primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rpoC2_p1 rpoC2 1 AAGCATTGCACACGTTAACG GCTGGGTACTTGACTCGAAG 20 20 45.0 55.0 58.0 58.0 25.7 0.0 0.033368 5626 5745 1519-1519 0.500000 59.7 rpoC2_p2 rpoC2 2 AAGCATTGCACACGTTAACG AGGGGCTCAAATGCTTCAAA 20 20 45.0 45.0 58.0 58.0 25.7 0.0 0.045046 5626 5745 907-907 0.500000 59.6 rpoC2_p3 rpoC2 3 AAGCATTGCACACGTTAACG ATTGGAGAACCTGGGACTCA 20 20 45.0 50.0 58.0 58.0 25.7 18.8 0.075829 5626 5745 1129-1129 0.500000 59.4 rpoC2_p4 rpoC2 4 AAGCATTGCACACGTTAACG TGGAGAACCTGGGACTCAAT 20 20 45.0 50.0 58.0 58.0 25.7 18.8 0.075829 5626 5745 1127-1127 0.500000 59.4 rpoC2_p5 rpoC2 5 AAGCATTGCACACGTTAACG TATGGGTGAAGTCTGGCTGA 20 20 45.0 50.0 58.0 58.0 25.7 0.0 0.079827 5626 5745 1201-1201 0.500000 59.4 psbF-rpoBa_p1 psbF-rpoBa 1 GGCTGGCATATCGTTAACCA AGCTCCCCCGTTCAGTATAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.208219 15185 17258 2908-2908 0.500000 58.3 psbF-rpoBa_p2 psbF-rpoBa 2 TGGCTGGCATATCGTTAACC AGCTCCCCCGTTCAGTATAA 20 20 50.0 50.0 58.0 57.8 0.0 0.0 0.208219 15185 17258 2909-2909 0.500000 58.3 psbF-rpoBa_p3 psbF-rpoBa 3 GGAGTTTTTCTTTGGCTGGC AGCTCCCCCGTTCAGTATAA 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.324866 15185 17258 2921-2921 0.500000 57.4 psbF-rpoBa_p4 psbF-rpoBa 4 GGCTGGCATATCGTTAACCA CTAGCTCCCCCGTTCAGTAT 20 20 50.0 55.0 58.0 58.3 0.0 0.0 0.332334 15185 17258 2910-2910 0.500000 57.3 psbF-rpoBa_p5 psbF-rpoBa 5 TGGCTGGCATATCGTTAACC CTAGCTCCCCCGTTCAGTAT 20 20 50.0 55.0 58.0 58.3 0.0 0.0 0.332334 15185 17258 2911-2911 0.500000 57.3 trnY(gua)-psbK_p1 trnY(gua)-psbK 1 CTCGTGAAGGTGGCGAAATA CACCCCCACCGTTTAGTTTT 20 20 50.0 50.0 58.0 58.3 0.0 0.0 0.309739 47162 47957 2091-2091 0.500000 57.5 trnY(gua)-psbK_p2 trnY(gua)-psbK 2 TACGTTGGTTCGAATCCGAC CACCCCCACCGTTTAGTTTT 20 20 50.0 50.0 58.0 58.3 19.3 0.0 0.323902 47162 47957 1397-1397 0.500000 57.4 trnY(gua)-psbK_p3 trnY(gua)-psbK 3 AGTCCTGAATTAGCTGCTGC CACCCCCACCGTTTAGTTTT 20 20 50.0 50.0 58.0 58.3 13.4 0.0 0.340686 47162 47957 2050-2050 0.500000 57.3 trnY(gua)-psbK_p4 trnY(gua)-psbK 4 TTTGCCTACGTTGGTTCGAA CACCCCCACCGTTTAGTTTT 20 20 45.0 50.0 58.0 58.3 17.4 0.0 0.354328 47162 47957 1403-1403 0.500000 57.2 trnY(gua)-psbK_p5 trnY(gua)-psbK 5 TTCAGAACACCCCCTGGTAT CACCCCCACCGTTTAGTTTT 20 20 50.0 50.0 57.9 58.3 0.0 0.0 0.358903 47162 47957 1721-1721 