primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGACATGCTGCTTTTTCCAG	CGTTGCAATTGATGTTCGCT	20	20	50.0	45.0	58.0	58.0	0.0	10.3	0.059100	4176	4682	875-1110	0.500000	71.5
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGACATGCTGCTTTTTCCAG	ATTTCAACGAAGGCAGGTGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.045605	4176	4682	724-959	0.400000	67.6
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TGCTCGTGGTAAAAGCCTTT	ATTTCAACGAAGGCAGGTGT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.043084	4176	4682	1009-1151	0.200000	54.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TGCTCGTGGTAAAAGCCTTT	CGTTGCAATTGATGTTCGCT	20	20	45.0	45.0	58.0	58.0	0.0	10.3	0.056579	4176	4682	1160-1302	0.200000	54.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TGCTCGTGGTAAAAGCCTTT	TCGTTGCAATTGATGTTCGC	20	20	45.0	45.0	58.0	58.0	0.0	10.3	0.061928	4176	4682	1161-1303	0.200000	54.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5683	6107	490-739	0.800000	77.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	59.1	58.1	0.0	16.9	1.134559	5683	6107	493-742	0.800000	74.9
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	20	20	50.0	50.0	57.2	57.3	0.0	16.9	1.455196	5683	6107	494-743	0.800000	72.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CACGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	21	20	47.6	55.0	57.5	58.1	0.0	16.9	1.531720	5683	6107	492-741	0.800000	71.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CACGTTGCTTTCTACCACAT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	56.4	58.1	0.0	16.9	1.711328	5683	6107	492-741	0.800000	70.3
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	7609	8224	655-727	1.000000	63.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	7609	8224	654-726	1.000000	63.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	7609	8224	657-729	1.000000	62.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	7609	8224	656-728	1.000000	62.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.2	55.5	0.0	0.0	3.687191	7609	8224	709-781	1.000000	62.5
trnG-UCC_p1	trnG-UCC	1	CAATCCGACGCTTTAGTCCA	GGTCAGCGATTACGTGAGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.012413	8225	8965	2107-2224	0.900000	87.9
trnG-UCC_p2	trnG-UCC	2	AATCCGACGCTTTAGTCCAC	GGTCAGCGATTACGTGAGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013801	8225	8965	2106-2223	0.900000	87.9
trnG-UCC_p3	trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	GGTCAGCGATTACGTGAGTT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.159179	8225	8965	2161-2278	0.900000	86.7
trnG-UCC_p4	trnG-UCC	4	CTGGGCCTGGCTTCTTTTAT	GGTCAGCGATTACGTGAGTT	20	20	50.0	50.0	57.9	58.0	19.7	0.0	0.157803	8225	8965	1905-1959	0.400000	64.0
