primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	TACCAATGTCAACCAAGCCA	CGTGCGGTGTAATTCCATTG	20	20	45.0	50.0	57.3	57.8	0.0	0.0	0.838864	1377	3961	2754-2763	1.000000	73.5
trnK-UUU_p2	trnK-UUU	2	ACCAATGTCAACCAAGCCAG	CGTGCGGTGTAATTCCATTG	20	20	50.0	50.0	58.7	57.8	0.0	0.0	0.841379	1377	3961	2753-2762	1.000000	73.5
trnK-UUU_p3	trnK-UUU	3	ATACCAATGTCAACCAAGCCA	CGTGCGGTGTAATTCCATTG	21	20	42.9	50.0	57.8	57.8	0.0	0.0	1.340628	1377	3961	2755-2764	1.000000	69.5
trnK-UUU_p4	trnK-UUU	4	TACCAATGTCAACCAAGCCAG	CGTGCGGTGTAATTCCATTG	21	20	47.6	50.0	58.5	57.8	0.0	0.0	1.658839	1377	3961	2754-2763	1.000000	67.0
trnK-UUU_p5	trnK-UUU	5	CAATGTCAACCAAGCCAGC	CGTGCGGTGTAATTCCATTG	19	20	52.6	50.0	57.5	57.8	0.0	0.0	1.674830	1377	3961	2751-2760	1.000000	66.8
trnS-GCU-psbD_p1	trnS-GCU-psbD	1	ATTAGCAATCCGCCGCTTTA	GCCGGACCATCCTACAAAAA	20	20	45.0	50.0	58.0	58.2	0.0	5.0	0.209227	8465	9449	1128-1130	1.000000	78.7
trnS-GCU-psbD_p2	trnS-GCU-psbD	2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	20	20	45.0	45.0	58.0	58.2	0.0	0.0	0.284276	8465	9449	1115-1117	1.000000	78.1
trnS-GCU-psbD_p3	trnS-GCU-psbD	3	GCTTTAGTCCACTCAGCCAT	GCCGGACCATCCTACAAAAA	20	20	50.0	50.0	57.6	58.2	0.9	5.0	0.580969	8465	9449	1114-1116	1.000000	75.7
trnS-GCU-psbD_p4	trnS-GCU-psbD	4	ATTAGCAATCCGCCGCTTTA	GAAACGGTCCCTTCGTAACC	20	20	45.0	55.0	58.0	58.6	0.0	0.0	0.605692	8465	9449	1107-1109	1.000000	75.5
trnS-GCU-psbD_p5	trnS-GCU-psbD	5	GCTTTAGTCCACTCAGCCAT	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.6	58.2	0.9	0.0	0.656018	8465	9449	1101-1103	1.000000	75.1
psbZ-trnG-UCC_p1	psbZ-trnG-UCC	1	TTGCTTCTCCTGATGGTTGG	AGCCTTCCAAGCTAACGATG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.234861	12660	13611	1102-1106	1.000000	78.9
psbZ-trnG-UCC_p2	psbZ-trnG-UCC	2	TTGCTTCTCCTGATGGTTGG	CCTAGCCTTCCAAGCTAACG	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.263697	12660	13611	1105-1109	1.000000	78.7
psbZ-trnG-UCC_p3	psbZ-trnG-UCC	3	GCTTCTCCTGATGGTTGGTC	AGCCTTCCAAGCTAACGATG	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.285842	12660	13611	1100-1104	1.000000	78.5
psbZ-trnG-UCC_p4	psbZ-trnG-UCC	4	GCTTCTCCTGATGGTTGGTC	CCTAGCCTTCCAAGCTAACG	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.314679	12660	13611	1103-1107	1.000000	78.3
psbZ-trnG-UCC_p5	psbZ-trnG-UCC	5	TTGCTTCTCCTGATGGTTGG	CCAACGTCGACTATAACCCC	20	20	50.0	55.0	57.8	57.8	0.0	14.7	0.419409	12660	13611	1123-1127	1.000000	77.4
trnG-UCC-trnT-GGU_p1	trnG-UCC-trnT-GGU	1	ACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	21	21	38.1	52.4	55.5	58.1	0.0	0.0	4.684647	14352	16352	2129-2155	1.000000	42.9
trnG-UCC-trnT-GGU_p2	trnG-UCC-trnT-GGU	2	ACGAATCACACTTTTACCACT	GACTTACGTCTTACCATGGCA	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.776909	14352	16352	2142-2168	1.000000	42.2
trnG-UCC-trnT-GGU_p3	trnG-UCC-trnT-GGU	3	ACGAATCACACTTTTACCACT	TGACTTACGTCTTACCATGGC	21	21	38.1	47.6	55.5	57.8	0.0	13.0	4.776909	14352	16352	2143-2169	1.000000	42.2
trnG-UCC-trnT-GGU_p4	trnG-UCC-trnT-GGU	4	AGAACGAATCACACTTTTACCAC	CCATGGCATTACTCTACCACC	23	21	39.1	52.4	57.3	58.1	0.0	0.0	4.817363	14352	16352	2132-2158	1.000000	41.9
