primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TACAGAAGCGACCCCATAGG	CTAGTTCCGGGTTCGAATCC	20	20	55.0	55.0	58.6	57.8	0.0	18.0	0.810474	1542	1761	291-307	1.000000	80.2
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TACAGAAGCGACCCCATAGG	GAAAGCCGTGTGCAATGAAA	20	20	55.0	45.0	58.6	57.9	0.0	0.0	0.722736	1542	1761	376-392	1.000000	79.7
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	ATCCAGTTACAGAAGCGACC	CTAGTTCCGGGTTCGAATCC	20	20	50.0	55.0	57.3	57.8	0.0	18.0	0.895274	1542	1761	298-314	1.000000	79.5
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	ATCCAGTTACAGAAGCGACC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.3	57.9	0.0	0.0	0.807536	1542	1761	383-399	1.000000	78.9
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	AGTTACAGAAGCGACCCCAT	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	58.7	57.9	0.0	0.0	0.856118	1542	1761	379-395	1.000000	78.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	CTAGATCCTTGCCCCTGAGA	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	57.9	58.1	0.0	16.9	0.170201	6329	7239	1163-1176	1.000000	83.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CTAGATCCTTGCCCCTGAGA	CTTACAGCAGCTTGCCAAAC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.150194	6329	7239	1738-1751	1.000000	82.5
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TTTATTCGGATCCGCGAGAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.9	58.1	29.6	16.9	0.205924	6329	7239	1481-1494	1.000000	82.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ACTTTCTACTCGAGCTCCGT	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	58.2	57.9	13.6	0.0	0.232297	6329	7239	1707-1720	1.000000	81.9
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TTTATTCGGATCCGCGAGAC	CTTACAGCAGCTTGCCAAAC	20	20	50.0	50.0	57.9	57.9	29.6	0.0	0.185917	6329	7239	2056-2069	1.000000	81.7
atpI-atpH_p1	atpI-atpH	1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	57.9	58.0	0.0	1.4	0.167157	24846	26024	1209-1313	1.000000	83.9
atpI-atpH_p2	atpI-atpH	2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.241050	24846	26024	1265-1369	1.000000	83.2
atpI-atpH_p3	atpI-atpH	3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.277216	24846	26024	1225-1329	1.000000	83.0
atpI-atpH_p4	atpI-atpH	4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	57.9	58.2	0.0	1.4	0.312696	24846	26024	1210-1314	1.000000	82.7
atpI-atpH_p5	atpI-atpH	5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.319087	24846	26024	1269-1373	1.000000	82.6
psaA-ycf3_p1	psaA-ycf3	1	GCATCAGCATGTAGGTTCCA	TTCTGAAATTGCGGAGGCTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.132986	41034	41749	945-1006	1.000000	84.7
psaA-ycf3_p2	psaA-ycf3	2	AACGCTGCAAAGATCAATGC	TTCTGAAATTGCGGAGGCTT	20	20	45.0	45.0	58.0	58.1	14.0	0.0	0.103602	41034	41749	1293-1354	1.000000	83.8
psaA-ycf3_p3	psaA-ycf3	3	GTTATTCCAGATGCTCGCCA	TTCTGAAATTGCGGAGGCTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.136741	41034	41749	1239-1300	1.000000	83.7
psaA-ycf3_p4	psaA-ycf3	4	AACGCTGCAAAGATCAATGC	ATTCTGAAATTGCGGAGGCT	20	20	45.0	45.0	58.0	57.9	14.0	0.0	0.148091	41034	41749	1294-1355	1.000000	83.4
psaA-ycf3_p5	psaA-ycf3	5	CCCCGCTAAATGGTGATTCA	TTCTGAAATTGCGGAGGCTT	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.141635	41034	41749	1390-1451	1.000000	83.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AAAACCTGACCTGTTGACCC	CGGAAAACGTGATTTGGCTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.103619	45779	46343	1306-1375	1.000000	86.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AATAGTCGGAGCCATACCCA	CGGAAAACGTGATTTGGCTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.133363	45779	46343	1370-1439	1.000000	85.6
