primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnQ-UUG_p1	matK-trnQ-UUG	1	AGACCATCCAGTAGCCTGTT	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129816	6188	6307	766-766	0.500000	59.0
matK-trnQ-UUG_p2	matK-trnQ-UUG	2	TCTCGGGGCCTTCTTTTCTA	TCTATCCCAATGAGCCGTCT	20	20	50.0	50.0	58.1	57.9	0.8	0.0	0.141120	6188	6307	851-851	0.500000	58.9
matK-trnQ-UUG_p3	matK-trnQ-UUG	3	AGACCATCCAGTAGCCTGTT	TCTGGGGTCCCCTGATAATC	20	20	50.0	55.0	58.0	57.9	0.0	28.4	0.160115	6188	6307	393-393	0.500000	58.7
matK-trnQ-UUG_p4	matK-trnQ-UUG	4	AGACCATCCAGTAGCCTGTT	AGCCGTCTATCGAATCGTTG	20	20	50.0	50.0	58.0	57.9	0.0	1.5	0.162828	6188	6307	754-754	0.500000	58.7
matK-trnQ-UUG_p5	matK-trnQ-UUG	5	TCTCGGGGCCTTCTTTTCTA	TCTGGGGTCCCCTGATAATC	20	20	50.0	55.0	58.1	57.9	0.8	28.4	0.171419	6188	6307	478-478	0.500000	58.6
atpH-atpI_p1	atpH-atpI	1	CAGCAGTCCCTTGACCAATT	TGCTTTGGCTTTACTCACGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.123066	15536	16762	1529-1652	1.000000	91.0
atpH-atpI_p2	atpH-atpI	2	AACGGAAGCAGCAGAAATCA	TGCTTTGGCTTTACTCACGT	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.141987	15536	16762	1465-1588	1.000000	90.9
atpH-atpI_p3	atpH-atpI	3	CAGCAGTCCCTTGACCAATT	CAAACTATTCCGACTGGGGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.123246	15536	16762	1882-1882	0.500000	59.0
atpH-atpI_p4	atpH-atpI	4	CAGCAGTCCCTTGACCAATT	GAGAAGAATACGGCCCTTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.129932	15536	16762	1803-1803	0.500000	59.0
atpH-atpI_p5	atpH-atpI	5	CAGCAGTCCCTTGACCAATT	GGAGAAGAATACGGCCCTTG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.129932	15536	16762	1804-1804	0.500000	59.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.0	58.5	0.0	5.4	1.505426	32391	32995	707-730	1.000000	73.9
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	57.9	58.5	0.0	5.4	1.574673	32391	32995	711-734	1.000000	73.3
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGGCGGTAGGAACTAGAATGA	GTTCAATTGGTCAGAGCACC	21	20	47.6	50.0	58.0	57.0	0.0	5.4	2.025580	32391	32995	708-731	1.000000	69.7
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	GGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.4	58.5	0.0	5.4	2.039547	32391	32995	706-729	1.000000	69.6
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGCGGTAGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.4	58.5	0.0	5.4	2.049273	32391	32995	707-730	1.000000	69.5
trnS-GGA-trnT-UGU_p1	trnS-GGA-trnT-UGU	1	GCTTCTTCCCTTAGGCTGTC	GTTCTGCACGATTGGCTCTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.041403	47291	48530	2903-2903	0.500000	59.7
trnS-GGA-trnT-UGU_p2	trnS-GGA-trnT-UGU	2	AGGTGTAGTGCTTCTTCCCT	GTTCTGCACGATTGGCTCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041640	47291	48530	2912-2912	0.500000	59.7