0.500000 57.1 chlL_p1 chlL 1 TGGCTTCTGCGAAAAAGCTA GGGCCTATCTGTTACACGTG 20 20 45.0 55.0 58.1 58.1 0.5 15.5 0.173191 63273 64163 1782-1782 0.500000 58.6 chlL_p2 chlL 2 TGGCTTCTGCGAAAAAGCTA GCACAGGCCAAAAGGTAAAC 20 20 45.0 50.0 58.1 57.9 0.5 5.0 0.258039 63273 64163 1858-1858 0.500000 57.9 chlL_p3 chlL 3 AAAAGGCAAAGGCTAGCGTA GGGCCTATCTGTTACACGTG 20 20 45.0 55.0 57.8 58.1 10.9 15.5 0.258269 63273 64163 1854-1854 0.500000 57.9 chlL_p4 chlL 4 AAAGGCAAAGGCTAGCGTAA GGGCCTATCTGTTACACGTG 20 20 45.0 55.0 57.8 58.1 10.9 15.5 0.258269 63273 64163 1853-1853 0.500000 57.9 chlL_p5 chlL 5 AAGGCAAAGGCTAGCGTAAA GGGCCTATCTGTTACACGTG 20 20 45.0 55.0 57.8 58.1 10.9 15.5 0.258269 63273 64163 1852-1852 0.500000 57.9 trnW(cca)-atpA_p1 trnW(cca)-atpA 1 AGGTGGGTTTTTAACGTCCC GTAGCTCCAGTGTAAGGTGC 20 20 50.0 55.0 58.0 58.0 0.8 0.0 0.026067 79772 80492 1885-1885 0.500000 59.8 trnW(cca)-atpA_p2 trnW(cca)-atpA 2 AGGTGGGTTTTTAACGTCCC ATCAACTGGGCGAGCTAAAG 20 20 50.0 50.0 58.0 58.0 0.8 0.0 0.044365 79772 80492 1502-1502 0.500000 59.6 trnW(cca)-atpA_p3 trnW(cca)-atpA 3 AGGTGGGTTTTTAACGTCCC ATACAGAACGACGCGAAACA 20 20 50.0 45.0 58.0 57.9 0.8 15.9 0.069214 79772 80492 1581-1581 0.500000 59.4 trnW(cca)-atpA_p4 trnW(cca)-atpA 4 TTATCATTCCCGCGACACAG GTAGCTCCAGTGTAAGGTGC 20 20 50.0 55.0 58.1 58.0 8.2 0.0 0.076951 79772 80492 2151-2151 0.500000 59.4 trnW(cca)-atpA_p5 trnW(cca)-atpA 5 GTCGTGGGTTCAAGTCCTAC GTAGCTCCAGTGTAAGGTGC 20 20 55.0 55.0 57.9 58.0 0.0 0.0 0.087519 79772 80492 1483-1483 0.500000 59.3 cemA_p1 cemA 1 AAGGCCAAAAGGTGAACGAT TTCATGCGCTATCAACTGCT 20 20 45.0 45.0 58.0 58.0 5.0 9.7 0.033044 83553 84764 2482-2482 0.500000 59.7 cemA_p2 cemA 2 AGGCCAAAAGGTGAACGATT TTCATGCGCTATCAACTGCT 20 20 45.0 45.0 58.0 58.0 5.0 9.7 0.033044 83553 84764 2481-2481 0.500000 59.7 cemA_p3 cemA 3 AATGACCCAGCACGTAATCC TTCATGCGCTATCAACTGCT 20 20 50.0 45.0 58.0 58.0 0.0 9.7 0.066807 83553 84764 2299-2299 0.500000 59.5 cemA_p4 cemA 4 AAGGCCAAAAGGTGAACGAT TTTTTCTCCCCAATCAGCCC 20 20 45.0 50.0 58.0 58.1 5.0 0.0 0.067433 83553 84764 2062-2062 0.500000 59.5 cemA_p5 cemA 5 AGGCCAAAAGGTGAACGATT TTTTTCTCCCCAATCAGCCC 20 20 45.0 50.0 58.0 58.1 5.0 0.0 0.067433 83553 84764 2061-2061 0.500000 59.5 orf333_p1 orf333 1 CAGCACACCATCACTTAGCT AATTGCGGTGCATGGAAATG 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.117943 