primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TCTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAGAAACT	20	21	50.0	47.6	58.3	57.9	0.0	0.0	1.393263	4714	5397	783-789	1.000000	69.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCTAGCCGCACTTAAAAGCC	TTAAAAAGGGCGGACGTTTT	20	20	50.0	40.0	58.3	56.5	0.0	8.4	1.806351	4714	5397	750-756	1.000000	66.5
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTAGCCGCACTTAAAAGCC	TTTAAAAAGGGCGGACGTTT	20	20	50.0	40.0	58.3	56.5	0.0	0.0	1.806351	4714	5397	751-757	1.000000	66.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTAGCCGCACTTAAAAGCC	TTTTAAAAAGGGCGGACGTT	20	20	50.0	40.0	58.3	56.5	0.0	0.0	1.806351	4714	5397	752-758	1.000000	66.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCTAGCCGCACTTAAAAGCC	GGTGCTCAACCTACAGAAAC	20	20	50.0	50.0	58.3	56.4	0.0	0.0	1.903494	4714	5397	783-789	1.000000	65.7
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GGAACCTGTATGCGGTTGAT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	13.0	16.9	0.114488	6599	7207	679-680	1.000000	79.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	GGAACCTGTATGCGGTTGAT	TAAGGCATCGGGTTTTGGTC	20	20	50.0	50.0	58.0	58.2	13.0	0.0	0.209408	6599	7207	709-710	1.000000	78.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6599	7207	745-746	1.000000	78.6
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.2	8.1	0.0	0.310345	6599	7207	775-776	1.000000	77.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTTGGAACCTGTATGCGGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.7	58.1	9.1	16.9	0.356036	6599	7207	682-683	1.000000	77.5
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	13946	14722	864-864	1.000000	78.7
atpH-atpI_p2	atpH-atpI	2	GCCAACTCCAGGTCCAATAG	TTTTGCAACTTTAGCCGCAG	20	20	55.0	45.0	58.0	57.9	0.0	0.0	0.176863	13946	14722	913-913	1.000000	78.7
atpH-atpI_p3	atpH-atpI	3	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	57.9	57.9	1.4	0.0	0.195711	13946	14722	856-856	1.000000	78.5
atpH-atpI_p4	atpH-atpI	4	AGCCAATCCAGCAGCAATAA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.281186	13946	14722	880-880	1.000000	77.8
atpH-atpI_p5	atpH-atpI	5	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCCGCAG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.294628	13946	14722	857-857	1.000000	77.7
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	ACATTCCCTCGTTTCGATCC	GGCGTCGATAAGTGAGTTGT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.318954	26954	28093	1279-1287	1.000000	78.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	GCCCTGATCAATGAACCTCC	GGCGTCGATAAGTGAGTTGT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.322682	26954	28093	1341-1349	1.000000	78.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	ACATTCCCTCGTTTCGATCC	CGGATTTGAACTGGGGAACA	20	20	50.0	50.0	57.7	58.1	0.0	0.0	0.406837	26954	28093	1221-1229	1.000000	77.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GCCCTGATCAATGAACCTCC	CGGATTTGAACTGGGGAACA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.410565	26954	28093	1283-1291	1.000000	77.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CATTCCCTCGTTTCGATCCC	GGCGTCGATAAGTGAGTTGT	20	20	55.0	50.0	58.4	58.0	0.0	0.0	0.424777	26954	28093	1278-1286	1.000000	77.2
