primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	AATCCACTGCCTTAATCCGC	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	58.3	53.8	0.0	0.0	4.559014	92	552	506-535	1.000000	55.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CCTTAATCCGCTTGGCTACA	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	57.7	53.8	0.0	0.0	4.564935	92	552	497-526	1.000000	55.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ATGAGTTATGGGCGAACGAC	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	58.1	53.8	0.0	0.0	4.303419	92	552	80-585	0.928571	54.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CGCATGGTGGATTCACAATC	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	57.5	53.8	6.4	0.0	4.719576	92	552	522-551	1.000000	54.2
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	TAATCCGCTTGGCTACATCC	GAAGCTCCATCTACAAATGG	20	20	50.0	45.0	57.5	53.8	0.0	0.0	4.783129	92	552	494-523	1.000000	53.7
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	TGGAAAGAAATTGCGTCCAA	20	20	50.0	40.0	58.0	56.2	0.0	9.0	1.876361	11239	11416	295-318	1.000000	77.0
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	TGGAAAGAAATTGCGTCCAA	20	20	55.0	40.0	58.1	56.2	0.0	9.0	1.905197	11239	11416	298-321	1.000000	76.8
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	11239	11416	248-271	1.000000	75.3
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	AGCCTTCCAAGCTAACGATG	ATGGAAAGAAATTGCGTCCA	20	20	50.0	40.0	58.0	55.9	0.0	12.8	2.108559	11239	11416	296-319	1.000000	75.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	11239	11416	251-274	1.000000	75.1
rps2-rpoC2_p1	rps2-rpoC2	1	CTTCGTGCTGATCAACGAGA	GATTGGTTGAAGGGCCTGAA	20	20	50.0	50.0	58.0	58.1	18.9	6.9	0.094032	17859	19076	1767-2190	0.928571	88.4
rps2-rpoC2_p2	rps2-rpoC2	2	TCTTCGTGCTGATCAACGAG	GATTGGTTGAAGGGCCTGAA	20	20	50.0	50.0	58.0	58.1	18.9	6.9	0.094032	17859	19076	1768-2191	0.928571	88.4
rps2-rpoC2_p3	rps2-rpoC2	3	CTTCGTGCTGATCAACGAGA	GGGTCGAATCGATTGGTTGA	20	20	50.0	50.0	58.0	58.0	18.9	17.0	0.035930	17859	19076	1777-2200	0.785714	83.1
rps2-rpoC2_p4	rps2-rpoC2	4	TCTTCGTGCTGATCAACGAG	GGGTCGAATCGATTGGTTGA	20	20	50.0	50.0	58.0	58.0	18.9	17.0	0.035930	17859	19076	1778-2201	0.785714	83.1
rps2-rpoC2_p5	rps2-rpoC2	5	CTTATCGCAGCCCGTACTAC	GGGTCGAATCGATTGGTTGA	20	20	55.0	50.0	58.0	58.0	0.0	17.0	0.066221	17859	19076	1529-1982	0.785714	82.9
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	GGGTTAGTCTTTCTGGTGGG	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	57.5	58.1	0.0	0.0	0.550350	39585	39986	463-513	1.000000	87.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	GGGTTAGTCTTTCTGGTGGG	GAATCGAACCCGCATCTTCT	20	20	55.0	50.0	57.5	58.1	0.0	0.0	0.550350	39585	39986	464-514	1.000000	87.6
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GGGTTAGTCTTTCTGGTGGG	GAACCCGCATCTTCTCCTTG	20	20	55.0	55.0	57.5	58.6	0.0	0.0	1.118105	39585	39986	459-509	1.000000	83.1
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	AGTCTTTCTGGTGGGTATCCT	AATCGAACCCGCATCTTCTC	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.265719	39585	39986	458-508	1.000000	81.9
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	AGTCTTTCTGGTGGGTATCCT	GAATCGAACCCGCATCTTCT	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.265719	39585	39986	459-509	1.000000	81.9
