primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	7.8	16.9	0.156387	6271	6720	540-765	1.000000	81.5
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6271	6720	523-748	1.000000	81.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	7.8	16.9	0.285346	6271	6720	541-766	1.000000	80.5
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	6271	6720	538-763	1.000000	79.2
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	6271	6720	537-762	1.000000	79.2
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9466	9629	240-245	1.000000	67.3
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9466	9629	243-248	1.000000	67.0
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9466	9629	246-251	1.000000	66.5
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9466	9629	249-254	1.000000	66.3
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9466	9629	244-249	1.000000	64.8
atpH-atpI_p1	atpH-atpI	1	AACGGAAGCAGCAGAAATCA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	13233	14264	1107-1117	1.000000	80.0
atpH-atpI_p2	atpH-atpI	2	ACGGAAGCAGCAGAAATCAA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.1	57.9	0.0	0.0	0.236520	13233	14264	1106-1116	1.000000	80.0
atpH-atpI_p3	atpH-atpI	3	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCCGCAG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.347127	13233	14264	1124-1134	1.000000	79.1
atpH-atpI_p4	atpH-atpI	4	AACGGAAGCAGCAGAAATCA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	0.0	0.0	0.362752	13233	14264	1156-1166	1.000000	78.9
atpH-atpI_p5	atpH-atpI	5	ACGGAAGCAGCAGAAATCAA	TCCCTGTCATGTTCCTTGGA	20	20	45.0	50.0	58.1	58.3	0.0	0.0	0.362752	13233	14264	1155-1165	1.000000	78.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	27723	28483	871-882	1.000000	81.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.087498	27723	28483	869-880	1.000000	81.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	20	20	50.0	50.0	57.9	58.0	14.3	0.0	0.143966	27723	28483	923-934	1.000000	80.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	20	20	50.0	50.0	57.9	58.0	14.3	0.0	0.143966	27723	28483	921-932	1.000000	80.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.162414	27723	28483	872-883	1.000000	80.4
petN-psbM_p1	petN-psbM	1	ACTCGTAGTGTGGGGAAGAA	TGCATTTATCGCTACTGCACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.100295	28574	29151	706-717	1.000000	72.9
petN-psbM_p2	petN-psbM	2	TCGTAGTGTGGGGAAGAAGT	TGCATTTATCGCTACTGCACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.100295	28574	29151	704-715	1.000000	72.9
petN-psbM_p3	petN-psbM	3	CGTAGTGTGGGGAAGAAGTG	TGCATTTATCGCTACTGCACT	20	21	55.0	42.9	57.9	58.1	0.0	0.0	1.175211	28574	29151	703-714	1.000000	72.3
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCATTTATCGCTACTGCACT	20	21	50.0	42.9	58.2	58.1	0.0	0.0	1.246273	28574	29151	758-769	1.000000	71.6
