primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ndhF-rpl32	1	CGCCCACACGATTAGTTACA	TCTGCCCTTACCGAGAAAGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.067930	110234	111312	2067-2067	0.500000	59.5
ndhF-rpl32_p2	ndhF-rpl32	2	CGCCCACACGATTAGTTACA	AAAAAGACTCCCCACCACAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.068317	110234	111312	2010-2010	0.500000	59.5
ndhF-rpl32_p3	ndhF-rpl32	3	GTTGCCGTCGGAAAAAGAAG	TCTGCCCTTACCGAGAAAGA	20	20	50.0	50.0	58.0	58.1	17.5	0.0	0.099393	110234	111312	1609-1609	0.500000	59.2
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	AAAAAGACTCCCCACCACAC	20	20	50.0	50.0	58.0	57.9	17.5	0.0	0.099780	110234	111312	1552-1552	0.500000	59.2
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	TCTGCCCTTACCGAGAAAGA	20	20	45.0	50.0	58.0	58.1	17.5	0.0	0.110682	110234	111312	1607-1607	0.500000	59.1
rpl32-trnL_p1	rpl32-trnL	1	TGGCAGCGTTAAAAGCTTTG	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.140570	111478	112265	906-906	0.500000	58.9
rpl32-trnL_p2	rpl32-trnL	2	TGGCAGCGTTAAAAGCTTTG	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.141941	111478	112265	907-907	0.500000	58.9
rpl32-trnL_p3	rpl32-trnL	3	GGCAGCGTTAAAAGCTTTGT	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.142032	111478	112265	905-905	0.500000	58.9
rpl32-trnL_p4	rpl32-trnL	4	GGCAGCGTTAAAAGCTTTGT	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.9	58.0	13.5	0.0	0.143402	111478	112265	906-906	0.500000	58.9
rpl32-trnL_p5	rpl32-trnL	5	TGGCAGCGTTAAAAGCTTTG	GAAGTAGCAAAGCCTTGGGT	20	20	45.0	50.0	57.9	58.1	13.5	0.0	0.221683	111478	112265	1413-1413	0.500000	58.2
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	112458	113426	1564-1564	0.500000	59.6
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	112458	113426	1563-1563	0.500000	59.6
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	AGTGATTCACGTGGTTCTCG	20	20	50.0	50.0	58.0	57.9	0.0	17.0	0.073823	112458	113426	1247-1247	0.500000	59.4
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	AGTGATTCACGTGGTTCTCG	20	20	50.0	50.0	58.0	57.9	0.0	17.0	0.075193	112458	113426	1246-1246	0.500000	59.4
ccsA_p5	ccsA	5	TCTTTCTCGGTAAGGGCAGA	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.103232	112458	113426	2114-2114	0.500000	59.2
ccsA-ndhD_p1	ccsA-ndhD	1	ACCCAAGGCTTTGCTACTTC	CTCCCGGCCAACGTAATTTA	20	20	50.0	50.0	58.1	58.0	2.4	2.2	0.115946	113427	113694	1533-1533	0.500000	59.1
ccsA-ndhD_p2	ccsA-ndhD	2	CCCAAGGCTTTGCTACTTCA	CTCCCGGCCAACGTAATTTA	20	20	50.0	50.0	58.1	58.0	2.4	2.2	0.117304	113427	113694	1532-1532	0.500000	59.1
ccsA-ndhD_p3	ccsA-ndhD	3	ACCCAAGGCTTTGCTACTTC	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.1	58.0	2.4	0.0	0.125196	113427	113694	1057-1057	0.500000	59.0
ccsA-ndhD_p4	ccsA-ndhD	4	CCCAAGGCTTTGCTACTTCA	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.1	58.0	2.4	0.0	0.126553	113427	113694	1056-1056	0.500000	59.0
ccsA-ndhD_p5	ccsA-ndhD	5	ACCCAAGGCTTTGCTACTTC	CATGGCTTTATTGGTGCTGC	20	20	50.0	50.0	58.1	58.1	2.4	0.0	0.158375	113427	113694	1405-1405	0.500000	58.7
ndhD_p1	ndhD	1	CGAGAACCACGTGAATCACT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.9	58.0	19.3	0.0	0.081449	113695	115215	1936-1936	0.500000	59.3
ndhD_p2	ndhD	2	CGAGAACCACGTGAATCACT	ACCTTATTTTGGGGCACAGG	20	20	50.0	50.0	57.9	58.1	19.3	0.0	0.128802	113695	115215	1748-1748	0.500000	59.0
ndhD_p3	ndhD	3	CGAGAACCACGTGAATCACT	CCTTATTTTGGGGCACAGGT	20	20	50.0	50.0	57.9	58.1	19.3	0.0	0.128802	113695	115215	1747-1747	0.500000	59.0
ndhD_p4	ndhD	4	TCGTGTGATTAGCCTAGGGT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.8	58.0	19.2	0.0	0.170023	113695	115215	2309-2309	0.500000	58.6
