primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
petB_p1	petB	1	AGGGATCCAAACTCAGACCA	CCGTTGTTACTCGGAACAAG	20	20	50.0	50.0	58.0	56.8	15.2	7.7	1.267655	23943	25384	1599-1599	1.000000	70.9
petB_p2	petB	2	AGGGATCCAAACTCAGACCA	CCGTTGTTACTCGGAACAAGA	20	21	50.0	47.6	58.0	58.3	15.2	7.7	1.298524	23943	25384	1599-1599	1.000000	70.6
petB_p3	petB	3	AGGGATCCAAACTCAGACCA	ACCGTTGTTACTCGGAACAAG	20	21	50.0	47.6	58.0	58.5	15.2	20.9	1.567972	23943	25384	1600-1600	1.000000	68.5
petB_p4	petB	4	GAGGGATCCAAACTCAGACC	CCGTTGTTACTCGGAACAAG	20	20	55.0	50.0	57.3	56.8	15.2	7.7	1.923584	23943	25384	1600-1600	1.000000	65.6
petB_p5	petB	5	GAGGGATCCAAACTCAGACC	CCGTTGTTACTCGGAACAAGA	20	21	55.0	47.6	57.3	58.3	15.2	7.7	1.954453	23943	25384	1600-1600	1.000000	65.4
ndhK_p1	ndhK	1	TCGCTCCAGTTCTTGTTGTT	TCCGAACCGAAATGGAATGT	20	20	45.0	45.0	57.7	57.5	0.0	0.0	0.808597	35801	36600	950-957	1.000000	75.2
ndhK_p2	ndhK	2	TCGCTCCAGTTCTTGTTGTT	CTATTCATCCCGTCCGAACC	20	20	45.0	55.0	57.7	57.8	0.0	0.0	0.475980	35801	36600	962-969	0.416667	53.9
ndhK_p3	ndhK	3	TTCGCTCCAGTTCTTGTTGT	CTATTCATCCCGTCCGAACC	20	20	45.0	55.0	57.7	57.8	0.0	0.0	0.475980	35801	36600	963-970	0.416667	53.9
ndhK_p4	ndhK	4	TGTTCGCTCCAGTTCTTGTT	CTATTCATCCCGTCCGAACC	20	20	45.0	55.0	57.7	57.8	0.0	0.0	0.475980	35801	36600	965-972	0.416667	53.8
ndhK_p5	ndhK	5	ATGTTCGCTCCAGTTCTTGT	CTATTCATCCCGTCCGAACC	20	20	45.0	55.0	57.4	57.8	0.0	0.0	0.701227	35801	36600	966-973	0.416667	52.0
ycf1_p1	ycf1	1	AGGGCAATGAACAGGTCCTA	GTCTCATCAATCCCCTGCTG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.366622	69722	69841	1172-1241	1.000000	84.8
ycf1_p2	ycf1	2	AGGGCAATGAACAGGTCCTA	GGTACCCTTCCAATCCTCCT	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.436475	69722	69841	861-942	1.000000	84.7
ycf1_p3	ycf1	3	AGGGCAATGAACAGGTCCTA	ATCCGGATCCTCTCTTCTCG	20	20	50.0	55.0	58.3	58.1	0.0	12.4	0.426887	69722	69841	1231-1300	1.000000	84.2
ycf1_p4	ycf1	4	AGGGCAATGAACAGGTCCTA	CCGGATCCTCTCTTCTCGAT	20	20	50.0	55.0	58.3	58.1	0.0	10.8	0.426887	69722	69841	1229-1298	1.000000	84.2
ycf1_p5	ycf1	5	AGGGCAATGAACAGGTCCTA	TGATTCCCAAAAGTTCCGGG	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.413302	69722	69841	1273-1342	1.000000	84.1
trnP-GGG-rpl32_p1	trnP-GGG-rpl32	1	TGGTTATAACGGAGCATGGC	AAGTCTATCTCCACCGGTCA	20	20	50.0	50.0	58.0	57.5	9.5	2.6	0.565094	76600	77706	767-1298	1.000000	80.1
trnP-GGG-rpl32_p2	trnP-GGG-rpl32	2	TGGTTATAACGGAGCATGGC	AGTCTATCTCCACCGGTCAT	20	20	50.0	50.0	58.0	57.2	9.5	2.6	0.796101	76600	77706	766-1297	1.000000	78.3
trnP-GGG-rpl32_p3	trnP-GGG-rpl32	3	TGGTTATAACGGAGCATGGC	TCTCCACCGGTCATTCAAAA	20	20	50.0	45.0	58.0	57.1	9.5	2.6	0.979201	76600	77706	760-1291	1.000000	76.8