petN-psbM_p5	petN-psbM	5	TCGTAGTGTGGGGAAGAAGT	TGCATTTATCGCTACTGCAC	20	20	50.0	45.0	58.0	56.6	0.0	0.0	1.401385	28574	29151	704-715	1.000000	70.5
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	35812	35979	228-234	1.000000	82.3
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	58.1	58.0	0.0	4.2	0.096882	35812	35979	229-235	1.000000	82.3
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	ATGCGGATATGGTCGAATGG	CTTGAGGTCACGGGTTCAAA	20	20	50.0	50.0	57.9	58.0	0.0	4.2	0.137348	35812	35979	269-275	1.000000	81.5
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	AGAAGATGCGGGTTCGATTC	TGACTTTATCTCTTCGGCGC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.136105	35812	35979	285-291	1.000000	81.4
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	GAGAAGATGCGGGTTCGATT	TGACTTTATCTCTTCGGCGC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.136105	35812	35979	286-292	1.000000	81.4
psaA-ycf3_p1	psaA-ycf3	1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.136672	41126	41881	947-989	1.000000	80.4
psaA-ycf3_p2	psaA-ycf3	2	TGAGAAATGACCCGGTTTGG	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	58.1	58.0	0.0	18.7	0.140786	41126	41881	929-971	1.000000	80.4
psaA-ycf3_p3	psaA-ycf3	3	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.356647	41126	41881	943-985	1.000000	78.6
psaA-ycf3_p4	psaA-ycf3	4	TGAGAAATGACCCGGTTTGG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.357875	41126	41881	945-987	1.000000	78.6
psaA-ycf3_p5	psaA-ycf3	5	TGAGAAATGACCCGGTTTGG	AGGCTATAGCGCTTACTCCT	20	20	50.0	50.0	58.1	57.7	0.0	18.7	0.391786	41126	41881	923-965	1.000000	78.4
rbcL-accD_p1	rbcL-accD	1	CCAGTGGATACTTTGGATGTGT	ATAGAGTTTCCTTGCCCCCT	22	20	45.5	50.0	58.0	58.0	1.7	0.0	2.057449	56226	56863	712-715	1.000000	66.7
rbcL-accD_p2	rbcL-accD	2	CCAGTGGATACTTTGGATGTGT	CATAGAGTTTCCTTGCCCCC	22	20	45.5	55.0	58.0	57.9	1.7	0.0	2.103121	56226	56863	713-716	1.000000	66.3
rbcL-accD_p3	rbcL-accD	3	CCAGTGGATACTTTGGATGTGT	CCATAGAGTTTCCTTGCCCC	22	20	45.5	55.0	58.0	57.9	1.7	0.0	2.103121	56226	56863	714-717	1.000000	66.3
rbcL-accD_p4	rbcL-accD	4	CCAGTGGATACTTTGGATGTGT	ATACCTGCGCTCTAACTCCT	22	20	45.5	50.0	58.0	57.9	1.7	9.7	2.119257	56226	56863	767-770	1.000000	66.0
rbcL-accD_p5	rbcL-accD	5	CCAGTGGATACTTTGGATGTGT	AGAGTTTCCTTGCCCCCTAT	22	20	45.5	50.0	58.0	58.0	1.7	0.0	2.057449	56226	56863	713-713	0.666667	52.2
petL-petG_p1	petL-petG	1	TGGTCTGAGCAAGATACGAC	CAACTGATCACCACGCCTAT	20	20	50.0	50.0	57.1	57.7	7.1	0.0	1.257378	65491	65683	311-326	1.000000	75.3
petL-petG_p2	petL-petG	2	TGGTCTGAGCAAGATACGAC	AAGGTCCAACTGATCACCAC	20	20	50.0	50.0	57.1	57.4	7.1	0.0	1.510076	65491	65683	317-332	1.000000	73.1
petL-petG_p3	petL-petG	3	TTGGTCTGAGCAAGATACGAC	CAACTGATCACCACGCCTAT	21	20	47.6	50.0	57.8	57.7	17.5	0.0	1.548138	65491	65683	312-327	1.000000	72.9