ndhD_p5	ndhD	5	CGAGAACCACGTGAATCACT	CAGAAACCCCTACTTGAGCC	20	20	50.0	55.0	57.9	57.9	19.3	0.0	0.181335	113695	115215	1803-1803	0.500000	58.5
ndhD-psaC_p1	ndhD-psaC	1	CTCCCGCCGTGTACAAAATA	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028393	115216	115311	760-760	0.500000	59.8
ndhD-psaC_p2	ndhD-psaC	2	TTCCAAAGCCACAGGATACG	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.123406	115216	115311	870-870	0.500000	59.0
ndhD-psaC_p3	ndhD-psaC	3	TCCTCCCCACATGCATAGAA	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.123523	115216	115311	711-711	0.500000	59.0
ndhD-psaC_p4	ndhD-psaC	4	TTCCTCCCCACATGCATAGA	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.123523	115216	115311	712-712	0.500000	59.0
ndhD-psaC_p5	ndhD-psaC	5	ACCATATAACCCGACACCCA	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.133148	115216	115311	807-807	0.500000	58.9
psaC_p1	psaC	1	CTCCCGCCGTGTACAAAATA	TGGCACTGTCAATCCAGAAG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.202531	115312	115557	1020-1020	0.500000	58.4
psaC_p2	psaC	2	CTCCCGCCGTGTACAAAATA	GGCACTGTCAATCCAGAAGT	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.204065	115312	115557	1019-1019	0.500000	58.4
psaC_p3	psaC	3	ACCTGTGCCCCAAAATAAGG	TGGCACTGTCAATCCAGAAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.252403	115312	115557	469-469	0.500000	58.0
psaC_p4	psaC	4	CCTGTGCCCCAAAATAAGGT	TGGCACTGTCAATCCAGAAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.252403	115312	115557	468-468	0.500000	58.0
psaC_p5	psaC	5	CCTGTGCCCCAAAATAAGGT	GGCACTGTCAATCCAGAAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.253937	115312	115557	467-467	0.500000	58.0
ndhE_p1	ndhE	1	TCCTCTGTTCTTGGAGCAGA	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.0	58.0	12.1	0.0	0.020826	115800	116105	1157-1157	0.500000	59.8
ndhE_p2	ndhE	2	ACCTGTGCCCCAAAATAAGG	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.068179	115800	116105	1346-1346	0.500000	59.5
ndhE_p3	ndhE	3	CCTGTGCCCCAAAATAAGGT	TGGAGTAACTTCCGTGGTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.068179	115800	116105	1345-1345	0.500000	59.5
ndhE_p4	ndhE	4	GGCTCAAGTAGGGGTTTCTG	TGGAGTAACTTCCGTGGTCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.120713	115800	116105	1290-1290	0.500000	59.0
ndhE_p5	ndhE	5	AGAAAAACCTGTGCCCCAAA	TGGAGTAACTTCCGTGGTCT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.132598	115800	116105	1352-1352	0.500000	58.9
ndhG_p1	ndhG	1	CTTCTGGATTGACAGTGCCA	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.282900	116334	116864	1920-1920	0.500000	57.7
ndhG_p2	ndhG	2	ACTTCTGGATTGACAGTGCC	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.284434	116334	116864	1921-1921	0.500000	57.7
ndhG_p3	ndhG	3	CTTCTGGATTGACAGTGCCA	TAGGTGACGAGAATCCCGTT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.346226	116334	116864	1474-1474	0.500000	57.2
ndhG_p4	ndhG	4	ACTTCTGGATTGACAGTGCC	TAGGTGACGAGAATCCCGTT	20	20	50.0	50.0	57.8	58.2	0.0	0.0	0.347759	116334	116864	1475-1475	0.500000	57.2
ndhG_p5	ndhG	5	CTTCTGGATTGACAGTGCCA	ATTGATCACATCCGAGCGTT	20	20	50.0	45.0	57.8	57.7	0.0	0.0	0.505519	116334	116864	1921-1921	0.500000	56.0
ndhI_p1	ndhI	1	AGACCACGGAAGTTACTCCA	AGCCGAGTGTGAACGATTAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	117235	117735	1684-1684	0.500000	59.9
ndhI_p2	ndhI	2	AGACCACGGAAGTTACTCCA	GGTTCTGCAATAGGGATGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.018488	117235	117735	1846-1846	0.500000	59.9
ndhI_p3	ndhI	3	AGACCACGGAAGTTACTCCA	TTTGTGACCTTTGAGGAGCC	20	20	50.0	50.0	58.0	58.0	0.0	0.1	0.024114	117235	117735	1890-1890	0.500000	59.8
ndhI_p4	ndhI	4	AGACCACGGAAGTTACTCCA	GACCTTTGAGGAGCCGTATG	20	20	50.0	55.0	58.0	58.1	0.0	0.1	0.064045	117235	117735	1885-1885	0.500000	59.5