trnG-UCC-trnT-GGU_p5	trnG-UCC-trnT-GGU	5	GAACGAATCACACTTTTACCACT	CCATGGCATTACTCTACCACC	23	21	39.1	52.4	57.3	58.1	0.0	0.0	4.817363	14352	16352	2131-2157	1.000000	41.9
psbM-petN_p1	psbM-petN	1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	20	20	40.0	50.0	54.5	58.2	0.0	0.0	3.670895	18697	19468	941-998	1.000000	52.6
psbM-petN_p2	psbM-petN	2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	20	20	40.0	60.0	54.5	58.3	0.0	0.0	3.801267	18697	19468	885-942	1.000000	51.6
psbM-petN_p3	psbM-petN	3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	20	20	40.0	55.0	54.5	58.4	0.0	0.0	3.891699	18697	19468	884-941	1.000000	50.9
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	38.1	50.0	55.2	58.2	0.0	0.0	3.908487	18697	19468	916-973	1.000000	50.7
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	21	20	38.1	60.0	55.2	58.3	0.0	0.0	4.038859	18697	19468	860-917	1.000000	49.8
rpoC2_p1	rpoC2	1	GCCAGAGGAAATGCATCTCA	CCGTCATCCCATTTTGAGGA	20	20	50.0	50.0	58.0	57.6	14.4	0.0	0.466812	27683	27802	301-301	1.000000	76.8
rpoC2_p2	rpoC2	2	GCCAGAGGAAATGCATCTCA	TCCGTCATCCCATTTTGAGG	20	20	50.0	50.0	58.0	57.6	14.4	0.0	0.466812	27683	27802	302-302	1.000000	76.8
rpoC2_p3	rpoC2	3	AGCCAGAGGAAATGCATCTC	CCGTCATCCCATTTTGAGGA	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.763492	27683	27802	302-302	1.000000	74.4
rpoC2_p4	rpoC2	4	AGCCAGAGGAAATGCATCTC	TCCGTCATCCCATTTTGAGG	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.763492	27683	27802	303-303	1.000000	74.4
rpoC2_p5	rpoC2	5	GAGCCAGAGGAAATGCATCT	CCGTCATCCCATTTTGAGGA	20	20	50.0	50.0	57.7	57.6	0.0	0.0	0.763492	27683	27802	303-303	1.000000	74.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.5	57.9	0.0	0.0	0.540775	52520	53263	877-889	1.000000	76.0
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.4	57.9	0.0	0.0	0.680025	52520	53263	881-893	1.000000	74.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	57.5	58.3	0.0	22.8	0.770156	52520	53263	878-890	1.000000	74.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.8	57.9	0.0	0.0	0.875460	52520	53263	880-892	1.000000	73.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	20	20	55.0	55.0	57.4	58.3	0.0	22.8	0.909407	52520	53263	882-894	1.000000	73.1
rbcL_p1	rbcL	1	AGTAGACCCTGTCGTTGTGA	ACTGCATTTCGTAACTGCTT	20	20	50.0	40.0	58.0	56.0	0.0	9.9	2.035646	56998	58431	1565-1565	1.000000	64.2
rbcL_p2	rbcL	2	ATCGAGTAGACCCTGTCGTT	ACTGCATTTCGTAACTGCTT	20	20	50.0	40.0	57.9	56.0	0.0	9.9	2.152540	56998	58431	1569-1569	1.000000	63.3
rbcL_p3	rbcL	3	AGTAGACCCTGTCGTTGTGA	ACTGCATTTCGTAACTGCTTT	20	21	50.0	38.1	58.0	56.7	0.0	9.9	2.272339	56998	58431	1565-1565	1.000000	62.3
rbcL_p4	rbcL	4	TAGACCCTGTCGTTGTGAGA	ACTGCATTTCGTAACTGCTT	20	20	50.0	40.0	57.7	56.0	0.0	9.9	2.308306	56998	58431	1563-1563	1.000000	62.0
rbcL_p5	rbcL	5	GTAGACCCTGTCGTTGTGAG	ACTGCATTTCGTAACTGCTT	20	20	55.0	40.0	57.7	56.0	0.0	9.9	2.376064	56998	58431	1564-1564	1.000000	61.5
rbcL-psaI_p1	rbcL-psaI	1	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.2	58.0	0.0	26.9	0.195661	58432	59723	1430-1432	1.000000	79.3