petL-petG_p4	petL-petG	4	GGTCTGAGCAAGATACGACT	CAACTGATCACCACGCCTAT	20	20	50.0	50.0	56.8	57.7	1.3	0.0	1.553436	65491	65683	310-325	1.000000	72.9
petL-petG_p5	petL-petG	5	TTGGTCTGAGCAAGATACGAC	AAGGTCCAACTGATCACCAC	21	20	47.6	50.0	57.8	57.4	17.5	0.0	1.800836	65491	65683	318-333	1.000000	70.8
trnI-GAU_p1	trnI-GAU	1	AGAGAGGGATGGGGTTTCTC	AAGTGGAGCATGCATGAAGT	20	20	55.0	45.0	58.1	57.8	0.0	30.6	0.324828	101089	102104	1142-1144	1.000000	78.0
trnI-GAU_p2	trnI-GAU	2	AGAGGGATGGGGTTTCTCTC	AAGTGGAGCATGCATGAAGT	20	20	55.0	45.0	58.1	57.8	0.0	30.6	0.324828	101089	102104	1140-1142	1.000000	78.0
trnI-GAU_p3	trnI-GAU	3	GAGAGGGATGGGGTTTCTCT	AAGTGGAGCATGCATGAAGT	20	20	55.0	45.0	58.1	57.8	0.0	30.6	0.324828	101089	102104	1141-1143	1.000000	78.0
trnI-GAU_p4	trnI-GAU	4	GATGGGGTTTCTCTCGCTTT	AAGTGGAGCATGCATGAAGT	20	20	50.0	45.0	57.9	57.8	0.0	30.6	0.326593	101089	102104	1135-1137	1.000000	78.0
trnI-GAU_p5	trnI-GAU	5	AGAGGGATGGGGTTTCTCTC	CTTTTTCTTTTCCCTGGCGC	20	20	55.0	50.0	58.1	58.2	0.0	0.0	0.330294	101089	102104	1100-1102	1.000000	78.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGAAAAGGAAAGGGTGTCG	AAAAATAGCACTGGGCCCAA	20	20	50.0	45.0	58.0	58.0	0.0	28.3	0.047058	112264	113137	1511-1518	1.000000	83.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCAGTCTCTTTCCCCGAGTT	AAAAATAGCACTGGGCCCAA	20	20	50.0	45.0	58.0	58.0	0.0	28.3	0.033840	112264	113137	2071-2079	1.000000	82.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TTCAGTCTCTTTCCCCGAGT	AAAAATAGCACTGGGCCCAA	20	20	50.0	45.0	58.0	58.0	0.0	28.3	0.033840	112264	113137	2072-2080	1.000000	82.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCAGTCTCTTTCCCCGAGTT	TGGGCCCAATTATTGTGCTT	20	20	50.0	45.0	58.0	58.1	0.0	26.3	0.061323	112264	113137	2060-2068	1.000000	82.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTCAGTCTCTTTCCCCGAGT	TGGGCCCAATTATTGTGCTT	20	20	50.0	45.0	58.0	58.1	0.0	26.3	0.061323	112264	113137	2061-2069	1.000000	82.5
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	AGAATTGAAACGATCGAAAAAGA	20	23	50.0	30.4	57.9	55.2	0.0	24.3	5.920680	113218	113349	248-255	1.000000	46.9
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	AAATTGGTAGACACGCTGCT	AGAATTGAAACGATCGAAAAAGA	20	23	45.0	30.4	57.8	55.2	0.0	24.3	5.981407	113218	113349	255-262	1.000000	46.8
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GGTAGACACGCTGCTCTTAG	AGAATTGAAACGATCGAAAAAGA	20	23	55.0	30.4	57.8	55.2	0.0	24.3	5.992698	113218	113349	250-257	1.000000	46.8
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	GTAGACACGCTGCTCTTAGG	AGAATTGAAACGATCGAAAAAGA	20	23	55.0	30.4	57.8	55.2	0.0	24.3	5.992698	113218	113349	249-256	1.000000	46.8
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	AATTGGTAGACACGCTGCTC	AGAATTGAAACGATCGAAAAAGA	20	23	50.0	30.4	58.3	55.2	0.0	24.3	6.069549	113218	113349	254-261	1.000000	46.8
ndhD_p1	ndhD	1	TGGGTCCCTTTTCTTTGGTG	CCCTGGGATGGATGTAAAGC	20	20	50.0	55.0	57.9	58.0	10.0	7.0	0.093772	114545	116074	2436-2439	1.000000	80.8
