primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	GGTATGGATGAATCCCTCGC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	6.9	7.6	0.414250	4374	5187	975-1042	1.000000	88.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4374	5187	920-987	1.000000	88.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	4374	5187	919-986	1.000000	88.0
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	4374	5187	1004-1071	1.000000	87.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.554139	4374	5187	1003-1070	1.000000	87.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6303	7261	1011-1046	1.000000	89.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	6303	7261	1015-1050	1.000000	85.1
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	6303	7261	1041-1076	1.000000	84.8
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	6303	7261	1003-1038	1.000000	84.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AGTCGCACGTTGCTTTCTAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.9	58.1	8.1	16.9	0.938588	6303	7261	1010-1045	1.000000	84.1
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9459	10267	863-893	1.000000	89.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9459	10267	881-911	1.000000	86.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9459	10267	862-892	1.000000	83.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9459	10267	871-901	1.000000	82.9
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GACTACCATTAAAGCAGCCCA	20	21	50.0	47.6	58.1	58.0	0.0	0.0	1.082797	9459	10267	878-908	1.000000	82.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10955	11619	764-789	1.000000	61.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	10955	11619	758-783	1.000000	60.7
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.4	58.3	0.0	0.0	3.875520	10955	11619	761-786	1.000000	59.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.3	58.3	0.0	0.0	3.958013	10955	11619	765-790	1.000000	59.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	22	20	36.4	50.0	56.2	58.3	0.0	0.0	4.069095	10955	11619	765-790	1.000000	58.2
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.0	56.8	0.0	0.0	2.237066	12066	12996	1023-1073	1.000000	72.5
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.1	56.8	14.8	0.0	2.326325	12066	12996	982-1032	1.000000	72.0
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	12066	12996	1013-1063	1.000000	71.7
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	12066	12996	1018-1068	1.000000	71.7
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.0	57.6	0.0	0.0	2.409970	12066	12996	1013-1063	1.000000	71.1
ycf3-trnS_p1	ycf3-trnS	1	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	21	20	47.6	45.0	58.0	57.8	8.2	0.0	1.233148	44097	44981	635-995	1.000000	81.3
ycf3-trnS_p2	ycf3-trnS	2	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	21	20	47.6	55.0	58.0	57.8	8.2	0.0	1.260963	44097	44981	673-1033	1.000000	80.8
ycf3-trnS_p3	ycf3-trnS	3	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	21	20	47.6	50.0	58.0	58.3	8.2	0.0	1.301418	44097	44981	637-997	1.000000	80.8
ycf3-trnS_p4	ycf3-trnS	4	CATTTATCCGCGATCTAGGCA	AGGTGAAGGTACGGAAAGGA	21	20	47.6	50.0	58.0	58.0	8.2	0.0	1.060292	44097	44981	1010-1045	0.222222	40.6
ycf3-trnS_p5	ycf3-trnS	5	CATTTATCCGCGATCTAGGCA	ACGGAAAGGAAAGAGAGGGA	21	20	47.6	50.0	58.0	58.0	8.2	0.0	1.066120	44097	44981	1000-1035	0.222222	40.6
trnF-ndhJ_p1	trnF-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47918	48563	563-764	1.000000	81.4
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47918	48563	562-763	1.000000	81.3
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47918	48563	570-771	1.000000	80.7
trnF-ndhJ_p4	trnF-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47918	48563	564-765	1.000000	79.2
trnF-ndhJ_p5	trnF-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47918	48563	563-764	1.000000	78.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	ATCATATTCATAAAGCAGAAACA	CCGAGAAAGTCTACGGTTCG	23	20	26.1	55.0	52.2	58.1	0.0	0.0	8.877702	50235	51266	1072-1141	1.000000	51.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCATATTCATAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	23	20	26.1	55.0	52.2	58.1	0.0	0.0	8.877702	50235	51266	1071-1140	1.000000	51.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	ATCATATTCATAAAGCAGAAACAT	CCGAGAAAGTCTACGGTTCG	24	20	25.0	55.0	52.9	58.1	0.0	0.0	9.243544	50235	51266	1072-1141	1.000000	51.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	ATCATATTCATAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	23	20	26.1	55.0	52.2	58.7	0.0	0.0	9.453519	50235	51266	1057-1126	1.000000	51.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCATATTCATAAAGCAGAAACAT	GTTCGAGTCCGTATAGCCCT	23	20	26.1	55.0	52.2	58.7	0.0	0.0	9.453519	50235	51266	1056-1125	1.000000	51.3