trnP-GGG-rpl32_p4	trnP-GGG-rpl32	4	TGGTTATAACGGAGCATGGC	TCTATCTCCACCGGTCATTCA	20	21	50.0	47.6	58.0	58.0	9.5	2.6	1.064914	76600	77706	764-1295	1.000000	76.1
trnP-GGG-rpl32_p5	trnP-GGG-rpl32	5	TGGTTATAACGGAGCATGGC	AGTCTATCTCCACCGGTCATT	20	21	50.0	47.6	58.0	58.0	9.5	2.6	1.071866	76600	77706	766-1297	1.000000	76.1
ycf2_p1	ycf2	1	TTTCCATGATATATTCTCATCCA	AGCTCTCTTCACTCCAGAGG	23	20	30.4	55.0	52.8	58.1	0.0	0.0	8.390429	78147	78733	729-734	1.000000	42.2
ycf2_p2	ycf2	2	TTTCCATGATATATTCTCATCCA	CTCTCTTCACTCCAGAGGCT	23	20	30.4	55.0	52.8	58.1	0.0	0.0	8.390429	78147	78733	727-732	1.000000	42.2
ycf2_p3	ycf2	3	TTTCCATGATATATTCTCATCCA	TCTCTTCACTCCAGAGGCTC	23	20	30.4	55.0	52.8	58.2	0.0	6.3	8.395726	78147	78733	726-731	1.000000	42.2
ycf2_p4	ycf2	4	TCCATGATATATTCTCATCCATT	AGCTCTCTTCACTCCAGAGG	23	20	30.4	55.0	52.5	58.1	0.0	0.0	8.610725	78147	78733	727-732	1.000000	42.2
ycf2_p5	ycf2	5	TCCATGATATATTCTCATCCATT	CTCTCTTCACTCCAGAGGCT	23	20	30.4	55.0	52.5	58.1	0.0	0.0	8.610725	78147	78733	725-730	1.000000	42.2
trnV-GAC-rps12_p1	trnV-GAC-rps12	1	GATGACTTCCACCACGTCAA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.421509	79129	80718	1673-1688	1.000000	78.5
trnV-GAC-rps12_p2	trnV-GAC-rps12	2	AACTGATGACTTCCACCACG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	79129	80718	1677-1692	1.000000	78.5
trnV-GAC-rps12_p3	trnV-GAC-rps12	3	ATGACTTCCACCACGTCAAG	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	0.0	16.5	0.427523	79129	80718	1672-1687	1.000000	78.5
trnV-GAC-rps12_p4	trnV-GAC-rps12	4	CTGATGACTTCCACCACGTC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.3	57.7	0.0	16.5	0.542896	79129	80718	1675-1690	1.000000	77.6
trnV-GAC-rps12_p5	trnV-GAC-rps12	5	GGCTCGAACTGATGACTTCC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.4	57.7	0.0	16.5	0.611502	79129	80718	1683-1698	1.000000	77.0
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	GAAGTTATCTTTCCCCGGCC	GTCTCGCGTGTCTACCATTT	20	20	55.0	50.0	58.3	58.0	0.0	5.8	0.331084	82473	84068	1652-1662	1.000000	78.9
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	GGAAGTTATCTTTCCCCGGC	GTCTCGCGTGTCTACCATTT	20	20	55.0	50.0	58.3	58.0	0.0	5.8	0.331084	82473	84068	1653-1663	1.000000	78.9
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	GAAGTTATCTTTCCCCGGCC	CTCGCGTGTCTACCATTTCA	20	20	55.0	50.0	58.3	58.0	0.0	5.8	0.332455	82473	84068	1650-1660	1.000000	78.9
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	GGAAGTTATCTTTCCCCGGC	CTCGCGTGTCTACCATTTCA	20	20	55.0	50.0	58.3	58.0	0.0	5.8	0.332455	82473	84068	1651-1661	1.000000	78.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TATCTTTCCCCGGCCAGAAT	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	58.5	58.0	2.2	5.8	0.489463	82473	84068	1647-1657	1.000000	77.7