121551 122552 2157-2157 0.500000 59.1 orf333_p2 orf333 2 ACATCATGGCATGCTCAACT AATTGCGGTGCATGGAAATG 20 20 45.0 45.0 57.9 58.0 22.6 0.0 0.152260 121551 122552 1999-1999 0.500000 58.8 orf333_p3 orf333 3 GCAATGTGCTCACGGTTTTT AATTGCGGTGCATGGAAATG 20 20 45.0 45.0 58.1 58.0 0.0 0.0 0.161278 121551 122552 1744-1744 0.500000 58.7 orf333_p4 orf333 4 CAGCACACCATCACTTAGCT ACAAAAACCACCGATCCACA 20 20 50.0 45.0 57.9 57.9 0.0 0.0 0.165056 121551 122552 1870-1870 0.500000 58.7 orf333_p5 orf333 5 ACAACATCATGGCATGCTCA AATTGCGGTGCATGGAAATG 20 20 45.0 45.0 58.2 58.0 22.6 0.0 0.170885 121551 122552 2002-2002 0.500000 58.6 orf333-orf579_p1 orf333-orf579 1 AATTTCAGCTCACGGAGACC CAAGTGCACTTCTTCCTGGT 20 20 50.0 50.0 57.9 58.0 5.2 29.0 0.127733 122553 123902 2364-2364 0.500000 59.0 orf333-orf579_p2 orf333-orf579 2 AATTTCAGCTCACGGAGACC TTTAGCGCCGGAATGATTGA 20 20 50.0 45.0 57.9 58.0 5.2 5.0 0.131863 122553 123902 2057-2057 0.500000 58.9 orf333-orf579_p3 orf333-orf579 3 AATTTCAGCTCACGGAGACC TGCCTCCCATTCAGGTTCTA 20 20 50.0 50.0 57.9 58.0 5.2 0.0 0.135190 122553 123902 2315-2315 0.500000 58.9 orf333-orf579_p4 orf333-orf579 4 AATTTCAGCTCACGGAGACC CAGTTGCGAGCGTCATTTTT 20 20 50.0 45.0 57.9 58.0 5.2 0.0 0.137161 122553 123902 2163-2163 0.500000 58.9 orf333-orf579_p5 orf333-orf579 5 AATTTCAGCTCACGGAGACC AGTTTAGCGCCGGAATGATT 20 20 50.0 45.0 57.9 58.0 5.2 5.0 0.138992 122553 123902 2059-2059 0.500000 58.9 orf579-trnG(gcc)_p1 orf579-trnG(gcc) 1 AGACCAAACCCCATTCAACC TAAACCGTGTTGACCTGGTG 20 20 50.0 50.0 58.0 58.0 0.0 2.8 0.053385 125643 127068 2415-2415 0.500000 59.6 orf579-trnG(gcc)_p2 orf579-trnG(gcc) 2 TCGACAAAATCAACGTTGCG TAAACCGTGTTGACCTGGTG 20 20 45.0 50.0 58.0 58.0 23.5 2.8 0.055635 125643 127068 2325-2325 0.500000 59.6 orf579-trnG(gcc)_p3 orf579-trnG(gcc) 3 CGAAGTGGCAAATTGCGAAT TAAACCGTGTTGACCTGGTG 20 20 45.0 50.0 58.0 58.0 12.2 2.8 0.062450 125643 127068 2603-2603 0.500000 59.5 orf579-trnG(gcc)_p4 orf579-trnG(gcc) 4 AGACCAAACCCCATTCAACC AAAGTTTGCTACACGACCGT 20 20 50.0 45.0 58.0 58.1 0.0 0.0 0.065650 125643 127068 1806-1806 0.500000 59.5 orf579-trnG(gcc)_p5 orf579-trnG(gcc) 5 TCGACAAAATCAACGTTGCG AAAGTTTGCTACACGACCGT 20 20 45.0 45.0 58.0 58.1 23.5 0.0 0.067900 125643 127068 1716-1716 0.500000 59.5 rps4-trnS(gga)_p1 rps4-trnS(gga) 1 CACCAGGTCAACACGGTTTA CCGTACCTTAAACCACTCGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.044132 128378 128974 1244-1244 0.500000 59.6 rps4-trnS(gga)_p2 rps4-trnS(gga) 2 CACCAGGTCAACACGGTTTA TATCCACTTATCGCCCTCGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.052582 128378 128974 1780-1780 0.500000 59.6 rps4-trnS(gga)_p3 rps4-trnS(gga) 3 CACCAGGTCAACACGGTTTA GCAGCTTCTTTGGCTTTAGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.055640 128378 128974 1952-1952 0.500000 59.6 rps4-trnS(gga)_p4 rps4-trnS(gga) 4 CACCAGGTCAACACGGTTTA TCCATCAACCAGAAGGAGGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.088496 128378 128974 1882-1882 0.500000 59.3 rps4-trnS(gga)_p5 rps4-trnS(gga) 5 CACCAGGTCAACACGGTTTA GCTTTAGCTGGCGGAACTAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.088776 128378 128974 1940-1940 0.500000 59.3 clpP_p1 clpP 1 CCGAGTGGTTTAAGGTACGG GCATGACCTCTTAACGTCGT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.008260 129305 130885 2110-2110 0.500000 59.9 clpP_p2 clpP 2 ATGCTTGACCTCCACACAAG GCATGACCTCTTAACGTCGT 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.106239 129305 130885 2479-2479 0.500000 59.2 clpP_p3 clpP 3 AAAGGAAAGATGGCCGAGTG GCATGACCTCTTAACGTCGT 20 20 50.0 50.0 58.2 58.0 0.0 0.0 0.181445 129305 130885 2123-2123 0.500000 58.5 clpP_p4 clpP 4 TAATACCCCCTGCTCACCTC GCATGACCTCTTAACGTCGT 20 20 55.0 50.0 58.2 58.0 0.0 0.0 0.205277 129305 130885 1947-1947 0.500000 58.4 clpP_p5 clpP 5 CCGAGTGGTTTAAGGTACGG CCCCGGGGGTATAAAAACAC 20 20 55.0 55.0 58.0 58.2 0.0 34.0 0.233936 129305 130885 2159-2159 0.500000 58.1 trnE(uuc)-trnF(gaa)_p1 trnE(uuc)-trnF(gaa) 1 ATTCGAATCCCCGTTTCCTC CGGGATAGCTCAGTGGTAGA 20 20 50.0 55.0 57.7 58.0 4.4 0.0 0.344540 146085 146553 599-719 1.000000 89.2 trnE(uuc)-trnF(gaa)_p2 trnE(uuc)-trnF(gaa) 2 ATTCGAATCCCCGTTTCCTC CTCAGTGGTAGAGCAGAGGA 20 20 50.0 55.0 57.7 57.9 4.4 0.0 0.463498 146085 146553 591-711 1.000000 88.3 trnE(uuc)-trnF(gaa)_p3 trnE(uuc)-trnF(gaa) 3 ATTCGAATCCCCGTTTCCTC AGTGGTAGAGCAGAGGACTG 20 20 50.0 55.0 57.7 58.2 4.4 0.0 0.481193 146085 146553 588-708 1.000000 88.2 trnE(uuc)-trnF(gaa)_p4 trnE(uuc)-trnF(gaa) 4 ATTCGAATCCCCGTTTCCTC CAGTGGTAGAGCAGAGGACT 20 20 50.0 55.0 57.7 58.2 4.4 0.0 0.481193 146085 146553 589-709 1.000000 88.2 trnE(uuc)-trnF(gaa)_p5 trnE(uuc)-trnF(gaa) 5 ATTCGAATCCCCGTTTCCTC GTGGTAGAGCAGAGGACTGA 20 20 50.0 55.0 