primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	75	363	340-364	1.000000	87.8
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	75	363	354-378	1.000000	87.6
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	75	363	353-377	1.000000	87.6
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	75	363	355-379	1.000000	87.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.301771	75	363	392-416	1.000000	86.3
trnK-UUU_p1	trnK-UUU	1	ACTCCCAAGCACACGAATTT	TCAACGGTTTGATTAGCTCGT	20	21	45.0	42.9	58.0	58.0	0.0	0.0	1.053464	1654	4239	2916-2928	1.000000	74.6
trnK-UUU_p2	trnK-UUU	2	TTCGTGTCAACCAATCCCAA	TCAACGGTTTGATTAGCTCGT	20	21	45.0	42.9	57.6	58.0	0.0	0.0	1.384778	1654	4239	2836-2848	1.000000	72.0
trnK-UUU_p3	trnK-UUU	3	GTTCGTGTCAACCAATCCCA	TCAACGGTTTGATTAGCTCGT	20	21	50.0	42.9	58.4	58.0	0.0	0.0	1.434697	1654	4239	2837-2849	1.000000	71.6
trnK-UUU_p4	trnK-UUU	4	TGTTCGTGTCAACCAATCCC	TCAACGGTTTGATTAGCTCGT	20	21	50.0	42.9	58.4	58.0	0.0	0.0	1.434697	1654	4239	2838-2850	1.000000	71.6
trnK-UUU_p5	trnK-UUU	5	GACTCCCAAGCACACGAATT	TCAACGGTTTGATTAGCTCGT	20	21	50.0	42.9	58.5	58.0	0.0	0.0	1.510963	1654	4239	2917-2929	1.000000	70.9
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	ACAACGGGTCCTATCCTTCA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.129117	5843	6733	983-1070	1.000000	87.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5843	6733	964-1051	1.000000	86.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGTCCTATCCTTCAAGTCGC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.8	58.1	0.0	16.9	0.302703	5843	6733	977-1064	1.000000	85.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ACGGGTCCTATCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.325410	5843	6733	980-1067	1.000000	85.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CTATCCTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.7	58.1	0.0	16.9	0.355683	5843	6733	973-1060	1.000000	85.4
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	ATGATCCAGGGCGTAATCCT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.269928	7845	7993	245-257	1.000000	84.4
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	ATGATCCAGGGCGTAATCCT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.3	58.0	0.0	0.0	0.271316	7845	7993	246-258	1.000000	84.4
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	ATGATCCAGGGCGTAATCCT	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.374793	7845	7993	264-276	1.000000	83.2
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	AATGATCCAGGGCGTAATCC	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.573205	7845	7993	246-258	1.000000	81.9
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	AATGATCCAGGGCGTAATCC	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.574593	7845	7993	247-259	1.000000	81.9
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.241837	8082	8771	730-795	1.000000	77.2
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.243225	8082	8771	729-794	1.000000	77.2
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.248863	8082	8771	730-795	1.000000	77.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.250251	8082	8771	729-794	1.000000	77.2
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.2	58.2	0.0	1.2	1.388603	8082	8771	784-849	1.000000	75.9
atpI-rps2_p1	atpI-rps2	1	ATGTTGGCCTACTTCCACAC	ATTTCGATTCCAGCGAACGA	20	20	50.0	45.0	57.8	58.0	18.3	0.0	0.204955	15045	15269	406-408	1.000000	83.1