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	31739	32576	988-991	1.000000	77.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	31739	32576	991-994	1.000000	77.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	CAAAAACGAAACGGTCCCTC	20	20	55.0	50.0	58.1	57.6	7.6	0.0	0.458613	31739	32576	987-990	1.000000	76.7
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.480767	31739	32576	990-993	1.000000	76.5
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.3	58.2	0.0	0.0	0.528876	31739	32576	965-968	1.000000	76.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	CAAATGCGAGGCTCTAACCT	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	58.0	58.1	13.5	0.0	0.093906	46587	47372	860-865	1.000000	79.8
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCATTACAAATGCGAGGCTC	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	57.9	58.1	3.9	0.0	0.175108	46587	47372	866-871	1.000000	79.2
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GACTCGAACCGATGACCATC	AGCCAATTAAGTCCGTAGCG	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.222618	46587	47372	886-891	1.000000	78.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CAAATGCGAGGCTCTAACCT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	13.5	10.2	0.278287	46587	47372	848-853	1.000000	78.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ACAAATGCGAGGCTCTAACC	AGCCAATTAAGTCCGTAGCG	20	20	50.0	50.0	58.3	58.1	13.5	0.0	0.325001	46587	47372	861-866	1.000000	78.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATCCACGCTTGAAACGGATT	20	20	55.0	45.0	58.0	58.2	10.3	0.0	0.181289	48573	49168	769-770	1.000000	78.8
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATCCACGCTTGAAACGGATT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.230679	48573	49168	733-734	1.000000	78.4
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	ATCCACGCTTGAAACGGATT	20	20	55.0	45.0	57.9	58.2	2.5	0.0	0.315082	48573	49168	757-758	1.000000	77.7
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	20	20	55.0	50.0	58.0	57.7	10.3	5.0	0.350916	48573	49168	746-747	1.000000	77.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGAATGGTCGGGATAGCTCA	ATCCACGCTTGAAACGGATT	20	20	50.0	45.0	58.2	58.2	0.0	0.0	0.390555	48573	49168	778-779	1.000000	77.1
trnV-UAC_p1	trnV-UAC	1	AAGAGCCAACTGATCGGTTC	CGTCCGATCGGGGAATATTT	20	20	50.0	50.0	57.9	57.5	17.2	32.8	0.551214	51731	52423	870-873	1.000000	76.0
trnV-UAC_p2	trnV-UAC	2	AAGAGCCAACTGATCGGTTC	ACGTCCGATCGGGGAATATT	20	20	50.0	50.0	57.9	58.7	17.2	32.8	0.765460	51731	52423	871-874	1.000000	74.3
trnV-UAC_p3	trnV-UAC	3	AAAAGAGCCAACTGATCGGT	CGTCCGATCGGGGAATATTT	20	20	45.0	50.0	57.4	57.5	0.0	32.8	1.019232	51731	52423	872-875	1.000000	72.3
trnV-UAC_p4	trnV-UAC	4	AAAGAGCCAACTGATCGGTT	CGTCCGATCGGGGAATATTT	20	20	45.0	50.0	57.4	57.5	14.4	32.8	1.019232	51731	52423	871-874	1.000000	72.3
trnV-UAC_p5	trnV-UAC	5	AAGAGCCAACTGATCGGTTC	ACGTCCGATCGGGGAATAT	20	19	50.0	52.6	57.9	58.0	17.2	32.8	1.147644	51731	52423	871-874	1.000000	71.3