trnG-UCC_p5	trnG-UCC	5	CAATCCGACGCTTTAGTCCA	GAGGTCAGCGATTACGTGAG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.175751	8225	8965	2109-2115	0.200000	51.0
atpF_p1	atpF	1	ATACGAGCAATACCATCGCC	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.118673	10993	12298	2110-2177	0.800000	83.1
atpF_p2	atpF	2	CTGAAATTTCACCCCGACCA	ATTTCTGCCGCTTCCGTTAT	20	20	50.0	45.0	58.1	58.0	16.9	0.0	0.134100	10993	12298	2401-2422	0.700000	78.9
atpF_p3	atpF	3	AGCAATTCCTACCGTACCCT	TAATTGGGGGAGGGGGAATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.148688	10993	12298	1868-1877	0.200000	51.5
atpF_p4	atpF	4	CTGAAATTTCACCCCGACCA	TAATTGGGGGAGGGGGAATT	20	20	50.0	50.0	58.1	58.0	16.9	0.0	0.119889	10993	12298	2046-2055	0.200000	51.4
atpF_p5	atpF	5	ATACGAGCAATACCATCGCC	TAATTGGGGGAGGGGGAATT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104462	10993	12298	1810-1810	0.100000	43.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AGAGGTTCCCAGGGAATTCA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.9	58.1	24.7	0.0	0.141884	25795	26924	1432-1580	0.900000	86.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.109256	25795	26924	1605-1753	0.800000	83.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CGAGCCCATATCCTTGCTTT	TTTGCCCTATGACCCAATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.078462	25795	26924	2253-2271	0.300000	59.7
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AGAGGTTCCCAGGGAATTCA	TTTGCCCTATGACCCAATCG	20	20	50.0	50.0	57.9	57.9	24.7	0.0	0.111091	25795	26924	2080-2095	0.200000	51.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GCTCTCCGCTTACACAAGAA	TTTGCCCTATGACCCAATCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.131620	25795	26924	2450-2465	0.200000	50.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	27006	27906	904-1007	1.000000	90.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	27006	27906	965-1068	1.000000	88.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	27006	27906	922-1025	1.000000	87.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGTGTCGCCTGATGATAAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.7	58.2	1.7	0.0	0.476931	27006	27906	948-989	0.400000	63.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGTGTCGCCTGATGATAAA	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	57.7	57.6	1.7	0.0	0.676446	27006	27906	1009-1050	0.400000	61.3
atpB-rbcL_p1	atpB-rbcL	1	TCCATGAGCTTTGGCAATGT	CGGTCTCTCCAACGCATAAA	20	20	45.0	50.0	58.1	58.0	13.8	0.0	0.077095	53177	53931	1996-2024	1.000000	91.4
atpB-rbcL_p2	atpB-rbcL	2	ATTGTATAAAGGCAGGCGCA	CGGTCTCTCCAACGCATAAA	20	20	45.0	50.0	57.9	58.0	10.8	0.0	0.055917	53177	53931	1838-1866	0.900000	87.6
atpB-rbcL_p3	atpB-rbcL	3	ATTGTATAAAGGCAGGCGCA	CCCGTCAGTCCATACAGTTG	20	20	45.0	55.0	57.9	58.0	10.8	0.0	0.061479	53177	53931	1407-1435	0.900000	87.5
