primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	145	498	807-1039	1.000000	88.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	145	498	821-1053	1.000000	88.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	145	498	806-1038	1.000000	88.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	145	498	820-1052	1.000000	88.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	145	498	822-1054	1.000000	88.5
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	GAATATGAACCGAGGGATCCC	20	21	50.0	52.4	58.0	57.7	0.0	24.6	1.340720	7552	8890	1172-1465	1.000000	77.8
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	CAATCCGACGCTTTAGTCCA	GGAATATGAACCGAGGGATCC	20	21	50.0	52.4	58.0	57.7	0.0	1.9	1.340720	7552	8890	1173-1466	1.000000	77.8
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	AATCCGACGCTTTAGTCCAC	GAATATGAACCGAGGGATCCC	20	21	50.0	52.4	58.0	57.7	0.0	24.6	1.342108	7552	8890	1171-1464	1.000000	77.8
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AATCCGACGCTTTAGTCCAC	GGAATATGAACCGAGGGATCC	20	21	50.0	52.4	58.0	57.7	0.0	1.9	1.342108	7552	8890	1172-1465	1.000000	77.8
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AAATTCCAAGTCATCGCCGA	GAATATGAACCGAGGGATCCC	20	21	45.0	52.4	57.9	57.7	0.0	24.6	1.444088	7552	8890	1248-1541	1.000000	76.7
trnG-GCC-trnR-UCU_p1	trnG-GCC-trnR-UCU	1	TAGCCTTCCAAGCTAGCGAT	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.6	56.9	9.5	0.0	2.637446	9676	10486	617-892	1.000000	68.6
trnG-GCC-trnR-UCU_p2	trnG-GCC-trnR-UCU	2	TAGCCTTCCAAGCTAGCGAT	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.6	57.9	9.5	0.0	2.720774	9676	10486	623-898	1.000000	67.9
trnG-GCC-trnR-UCU_p3	trnG-GCC-trnR-UCU	3	CGTCGACTATAACCCCTAGC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.0	56.9	14.7	0.0	3.037011	9676	10486	633-908	1.000000	65.3
trnG-GCC-trnR-UCU_p4	trnG-GCC-trnR-UCU	4	CCCCTAGCCTTCCAAGCTA	AGAAGACCTCTGTCCTATCCA	19	21	57.9	47.6	58.1	56.9	0.0	0.0	3.120670	9676	10486	621-896	1.000000	64.7
trnG-GCC-trnR-UCU_p5	trnG-GCC-trnR-UCU	5	CGTCGACTATAACCCCTAGC	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	57.0	57.9	14.7	0.0	3.120339	9676	10486	639-914	1.000000	64.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	TCACCGAAGAAGCAGAAAGC	21	20	47.6	50.0	57.3	58.5	0.0	0.0	2.179865	10559	11146	450-690	1.000000	68.9
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	AGGAACTGAAGGAGCGTTTT	21	20	47.6	45.0	57.3	57.4	0.0	0.0	2.333107	10559	11146	411-651	1.000000	67.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	ACATTCACCGAAGAAGCAGA	21	20	47.6	45.0	57.3	57.2	0.0	0.0	2.532277	10559	11146	454-694	1.000000	66.1
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	TGGATAGGACAGAGGTCTTCT	TCACCGAAGAAGCAGAAAGC	21	20	47.6	50.0	56.9	58.5	0.0	0.0	2.538678	10559	11146	485-725	1.000000	65.9
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGTTCAAATCCTATTGGACGC	GGAACTGAAGGAGCGTTTTC	21	20	47.6	50.0	57.3	57.0	0.0	0.0	2.664221	10559	11146	410-650	1.000000	65.3
atpA-atpF_p1	atpA-atpF	1	CCGTCGCCTACTTGAAGTAC	CAACGGGTTTTCCAACAAGC	20	20	55.0	50.0	58.1	58.4	0.0	0.0	0.528147	12671	12728	261-302	1.000000	76.9
atpA-atpF_p2	atpA-atpF	2	TACTAATCTCGTCGGCTCGA	CAACGGGTTTTCCAACAAGC	20	20	50.0	50.0	57.8	58.4	0.0	0.0	0.663271	12671	12728	179-220	1.000000	76.3