trnL-CAA-ycf2_p1	trnL-CAA-ycf2	1	AAATGGTAGACACGCGAGAC	ATCAGCCGGGAGAATCTGTA	20	20	50.0	50.0	58.0	57.9	15.9	2.2	0.095935	84150	84763	698-709	1.000000	81.7
trnL-CAA-ycf2_p2	trnL-CAA-ycf2	2	TGAAATGGTAGACACGCGAG	ATCAGCCGGGAGAATCTGTA	20	20	50.0	50.0	58.0	57.9	15.9	2.2	0.097305	84150	84763	700-711	1.000000	81.7
trnL-CAA-ycf2_p3	trnL-CAA-ycf2	3	AGGATTCACTTTCGGGATGC	ATCAGCCGGGAGAATCTGTA	20	20	50.0	50.0	58.0	57.9	0.0	2.2	0.132227	84150	84763	728-739	1.000000	81.3
trnL-CAA-ycf2_p4	trnL-CAA-ycf2	4	GATTCACTTTCGGGATGCCT	ATCAGCCGGGAGAATCTGTA	20	20	50.0	50.0	58.0	57.9	0.3	2.2	0.132227	84150	84763	726-737	1.000000	81.3
trnL-CAA-ycf2_p5	trnL-CAA-ycf2	5	AAATGGTAGACACGCGAGAC	TCAGCCGGGAGAATCTGTAA	20	20	50.0	50.0	58.0	58.1	15.9	2.2	0.147189	84150	84763	697-708	1.000000	81.3
trnS-GCU-trnT-GGU_p1	trnS-GCU-trnT-GGU	1	ATTAGCAATCCGCCGCTTTA	GAACCGATGACTTACGCCTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.044177	99923	101096	1267-1269	1.000000	81.2
trnS-GCU-trnT-GGU_p2	trnS-GCU-trnT-GGU	2	ATTAGCAATCCGCCGCTTTA	CCATGGCGTTACTCTACCAC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.102274	99923	101096	1246-1248	1.000000	80.7
trnS-GCU-trnT-GGU_p3	trnS-GCU-trnT-GGU	3	ATTAGCAATCCGCCGCTTTA	TGGCGTTACTCTACCACTGA	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.124429	99923	101096	1243-1245	1.000000	80.5
trnS-GCU-trnT-GGU_p4	trnS-GCU-trnT-GGU	4	ATTAGCAATCCGCCGCTTTA	CCGATGACTTACGCCTTACC	20	20	45.0	55.0	58.0	58.2	0.0	0.0	0.189110	99923	101096	1264-1266	1.000000	80.0
trnS-GCU-trnT-GGU_p5	trnS-GCU-trnT-GGU	5	TAGTGACCGAACGGAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	20.1	0.0	0.270584	99923	101096	1326-1328	1.000000	79.3
atpE-atpB_p1	atpE-atpB	1	CTGAATCCCAAACGACTCGA	GCTACCGCGAAGGCTATTAA	20	20	50.0	50.0	57.6	57.9	5.2	8.2	0.508269	104362	104375	90-90	1.000000	77.7
atpE-atpB_p2	atpE-atpB	2	TCTGAATCCCAAACGACTCG	GCTACCGCGAAGGCTATTAA	20	20	50.0	50.0	57.6	57.9	0.0	8.2	0.508269	104362	104375	91-91	1.000000	77.7
atpE-atpB_p3	atpE-atpB	3	CTGAATCCCAAACGACTCGA	ATTGATGAAGCTACCGCGAA	20	20	50.0	45.0	57.6	57.7	5.2	8.2	0.673315	104362	104375	99-99	1.000000	76.2
atpE-atpB_p4	atpE-atpB	4	TCTGAATCCCAAACGACTCG	ATTGATGAAGCTACCGCGAA	20	20	50.0	45.0	57.6	57.7	0.0	8.2	0.673315	104362	104375	100-100	1.000000	76.2
atpE-atpB_p5	atpE-atpB	5	CTGAATCCCAAACGACTCGA	TTGATGAAGCTACCGCGAAG	20	20	50.0	50.0	57.6	58.4	5.2	8.2	0.723360	104362	104375	98-98	1.000000	75.8
atpB-rbcL_p1	atpB-rbcL	1	CGGAAACCCCAAGAACAAGA	CCAGCTTTGAATCCGACACT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.210827	105840	106594	777-861	1.000000	83.0
atpB-rbcL_p2	atpB-rbcL	2	ATTTGAGCGATACGTCCCAC	CCAGCTTTGAATCCGACACT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.250554	105840	106594	814-898	1.000000	82.7