atpI-rps2_p2	atpI-rps2	2	ATGTTGGCCTACTTCCACAC	TTTCGATTCCAGCGAACGAT	20	20	50.0	45.0	57.8	58.0	18.3	0.0	0.204955	15045	15269	405-407	1.000000	83.1
atpI-rps2_p3	atpI-rps2	3	CGTGTACTTGAAAACCCCCT	ATTTCGATTCCAGCGAACGA	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.270171	15045	15269	440-442	1.000000	82.2
atpI-rps2_p4	atpI-rps2	4	CGTGTACTTGAAAACCCCCT	TTTCGATTCCAGCGAACGAT	20	20	50.0	45.0	57.7	58.0	0.0	0.0	0.270171	15045	15269	439-441	1.000000	82.2
atpI-rps2_p5	atpI-rps2	5	ACTTGGCCGTGTACTTGAAA	TTTCGATTCCAGCGAACGAT	20	20	45.0	45.0	57.7	58.0	0.0	0.0	0.339898	15045	15269	446-448	1.000000	81.6
trnE-UUC-trnT_p1	trnE-UUC-trnT	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	30159	30752	701-735	1.000000	84.9
trnE-UUC-trnT_p2	trnE-UUC-trnT	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	30159	30752	680-714	1.000000	84.5
trnE-UUC-trnT_p3	trnE-UUC-trnT	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	30159	30752	677-711	1.000000	84.3
trnE-UUC-trnT_p4	trnE-UUC-trnT	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30159	30752	663-697	1.000000	84.2
trnE-UUC-trnT_p5	trnE-UUC-trnT	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	30159	30752	698-732	1.000000	83.7
ycf3-trnS_p1	ycf3-trnS	1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.961472	43842	44615	946-1008	1.000000	76.7
ycf3-trnS_p2	ycf3-trnS	2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.963464	43842	44615	945-1007	1.000000	76.7
ycf3-trnS_p3	ycf3-trnS	3	TGCCTCCTTTTCTCCTGAAG	AAGTGAAGGTGCGGAAAGAG	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.982874	43842	44615	977-1039	1.000000	76.4
ycf3-trnS_p4	ycf3-trnS	4	GCCTCCTTTTCTCCTGAAGT	AAGTGAAGGTGCGGAAAGAG	20	20	50.0	50.0	57.1	58.1	0.0	0.0	0.984866	43842	44615	976-1038	1.000000	76.4
ycf3-trnS_p5	ycf3-trnS	5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	57.1	57.8	0.0	5.6	1.059773	43842	44615	917-979	1.000000	76.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	20	20	55.0	55.0	58.0	58.0	10.3	0.0	0.015623	47698	48381	825-836	1.000000	84.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.065013	47698	48381	789-800	1.000000	83.9
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GAGGGTGGGATGCTACATTG	GTTGGATAGGATGGCCCTTG	20	20	55.0	55.0	58.0	58.0	2.6	0.0	0.040246	47698	48381	854-865	1.000000	83.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GGGATAGCTCAGCTGGTAGA	TGCCTGACAGTTGGATAGGA	20	20	55.0	50.0	58.0	58.0	10.3	0.9	0.051296	47698	48381	834-845	1.000000	83.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GAGGGTGGGATGCTACATTG	TGCCTGACAGTTGGATAGGA	20	20	55.0	50.0	58.0	58.0	2.6	0.9	0.075919	47698	48381	863-874	1.000000	83.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCATATTCGTAAAGCAAAAACA	AGAAGGTCTACGGTTCGAGT	22	20	27.3	50.0	52.7	57.8	0.0	0.0	7.479993	50069	51001	968-989	1.000000	46.9
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCGTAAAGCAAAAACA	GAAGGTCTACGGTTCGAGTC	22	20	27.3	55.0	52.7	57.5	0.0	0.0	7.823765	50069	51001	967-988	1.000000	46.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	AGACTTGATATTATTAGAAATGCCCA	AGAAGGTCTACGGTTCGAGT	26	20	30.8	50.0	56.5	57.8	0.0	0.0	7.658154	50069	51001	1001-1022	1.000000	46.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCATATTCGTAAAGCAAAAACA	AAGGCATGTTGGGTCTTTGA	22	20	27.3	45.0	52.7	57.6	0.0	0.0	7.662774	50069	51001	1035-1056	1.000000	46.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCATATTCGTAAAGCAAAAACA	AGGCATGTTGGGTCTTTGAA	22	20	27.3	45.0	52.7	57.6	0.0	0.0	7.662774	50069	51001	1034-1055	1.000000	46.7