atpB-rbcL_p4	atpB-rbcL	4	TCCATGAGCTTTGGCAATGT	CCCGTCAGTCCATACAGTTG	20	20	45.0	55.0	58.1	58.0	13.8	0.0	0.082658	53177	53931	1565-1593	0.900000	87.3
atpB-rbcL_p5	atpB-rbcL	5	ATTGTATAAAGGCAGGCGCA	CAGGCTCAATGTGGTAGCAT	20	20	45.0	50.0	57.9	58.0	10.8	0.0	0.099778	53177	53931	1456-1484	0.900000	87.2
rbcL-accD_p1	rbcL-accD	1	GCAAATACGAGTCTGGCTCA	TTGAACCCACAAATGCCTGT	20	20	50.0	45.0	58.0	58.2	10.3	0.0	0.223208	55381	56105	1955-2037	0.900000	86.2
rbcL-accD_p2	rbcL-accD	2	TGCAAATACGAGTCTGGCTC	TTGAACCCACAAATGCCTGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.223208	55381	56105	1956-2038	0.900000	86.2
rbcL-accD_p3	rbcL-accD	3	CTGCAAATACGAGTCTGGCT	TTGAACCCACAAATGCCTGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.228799	55381	56105	1957-2039	0.900000	86.2
rbcL-accD_p4	rbcL-accD	4	GCCGTAGCTAATCGAGTAGC	TTGAACCCACAAATGCCTGT	20	20	55.0	45.0	58.0	58.2	31.7	0.0	0.246641	55381	56105	1532-1614	0.900000	86.0
rbcL-accD_p5	rbcL-accD	5	ATATGCCTGCTTTGACCGAG	TTGAACCCACAAATGCCTGT	20	20	50.0	45.0	58.0	58.2	0.0	0.0	0.254021	55381	56105	1624-1706	0.900000	86.0
psbE-petL_p1	psbE-petL	1	TCTCCTGTGCTTCCAGACAT	AGTAGAAAACCGAAATAACTAGT	20	23	50.0	30.4	58.4	53.2	0.9	0.0	8.150663	63522	64601	909-1208	1.000000	52.0
psbE-petL_p2	psbE-petL	2	TCTCCTGTGCTTCCAGACAT	ACCGAAATAACTAGTTATAGTAAGCA	20	26	50.0	30.8	58.4	56.1	0.9	24.9	8.271440	63522	64601	901-1200	1.000000	52.0
psbE-petL_p3	psbE-petL	3	TCTCCTGTGCTTCCAGACAT	AGTAGAAAACCGAAATAACTAGTT	20	24	50.0	29.2	58.4	54.0	0.9	13.7	8.351873	63522	64601	909-1208	1.000000	52.0
psbE-petL_p4	psbE-petL	4	TATCGGCAAAAGAACGCTCT	AGTAGAAAACCGAAATAACTAGT	20	23	45.0	30.4	57.6	53.2	0.0	0.0	8.180068	63522	64601	1152-1152	0.100000	4.0
psbE-petL_p5	psbE-petL	5	TATCGGCAAAAGAACGCTCT	ACCGAAATAACTAGTTATAGTAAGCA	20	26	45.0	30.8	57.6	56.1	0.0	24.9	8.300845	63522	64601	1144-1144	0.100000	4.0
rpl20-rps12_p1	rpl20-rps12	1	TTGGTTCTACGTCTTCGAGC	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	57.7	58.7	0.0	0.0	1.073744	67773	68582	837-899	1.000000	83.4
rpl20-rps12_p2	rpl20-rps12	2	TTGGTTCTACGTCTTCGAGC	TCAGCGACGAGGAACATGTA	20	20	50.0	50.0	57.7	58.8	0.0	0.0	1.172468	67773	68582	838-900	1.000000	82.6
rpl20-rps12_p3	rpl20-rps12	3	TGGTTCTACGTCTTCGAGCT	CAGCGACGAGGAACATGTAC	20	20	50.0	55.0	58.5	58.7	0.0	0.0	1.199412	67773	68582	836-898	1.000000	82.4
rpl20-rps12_p4	rpl20-rps12	4	TTGGTTCTACGTCTTCGAGC	AGCGACGAGGAACATGTACT	20	20	50.0	50.0	57.7	58.8	0.0	0.0	1.168127	67773	68582	836-898	0.900000	78.7
rpl20-rps12_p5	rpl20-rps12	5	TTGGTTCTACGTCTTCGAGC	GCGACGAGGAACATGTACTA	20	20	50.0	50.0	57.7	57.2	0.0	0.0	1.181296	67773	68582	835-897	0.800000	74.5
clpP_p1	clpP	1	CTGAACGAGTCGCACATACA	ACGAACATACCCTGTCTCCA	20	20	50.0	50.0	58.0	58.1	13.6	0.0	0.089790	68850	70814	2837-2914	1.000000	91.3
