primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6365	7783	1386-1492	1.000000	89.1
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6365	7783	1397-1503	1.000000	88.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6365	7783	1390-1496	1.000000	84.4
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ATGATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.2	58.1	0.0	16.9	0.844354	6365	7783	1398-1504	1.000000	84.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6365	7783	1378-1484	1.000000	84.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	TGAACAAAACAGAAAGACCCCT	20	22	50.0	40.9	58.0	58.0	0.0	0.0	2.028568	9334	10244	1025-1424	1.000000	71.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	TGAACAAAACAGAAAGACCCCT	20	22	50.0	40.9	58.0	58.0	0.0	0.0	2.029956	9334	10244	1024-1423	1.000000	71.1
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGCAATCCGACGCTTTAGTC	TGAACAAAACAGAAAGACCCCT	20	22	50.0	40.9	58.4	58.0	0.0	0.0	2.382408	9334	10244	1027-1426	1.000000	68.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGACGCTTTAGTCCACTCAG	TGAACAAAACAGAAAGACCCCT	20	22	55.0	40.9	58.4	58.0	0.0	0.0	2.405125	9334	10244	1020-1419	1.000000	68.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GCTTTAGTCCACTCAGCCAT	TGAACAAAACAGAAAGACCCCT	20	22	50.0	40.9	57.6	58.0	0.9	0.0	2.433934	9334	10244	1016-1415	1.000000	67.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGAACTAGAATAAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	24	20	37.5	50.0	57.4	58.5	0.0	5.4	5.090485	31801	32983	1301-1341	1.000000	47.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GGAACTAGAATAAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	23	20	39.1	50.0	56.0	58.5	0.0	5.4	5.448359	31801	32983	1300-1340	1.000000	47.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGAACTAGAATAAAGAGTGCAGT	GTTCAATTGGTCAGAGCACC	24	20	37.5	50.0	57.4	57.0	0.0	5.4	5.610638	31801	32983	1302-1342	1.000000	47.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	ACTAGAATAAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	23	20	39.1	50.0	55.9	58.5	0.0	5.4	5.617523	31801	32983	1297-1337	1.000000	47.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAGAATAAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	23	20	39.1	50.0	55.8	58.5	0.0	5.4	5.711699	31801	32983	1301-1341	1.000000	47.3
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	33058	33437	465-474	1.000000	80.9
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	33058	33437	464-473	1.000000	80.7
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.889785	33058	33437	498-507	1.000000	79.9
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	ATCCTAGTTCGGGACTGACG	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	58.6	58.4	0.0	0.0	0.960931	33058	33437	518-527	1.000000	79.0
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	ATCCTAGTTCGGGACTGACG	GGGACGGACTGTAAATTCGT	20	20	55.0	50.0	58.6	57.6	0.0	0.0	0.984608	33058	33437	517-526	1.000000	78.9
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.165150	33661	34462	887-943	1.000000	89.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.8	58.1	0.0	0.0	0.223247	33661	34462	866-922	1.000000	88.8
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.245401	33661	34462	863-919	1.000000	88.6
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	33661	34462	849-905	1.000000	88.5
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	20	20	55.0	55.0	57.8	58.2	0.0	0.0	0.310083	33661	34462	884-940	1.000000	87.9
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	20	20	55.0	55.0	58.1	58.0	7.6	5.0	0.067776	34535	35646	1225-1254	1.000000	86.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	20	20	50.0	55.0	58.1	58.0	0.0	5.0	0.089930	34535	35646	1228-1257	1.000000	86.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	CACAAAAACGAAACGGTCCC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	34535	35646	1234-1263	1.000000	85.1
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	CCACAAAAACGAAACGGTCC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	34535	35646	1235-1264	1.000000	85.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.233981	34535	35646	1236-1265	1.000000	85.1
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	AGTTTTTCTGGTAGCTATCCT	CGTCTTCTCCTTGGCAAAGA	21	20	38.1	50.0	53.9	57.8	0.4	1.1	5.223103	38844	39419	655-672	1.000000	48.5