57.7 58.2 4.4 0.0 0.486463 146085 146553 587-707 1.000000 88.1 trnL(caa)-ycf1_p1 trnL(caa)-ycf1 1 AAAGCGTCGACTTTTGAGGT GGGCTATTGACGTAGGCAAA 20 20 45.0 50.0 58.1 58.0 14.7 11.2 0.083215 185089 186603 1964-1964 0.500000 59.3 trnL(caa)-ycf1_p2 trnL(caa)-ycf1 2 AAACTGGTAGCGCGGTTAAT GGGCTATTGACGTAGGCAAA 20 20 45.0 50.0 57.9 58.0 19.1 11.2 0.136218 185089 186603 2593-2593 0.500000 58.9 trnL(caa)-ycf1_p3 trnL(caa)-ycf1 3 AAAGCGTCGACTTTTGAGGT CCGCTTCTGTTCCTTGAGTT 20 20 45.0 50.0 58.1 58.1 14.7 0.0 0.166299 185089 186603 2009-2009 0.500000 58.7 trnL(caa)-ycf1_p4 trnL(caa)-ycf1 4 CTGGTAGCGCGGTTAATGAT GGGCTATTGACGTAGGCAAA 20 20 50.0 50.0 58.1 58.0 19.1 11.2 0.166816 185089 186603 2590-2590 0.500000 58.7 trnL(caa)-ycf1_p5 trnL(caa)-ycf1 5 AAACTGGTAGCGCGGTTAAT CCGCTTCTGTTCCTTGAGTT 20 20 45.0 50.0 57.9 58.1 19.1 0.0 0.219302 185089 186603 2638-2638 0.500000 58.2 rrs_p1 rrs 1 AGTTCACAATGGCGTTCACT CTCCGGATCGTTGCTTGTTA 20 20 45.0 50.0 58.0 58.0 0.0 3.6 0.037264 197503 198623 2429-2429 0.500000 59.7 rrs_p2 rrs 2 AGCGCCTGATATTGCAGAAA CTCCGGATCGTTGCTTGTTA 20 20 45.0 50.0 58.0 58.0 2.3 3.6 0.047269 197503 198623 1836-1836 0.500000 59.6 rrs_p3 rrs 3 CCAAAAAGTCCCGGGATGAA CTCCGGATCGTTGCTTGTTA 20 20 50.0 50.0 58.1 58.0 29.7 3.6 0.086711 197503 198623 2060-2060 0.500000 59.3 rrs_p4 rrs 4 AGTTCACAATGGCGTTCACT GAATCTCCGGATCGTTGCTT 20 20 45.0 50.0 58.0 58.1 0.0 3.6 0.091356 197503 198623 2433-2433 0.500000 59.3 rrs_p5 rrs 5 AGCGCCTGATATTGCAGAAA GAATCTCCGGATCGTTGCTT 20 20 45.0 50.0 58.0 58.1 2.3 3.6 0.101361 197503 198623 1840-1840 0.500000 59.2 rrl_p1 rrl 1 CATCGGCTCAAGCTTTCTCT TGGGGAAGATCAGCCTGTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.055941 203982 204101 764-764 0.500000 59.6 rrl_p2 rrl 2 CACCCTGTTTCAATCAACGC TGGGGAAGATCAGCCTGTTA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.085063 203982 204101 694-694 0.500000 59.3 rrl_p3 rrl 3 CGAGTAAGTTTCGACCTGCA TGGGGAAGATCAGCCTGTTA 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.094912 203982 204101 523-523 0.500000 59.2 rrl_p4 rrl 4 CATCGGCTCAAGCTTTCTCT CCTTGACTCTCCCCGTATCT 20 20 50.0 55.0 58.0 57.9 0.0 0.0 0.096084 203982 204101 1367-1367 0.500000 59.2 rrl_p5 rrl 5 AGGTGGGCAGTTTATTTGGG TGGGGAAGATCAGCCTGTTA 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.097395 203982 204101 236-236 0.500000 59.2