trnS-GGA-trnT-UGU_p3	trnS-GGA-trnT-UGU	3	ACTTCACCTTCAACAAGCGT	GTTCTGCACGATTGGCTCTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.048532	47291	48530	2736-2736	0.500000	59.6
trnS-GGA-trnT-UGU_p4	trnS-GGA-trnT-UGU	4	TTCTTCCCTTAGGCTGTCCA	GTTCTGCACGATTGGCTCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049008	47291	48530	2901-2901	0.500000	59.6
trnS-GGA-trnT-UGU_p5	trnS-GGA-trnT-UGU	5	AGGCTTTTGTTTACCGAGGG	GGAACCGCTAACCAAAAAGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.154688	47291	48530	2754-2754	0.500000	58.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	48604	49378	819-836	1.000000	85.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	48604	49378	851-868	1.000000	84.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	48604	49378	813-830	1.000000	84.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AAAATAGGAAAAGGCCGGCT	CCGTAGCGTCTACCGATTTC	20	20	45.0	55.0	57.8	58.2	28.6	10.2	0.480165	48604	49378	874-891	1.000000	84.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	48604	49378	851-868	1.000000	83.2
atpB-rbcL_p1	atpB-rbcL	1	CGACCTTTCACTACCAGAGC	AGGTTGAGGAGTGACTCGAA	20	20	55.0	50.0	58.0	58.0	0.0	6.5	0.005533	56891	57724	1098-1166	1.000000	91.2
atpB-rbcL_p2	atpB-rbcL	2	GACCTTTCACTACCAGAGCG	AGGTTGAGGAGTGACTCGAA	20	20	55.0	50.0	58.0	58.0	0.0	6.5	0.005533	56891	57724	1097-1165	1.000000	91.2
atpB-rbcL_p3	atpB-rbcL	3	GACCTTTCACTACCAGAGCG	AGAAACGATCTCTCCAACGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.004662	56891	57724	1614-1682	1.000000	88.7
atpB-rbcL_p4	atpB-rbcL	4	CGACCTTTCACTACCAGAGC	AGAAACGATCTCTCCAACGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.004662	56891	57724	1615-1683	1.000000	88.6
atpB-rbcL_p5	atpB-rbcL	5	GACCTTTCACTACCAGAGCG	GGGGGCCTTGGAAAGTTTTA	20	20	55.0	50.0	58.0	58.0	0.0	0.8	0.020686	56891	57724	1481-1481	0.500000	59.8
rbcL-psaI_p1	rbcL-psaI	1	TGAAGGACGTGATCTTGCTG	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.188147	59204	61895	2932-2932	0.500000	58.5
rbcL-psaI_p2	rbcL-psaI	2	TGCCAAATGGAGTCCTGAAC	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	58.1	57.9	11.3	0.0	0.193837	59204	61895	2884-2884	0.500000	58.4
rbcL-psaI_p3	rbcL-psaI	3	TTTGCGGCAGTGGATACTTT	AAAGAAGCCATTGCCATTGC	20	20	45.0	45.0	58.1	57.9	1.7	0.0	0.199250	59204	61895	2823-2823	0.500000	58.4
rbcL-psaI_p4	rbcL-psaI	4	TTTGCGGCAGTGGATACTTT	TTCGCCGGAAATCACATCTT	20	20	45.0	45.0	58.1	57.9	1.7	5.0	0.203663	59204	61895	2966-2966	0.500000	58.4
rbcL-psaI_p5	rbcL-psaI	5	CCGCTTGTGAGGTATGGAAA	AAAGAAGCCATTGCCATTGC	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.211487	59204	61895	2858-2858	0.500000	58.3
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTTCGCGT	ACAAAAGAGGAGTGAATGCT	20	20	45.0	40.0	58.2	54.9	5.8	0.0	3.322496	63105	64044	954-1013	1.000000	65.4