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	ATAGTCGGAGCCATACCCAA	CGGAAAACGTGATTTGGCTC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.133363	45779	46343	1369-1438	1.000000	85.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAACCTGACCTGTTGACCC	CTTCCATCGAGTCTCTGCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.147447	45779	46343	1368-1437	1.000000	85.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	TGTTATCCAGGCGCATTTCA	CGGAAAACGTGATTTGGCTC	20	20	45.0	50.0	57.9	58.0	10.8	0.0	0.157544	45779	46343	1338-1407	1.000000	85.5
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47296	47914	672-722	1.000000	75.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47296	47914	671-721	1.000000	75.1
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47296	47914	679-729	1.000000	74.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47296	47914	673-723	1.000000	73.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47296	47914	672-722	1.000000	72.1
rbcL_p1	rbcL	1	TCATGAGTTGTAGGGAGGGA	TCCAAAGTATCCATTGTCTG	20	20	50.0	40.0	57.1	52.6	0.0	0.0	6.366820	53693	55123	1478-1478	1.000000	41.6
rbcL_p2	rbcL	2	TCATGAGTTGTAGGGAGGGA	TCCTTATCCAAAGTATCCATTGTCT	20	25	50.0	36.0	57.1	57.5	0.0	0.0	6.392245	53693	55123	1484-1484	1.000000	41.6
rbcL_p3	rbcL	3	TCATGAGTTGTAGGGAGGGA	CCTTATCCAAAGTATCCATTGTCTG	20	25	50.0	40.0	57.1	57.5	0.0	0.0	6.442634	53693	55123	1483-1483	1.000000	41.6
rbcL_p4	rbcL	4	TTCATGAGTTGTAGGGAGGGA	TCCAAAGTATCCATTGTCTG	21	20	47.6	40.0	57.8	52.6	0.0	0.0	6.625748	53693	55123	1479-1479	1.000000	41.6
rbcL_p5	rbcL	5	TTCATGAGTTGTAGGGAGGGA	TCCTTATCCAAAGTATCCATTGTCT	21	25	47.6	36.0	57.8	57.5	0.0	0.0	6.651173	53693	55123	1485-1485	1.000000	41.6
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	AGAGAGAAATCCACCAGGGT	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.380701	58899	59771	1043-1056	1.000000	80.2
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	AGAGAGAAATCCACCAGGGT	20	20	45.0	50.0	58.1	57.6	3.3	0.0	0.446576	58899	59771	975-988	1.000000	79.9
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	AGAGAGAAATCCACCAGGGT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.446576	58899	59771	1007-1020	1.000000	79.8
ycf4-cemA_p4	ycf4-cemA	4	AAAAAGCTGCTGAATTGGCC	AGAGAGAAATCCACCAGGGT	20	20	45.0	50.0	57.8	57.6	1.4	0.0	0.525226	58899	59771	989-1002	1.000000	79.3
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	AGAGAGAAATCCACCAGGGT	20	20	45.0	50.0	57.8	57.6	1.4	0.0	0.571515	58899	59771	987-1000	1.000000	78.9
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	61655	62423	902-907	1.000000	79.3
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	61655	62423	892-897	1.000000	79.3
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	61655	62423	940-945	1.000000	77.5
petA-psbJ_p4	petA-psbJ	4	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	61655	62423	939-944	1.000000	77.5
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	61655	62423	930-935	1.000000	77.5
rps12-clpP_p1	rps12-clpP	1	GTACATGTTCCTCGACGCTG	GCAACAGAGGCTCAAGCTTA	20	20	55.0	50.0	58.7	58.2	2.3	18.3	0.917321	68347	68507	301-316	1.000000	77.6
rps12-clpP_p2	rps12-clpP	2	AGTACATGTTCCTCGACGCT	GCAACAGAGGCTCAAGCTTA	20	20	50.0	50.0	58.8	58.2	2.3	18.3	1.011704	68347	68507	302-317	1.000000	76.8
rps12-clpP_p3	rps12-clpP	3	TACATGTTCCTCGACGCTGA	GCAACAGAGGCTCAAGCTTA	20	20	50.0	50.0	58.8	58.2	2.3	18.3	1.016045	68347	68507	300-315	1.000000	76.8
rps12-clpP_p4	rps12-clpP	4	TAGTACATGTTCCTCGACGC	GCAACAGAGGCTCAAGCTTA	20	20	50.0	50.0	57.2	58.2	2.3	18.3	1.024873	68347	68507	303-318	1.000000	76.7
rps12-clpP_p5	rps12-clpP	5	GTACATGTTCCTCGACGCTG	GTACAAAGAACGGGCAAACC	20	20	55.0	50.0	58.7	57.6	2.3	0.0	1.141620	68347	68507	367-382	1.000000	74.9