atpA-atpF_p3	atpA-atpF	3	TTGTTCAATACGTTCGCGGA	CAACGGGTTTTCCAACAAGC	20	20	45.0	50.0	58.2	58.4	6.1	0.0	0.652790	12671	12728	205-246	1.000000	76.2
atpA-atpF_p4	atpA-atpF	4	CGTCGGCTCGAATAGTTACC	CAACGGGTTTTCCAACAAGC	20	20	55.0	50.0	58.2	58.4	0.0	0.0	0.682045	12671	12728	170-211	1.000000	76.2
atpA-atpF_p5	atpA-atpF	5	ATCTCGTCGGCTCGAATAGT	CAACGGGTTTTCCAACAAGC	20	20	50.0	50.0	58.4	58.4	0.0	0.0	0.838130	12671	12728	174-215	1.000000	74.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GCGGTAGGAACTAGAATGAACA	TCCCCGGGATTGTAGTTCAA	22	20	45.5	50.0	57.9	58.3	0.0	26.3	2.460420	32251	33596	1483-1594	1.000000	64.2
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GCGGTAGGAACTAGAATGAACA	TTCCCCGGGATTGTAGTTCA	22	20	45.5	50.0	57.9	58.3	0.0	26.3	2.460420	32251	33596	1484-1595	1.000000	64.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	20	20	45.0	50.0	55.8	58.3	0.0	26.3	2.570277	32251	33596	1471-1582	1.000000	63.3
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAACAGTGCAGTAGC	TTCCCCGGGATTGTAGTTCA	20	20	45.0	50.0	55.8	58.3	0.0	26.3	2.570277	32251	33596	1472-1583	1.000000	63.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GCGGTAGGAACTAGAATGAACA	TTCAATTGGTCAGAGCACCG	22	20	45.5	50.0	57.9	58.5	0.0	5.4	2.596774	32251	33596	1468-1579	1.000000	63.1
trnE-UUC-trnT_GGU_p1	trnE-UUC-trnT_GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	34339	35479	1217-1244	1.000000	83.3
trnE-UUC-trnT_GGU_p2	trnE-UUC-trnT_GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	34339	35479	1196-1223	1.000000	83.2
trnE-UUC-trnT_GGU_p3	trnE-UUC-trnT_GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	34339	35479	1196-1223	1.000000	82.8
trnE-UUC-trnT_GGU_p4	trnE-UUC-trnT_GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	34339	35479	1175-1202	1.000000	82.7
trnE-UUC-trnT_GGU_p5	trnE-UUC-trnT_GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	34339	35479	1193-1220	1.000000	82.7
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.1	57.8	0.0	1.1	1.036941	40522	41370	1000-1310	1.000000	78.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	40522	41370	1003-1313	1.000000	75.7
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	56.8	57.8	0.0	1.1	1.328499	40522	41370	1001-1311	1.000000	75.7
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TCTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	58.4	57.8	0.0	1.1	1.549901	40522	41370	1004-1314	1.000000	73.9
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	21	20	47.6	50.0	57.6	57.8	0.0	1.1	1.593372	40522	41370	1002-1312	1.000000	73.5
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTTGCTAATCGGGGATA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	56.8	57.8	0.0	0.0	1.441190	55406	56380	939-1119	1.000000	75.4
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTCCGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	19	20	57.9	50.0	58.3	57.8	0.0	0.0	1.489381	55406	56380	945-1125	1.000000	75.0
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTCTCCGGCCCTTTGCTAAT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	59.4	57.8	0.0	0.0	1.573560	55406	56380	947-1127	1.000000	74.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTTGCTAATCGGGGATAA	AGAAGGTCTACGGTTCGAGT	21	20	47.6	50.0	57.5	57.8	0.0	0.0	1.699406	55406	56380	939-1119	1.000000	73.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTTGCTAATCGGGGATA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	56.8	57.5	0.0	0.0	1.784962	55406	56380	938-1118	1.000000	72.7