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	GTTCAAATCCTGTCTCCGCA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.250264	40058	40245	232-241	1.000000	90.0
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	GTTCAAATCCTGTCTCCGCA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.250264	40058	40245	233-242	1.000000	90.0
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGAAGATGCGGGTTCGATTC	GTAGCTCGCAAGGCTCATAA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.283743	40058	40245	266-275	1.000000	89.7
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	AGAAGATGCGGGTTCGATTC	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.283743	40058	40245	265-274	1.000000	89.7
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	GAGAAGATGCGGGTTCGATT	TAGCTCGCAAGGCTCATAAC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.283743	40058	40245	266-275	1.000000	89.7
psaI-trnI-CAU_p1	psaI-trnI-CAU	1	ACAGATGAGATTGGGTCCCA	GGGGAGTGACATTGATCCAC	20	20	50.0	55.0	58.0	58.0	6.5	0.0	0.072974	47409	49197	1941-2824	0.642857	77.1
psaI-trnI-CAU_p2	psaI-trnI-CAU	2	TTTTAGCCATCCAGCGATCC	GGGGAGTGACATTGATCCAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.076804	47409	49197	2153-2996	0.571429	74.2
psaI-trnI-CAU_p3	psaI-trnI-CAU	3	GCCATTGCAATTGTCGGAAA	GTTGGGCGCTTTAACCATTC	20	20	45.0	50.0	57.9	58.0	9.8	7.6	0.086614	47409	49197	80-2939	0.500000	71.3
psaI-trnI-CAU_p4	psaI-trnI-CAU	4	ACAGATGAGATTGGGTCCCA	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	6.5	7.6	0.038052	47409	49197	1941-2868	0.357143	66.0
psaI-trnI-CAU_p5	psaI-trnI-CAU	5	TTTTAGCCATCCAGCGATCC	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	58.0	58.0	0.0	7.6	0.041882	47409	49197	2145-2672	0.357143	66.0
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	GCGTGTTGGGTCTTTGAAAC	GGCTTGAGATTATTAGAAATGCCC	20	24	50.0	41.7	58.2	58.0	0.0	0.0	4.183468	55517	57039	80-1675	1.000000	57.2
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	GCGTGTTGGGTCTTTGAAAC	AATGGGAATAAGGCTTGAGA	20	20	50.0	40.0	58.2	54.0	0.0	10.7	4.199842	55517	57039	80-1686	1.000000	57.0
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	GCGTGTTGGGTCTTTGAAAC	TGGGAATAAGGCTTGAGATT	20	20	50.0	40.0	58.2	54.0	0.0	10.7	4.199842	55517	57039	80-1684	1.000000	57.0
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	AGAAGGTCTACGGTTCGAGT	GGCTTGAGATTATTAGAAATGCCC	20	24	50.0	41.7	57.8	58.0	0.0	0.0	4.215872	55517	57039	80-1611	0.928571	53.8
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	AGAAGGTCTACGGTTCGAGT	TGGGAATAAGGCTTGAGATT	20	20	50.0	40.0	57.8	54.0	0.0	10.7	4.232246	55517	57039	80-1620	0.928571	53.6
trnL-UAA-trnT-UGU_p1	trnL-UAA-trnT-UGU	1	ACCAATTTCGCCATATCCCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.137364	60381	61066	80-780	1.000000	89.1
trnL-UAA-trnT-UGU_p2	trnL-UAA-trnT-UGU	2	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.202173	60381	61066	80-788	1.000000	88.5
trnL-UAA-trnT-UGU_p3	trnL-UAA-trnT-UGU	3	ACCAATTTCGCCATATCCCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.0	57.8	0.0	15.5	0.245117	60381	61066	80-812	1.000000	88.0