rbcL_p1	rbcL	1	TGAAGTTGTAGGGAGGGACTT	AGTAAAAGATTGGGCCGAGT	21	20	47.6	45.0	58.0	56.8	0.0	0.0	2.187963	55586	57013	1512-1512	1.000000	62.8
rbcL_p2	rbcL	2	TGAAGTTGTAGGGAGGGACTT	AGTAAAAGATTGGGCCGAGTT	21	21	47.6	42.9	58.0	57.6	0.0	0.0	2.448890	55586	57013	1512-1512	1.000000	60.7
rbcL_p3	rbcL	3	TTGAAGTTGTAGGGAGGGAC	AGTAAAAGATTGGGCCGAGT	20	20	50.0	45.0	56.5	56.8	0.0	0.0	2.713295	55586	57013	1513-1513	1.000000	58.6
rbcL_p4	rbcL	4	TTGAAGTTGTAGGGAGGGAC	AGTAAAAGATTGGGCCGAGTT	20	21	50.0	42.9	56.5	57.6	0.0	0.0	2.974221	55586	57013	1513-1513	1.000000	56.5
rbcL_p5	rbcL	5	TGAAGTTGTAGGGAGGGACTT	GTAAAAGATTGGGCCGAGTT	21	20	47.6	45.0	58.0	56.0	0.0	0.0	2.988996	55586	57013	1511-1511	1.000000	56.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTTAAAGCT	20	22	50.0	40.9	57.9	57.0	6.4	0.0	3.074685	65004	66214	1375-1377	1.000000	55.5
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	CGGAGGTTATAGTTAAAGCTGC	20	22	50.0	45.5	57.9	56.9	6.4	0.0	3.107411	65004	66214	1373-1375	1.000000	55.3
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	GCGGAGGTTATAGTTAAAGCTG	20	22	50.0	45.5	57.9	56.9	6.4	0.0	3.107411	65004	66214	1374-1376	1.000000	55.3
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTTATAGTTAAAGCT	20	22	50.0	40.9	57.8	57.0	24.9	0.0	3.179693	65004	66214	1298-1300	1.000000	54.7
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AGCGGAGGTTATAGTTAAAGCT	20	22	45.0	40.9	58.2	57.0	0.0	0.0	3.194371	65004	66214	1384-1386	1.000000	54.6
psaJ-rpl33_p1	psaJ-rpl33	1	GATGCGTTGACATTCCCTTT	CCACTCTTTCGAACACAAGC	20	20	45.0	50.0	56.7	57.3	0.0	0.0	1.973878	67564	67988	521-526	1.000000	65.3
psaJ-rpl33_p2	psaJ-rpl33	2	TGCGTTGACATTCCCTTTTT	CCACTCTTTCGAACACAAGC	20	20	40.0	50.0	56.4	57.3	0.0	0.0	2.227772	67564	67988	519-524	1.000000	63.3
psaJ-rpl33_p3	psaJ-rpl33	3	GATGCGTTGACATTCCCTTTT	CCACTCTTTCGAACACAAGC	21	20	42.9	50.0	57.4	57.3	0.0	0.0	2.246367	67564	67988	521-526	1.000000	63.1
psaJ-rpl33_p4	psaJ-rpl33	4	GATGCGTTGACATTCCCTTT	ACTCTTTCGAACACAAGCAGT	20	21	45.0	42.9	56.7	58.1	0.0	0.0	2.397123	67564	67988	519-524	1.000000	61.9
psaJ-rpl33_p5	psaJ-rpl33	5	ATGCGTTGACATTCCCTTTT	CCACTCTTTCGAACACAAGC	20	20	40.0	50.0	56.2	57.3	0.0	0.0	2.458743	67564	67988	520-525	1.000000	61.4
rps19_p1	rps19	1	TCGTGCTTTGTCAACAGACA	TGGGTAGAGCCGGATCTAAG	20	20	45.0	55.0	58.0	58.0	0.0	29.9	0.020340	84625	84903	776-776	1.000000	80.2
rps19_p2	rps19	2	TCGTGCTTTGTCAACAGACA	ATACACTATGGGGATGGCGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.041314	84625	84903	1110-1110	1.000000	80.0
rps19_p3	rps19	3	TCGTGCTTTGTCAACAGACA	GGGCCCCAATTGGTAGAAAA	20	20	45.0	50.0	58.0	58.1	0.0	22.9	0.071558	84625	84903	677-677	1.000000	79.9
rps19_p4	rps19	4	CCACACAATAATTCGCGTCG	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	57.9	58.0	5.8	29.9	0.074806	84625	84903	1159-1159	1.000000	79.7
rps19_p5	rps19	5	CGACCACACAATAATTCGCG	TGGGTAGAGCCGGATCTAAG	20	20	50.0	55.0	57.9	58.0	0.0	29.9	0.074806	84625	84903	1162-1162	1.000000	79.7
rpl2-trnI-CAU_p1	rpl2-trnI-CAU	1	CAACGACGCTGTCCATAGAT	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	57.8	58.0	0.0	2.3	0.219039	85795	86603	981-995	1.000000	79.3