ycf4-cemA_p2	ycf4-cemA	2	GAACAAAAAGCCGCGGAATT	ACAAAAGAGGAGTGAATGCT	20	20	45.0	40.0	58.2	54.9	33.5	0.0	3.360236	63105	64044	972-1031	1.000000	65.0
ycf4-cemA_p3	ycf4-cemA	3	CGCGTACCAATTGAAGTCCT	ACAAAAGAGGAGTGAATGCT	20	20	50.0	40.0	58.3	54.9	7.1	0.0	3.416559	63105	64044	939-998	1.000000	64.7
ycf4-cemA_p4	ycf4-cemA	4	TCGCGTACCAATTGAAGTCC	ACAAAAGAGGAGTGAATGCT	20	20	50.0	40.0	58.3	54.9	7.1	0.0	3.421376	63105	64044	940-999	1.000000	64.6
ycf4-cemA_p5	ycf4-cemA	5	TTGGCCTATTTCTTTCGCGT	ACAAAAGAGGAGTGAATGCTT	20	21	45.0	38.1	58.2	55.7	5.8	0.0	3.490391	63105	64044	954-1013	1.000000	64.1
petA-psbJ_p1	petA-psbJ	1	TCCGTGTCCAAGGTCTTTTG	AACAGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.0	7.1	0.042830	65906	66978	1771-1773	1.000000	87.8
petA-psbJ_p2	petA-psbJ	2	TCCGTGTCCAAGGTCTTTTG	ACAGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.042830	65906	66978	1770-1772	1.000000	87.8
petA-psbJ_p3	petA-psbJ	3	TCCGATCGTATTTCTCCCGA	AACAGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	15.1	7.1	0.033143	65906	66978	2261-2263	1.000000	86.5
petA-psbJ_p4	petA-psbJ	4	TCCGATCGTATTTCTCCCGA	ACAGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	15.1	0.0	0.033143	65906	66978	2260-2262	1.000000	86.5
petA-psbJ_p5	petA-psbJ	5	TCCGATCGTATTTCTCCCGA	TGCGGGTTGGTTATTTGTCA	20	20	50.0	45.0	58.0	58.0	15.1	0.0	0.045859	65906	66978	2363-2365	1.000000	86.1
rpl20-rps12_p1	rpl20-rps12	1	CTATCTCGATCGGCGGAAAC	CAGCGTCGAGGAAGATGTAC	20	20	55.0	55.0	58.3	58.2	16.4	9.8	0.483541	71789	72842	1038-1107	1.000000	88.1
rpl20-rps12_p2	rpl20-rps12	2	CTATCTCGATCGGCGGAAAC	AGCGTCGAGGAAGATGTACT	20	20	55.0	50.0	58.3	58.2	16.4	9.8	0.559689	71789	72842	1037-1106	1.000000	87.5
rpl20-rps12_p3	rpl20-rps12	3	CTATCTCGATCGGCGGAAAC	TCAGCGTCGAGGAAGATGTA	20	20	55.0	50.0	58.3	58.3	16.4	9.8	0.565902	71789	72842	1039-1108	1.000000	87.5
rpl20-rps12_p4	rpl20-rps12	4	TTATGCCTATCTCGATCGGC	CAGCGTCGAGGAAGATGTAC	20	20	50.0	55.0	57.1	58.2	16.4	9.8	1.112621	71789	72842	1044-1113	1.000000	83.1
rpl20-rps12_p5	rpl20-rps12	5	TTATGCCTATCTCGATCGGC	AGCGTCGAGGAAGATGTACT	20	20	50.0	50.0	57.1	58.2	16.4	9.8	1.188770	71789	72842	1043-1112	1.000000	82.5
ycf1_p1	ycf1	1	GAGCTACTGAGGAACAACGG	TCTCCTTCTGGCGATAGAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.014037	112357	113652	1938-1938	0.500000	59.9
ycf1_p2	ycf1	2	CCCGGAACTTCTTCGTAGTG	TCTCCTTCTGGCGATAGAGG	20	20	55.0	55.0	58.0	58.0	0.7	0.0	0.019537	112357	113652	1843-1843	0.500000	59.8
ycf1_p3	ycf1	3	CCGGAACTTCTTCGTAGTGG	TCTCCTTCTGGCGATAGAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.019537	112357	113652	1842-1842	0.500000	59.8
ycf1_p4	ycf1	4	GAGCTACTGAGGAACAACGG	GGGGACGCGTTTCTTCTTAT	20	20	55.0	50.0	58.0	58.0	0.0	15.5	0.022279	112357	113652	2353-2353	0.500000	59.8