rps12-clpP_p1	rps12-clpP	1	GCGGGGGATTTAGTGACATT	TATGTCAGCAACAGAAGCCC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.166643	76089	76290	328-368	1.000000	80.5
rps12-clpP_p2	rps12-clpP	2	GCGGGGGATTTAGTGACATT	GCACAAAGAACGGGAAAACC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.204964	76089	76290	387-427	1.000000	79.9
rps12-clpP_p3	rps12-clpP	3	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	20	20	55.0	50.0	57.9	57.9	2.3	0.0	0.234977	76089	76290	367-407	1.000000	79.8
rps12-clpP_p4	rps12-clpP	4	CTAGTACATGTTCCTCGGCG	GCACAAAGAACGGGAAAACC	20	20	55.0	50.0	57.9	58.2	2.3	0.0	0.273298	76089	76290	426-466	1.000000	79.2
rps12-clpP_p5	rps12-clpP	5	CAAGAGCGGGGGATTTAGTG	TATGTCAGCAACAGAAGCCC	20	20	55.0	50.0	58.3	57.9	0.0	0.0	0.445441	76089	76290	333-373	1.000000	78.2
clpP-psbB_p1	clpP-psbB	1	TGAATCGTCTTCCCCAGGAT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.357718	78398	78921	598-605	1.000000	79.3
clpP-psbB_p2	clpP-psbB	2	TGAATCGTCTTCCCCAGGAT	CAAACGACCGGGATCATTCA	20	20	50.0	50.0	58.1	58.3	0.0	6.3	0.387485	78398	78921	635-642	1.000000	78.9
clpP-psbB_p3	clpP-psbB	3	TTGAATCGTCTTCCCCAGGA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.585556	78398	78921	599-606	1.000000	77.4
clpP-psbB_p4	clpP-psbB	4	TGAATCGTCTTCCCCAGGAT	ACACGATACCAAGGCAAACC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.590102	78398	78921	601-608	1.000000	77.4
clpP-psbB_p5	clpP-psbB	5	TGAATCGTCTTCCCCAGGAT	AAACGACCGGGATCATTCAA	20	20	50.0	45.0	58.1	57.5	0.0	6.3	0.603440	78398	78921	634-641	1.000000	77.2
petD-rpoA_p1	petD-rpoA	1	GATTGGTACTGCAGTAGCCC	TCGCATAGACGATGTAAAACA	20	21	55.0	38.1	58.0	55.5	39.1	0.0	3.575779	84305	84515	399-399	1.000000	53.4
petD-rpoA_p2	petD-rpoA	2	GCAGTAGCCCTTTGGTTAGG	TCGCATAGACGATGTAAAACA	20	21	55.0	38.1	58.2	55.5	0.0	0.0	3.779268	84305	84515	389-389	1.000000	51.8
petD-rpoA_p3	petD-rpoA	3	GGTACTGCAGTAGCCCTTTG	TCGCATAGACGATGTAAAACA	20	21	55.0	38.1	58.3	55.5	35.3	0.0	3.791639	84305	84515	395-395	1.000000	51.7
petD-rpoA_p4	petD-rpoA	4	GTACTGCAGTAGCCCTTTGG	TCGCATAGACGATGTAAAACA	20	21	55.0	38.1	58.3	55.5	26.7	0.0	3.791639	84305	84515	394-394	1.000000	51.7
petD-rpoA_p5	petD-rpoA	5	GATTGGTACTGCAGTAGCCC	TTCGCATAGACGATGTAAAACA	20	22	55.0	36.4	58.0	56.2	39.1	0.0	3.827667	84305	84515	400-400	1.000000	51.4
rpl16_p1	rpl16	1	GGCATCCCCAAGGTCTTTTT	ATTGAATAGACCCGCGGTTC	20	20	50.0	50.0	58.4	58.1	0.0	26.1	0.406026	88089	89891	1868-1909	1.000000	80.4
rpl16_p2	rpl16	2	GGCATCCCCAAGGTCTTTTT	GATTGAATAGACCCGCGGTT	20	20	50.0	50.0	58.4	58.1	0.0	26.1	0.406026	88089	89891	1869-1910	1.000000	80.4
rpl16_p3	rpl16	3	GGCATCCCCAAGGTCTTTTT	CGCGGTTCGATTGATGTAGT	20	20	50.0	50.0	58.4	58.4	0.0	0.0	0.798927	88089	89891	1856-1897	1.000000	77.2
rpl16_p4	rpl16	4	GCATCCCCAAGGTCTTTTTC	ATTGAATAGACCCGCGGTTC	20	20	50.0	50.0	57.0	58.1	0.0	26.1	1.088957	88089	89891	1867-1908	1.000000	74.9
rpl16_p5	rpl16	5	GCATCCCCAAGGTCTTTTTC	GATTGAATAGACCCGCGGTT	20	20	50.0	50.0	57.0	58.1	0.0	26.1	1.088957	88089	89891	1868-1909	1.000000	74.9
rpl16-rps3_p1	rpl16-rps3	1	TAAAAACCCAACGAGTCGCA	GGGTAGAGTTCCTTTACAAACCA	20	23	45.0	43.5	58.0	58.0	13.6	0.0	3.072308	89892	90099	338-356	1.000000	62.2