atpB-rbcL_p3	atpB-rbcL	3	CGGAAACCCCAAGAACAAGA	AACACCAGCTTTGAATCCGA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.294692	105840	106594	781-865	1.000000	82.3
atpB-rbcL_p4	atpB-rbcL	4	ATTTGAGCGATACGTCCCAC	AACACCAGCTTTGAATCCGA	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.334420	105840	106594	818-902	1.000000	82.0
atpB-rbcL_p5	atpB-rbcL	5	GATTTGAGCGATACGTCCCA	CCAGCTTTGAATCCGACACT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.405440	105840	106594	815-899	1.000000	81.4
ycf4-cemA_p1	ycf4-cemA	1	TGCGTGTATCCATTGAAGGG	TTTGGCCACTTCCAATTCGT	20	20	50.0	45.0	58.0	58.3	0.0	3.9	0.338452	111323	112174	983-987	1.000000	78.5
ycf4-cemA_p2	ycf4-cemA	2	GCGTGTATCCATTGAAGGGT	TTTGGCCACTTCCAATTCGT	20	20	50.0	45.0	58.0	58.3	0.0	3.9	0.339881	111323	112174	982-986	1.000000	78.5
ycf4-cemA_p3	ycf4-cemA	3	TGCGTGTATCCATTGAAGGG	CGAACCTGATTCACTCGTCA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.384655	111323	112174	953-957	1.000000	78.2
ycf4-cemA_p4	ycf4-cemA	4	GCGTGTATCCATTGAAGGGT	CGAACCTGATTCACTCGTCA	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.386084	111323	112174	952-956	1.000000	78.2
ycf4-cemA_p5	ycf4-cemA	5	TGCGTGTATCCATTGAAGGG	ACGAACCTGATTCACTCGTC	20	20	50.0	50.0	58.0	57.7	0.0	7.5	0.386255	111323	112174	954-958	0.916667	74.7
petA-psbJ_p1	petA-psbJ	1	GGGACCGGAACTTCTTGTTT	GGCAATGCAGTTCATCCAAC	20	20	50.0	50.0	58.0	58.0	9.5	7.8	0.023373	114141	115170	1672-1674	1.000000	80.9
petA-psbJ_p2	petA-psbJ	2	TCCCCATCCTTTCACCAGAT	GGCAATGCAGTTCATCCAAC	20	20	50.0	50.0	58.0	58.0	0.0	7.8	0.014808	114141	115170	1916-1918	1.000000	80.8
petA-psbJ_p3	petA-psbJ	3	CCTAATGTGGGTGGATTCGG	GGCAATGCAGTTCATCCAAC	20	20	55.0	50.0	58.0	58.0	0.0	7.8	0.033111	114141	115170	1608-1610	1.000000	80.8
petA-psbJ_p4	petA-psbJ	4	TCCCCATCCTTTCACCAGAT	AGTAACTGGCCGTTTCGATC	20	20	50.0	50.0	58.0	58.0	0.0	16.2	0.020349	114141	115170	2078-2080	1.000000	80.7
petA-psbJ_p5	petA-psbJ	5	GGGACCGGAACTTCTTGTTT	AGTAACTGGCCGTTTCGATC	20	20	50.0	50.0	58.0	58.0	9.5	16.2	0.028914	114141	115170	1834-1836	1.000000	80.7
psbE-petL_p1	psbE-petL	1	AATGTCAGCAAAGGAGCGTT	GCGTGGAAGGACTGAATGAA	20	20	45.0	50.0	58.4	58.2	0.0	0.0	0.582381	115937	117188	1325-1334	1.000000	76.8
psbE-petL_p2	psbE-petL	2	TAATGTCAGCAAAGGAGCGT	GCGTGGAAGGACTGAATGAA	20	20	45.0	50.0	57.5	58.2	0.0	0.0	0.664285	115937	117188	1326-1335	1.000000	76.2
psbE-petL_p3	psbE-petL	3	AATGTCAGCAAAGGAGCGTT	TAGGCGTGGAAGGACTGAAT	20	20	45.0	50.0	58.4	58.4	0.0	0.0	0.822288	115937	117188	1328-1337	1.000000	74.9
psbE-petL_p4	psbE-petL	4	AATGTCAGCAAAGGAGCGTT	ATAGTAGGCGTGGAAGGACT	20	20	45.0	50.0	58.4	57.5	0.0	0.0	0.846865	115937	117188	1332-1341	1.000000	74.7
psbE-petL_p5	psbE-petL	5	TAATGTCAGCAAAGGAGCGT	TAGGCGTGGAAGGACTGAAT	20	20	45.0	50.0	57.5	58.4	0.0	0.0	0.904192	115937	117188	1329-1338	1.000000	74.3