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TGGATTAGTTTTTCTGGTAGCT	CGTCTTCTCCTTGGCAAAGA	22	20	36.4	50.0	55.1	57.8	0.0	1.1	5.090937	38844	39419	661-678	1.000000	48.4
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TGGATTGGATTAGTTTTTCTGGT	CGTCTTCTCCTTGGCAAAGA	23	20	34.8	50.0	56.1	57.8	0.0	1.1	5.098065	38844	39419	666-683	1.000000	48.4
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TTGGATTAGTTTTTCTGGTAGCT	CGTCTTCTCCTTGGCAAAGA	23	20	34.8	50.0	55.8	57.8	0.0	1.1	5.333981	38844	39419	662-679	1.000000	48.4
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GGATTGGATTAGTTTTTCTGGTAGC	CGTCTTCTCCTTGGCAAAGA	25	20	40.0	50.0	58.2	57.8	0.0	1.1	5.373407	38844	39419	665-682	1.000000	48.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CCATTTATCCGAGATCTAGGCA	CTACACCTTCAACCACTCGG	22	20	45.5	55.0	57.2	57.9	16.6	0.0	2.888700	47393	48050	700-711	1.000000	65.4
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCATTTATCCGAGATCTAGGCA	GCAGTTCCAATGCTACACCT	22	20	45.5	50.0	57.2	58.2	16.6	0.0	2.982030	47393	48050	712-723	1.000000	64.5
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCCATTTATCCGAGATCTAGGC	CTACACCTTCAACCACTCGG	22	20	45.5	55.0	56.9	57.9	16.6	0.0	3.155669	47393	48050	701-712	1.000000	63.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TCCATTTATCCGAGATCTAGGC	GCAGTTCCAATGCTACACCT	22	20	45.5	50.0	56.9	58.2	16.6	0.0	3.248998	47393	48050	713-724	1.000000	62.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCATTTATCCGAGATCTAGGCA	TGCTACACCTTCAACCACTC	22	20	45.5	50.0	57.2	57.5	16.6	0.0	3.352192	47393	48050	702-713	1.000000	61.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	49535	50472	922-988	1.000000	88.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	49535	50472	953-1019	1.000000	87.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	49535	50472	930-996	1.000000	87.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	49535	50472	961-1027	1.000000	87.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	ATCCAATCAAGTCCGTAGCG	20	20	55.0	50.0	57.9	57.8	0.0	0.0	0.357808	49535	50472	945-1011	1.000000	86.2
petA-psbJ_p1	petA-psbJ	1	GGGCGTCAAGTGGTTGATAT	TCCCTATTCATTGCGGGTTG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.089721	67865	68807	1897-1907	1.000000	84.3
petA-psbJ_p2	petA-psbJ	2	GGGCGTCAAGTGGTTGATAT	TGGACTAGCTGTACCTACCG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.086831	67865	68807	1666-1671	0.666667	70.9
petA-psbJ_p3	petA-psbJ	3	GGGCGTCAAGTGGTTGATAT	GGACTAGCTGTACCTACCGT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.088275	67865	68807	1665-1670	0.666667	70.9
petA-psbJ_p4	petA-psbJ	4	CAAGATCCATTACGCGTCCA	TGGACTAGCTGTACCTACCG	20	20	50.0	55.0	58.1	58.0	18.6	0.0	0.113855	67865	68807	1528-1528	0.333333	52.4
petA-psbJ_p5	petA-psbJ	5	CAAGATCCATTACGCGTCCA	GGACTAGCTGTACCTACCGT	20	20	50.0	55.0	58.1	58.0	18.6	0.0	0.115300	67865	68807	1527-1527	0.333333	52.4
ycf1_p1	ycf1	1	TCGCTTGACTTGATTGGTGT	GATACCTGGTCCAATACGCA	20	20	45.0	50.0	57.7	57.1	0.0	0.6	1.157251	131062	131181	451-451	0.333333	44.1
ycf1_p2	ycf1	2	TCGCTTGACTTGATTGGTGT	TGATACCTGGTCCAATACGC	20	20	45.0	50.0	57.7	57.1	0.0	0.6	1.157251	131062	131181	452-452	0.333333	44.1
ycf1_p3	ycf1	3	TCGCTTGACTTGATTGGTGT	AGCGAATTCATTGATGGGTCA	20	21	45.0	42.9	57.7	58.0	0.0	6.2	1.257940	131062	131181	761-761	0.333333	43.3
ycf1_p4	ycf1	4	TCGCTTGACTTGATTGGTGT	GGAGCGAATTCATTGATGGG	20	20	45.0	50.0	57.7	56.9	0.0	6.2	1.328140	131062	131181	763-763	0.333333	42.7
ycf1_p5	ycf1	5	TCGCTTGACTTGATTGGTGT	TTGATACCTGGTCCAATACGC	20	21	45.0	47.6	57.7	57.8	0.0	0.6	1.442704	131062	131181	453-453	0.333333	41.8
ycf2_p1	ycf2	1	CAAAGATCTTGAGCGACCGA	AGTTCTCCACCCTAACGACA	20	20	50.0	50.0	58.0	58.0	9.2	0.0	0.009079	153377	153496	1330-1354	0.666667	73.2
ycf2_p2	ycf2	2	TCCGGCAGAACAACTCAAAA	AGTTCTCCACCCTAACGACA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.050932	153377	153496	1310-1334	0.666667	72.9
ycf2_p3	ycf2	3	GTGCTACAAGATCTCGTGCA	AGTTCTCCACCCTAACGACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009079	153377	153496	981-981	0.333333	53.3
ycf2_p4	ycf2	4	AGATCTCGTGCATTGGAACC	AGTTCTCCACCCTAACGACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.035208	153377	153496	973-973	0.333333	53.1
ycf2_p5	ycf2	5	GTGCTACAAGATCTCGTGCA	CCGCTTATGGGGTCAAATCA	20	20	50.0	50.0	58.0	57.9	0.0	3.7	0.055043	153377	153496	1242-1242	0.333333	52.9