0	0.0	0.0	0.526083	28325	28828	590-708	1.000000	83.6
rps12_p1	rps12	1	GGCGCGAAAGAGGTTAAAGA	ACGAGGAGCCATATGAGATGA	20	21	50.0	47.6	58.6	58.1	12.1	3.7	1.682689	39141	40855	1857-1889	1.000000	73.0
rps12_p2	rps12	2	GGCGCGAAAGAGGTTAAAGA	TGAACGAGGAGCCATATGAG	20	20	50.0	50.0	58.6	56.8	12.1	3.7	1.750903	39141	40855	1860-1892	1.000000	72.4
rps12_p3	rps12	3	TTTTCTTCTGCAGCACCTCG	ACGAGGAGCCATATGAGATGA	20	21	50.0	47.6	58.8	58.1	9.5	3.7	1.888838	39141	40855	1887-1919	1.000000	71.3
rps12_p4	rps12	4	GGCGCGAAAGAGGTTAAAGA	TGAACGAGGAGCCATATGAGA	20	21	50.0	47.6	58.6	58.3	12.1	3.7	1.898106	39141	40855	1860-1892	1.000000	71.2
rps12_p5	rps12	5	TTTTCTTCTGCAGCACCTCG	TGAACGAGGAGCCATATGAG	20	20	50.0	50.0	58.8	56.8	9.5	3.7	1.957052	39141	40855	1890-1922	1.000000	70.7
rrn16-rrn23_p1	rrn16-rrn23	1	CAAGGTAGCCGTACTGGAAG	ATTCGGGAATCTCCGGATCT	20	20	55.0	50.0	57.7	58.0	7.8	3.6	0.312158	44581	46342	1918-2081	1.000000	87.3
rrn16-rrn23_p2	rrn16-rrn23	2	CAAGGTAGCCGTACTGGAAG	GTCTCTGGGTGCCTAGGTAT	20	20	55.0	55.0	57.7	57.9	7.8	11.3	0.384853	44581	46342	1846-2009	1.000000	87.0
rrn16-rrn23_p3	rrn16-rrn23	3	AAGTCGTAACAAGGTAGCCG	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	57.6	58.0	0.0	3.6	0.375844	44581	46342	1927-2090	1.000000	86.8
rrn16-rrn23_p4	rrn16-rrn23	4	AACAAGGTAGCCGTACTGGA	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.4	58.0	7.8	3.6	0.380756	44581	46342	1920-2083	1.000000	86.8
rrn16-rrn23_p5	rrn16-rrn23	5	ACAAGGTAGCCGTACTGGAA	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.4	58.0	7.8	3.6	0.380756	44581	46342	1919-2082	1.000000	86.8
rrn4.5-rrn5_p1	rrn4.5-rrn5	1	TGCAGTTGAGGCATCCTAAT	CCTCTATGCCTAGGACACCA	20	20	45.0	55.0	56.9	57.9	0.0	14.8	1.196425	49332	49514	246-265	1.000000	76.4
rrn4.5-rrn5_p2	rrn4.5-rrn5	2	TGCAGTTGAGGCATCCTAAT	AAGTTCGGGATGGATTGGTG	20	20	45.0	50.0	56.9	57.9	0.0	0.0	1.237092	49332	49514	271-290	1.000000	75.5
rrn4.5-rrn5_p3	rrn4.5-rrn5	3	TGCAGTTGAGGCATCCTAAT	ACCAAGTTCGGGATGGATTG	20	20	45.0	50.0	56.9	57.9	0.0	0.0	1.237092	49332	49514	274-293	1.000000	75.5
rrn4.5-rrn5_p4	rrn4.5-rrn5	4	TGCAGTTGAGGCATCCTAAT	CAAGTTCGGGATGGATTGGT	20	20	45.0	50.0	56.9	57.9	0.0	0.0	1.237092	49332	49514	272-291	1.000000	75.5
rrn4.5-rrn5_p5	rrn4.5-rrn5	5	TGCAGTTGAGGCATCCTAAT	CACCAAGTTCGGGATGGATT	20	20	45.0	50.0	56.9	57.9	0.0	0.0	1.237092	49332	49514	275-294	1.000000	75.4