rbcL-psaI_p2	rbcL-psaI	2	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.2	57.9	0.0	26.8	0.272195	58432	59723	1436-1438	1.000000	78.7
rbcL-psaI_p3	rbcL-psaI	3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.6	58.0	0.0	26.9	0.380261	58432	59723	1407-1409	1.000000	77.8
rbcL-psaI_p4	rbcL-psaI	4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.6	58.0	0.0	26.9	0.421933	58432	59723	1412-1414	1.000000	77.5
rbcL-psaI_p5	rbcL-psaI	5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.6	57.9	0.0	26.8	0.456795	58432	59723	1413-1415	1.000000	77.2
petG-trnW-CCA_p1	petG-trnW-CCA	1	TACAGACGTGGGGATCAGTT	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	0.0	2.6	0.083006	66415	66536	227-227	1.000000	82.5
petG-trnW-CCA_p2	petG-trnW-CCA	2	TACAGACGTGGGGATCAGTT	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.1	57.9	0.0	1.4	0.155928	66415	66536	191-191	1.000000	82.5
petG-trnW-CCA_p3	petG-trnW-CCA	3	GGGATTATTCGTGACTGCGT	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	58.1	58.0	0.0	2.6	0.080095	66415	66536	255-255	1.000000	82.2
petG-trnW-CCA_p4	petG-trnW-CCA	4	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	20	20	50.0	50.0	58.1	57.9	0.0	1.4	0.153017	66415	66536	219-219	1.000000	82.1
petG-trnW-CCA_p5	petG-trnW-CCA	5	ATACAGACGTGGGGATCAGT	AGCGCTCTTAGTTCAGTTCG	20	20	50.0	50.0	57.8	58.0	0.0	2.6	0.176748	66415	66536	228-228	1.000000	81.7
trnP-GGG-psaJ_p1	trnP-GGG-psaJ	1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.9	57.9	5.7	0.0	0.209515	66814	67200	495-495	1.000000	78.6
trnP-GGG-psaJ_p2	trnP-GGG-psaJ	2	CCTCGCTACTGCCCATAAAG	AAACCTGCTAAAGCCCCAAA	20	20	55.0	45.0	58.1	57.9	0.0	0.0	0.224524	66814	67200	571-571	1.000000	78.5
trnP-GGG-psaJ_p3	trnP-GGG-psaJ	3	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	58.2	57.9	5.7	0.0	0.266772	66814	67200	496-496	1.000000	78.2
trnP-GGG-psaJ_p4	trnP-GGG-psaJ	4	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	57.8	57.9	3.5	0.0	0.285929	66814	67200	503-503	1.000000	78.0
trnP-GGG-psaJ_p5	trnP-GGG-psaJ	5	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	20	20	45.0	45.0	58.4	57.9	5.7	0.0	0.504672	66814	67200	500-500	1.000000	76.3
clpP_p1	clpP	1	ATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	20	22	40.0	40.9	55.3	57.7	2.2	0.0	4.974091	70264	70914	758-758	1.000000	41.3
clpP_p2	clpP	2	AATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	21	22	38.1	40.9	56.1	57.7	2.2	0.0	5.164101	70264	70914	759-759	1.000000	41.1
clpP_p3	clpP	3	ATTTACAAGAAATCCGGCCA	GCGGATTCTTTCTTTCTCTTCC	20	22	40.0	45.5	55.3	57.4	2.2	0.0	5.303189	70264	70914	755-755	1.000000	41.1
clpP_p4	clpP	4	AAATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	22	22	36.4	40.9	56.9	57.7	2.2	0.0	5.427617	70264	70914	760-760	1.000000	41.1
clpP_p5	clpP	5	TCGCAGAAAACCTGAATTTAGG	ACTGCGGATTCTTTCTTTCTCT	22	22	40.9	40.9	57.0	57.7	14.5	0.0	5.306673	70264	70914	790-790	1.000000	41.0
petB_p1	petB	1	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.6	58.2	0.0	0.0	1.620707	73848	75311	1565-1579	1.000000	68.1
petB_p2	petB	2	GGTAGTTCGACCGTGGAATT	ACCACAACCTACCCGATATG	20	20	50.0	50.0	57.6	56.7	0.0	0.0	1.668360	73848	75311	1565-1579	1.000000	67.7