rps8-rpl14_p1	rps8-rpl14	1	AGATGCTATGCGAACAACTCT	CTCGCATTTTTGGGGCAATC	21	20	42.9	50.0	57.5	58.4	0.0	0.0	1.889726	79404	79584	343-355	1.000000	67.9
rps8-rpl14_p2	rps8-rpl14	2	AGATGCTATGCGAACAACTCT	AGGAACTCGCATTTTTGGGG	21	20	42.9	50.0	57.5	58.7	0.0	0.0	2.276794	79404	79584	348-360	1.000000	64.7
rps8-rpl14_p3	rps8-rpl14	3	AGATGCTATGCGAACAACTC	CTCGCATTTTTGGGGCAATC	20	20	45.0	50.0	55.9	58.4	0.0	0.0	2.409773	79404	79584	343-355	1.000000	63.7
rps8-rpl14_p4	rps8-rpl14	4	GATGCTATGCGAACAACTCT	CTCGCATTTTTGGGGCAATC	20	20	45.0	50.0	55.9	58.4	0.0	0.0	2.409773	79404	79584	342-354	1.000000	63.7
rps8-rpl14_p5	rps8-rpl14	5	AGATGCTATGCGAACAACTCT	CCCCGCGAATTGAGACAATT	21	20	42.9	50.0	57.5	58.9	0.0	8.2	2.438821	79404	79584	323-335	1.000000	63.6
ndhF-rpl32_p1	ndhF-rpl32	1	GCCCACATACGACGAAGATT	TGCATGAATTGATGGCGAGA	20	20	50.0	45.0	58.1	58.0	0.0	7.0	0.105319	110707	111146	1117-1154	1.000000	86.3
ndhF-rpl32_p2	ndhF-rpl32	2	GCCCACATACGACGAAGATT	TGAATTGATGGCGAGAGGTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.092487	110707	111146	1113-1150	0.750000	72.4
ndhF-rpl32_p3	ndhF-rpl32	3	GCCCACATACGACGAAGATT	GAATTGATGGCGAGAGGTGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.093906	110707	111146	1112-1149	0.750000	72.4
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGCCCACATACGACGAA	TGAATTGATGGCGAGAGGTG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.201271	110707	111146	1117-1154	0.750000	71.5
ndhF-rpl32_p5	ndhF-rpl32	5	GCCCACATACGACGAAGATT	ACTCCCCCTCTGGAAAGAAA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.197925	110707	111146	980-999	0.500000	63.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCCCACATACGACGAAGATT	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071898	111318	112088	1408-1567	1.000000	88.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCCCACATACGACGAAGATT	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073268	111318	112088	1409-1568	1.000000	88.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCCCACATACGACGAAGATT	TCATGCCCTTTTCGGATGAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.106753	111318	112088	1691-1844	1.000000	87.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCCCACATACGACGAAGATT	ATGCCCTTTTCGGATGAGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.108190	111318	112088	1689-1842	1.000000	87.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCCCACATACGACGAAGATT	GCCCTTTTCGGATGAGTCAT	20	20	50.0	50.0	58.1	58.0	0.0	5.0	0.108190	111318	112088	1687-1840	1.000000	87.7
ycf1_p1	ycf1	1	CAGGCCTCAATATCGGCTTT	AGTTCGCCAGCGTTGTATAG	20	20	50.0	50.0	58.0	58.1	0.0	12.3	0.104558	122898	123017	402-414	1.000000	87.1
ycf1_p2	ycf1	2	AAATTCGATATGGGGCGCTT	AGTTCGCCAGCGTTGTATAG	20	20	45.0	50.0	58.0	58.1	7.6	12.3	0.096173	122898	123017	597-597	0.750000	71.0
ycf1_p3	ycf1	3	AATTCGATATGGGGCGCTTT	AGTTCGCCAGCGTTGTATAG	20	20	45.0	50.0	58.0	58.1	7.6	12.3	0.096173	122898	123017	596-596	0.750000	71.0
ycf1_p4	ycf1	4	AAATTCGATATGGGGCGCTT	GAGTTCGCCAGCGTTGTATA	20	20	45.0	50.0	58.0	58.1	7.6	12.3	0.101471	122898	123017	598-598	0.750000	70.9
ycf1_p5	ycf1	5	AATTCGATATGGGGCGCTTT	GAGTTCGCCAGCGTTGTATA	20	20	45.0	50.0	58.0	58.1	7.6	12.3	0.101471	122898	123017	597-597	0.750000	70.9