rrn5-trnN-GUU_p1	rrn5-trnN-GUU	1	AGGAGGGATCCTGTGGAAAA	AGGTCGTAGGTTCGAATCCT	20	20	50.0	50.0	57.9	57.9	12.6	18.9	0.200595	49636	51111	1528-1544	1.000000	88.4
rrn5-trnN-GUU_p2	rrn5-trnN-GUU	2	TGGTGTCCTAGGCATAGAGG	AGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.9	57.9	7.0	18.9	0.228943	49636	51111	1606-1622	1.000000	87.9
rrn5-trnN-GUU_p3	rrn5-trnN-GUU	3	AATCCATCCCGAACTTGGTG	AGGTCGTAGGTTCGAATCCT	20	20	50.0	50.0	57.9	57.9	0.0	18.9	0.269609	49636	51111	1577-1593	1.000000	87.7
rrn5-trnN-GUU_p4	rrn5-trnN-GUU	4	ACCAATCCATCCCGAACTTG	AGGTCGTAGGTTCGAATCCT	20	20	50.0	50.0	57.9	57.9	0.0	18.9	0.269609	49636	51111	1580-1596	1.000000	87.7
rrn5-trnN-GUU_p5	rrn5-trnN-GUU	5	CAATCCATCCCGAACTTGGT	AGGTCGTAGGTTCGAATCCT	20	20	50.0	50.0	57.9	57.9	0.0	18.9	0.269609	49636	51111	1578-1594	1.000000	87.7
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	CTCTACCGCTGAGCTACTGA	TAGCGCAACGACAGAATTGA	20	20	55.0	45.0	58.3	57.9	1.8	11.5	0.403512	51184	52062	965-1007	1.000000	88.8
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	TCTACCGCTGAGCTACTGAG	TAGCGCAACGACAGAATTGA	20	20	55.0	45.0	58.3	57.9	1.8	11.5	0.403512	51184	52062	964-1006	1.000000	88.8
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	CTCTACCGCTGAGCTACTGA	AATATAGCCCTAGCGCAACG	20	20	55.0	50.0	58.3	57.9	1.8	11.5	0.410682	51184	52062	975-1017	1.000000	88.7
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	TCTACCGCTGAGCTACTGAG	AATATAGCCCTAGCGCAACG	20	20	55.0	50.0	58.3	57.9	1.8	11.5	0.410682	51184	52062	974-1016	1.000000	88.7
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	TCTACCGCTGAGCTACTGAG	GCGCAACGACAGAATTGATT	20	20	55.0	45.0	58.3	57.8	1.8	0.3	0.577706	51184	52062	962-1004	1.000000	87.4
rps12_p1	rps12	1	ACGAGGAGCCATATGAGATGA	GGCGCGAAAGAGGTTAAAGA	21	20	47.6	50.0	58.1	58.6	3.7	12.1	1.682689	57841	59555	1857-1889	1.000000	73.0
rps12_p2	rps12	2	TGAACGAGGAGCCATATGAG	GGCGCGAAAGAGGTTAAAGA	20	20	50.0	50.0	56.8	58.6	3.7	12.1	1.750903	57841	59555	1860-1892	1.000000	72.4
rps12_p3	rps12	3	ACGAGGAGCCATATGAGATGA	TTTTCTTCTGCAGCACCTCG	21	20	47.6	50.0	58.1	58.8	3.7	9.5	1.888838	57841	59555	1887-1919	1.000000	71.3
rps12_p4	rps12	4	TGAACGAGGAGCCATATGAGA	GGCGCGAAAGAGGTTAAAGA	21	20	47.6	50.0	58.3	58.6	3.7	12.1	1.898106	57841	59555	1860-1892	1.000000	71.2
rps12_p5	rps12	5	TGAACGAGGAGCCATATGAG	TTTTCTTCTGCAGCACCTCG	20	20	50.0	50.0	56.8	58.8	3.7	9.5	1.957052	57841	59555	1890-1922	1.000000	70.7