ndhD_p2	ndhD	2	TTGGAGTTGGGACCCAAAAG	CCCTGGGATGGATGTAAAGC	20	20	50.0	55.0	57.9	58.0	27.9	7.0	0.093772	114545	116074	2223-2226	1.000000	80.8
ndhD_p3	ndhD	3	CTAACGAAGCATGGGGATCC	CCCTGGGATGGATGTAAAGC	20	20	55.0	55.0	58.1	58.0	1.9	7.0	0.128882	114545	116074	2245-2248	1.000000	80.5
ndhD_p4	ndhD	4	TTGGTGTGAACTGGCAAGAA	CCCTGGGATGGATGTAAAGC	20	20	45.0	55.0	57.9	58.0	0.0	7.0	0.149416	114545	116074	1939-1942	1.000000	80.2
ndhD_p5	ndhD	5	TTTGGTGTGAACTGGCAAGA	CCCTGGGATGGATGTAAAGC	20	20	45.0	55.0	57.9	58.0	0.0	7.0	0.149416	114545	116074	1940-1943	1.000000	80.2
ndhD-psaC_p1	ndhD-psaC	1	TCGTAGTAAAGACAAGATACACT	TAGGGAATGAAACAACCCGC	23	20	34.8	50.0	54.6	58.2	0.0	0.0	6.537734	116075	116171	169-169	1.000000	46.2
ndhD-psaC_p2	ndhD-psaC	2	AGGAAAATGATTCGTAGTAAAGACA	TAGGGAATGAAACAACCCGC	25	20	32.0	50.0	56.6	58.2	0.0	0.0	6.620508	116075	116171	180-180	1.000000	45.8
ndhD-psaC_p3	ndhD-psaC	3	AGAAAGGAAAATGATTCGTAGT	TAGGGAATGAAACAACCCGC	22	20	31.8	50.0	53.5	58.2	0.0	0.0	6.697093	116075	116171	184-184	1.000000	45.7
ndhD-psaC_p4	ndhD-psaC	4	TCGTAGTAAAGACAAGATACACT	AAGAGATGTGAATCCGCCTG	23	20	34.8	50.0	54.6	57.7	0.0	0.0	6.689069	116075	116171	221-221	1.000000	44.7
ndhD-psaC_p5	ndhD-psaC	5	AGGAAAATGATTCGTAGTAAAGACA	AAGAGATGTGAATCCGCCTG	25	20	32.0	50.0	56.6	57.7	0.0	0.0	6.771843	116075	116171	232-232	1.000000	44.5
rps15-ycf1_p1	rps15-ycf1	1	CAGCAGTCGTTGACGTTTTC	GCCGGTTGGGAAGAGTTTAA	20	20	50.0	50.0	58.0	58.1	3.2	1.9	0.104943	122396	122809	1132-1139	1.000000	83.8
rps15-ycf1_p2	rps15-ycf1	2	TCCCAACGCGTTACATAAGG	GCCGGTTGGGAAGAGTTTAA	20	20	50.0	50.0	58.0	58.1	26.1	1.9	0.112691	122396	122809	1523-1530	1.000000	82.9
rps15-ycf1_p3	rps15-ycf1	3	CAGCAGTCGTTGACGTTTTC	CCCATCCGTTTGTCTGGAAA	20	20	50.0	50.0	58.0	58.1	3.2	0.0	0.100902	122396	122809	1071-1074	0.666667	69.6
rps15-ycf1_p4	rps15-ycf1	4	AGTCGTTGACGTTTTCCCAA	CCCATCCGTTTGTCTGGAAA	20	20	45.0	50.0	58.0	58.1	3.2	0.0	0.122716	122396	122809	1067-1070	0.666667	69.4
rps15-ycf1_p5	rps15-ycf1	5	TCCCAACGCGTTACATAAGG	CCCATCCGTTTGTCTGGAAA	20	20	50.0	50.0	58.0	58.1	26.1	0.0	0.108650	122396	122809	1462-1465	0.666667	68.7
ycf1_p1	ycf1	1	GAATACCGTCCCTCAACCAC	ACTCGAAACAAGAACTCGGT	20	20	55.0	45.0	58.0	57.1	0.0	0.0	0.904841	126138	126257	1238-1247	1.000000	76.4
ycf1_p2	ycf1	2	GTGCCAAGGTTTCAGACAGA	ACTCGAAACAAGAACTCGGT	20	20	50.0	45.0	58.0	57.1	0.0	0.0	0.917575	126138	126257	1279-1288	1.000000	76.2
ycf1_p3	ycf1	3	AAGTTCGTTTTCGGGGTGAA	ACTCGAAACAAGAACTCGGT	20	20	45.0	45.0	58.0	57.1	0.0	0.0	0.926145	126138	126257	1406-1415	1.000000	76.2
ycf1_p4	ycf1	4	GCAACAGGAATATACGCCCA	ACTCGAAACAAGAACTCGGT	20	20	50.0	45.0	58.0	57.1	0.0	0.0	0.923100	126138	126257	1115-1115	0.333333	45.9
ycf1_p5	ycf1	5	TGCAACAGGAATATACGCCC	ACTCGAAACAAGAACTCGGT	20	20	50.0	45.0	58.0	57.1	0.0	0.0	0.923100	126138	126257	1116-1116	0.333333	45.9