ycf1_p5	ycf1	5	CCGGAACTTCTTCGTAGTGG	GGGGACGCGTTTCTTCTTAT	20	20	55.0	50.0	58.0	58.0	0.0	15.5	0.027778	112357	113652	2257-2257	0.500000	59.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTGGGCAGCGTTAA	TAGGAGATGACATGCCGCTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028964	116923	117814	1065-1089	1.000000	90.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GCCCACATACGACGAAGATT	TAGGAGATGACATGCCGCTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.068691	116923	117814	1689-1693	1.000000	89.9
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GCGCTATTTTGCCGAGTTTT	TAGGAGATGACATGCCGCTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.027435	116923	117814	1323-1323	0.500000	59.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGCTATTTTGCCGAGTTTT	ACCTAAAAGAGCCGCATAGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.067885	116923	117814	1850-1850	0.500000	59.5
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGCTATTTTGCCGAGTTTT	TTTAGGAGATGACATGCCGC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.069253	116923	117814	1325-1325	0.500000	59.4
ccsA-psaC_p1	ccsA-psaC	1	TGGATATGCTATTTTGGGGTCA	AAGAGATGTGAATCCGCCTG	22	20	40.9	50.0	57.2	57.7	0.0	0.0	3.127495	118940	120921	2141-2159	1.000000	66.9
ccsA-psaC_p2	ccsA-psaC	2	TGGATATGCTATTTTGGGGTCA	GCCCAACGGATTTCTTGAGT	22	20	40.9	50.0	57.2	58.5	0.0	0.0	3.270411	118940	120921	2122-2140	1.000000	65.8
ccsA-psaC_p3	ccsA-psaC	3	TGGATATGCTATTTTGGGGTCA	CTGCCCAACGGATTTCTTGA	22	20	40.9	50.0	57.2	58.5	0.0	0.0	3.271515	118940	120921	2124-2142	1.000000	65.8
ccsA-psaC_p4	ccsA-psaC	4	TGGATATGCTATTTTGGGGTCA	TGCCCAACGGATTTCTTGAG	22	20	40.9	50.0	57.2	58.5	0.0	0.0	3.271515	118940	120921	2123-2141	1.000000	65.8
ccsA-psaC_p5	ccsA-psaC	5	TGGATATGCTATTTTGGGGTCA	CGAGGACTGTGTTGGTTGTA	22	20	40.9	50.0	57.2	57.5	0.0	0.0	3.324516	118940	120921	2160-2178	1.000000	65.3
rrn16-rps12_p1	rrn16-rps12	1	CCGACTTGCATGTGTTAAGC	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.8	57.7	10.4	16.5	0.505002	142764	144680	1966-2026	1.000000	82.8
rrn16-rps12_p2	rrn16-rps12	2	GCAGGTTCTTACGCGTTACT	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.3	57.7	20.8	16.5	0.551248	142764	144680	2019-2079	1.000000	82.4
rrn16-rps12_p3	rrn16-rps12	3	TTCTTACGCGTTACTCACCC	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	20.8	16.5	0.610194	142764	144680	2014-2074	1.000000	81.9
rrn16-rps12_p4	rrn16-rps12	4	CCAGGATCGAACTCTCCATG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.5	57.7	5.6	16.5	0.780526	142764	144680	1923-1983	1.000000	80.7
rrn16-rps12_p5	rrn16-rps12	5	CGTCCGACTTGCATGTGTTA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	58.6	57.7	10.4	16.5	0.833288	142764	144680	1969-2029	1.000000	80.2