clpP-psbB_p1	clpP-psbB	1	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.541514	70520	70969	516-517	1.000000	77.7
clpP-psbB_p2	clpP-psbB	2	TCCTCTCCAGGACTTCGAAA	AGCAACCAAAGCTGTATGCA	20	20	50.0	45.0	57.7	58.4	0.0	10.2	0.684567	70520	70969	589-590	1.000000	76.3
clpP-psbB_p3	clpP-psbB	3	TCCTCTCCAGGACTTCGAAA	ACACGATACCAAGGCAAACC	20	20	50.0	50.0	57.7	58.5	0.0	0.0	0.773898	70520	70969	519-520	1.000000	75.8
clpP-psbB_p4	clpP-psbB	4	GATGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	57.5	58.2	19.7	0.0	0.784592	70520	70969	525-526	1.000000	75.7
clpP-psbB_p5	clpP-psbB	5	TCCTCTCCAGGACTTCGAAA	TGAACACGATACCAAGGCAA	20	20	50.0	45.0	57.7	57.4	0.0	0.0	0.856821	70520	70969	522-523	1.000000	75.1
rps8-rpl14_p1	rps8-rpl14	1	ACTCGAACCACGCTTTTTCT	TGCGATTGCCAGAGAATTGA	20	20	45.0	45.0	58.1	57.9	0.0	1.5	0.162915	79021	79235	340-362	1.000000	83.4
rps8-rpl14_p2	rps8-rpl14	2	ACTCGAACCACGCTTTTTCT	GTGCGATTGCCAGAGAATTG	20	20	45.0	50.0	58.1	57.8	0.0	1.5	0.234919	79021	79235	341-363	1.000000	82.8
rps8-rpl14_p3	rps8-rpl14	3	ACTCGAACCACGCTTTTTCT	AGAGTTTTTGGTGCGATTGC	20	20	45.0	45.0	58.1	57.6	0.0	0.0	0.472811	79021	79235	351-373	1.000000	80.9
rps8-rpl14_p4	rps8-rpl14	4	ACTCGAACCACGCTTTTTCT	GAGTTTTTGGTGCGATTGCC	20	20	45.0	50.0	58.1	58.6	0.0	1.5	0.632162	79021	79235	350-372	1.000000	79.6
rps8-rpl14_p5	rps8-rpl14	5	ACTCGAACCACGCTTTTTCT	GTTTCATTAGCTCCCGAGGT	20	20	45.0	50.0	58.1	57.3	0.0	0.0	0.743801	79021	79235	297-319	1.000000	78.8
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	TCCAAATATTTTTACGAATACGCT	20	24	50.0	29.2	58.0	55.3	17.5	0.0	6.751912	110038	111051	1173-1241	1.000000	42.8
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	58.0	55.3	17.5	0.0	6.763201	110038	111051	1171-1239	1.000000	42.8
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	58.0	55.3	17.5	0.0	6.763201	110038	111051	1172-1240	1.000000	42.8
ndhF-rpl32_p4	ndhF-rpl32	4	CTTTTTGTTGCCGTCGGAAA	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	57.8	55.3	17.5	0.0	6.915793	110038	111051	1179-1247	1.000000	42.8
ndhF-rpl32_p5	ndhF-rpl32	5	TTTGTTGCCGTCGGAAAAAG	TCCAAATATTTTTACGAATACGCT	20	24	45.0	29.2	57.8	55.3	17.5	0.0	6.915793	110038	111051	1176-1244	1.000000	42.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	111217	112104	992-1003	1.000000	81.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	111217	112104	993-1004	1.000000	81.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGGCGTTAAAAGCTTTGTCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.6	57.9	13.5	0.0	0.517210	111217	112104	1002-1013	1.000000	80.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGGCGTTAAAAGCTTTGTCA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.6	57.8	13.5	0.0	0.589228	111217	112104	1001-1012	1.000000	80.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGGCGTTAAAAGCTTTGTCA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.6	57.8	13.5	0.0	0.589228	111217	112104	1000-1011	1.000000	80.2
ycf1_p1	ycf1	1	GGGGGCGATTCCACATTATT	ATCCTTTTGGTCCTCCTCGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.052002	123860	123979	424-424	1.000000	83.0
ycf1_p2	ycf1	2	GGGGGCGATTCCACATTATT	CGGTTTTGGGAATGGAAACG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.063537	123860	123979	448-448	1.000000	82.9
ycf1_p3	ycf1	3	GGGGGCGATTCCACATTATT	AACAACCAGGAAGACGCAAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023367	123860	123979	833-833	1.000000	82.1
ycf1_p4	ycf1	4	CAAAGTTTTGGGGGCGATTC	AACAACCAGGAAGACGCAAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.097592	123860	123979	842-842	1.000000	81.5
ycf1_p5	ycf1	5	AGTTGTAGAGCGATTCTGCG	AACAACCAGGAAGACGCAAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.104110	123860	123979	953-953	1.000000	81.4