trnM-atpE_p1	trnM-atpE	1	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	20	20	50.0	55.0	58.4	57.9	13.2	7.8	0.538239	52168	52379	310-369	1.000000	86.4
trnM-atpE_p2	trnM-atpE	2	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	20	20	50.0	55.0	58.4	58.1	13.2	0.0	0.551943	52168	52379	312-371	1.000000	86.2
trnM-atpE_p3	trnM-atpE	3	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.558762	52168	52379	314-373	1.000000	86.1
trnM-atpE_p4	trnM-atpE	4	TATTGCTTTCATACGGCGGG	GGGTAGAGGCTATCAATGCG	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.497380	52168	52379	285-339	0.972222	85.2
trnM-atpE_p5	trnM-atpE	5	TATTGCTTTCATACGGCGGG	CGGGTAGAGGCTATCAATGC	20	20	50.0	55.0	58.4	57.9	13.2	0.0	0.497380	52168	52379	286-340	0.972222	85.1
accD_p1	accD	1	TGATATGCTTATTCTGATAGGAA	ACAAAGAAAACCCCATTCGTC	23	21	30.4	42.9	52.5	57.0	0.0	0.0	10.464915	56956	58548	1642-1771	0.944444	48.3
accD_p2	accD	2	TTGATATGCTTATTCTGATAGGA	ACAAAGAAAACCCCATTCGTC	23	21	30.4	42.9	52.5	57.0	0.0	0.0	10.464915	56956	58548	1643-1772	0.944444	48.3
accD_p3	accD	3	TGATATGCTTATTCTGATAGGAA	CAACAAAGAAAACCCCATTCGT	23	22	30.4	40.9	52.5	57.9	0.0	0.0	10.516381	56956	58548	1644-1773	0.944444	48.3
accD_p4	accD	4	TTGATATGCTTATTCTGATAGGA	CAACAAAGAAAACCCCATTCGT	23	22	30.4	40.9	52.5	57.9	0.0	0.0	10.516381	56956	58548	1645-1774	0.944444	48.3
accD_p5	accD	5	TGATATGCTTATTCTGATAGGAAA	ACAAAGAAAACCCCATTCGTC	24	21	29.2	42.9	53.4	57.0	0.0	0.0	10.628856	56956	58548	1642-1771	0.944444	48.3
rps18-rpl20_p1	rps18-rpl20	1	AACCGAGTCGACCACTAGAA	AGACAGTTGCTTCTTAATCGT	20	21	50.0	38.1	58.1	55.3	15.6	0.0	3.828133	68193	68440	406-423	1.000000	58.0
rps18-rpl20_p2	rps18-rpl20	2	AAGAACCGAGTCGACCACTA	AGACAGTTGCTTCTTAATCGT	20	21	50.0	38.1	58.1	55.3	15.6	0.0	3.828133	68193	68440	409-426	1.000000	58.0
rps18-rpl20_p3	rps18-rpl20	3	TTGAAAGAACCGAGTCGACC	AGACAGTTGCTTCTTAATCGT	20	21	50.0	38.1	57.9	55.3	15.6	0.0	3.874205	68193	68440	413-430	1.000000	57.5
rps18-rpl20_p4	rps18-rpl20	4	CCGAGTCGACCACTAGAACT	AGACAGTTGCTTCTTAATCGT	20	21	55.0	38.1	58.5	55.3	15.6	0.0	4.278656	68193	68440	404-421	1.000000	54.5
rps18-rpl20_p5	rps18-rpl20	5	AGAACCGAGTCGACCACTAG	AGACAGTTGCTTCTTAATCGT	20	21	55.0	38.1	58.5	55.3	15.6	0.0	4.278656	68193	68440	408-425	1.000000	54.4
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	20	20	50.0	45.0	58.0	57.6	17.5	9.5	0.425347	111348	112397	1238-1344	1.000000	88.6
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	20	20	45.0	45.0	58.0	57.6	17.5	9.5	0.436636	111348	112397	1236-1342	1.000000	88.5
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	20	20	50.0	50.0	58.0	57.6	17.5	0.0	0.397386	111348	112397	1221-1327	0.972222	87.7
ndhF-rpl32_p4	ndhF-rpl32	4	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	111348	112397	1219-1325	0.972222	87.6
ndhF-rpl32_p5	ndhF-rpl32	5	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	111348	112397	1220-1326	0.972222	87.6
rpl32-trnL_p1	rpl32-trnL	1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	112563	113392	939-1063	1.000000	89.0
rpl32-trnL_p2	rpl32-trnL	2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	112563	113392	940-1064	1.000000	89.0
rpl32-trnL_p3	rpl32-trnL	3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	112563	113392	922-1046	1.000000	88.8
rpl32-trnL_p4	rpl32-trnL	4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	112563	113392	923-1047	1.000000	88.8
rpl32-trnL_p5	rpl32-trnL	5	AAGGAAAGGATATTGGGCGG	AGCAAAGCCCTGGGTAAAAA	20	20	50.0	45.0	57.6	57.9	0.0	15.6	0.460576	112563	113392	1443-1568	1.000000	88.3
ycf1_p1	ycf1	1	TCCACGATACATCCCAGTCA	GGCCCTCCTATCTCTTCCTT	20	20	50.0	55.0	57.8	57.9	0.0	0.0	0.263787	127327	127446	1217-1307	1.000000	88.4
ycf1_p2	ycf1	2	TGCATCAGCGGAGAGTTTTT	GGCCCTCCTATCTCTTCCTT	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.214533	127327	127446	1001-1058	0.861111	82.2
ycf1_p3	ycf1	3	TGCATCAGCGGAGAGTTTTT	TCGTTGTGGTCGGACTCTAT	20	20	45.0	50.0	58.1	58.2	0.0	8.2	0.273501	127327	127446	1049-1106	0.861111	81.6
ycf1_p4	ycf1	4	TGCATCAGCGGAGAGTTTTT	ATCAATTCGGTCGTTGTGGT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.282692	127327	127446	1059-1116	0.861111	81.5
ycf1_p5	ycf1	5	TGCATCAGCGGAGAGTTTTT	CTAGCACTGGGTAGACCTCA	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.249177	127327	127446	861-918	0.861111	81.3