rpl2-trnI-CAU_p2	rpl2-trnI-CAU	2	CGTGTGCTCGAGTTAGAAGT	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	57.7	58.0	15.1	2.3	0.342590	85795	86603	912-926	1.000000	78.4
rpl2-trnI-CAU_p3	rpl2-trnI-CAU	3	CGACGCTGTCCATAGATCAA	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	57.5	58.0	0.0	2.3	0.501220	85795	86603	978-992	1.000000	77.0
rpl2-trnI-CAU_p4	rpl2-trnI-CAU	4	AACGACGCTGTCCATAGATC	GAATGGTTAAAGCGCCCAAC	20	20	50.0	50.0	57.5	58.0	0.0	2.3	0.502914	85795	86603	980-994	1.000000	77.0
rpl2-trnI-CAU_p5	rpl2-trnI-CAU	5	CAACGACGCTGTCCATAGAT	GCTAAGCATCCATGGCTGAA	20	20	50.0	50.0	57.8	58.3	0.0	19.8	0.525895	85795	86603	998-1012	1.000000	76.8
ycf2_p1	ycf2	1	CAGAACCGAATTCCTTCTGGT	CGTTAAAGTGAAGAACTGAACCA	21	23	47.6	39.1	57.9	57.3	22.2	0.0	4.787703	89988	90107	269-269	1.000000	42.6
ycf2_p2	ycf2	2	CAGAACCGAATTCCTTCTGGT	AGTGAAGAACTGAACCAAGA	21	20	47.6	40.0	57.9	54.1	22.2	0.0	4.928996	89988	90107	263-263	1.000000	41.5
ycf2_p3	ycf2	3	CAGAACCGAATTCCTTCTGGT	TCTCTTTCTTCCTCATCAATCGA	21	23	47.6	39.1	57.9	57.1	22.2	0.0	4.981314	89988	90107	241-241	1.000000	41.1
ycf2_p4	ycf2	4	CAGAACCGAATTCCTTCTGGT	TCGTTAAAGTGAAGAACTGAACC	21	23	47.6	39.1	57.9	57.0	22.2	0.0	5.038285	89988	90107	270-270	1.000000	40.9
ycf2_p5	ycf2	5	CAGAACCGAATTCCTTCTGGT	AGTGAAGAACTGAACCAAGAA	21	21	47.6	38.1	57.9	55.0	22.2	0.0	5.061738	89988	90107	263-263	1.000000	40.9
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	AGGATTCGAACCTACGACCA	ATAGAGTCCGACCACAACGA	20	20	50.0	50.0	58.2	58.2	15.0	0.0	0.326353	107302	107829	628-674	1.000000	81.6
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	AATCAGTTAACAGCCGACCG	ATAGAGTCCGACCACAACGA	20	20	50.0	50.0	58.3	58.2	8.6	0.0	0.447710	107302	107829	609-655	1.000000	80.7
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	CCCCAAGTAGGATTCGAACC	ATAGAGTCCGACCACAACGA	20	20	55.0	50.0	57.7	58.2	0.0	0.0	0.496921	107302	107829	636-682	1.000000	80.3
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	CGAGCCCCTTCATTCTGAAT	ATAGAGTCCGACCACAACGA	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.508298	107302	107829	682-728	1.000000	80.2
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	AGGATTCGAACCTACGACCA	AATAGAGTCCGACCACAACG	20	20	50.0	50.0	58.2	57.4	15.0	0.0	0.805963	107302	107829	629-675	1.000000	77.8
ycf1_p1	ycf1	1	ACCCGTGGTATCATCGGTAT	GGACCCTTTCGAACAAGAGG	20	20	50.0	55.0	58.0	58.2	13.6	0.0	0.195686	126978	127097	394-484	1.000000	84.3
ycf1_p2	ycf1	2	TATCAAAGTCACTTCGCCCG	GGACCCTTTCGAACAAGAGG	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.194899	126978	127097	427-517	1.000000	84.1
ycf1_p3	ycf1	3	ACCCGTGGTATCATCGGTAT	ACTGGACCCTTTCGAACAAG	20	20	50.0	50.0	58.0	57.7	13.6	0.0	0.261487	126978	127097	397-487	1.000000	83.7
ycf1_p4	ycf1	4	TATCAAAGTCACTTCGCCCG	ACTGGACCCTTTCGAACAAG	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.260700	126978	127097	430-520	1.000000	83.6
ycf1_p5	ycf1	5	AAATCTTCGAACCACTCCGG	GGACCCTTTCGAACAAGAGG	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.285078	126978	127097	891-981	1.000000	82.6