rpl16-rps3_p2	rpl16-rps3	2	TAAAAACCCAACGAGTCGCA	AGGGTAGAGTTCCTTTACAAACC	20	23	45.0	43.5	58.0	57.8	13.6	0.0	3.287795	89892	90099	339-357	1.000000	60.5
rpl16-rps3_p3	rpl16-rps3	3	TAAAAACCCAACGAGTCGCA	GGGTAGAGTTCCTTTACAAACC	20	22	45.0	45.5	58.0	56.4	13.6	0.0	3.691302	89892	90099	338-356	1.000000	57.2
rpl16-rps3_p4	rpl16-rps3	4	TAAAAACCCAACGAGTCGCA	TCCTTTACAAACCATTCAAGCT	20	22	45.0	36.4	58.0	56.2	13.6	0.0	3.879982	89892	90099	329-347	1.000000	55.8
rpl16-rps3_p5	rpl16-rps3	5	CCAACGAGTCGCACACTAAG	GGGTAGAGTTCCTTTACAAACCA	20	23	55.0	43.5	58.9	58.0	13.6	0.0	3.962423	89892	90099	331-349	1.000000	55.1
trnI-GAU-trnA_UGC_p1	trnI-GAU-trnA_UGC	1	TGATTTACTTCACGGGCGAG	CAACGACCCAATTGCAAGAG	20	20	50.0	50.0	58.0	57.7	0.0	4.9	0.341137	110861	110924	161-161	1.000000	78.3
trnI-GAU-trnA_UGC_p2	trnI-GAU-trnA_UGC	2	TGATTTACTTCACGGGCGAG	CCGCCTGGACAATTAGACAT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.336646	110861	110924	195-195	1.000000	78.1
trnI-GAU-trnA_UGC_p3	trnI-GAU-trnA_UGC	3	AGGTCTCTGGTTCAAGTCCA	CAACGACCCAATTGCAAGAG	20	20	50.0	50.0	57.9	57.7	0.0	4.9	0.439708	110861	110924	143-143	1.000000	77.6
trnI-GAU-trnA_UGC_p4	trnI-GAU-trnA_UGC	4	AGGTCTCTGGTTCAAGTCCA	CCGCCTGGACAATTAGACAT	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.435217	110861	110924	177-177	1.000000	77.4
trnI-GAU-trnA_UGC_p5	trnI-GAU-trnA_UGC	5	TGATTTACTTCACGGGCGAG	AGACATCCAACCCGTAATCG	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.601486	110861	110924	181-181	1.000000	76.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GCGAAATCCCTTTCTACAGGT	AGCGTGTCTACCGATTTCAC	21	20	47.6	50.0	58.0	58.0	0.0	0.0	1.020598	122125	123143	962-1098	1.000000	78.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCGAAATCCCTTTCTACAGGT	CAGCGTGTCTACCGATTTCA	21	20	47.6	50.0	58.0	58.0	0.0	0.0	1.021969	122125	123143	963-1099	1.000000	78.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGAAATCCCTTTCTACAGGT	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	58.0	57.9	0.0	0.0	1.137045	122125	123143	972-1108	1.000000	77.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGAAATCCCTTTCTACAGGT	CCTAAGAGCAGCGTGTCTAC	21	20	47.6	55.0	58.0	57.8	0.0	0.0	1.209063	122125	123143	971-1107	1.000000	76.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGAAATCCCTTTCTACAGGT	CTAAGAGCAGCGTGTCTACC	21	20	47.6	55.0	58.0	57.8	0.0	0.0	1.209063	122125	123143	970-1106	1.000000	76.7
trnA-UGC-trnI-GAU_p1	trnA-UGC-trnI-GAU	1	CAACGACCCAATTGCAAGAG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	4.9	0.0	0.341137	144664	144727	161-161	1.000000	78.3
trnA-UGC-trnI-GAU_p2	trnA-UGC-trnI-GAU	2	CCGCCTGGACAATTAGACAT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.336646	144664	144727	195-195	1.000000	78.1
trnA-UGC-trnI-GAU_p3	trnA-UGC-trnI-GAU	3	CAACGACCCAATTGCAAGAG	AGGTCTCTGGTTCAAGTCCA	20	20	50.0	50.0	57.7	57.9	4.9	0.0	0.439708	144664	144727	143-143	1.000000	77.6
trnA-UGC-trnI-GAU_p4	trnA-UGC-trnI-GAU	4	CCGCCTGGACAATTAGACAT	AGGTCTCTGGTTCAAGTCCA	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.435217	144664	144727	177-177	1.000000	77.4
trnA-UGC-trnI-GAU_p5	trnA-UGC-trnI-GAU	5	AGACATCCAACCCGTAATCG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	57.4	58.0	0.0	0.0	0.601486	144664	144727	181-181	1.000000	76.1