ndhI_p5	ndhI	5	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064045	117235	117735	1379-1379	0.500000	59.5
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	117815	120042	2737-2737	0.500000	59.4
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	TTCAACAGGCCCTAGAAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077069	117815	120042	2738-2738	0.500000	59.4
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	117815	120042	2810-2810	0.500000	59.2
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	117815	120042	2811-2811	0.500000	59.2
ndhA_p5	ndhA	5	AAACGCTCGGATGTGATCAA	TCAACAGGCCCTAGAAGGAA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.132169	117815	120042	2824-2824	0.500000	58.9
ndhH_p1	ndhH	1	CCAATGGTCCTGCGTATTCA	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136720	120044	121222	1572-1572	0.500000	58.9
ndhH_p2	ndhH	2	CCAATGGTCCTGCGTATTCA	GGGAGCCGTTTGAGTATGTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.146382	120044	121222	2010-2010	0.500000	58.8
ndhH_p3	ndhH	3	GTTGTTGTATACCCGCGGAT	CGCTTGCTGGCTTATTTGTC	20	20	50.0	50.0	58.1	58.1	30.7	0.0	0.155731	120044	121222	1542-1542	0.500000	58.8
ndhH_p4	ndhH	4	CCTGACATAAGAAGGCCGAC	CGCTTGCTGGCTTATTTGTC	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.158438	120044	121222	1807-1807	0.500000	58.7
ndhH_p5	ndhH	5	GACATAAGAAGGCCGACAGG	CGCTTGCTGGCTTATTTGTC	20	20	55.0	50.0	58.1	58.1	0.0	0.0	0.158438	120044	121222	1804-1804	0.500000	58.7
rps15_p1	rps15	1	TGAGGTAAATCAGCAGCGAC	TACCGAAACCCCAAAAACGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.060033	121314	121592	1494-1494	0.500000	59.5
rps15_p2	rps15	2	TGAGGTAAATCAGCAGCGAC	AATTATGGAACCTGCCCGTC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.062927	121314	121592	1603-1603	0.500000	59.5
rps15_p3	rps15	3	TGAGGTAAATCAGCAGCGAC	TATGGAACCTGCCCGTCTAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107255	121314	121592	1600-1600	0.500000	59.1
rps15_p4	rps15	4	AACACCATGCATTGATGGGT	TACCGAAACCCCAAAAACGT	20	20	45.0	45.0	58.1	58.0	8.9	0.0	0.108511	121314	121592	1063-1063	0.500000	59.1
rps15_p5	rps15	5	AACACCATGCATTGATGGGT	AATTATGGAACCTGCCCGTC	20	20	45.0	50.0	58.1	57.9	8.9	0.0	0.111405	121314	121592	1172-1172	0.500000	59.1
rps15-ycf1_p1	rps15-ycf1	1	CCGCAGACCTCTCTGAGATA	TACCGAAACCCCAAAAACGT	20	20	55.0	45.0	58.0	58.0	8.0	0.0	0.075623	121593	121799	789-789	0.500000	59.4
rps15-ycf1_p2	rps15-ycf1	2	CCGCAGACCTCTCTGAGATA	AATTATGGAACCTGCCCGTC	20	20	55.0	50.0	58.0	57.9	8.0	0.0	0.078517	121593	121799	898-898	0.500000	59.4
rps15-ycf1_p3	rps15-ycf1	3	AACACCATGCATTGATGGGT	TACCGAAACCCCAAAAACGT	20	20	45.0	45.0	58.1	58.0	8.9	0.0	0.108511	121593	121799	1063-1063	0.500000	59.1
rps15-ycf1_p4	rps15-ycf1	4	AACACCATGCATTGATGGGT	AATTATGGAACCTGCCCGTC	20	20	45.0	50.0	58.1	57.9	8.9	0.0	0.111405	121593	121799	1172-1172	0.500000	59.1
rps15-ycf1_p5	rps15-ycf1	5	CCGCAGACCTCTCTGAGATA	TATGGAACCTGCCCGTCTAT	20	20	55.0	50.0	58.0	57.9	8.0	0.0	0.122845	121593	121799	895-895	0.500000	59.0
ycf1_p1	ycf1	1	TCTTCTTCCGCCTGTGAAAA	GACCGTCTCAAGGAAATCCC	20	20	45.0	55.0	57.4	58.0	0.0	0.0	0.656749	124316	124435	904-904	0.500000	54.7
ycf1_p2	ycf1	2	TCTTCTTCCGCCTGTGAAAA	GGACCGTCTCAAGGAAATCC	20	20	45.0	55.0	57.4	58.0	0.0	0.0	0.656749	124316	124435	905-905	0.500000	54.7
ycf1_p3	ycf1	3	TCTTCTTCCGCCTGTGAAAA	GGGACCGTCTCAAGGAAATC	20	20	45.0	55.0	57.4	58.0	0.0	0.0	0.656749	124316	124435	906-906	0.500000	54.7
ycf1_p4	ycf1	4	TTCTTCTTCCGCCTGTGAAA	GACCGTCTCAAGGAAATCCC	20	20	45.0	55.0	57.4	58.0	0.0	0.0	0.656749	124316	124435	905-905	0.500000	54.7
ycf1_p5	ycf1	5	TTCTTCTTCCGCCTGTGAAA	GGACCGTCTCAAGGAAATCC	20	20	45.0	55.0	57.4	58.0	0.0	0.0	0.656749	124316	124435	906-906	0.500000	54.7