clpP_p2	clpP	2	CTGAACGAGTCGCACATACA	CGTATAGAGCCATCGAACCG	20	20	50.0	55.0	58.0	58.0	13.6	0.0	0.057728	68850	70814	2773-2849	0.800000	83.5
clpP_p3	clpP	3	ATGATCTGAACGAGTCGCAC	CGTATAGAGCCATCGAACCG	20	20	50.0	55.0	58.1	58.0	13.6	0.0	0.120283	68850	70814	2778-2854	0.800000	83.0
clpP_p4	clpP	4	GATCTGAACGAGTCGCACAT	CGTATAGAGCCATCGAACCG	20	20	50.0	55.0	58.1	58.0	13.6	0.0	0.120283	68850	70814	2776-2852	0.800000	83.0
clpP_p5	clpP	5	ACATGATCTGAACGAGTCGC	CGTATAGAGCCATCGAACCG	20	20	50.0	55.0	58.1	58.0	13.6	0.0	0.120283	68850	70814	2780-2856	0.400000	65.0
rpl16_p1	rpl16	1	TTTCTGGCTCCGCTGTTATC	ATGGATCCGGGAAGGTAGAG	20	20	50.0	55.0	58.0	58.0	0.0	7.4	0.041080	79977	81270	1720-1891	0.800000	83.7
rpl16_p2	rpl16	2	AGCGTCGATTACTAGCTCCT	ATGGATCCGGGAAGGTAGAG	20	20	50.0	55.0	58.0	58.0	0.0	7.4	0.046813	79977	81270	1761-1932	0.800000	83.6
rpl16_p3	rpl16	3	TGTTTCAATGCAAAGGCGAC	ATGGATCCGGGAAGGTAGAG	20	20	45.0	55.0	57.9	58.0	0.0	7.4	0.149645	79977	81270	1652-1807	0.600000	74.8
rpl16_p4	rpl16	4	TTTCTGGCTCCGCTGTTATC	GCCGAATTTATAGCCGGACA	20	20	50.0	50.0	58.0	58.1	0.0	5.0	0.150478	79977	81270	1869-2024	0.400000	63.8
rpl16_p5	rpl16	5	TTTCTGGCTCCGCTGTTATC	TGCCGAATTTATAGCCGGAC	20	20	50.0	50.0	58.0	58.1	0.0	5.0	0.150478	79977	81270	1870-2025	0.400000	63.8
ndhF-rpl32_p1	ndhF-rpl32	1	CTCCAACGCGATTAGTCACA	AATGCTGCCCAATAGCCTTT	20	20	50.0	45.0	58.0	58.1	15.9	0.0	0.139137	112150	113064	1415-1649	0.700000	78.9
ndhF-rpl32_p2	ndhF-rpl32	2	CTCCAACGCGATTAGTCACA	AAAAGCTTTCAATGCTGCCC	20	20	50.0	45.0	58.0	57.8	15.9	13.1	0.183564	112150	113064	1425-1659	0.700000	78.5
ndhF-rpl32_p3	ndhF-rpl32	3	CTCCAACGCGATTAGTCACA	ATGCTGCCCAATAGCCTTTT	20	20	50.0	45.0	58.0	58.1	15.9	0.0	0.139137	112150	113064	1490-1648	0.300000	62.1
ndhF-rpl32_p4	ndhF-rpl32	4	AGATCCTTTGTTGCCTTGGG	AATGCTGCCCAATAGCCTTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.201068	112150	113064	1108-1200	0.200000	54.8
ndhF-rpl32_p5	ndhF-rpl32	5	AGATCCTTTGTTGCCTTGGG	ATGCTGCCCAATAGCCTTTT	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.201068	112150	113064	1107-1199	0.200000	54.8
ycf1_p1	ycf1	1	GCACAAGTTATGCACTTCCT	GGGGAATGGAAACCGAACTT	20	20	45.0	50.0	56.3	58.1	0.1	0.0	1.750278	126383	126502	301-361	0.300000	45.3
ycf1_p2	ycf1	2	GCACAAGTTATGCACTTCCT	AACCGTTTGGGGAATGGAAA	20	20	45.0	45.0	56.3	57.9	0.1	4.1	1.759118	126383	126502	309-369	0.300000	45.2
ycf1_p3	ycf1	3	GCACAAGTTATGCACTTCCT	AATAGCCGAAGCCTGGGATA	20	20	45.0	50.0	56.3	58.3	0.1	0.0	1.939932	126383	126502	672-744	0.300000	42.6
ycf1_p4	ycf1	4	GCACAAGTTATGCACTTCCT	TTTGGAACGAACCGAACCTT	20	20	45.0	45.0	56.3	58.0	0.1	0.0	1.710769	126383	126502	930-936	0.200000	35.1
ycf1_p5	ycf1	5	GCACAAGTTATGCACTTCCT	TTTTGGAACGAACCGAACCT	20	20	45.0	45.0	56.3	58.0	0.1	0.0	1.710769	126383	126502	931-937	0.200000	35.1