trnL-UAA-trnT-UGU_p4	trnL-UAA-trnT-UGU	4	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	20	20	50.0	50.0	58.1	57.8	0.0	15.5	0.309926	60381	61066	80-820	1.000000	87.4
trnL-UAA-trnT-UGU_p5	trnL-UAA-trnT-UGU	5	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	20	20	50.0	55.0	58.2	57.9	10.2	0.0	0.372548	60381	61066	80-794	1.000000	87.1
trnS-GGA-ycf3_p1	trnS-GGA-ycf3	1	CCCTCGGTAAACAAAAGCCT	CATTTATCCGCGATCTAGGCA	20	21	50.0	47.6	58.1	58.0	0.0	8.2	1.134847	62529	63348	80-970	1.000000	79.4
trnS-GGA-ycf3_p2	trnS-GGA-ycf3	2	ACGGAAAGAGAGGGATTCGA	CATTTATCCGCGATCTAGGCA	20	21	50.0	47.6	58.2	58.0	0.0	8.2	1.191833	62529	63348	80-991	1.000000	78.9
trnS-GGA-ycf3_p3	trnS-GGA-ycf3	3	TTCCAATGCTACGCCTTGAA	CATTTATCCGCGATCTAGGCA	20	21	45.0	47.6	57.8	58.0	5.6	8.2	1.233148	62529	63348	80-941	1.000000	78.8
trnS-GGA-ycf3_p4	trnS-GGA-ycf3	4	ACCCTCGGTAAACAAAAGCC	CATTTATCCGCGATCTAGGCA	20	21	50.0	47.6	58.4	58.0	0.0	8.2	1.427808	62529	63348	80-971	1.000000	77.1
trnS-GGA-ycf3_p5	trnS-GGA-ycf3	5	CCCTCGGTAAACAAAAGCCT	TTTATCCGCGATCTAGGCAT	20	20	50.0	45.0	58.1	56.6	0.0	8.2	1.514239	62529	63348	80-968	1.000000	76.4
ycf3-cemA_p1	ycf3-cemA	1	TTGAAGATCACGAGGCGTTT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	0.0	0.0	0.240462	65329	66252	80-1090	1.000000	88.5
ycf3-cemA_p2	ycf3-cemA	2	TTGGTTGAAGATCACGAGGC	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.268234	65329	66252	80-1094	1.000000	88.3
ycf3-cemA_p3	ycf3-cemA	3	TGGTTTGATCAAGCAGCTGA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.7	58.1	20.4	0.0	0.351778	65329	66252	80-1168	1.000000	87.5
ycf3-cemA_p4	ycf3-cemA	4	GGAAACAGGCTATAGCGCTT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	18.7	0.0	0.121717	65329	66252	80-1136	0.928571	86.3
ycf3-cemA_p5	ycf3-cemA	5	GGAAACAGGCTATAGCGCTT	CCACCAGGGCAAAAAGACTA	20	20	50.0	50.0	58.0	57.7	18.7	0.0	0.331438	65329	66252	80-1129	0.928571	84.6
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTCCCGGATGCTTTGACA	CATCTTTCCCCTTGGCCAT	20	19	45.0	52.6	58.0	57.4	0.7	6.1	1.633126	72717	73767	80-1102	1.000000	78.9
psaJ-rpl33_p2	psaJ-rpl33	2	TGTGGCACCAGTCCTAAGTA	CATCTTTCCCCTTGGCCAT	20	19	50.0	52.6	58.0	57.4	16.3	6.1	1.639774	72717	73767	80-1170	1.000000	78.9
psaJ-rpl33_p3	psaJ-rpl33	3	TCCCGGATGCTTTGACATTT	CATCTTTCCCCTTGGCCAT	20	19	45.0	52.6	57.8	57.4	0.7	6.1	1.835795	72717	73767	80-1099	1.000000	77.3
psaJ-rpl33_p4	psaJ-rpl33	4	TTCCCGGATGCTTTGACATT	CATCTTTCCCCTTGGCCAT	20	19	45.0	52.6	57.8	57.4	0.7	6.1	1.835795	72717	73767	80-1100	1.000000	77.3
psaJ-rpl33_p5	psaJ-rpl33	5	TTTCCCGGATGCTTTGACAT	CATCTTTCCCCTTGGCCAT	20	19	45.0	52.6	57.8	57.4	0.7	6.1	1.835795	72717	73767	80-1101	1.000000	77.3
rps19_p1	rps19	1	TAGGAGCGTCCACGAATTTG	TTGATTCTTCGTCGCCGTAG	20	20	50.0	50.0	58.0	58.1	2.6	0.0	0.100439	89473	89823	80-643	1.000000	91.2
rps19_p2	rps19	2	TAGGAGCGTCCACGAATTTG	ATCCCAAAACTGCTCAGCAA	20	20	50.0	45.0	58.0	58.0	2.6	0.0	0.004934	89473	89823	80-896	1.000000	90.6
rps19_p3	rps19	3	GAATGGTGACCTTGGGTTGT	ATCCCAAAACTGCTCAGCAA	20	20	50.0	45.0	58.0	58.0	2.1	0.0	0.004976	89473	89823	80-1304	1.000000	90.6
rps19_p4	rps19	4	AGCGTCCACGAATTTGATCA	ATCCCAAAACTGCTCAGCAA	20	20	45.0	45.0	57.9	58.0	2.6	0.0	0.093658	89473	89823	80-892	1.000000	89.9