petB_p3	petB	3	GGTAGTTCGACCGTGGAATT	TGAAATACCACAACCTACCCG	20	21	50.0	47.6	57.6	57.7	0.0	0.0	1.712867	73848	75311	1571-1585	1.000000	67.3
petB_p4	petB	4	ATTCTGGTAGTTCGACCGTG	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73848	75311	1570-1584	1.000000	65.9
petB_p5	petB	5	CATTCTGGTAGTTCGACCGT	ACCACAACCTACCCGATATGA	20	21	50.0	47.6	57.4	58.2	0.0	0.0	1.894865	73848	75311	1571-1585	1.000000	65.9
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	20	24	55.0	33.3	58.1	57.3	0.0	0.0	4.844538	107415	108281	977-1007	1.000000	41.6
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107415	108281	949-979	1.000000	41.3
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107415	108281	950-980	1.000000	41.3
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107415	108281	951-981	1.000000	41.3
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	20	20	55.0	35.0	58.1	53.2	0.0	0.0	4.880333	107415	108281	952-982	1.000000	41.3
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108462	109163	774-778	1.000000	78.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.251505	108462	109163	773-777	1.000000	78.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGATCATTTTCCAGCGGCA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	58.2	57.9	1.4	0.0	0.286165	108462	109163	811-815	1.000000	78.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108462	109163	767-771	1.000000	78.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	58.1	57.8	0.0	0.0	0.312231	108462	109163	766-770	1.000000	78.2
ndhG_p1	ndhG	1	TGTTGGCAGTAGATGGGTTT	TCCCTTTTTCCTTCCTTGAATCT	20	23	45.0	39.1	57.0	57.7	0.0	0.0	4.309244	113349	113879	710-721	1.000000	46.8
ndhG_p2	ndhG	2	TTGTTGGCAGTAGATGGGTT	TCCCTTTTTCCTTCCTTGAATCT	20	23	45.0	39.1	57.0	57.7	0.0	0.0	4.309244	113349	113879	711-722	1.000000	46.8
ndhG_p3	ndhG	3	TTTGTTGGCAGTAGATGGGT	TCCCTTTTTCCTTCCTTGAATCT	20	23	45.0	39.1	57.0	57.7	0.0	0.0	4.309244	113349	113879	712-723	1.000000	46.8
ndhG_p4	ndhG	4	TGTTGGCAGTAGATGGGTTT	TCCTAATCCCTTTTTCCTTCCT	20	22	45.0	40.9	57.0	56.4	0.0	0.0	4.560016	113349	113879	716-727	1.000000	44.7
ndhG_p5	ndhG	5	TTGTTGGCAGTAGATGGGTT	TCCTAATCCCTTTTTCCTTCCT	20	22	45.0	40.9	57.0	56.4	0.0	0.0	4.560016	113349	113879	717-728	1.000000	44.7
ndhG-ndhI_p1	ndhG-ndhI	1	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCA	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113880	114125	483-483	1.000000	68.8
ndhG-ndhI_p2	ndhG-ndhI	2	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCC	20	20	50.0	50.0	58.2	56.7	0.0	1.1	1.591617	113880	114125	484-484	1.000000	68.8
ndhG-ndhI_p3	ndhG-ndhI	3	CCCAGCGAAAAGGCAGAATA	TGAGTCGGTTACCAATCTCCA	20	21	50.0	47.6	58.2	58.5	0.0	1.1	1.718036	113880	114125	484-484	1.000000	67.7
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAGCGAAAAGGCAGAATA	TTGAGTCGGTTACCAATCTCC	20	21	50.0	47.6	58.2	57.4	0.0	1.1	1.854682	113880	114125	485-485	1.000000	66.6
ndhG-ndhI_p5	ndhG-ndhI	5	CCCAGCGAAAAGGCAGAATA	GAGTCGGTTACCAATCTCCAT	20	21	50.0	47.6	58.2	57.2	0.0	1.1	2.071228	113880	114125	483-483	1.000000	64.9