rps19_p5	rps19	5	TGCTGCTGCAGAATAAACCA	ATCCCAAAACTGCTCAGCAA	20	20	45.0	45.0	58.1	58.0	32.3	0.0	0.092008	89473	89823	80-978	0.857143	82.8
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	ATTGGCATGCATCCAGTAGG	TGCGCGTGAAAGTAGGATAC	20	20	50.0	50.0	58.0	58.1	21.4	8.4	0.091606	91868	92019	80-602	1.000000	91.3
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GTTGGGCGCTTTAACCATTC	TGCGCGTGAAAGTAGGATAC	20	20	50.0	50.0	58.0	58.1	7.6	8.4	0.091928	91868	92019	80-553	1.000000	91.3
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	ATTGGCATGCATCCAGTAGG	GTGCGCGTGAAAGTAGGATA	20	20	50.0	50.0	58.0	58.1	21.4	8.4	0.091606	91868	92019	80-603	0.928571	88.4
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	GTGCGCGTGAAAGTAGGATA	20	20	50.0	50.0	58.0	58.1	7.6	8.4	0.091928	91868	92019	80-554	0.928571	88.4
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	ATTGGCATGCATCCAGTAGG	CCCGTTCTTTGTTCGGATCT	20	20	50.0	50.0	58.0	57.9	21.4	4.4	0.103641	91868	92019	80-387	0.857143	85.5
ndhF-rpl32_p1	ndhF-rpl32	1	GCCCACATACGACGAAGATT	AGGTCGATGGGAAAATGAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.108190	121817	122310	80-1063	0.428571	63.5
ndhF-rpl32_p2	ndhF-rpl32	2	GCCCACATACGACGAAGATT	GTCGATGGGAAAATGAGCCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.108190	121817	122310	80-1061	0.428571	63.5
ndhF-rpl32_p3	ndhF-rpl32	3	CAAGAGGTCGTGTGAACCAA	AGGTCGATGGGAAAATGAGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096018	121817	122310	80-1452	0.428571	62.1
ndhF-rpl32_p4	ndhF-rpl32	4	CAAGAGGTCGTGTGAACCAA	GTCGATGGGAAAATGAGCCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.096018	121817	122310	80-1450	0.428571	62.1
ndhF-rpl32_p5	ndhF-rpl32	5	ACAAACGCCTTTTGACAAGC	GTCGATGGGAAAATGAGCCT	20	20	45.0	50.0	58.1	58.0	11.5	0.0	0.122879	121817	122310	80-1478	0.428571	61.8
rps15-ycf1_p1	rps15-ycf1	1	GACCCTCTGTTTTCTTTGTTTTCT	TGGCAGTCGTTTCAGTATGT	24	20	37.5	45.0	57.8	57.2	0.0	0.0	5.040673	132598	133012	80-561	0.928571	49.1
rps15-ycf1_p2	rps15-ycf1	2	ACCCTCTGTTTTCTTTGTTTTCT	TGGCAGTCGTTTCAGTATGT	23	20	34.8	45.0	56.7	57.2	0.0	0.0	5.090597	132598	133012	80-560	0.928571	49.1
rps15-ycf1_p3	rps15-ycf1	3	GACCCTCTGTTTTCTTTGTT	TGGCAGTCGTTTCAGTATGT	20	20	40.0	45.0	53.6	57.2	0.0	0.0	5.208316	132598	133012	80-561	0.928571	49.1
rps15-ycf1_p4	rps15-ycf1	4	GACCCTCTGTTTTCTTTGTTT	TGGCAGTCGTTTCAGTATGT	21	20	38.1	45.0	54.5	57.2	0.0	0.0	5.326650	132598	133012	80-561	0.928571	49.1
rps15-ycf1_p5	rps15-ycf1	5	GACCCTCTGTTTTCTTTGTTTTCT	TGGCAGTCGTTTCAGTATGTT	24	21	37.5	42.9	57.8	57.9	0.0	0.0	5.329309	132598	133012	80-561	0.928571	49.1
ycf1_p1	ycf1	1	TCCGGAACAAGCAAATCCAA	CTGTTGATGGATATGGCGGG	20	20	45.0	55.0	58.0	58.5	0.0	0.0	0.484672	133951	134070	80-1388	0.500000	68.1
ycf1_p2	ycf1	2	TAACGTCTTAAGGCCGCATC	GCGGGGGAGATCTCTATCAA	20	20	50.0	55.0	58.1	58.4	13.2	21.8	0.435659	133951	134070	80-812	0.428571	62.2
ycf1_p3	ycf1	3	TTCAATCTTACGAGCCGACG	GCGGGGGAGATCTCTATCAA	20	20	50.0	55.0	58.1	58.4	1.5	21.8	0.462082	133951	134070	80-1035	0.428571	61.9
ycf1_p4	ycf1	4	TCCGGAACAAGCAAATCCAA	GCGGGGGAGATCTCTATCAA	20	20	45.0	55.0	58.0	58.4	0.0	21.8	0.377933	133951	134070	80-1373	0.428571	61.8
ycf1_p5	ycf1	5	TTCCGGAACAAGCAAATCCA	GCGGGGGAGATCTCTATCAA	20	20	45.0	55.0	58.0	58.4	14.3	21.8	0.377933	133951	134070	80-1374	0.428571	61.8