primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf21-pgmA_p1	ycf21-pgmA	1	GCGCACGTCTAATAGCTTCT	TACATCAGACCACGGCAATG	20	20	50.0	50.0	58.2	58.0	0.3	0.0	0.181315	39543	40023	1028-1037	0.285714	58.7
ycf21-pgmA_p2	ycf21-pgmA	2	CACTGGTCATGGATTGCTGA	CATTACATCAGACCACGGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.141078	39543	40023	1295-1295	0.142857	44.6
ycf21-pgmA_p3	ycf21-pgmA	3	CACTGGTCATGGATTGCTGA	TACATCAGACCACGGCAATG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.141078	39543	40023	1292-1292	0.142857	44.6
ycf21-pgmA_p4	ycf21-pgmA	4	TCACTGGTCATGGATTGCTG	CATTACATCAGACCACGGCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.141078	39543	40023	1296-1296	0.142857	44.6
ycf21-pgmA_p5	ycf21-pgmA	5	TCACTGGTCATGGATTGCTG	TACATCAGACCACGGCAATG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.141078	39543	40023	1293-1293	0.142857	44.6
apcF-trnR(ccg)_p1	apcF-trnR(ccg)	1	CTTGCTGCCGCTTCTTTAAC	ATCCACTGAGTTACATGCCC	20	20	50.0	50.0	58.0	57.3	0.7	0.0	0.748536	55219	55694	669-669	0.142857	39.7
apcF-trnR(ccg)_p2	apcF-trnR(ccg)	2	TGATTTTAGCGCAGGCGTAT	ATCCACTGAGTTACATGCCC	20	20	45.0	50.0	58.1	57.3	11.5	0.0	0.769535	55219	55694	949-949	0.142857	39.6
apcF-trnR(ccg)_p3	apcF-trnR(ccg)	3	TCTTGCTGCCGCTTCTTTAA	ATCCACTGAGTTACATGCCC	20	20	45.0	50.0	58.1	57.3	0.7	0.0	0.826763	55219	55694	670-670	0.142857	39.1
apcF-trnR(ccg)_p4	apcF-trnR(ccg)	4	ATCTTGCTGCCGCTTCTTTA	ATCCACTGAGTTACATGCCC	20	20	45.0	50.0	57.9	57.3	0.7	0.0	0.831499	55219	55694	671-671	0.142857	39.1
apcF-trnR(ccg)_p5	apcF-trnR(ccg)	5	TAATCTTGCTGCCGCTTCTT	ATCCACTGAGTTACATGCCC	20	20	45.0	50.0	57.9	57.3	0.7	0.0	0.831499	55219	55694	673-673	0.142857	39.1
ssrA_p1	ssrA	1	AGGGCATGTAACTCAGTGGA	CACTGATACCTGACATGGCA	20	20	50.0	50.0	58.3	57.3	0.0	0.0	1.040402	55811	56205	841-841	0.142857	37.4
ssrA_p2	ssrA	2	AAGGGCATGTAACTCAGTGG	CACTGATACCTGACATGGCA	20	20	50.0	50.0	57.5	57.3	0.0	0.0	1.193888	55811	56205	842-842	0.142857	36.2
ssrA_p3	ssrA	3	AGGGCATGTAACTCAGTGGA	TCACTGATACCTGACATGGC	20	20	50.0	50.0	58.3	57.0	0.0	0.0	1.331423	55811	56205	842-842	0.142857	35.1
ssrA_p4	ssrA	4	AGGGCATGTAACTCAGTGGA	CACTGATACCTGACATGGCAA	20	21	50.0	47.6	58.3	58.0	0.0	1.1	1.353504	55811	56205	841-841	0.142857	34.9
ssrA_p5	ssrA	5	GGGCATGTAACTCAGTGGAT	CACTGATACCTGACATGGCA	20	20	50.0	50.0	57.3	57.3	0.0	0.0	1.414628	55811	56205	840-840	0.142857	34.4
ycf80_p1	ycf80	1	AGGGCATGTAACTCAGTGGA	CGTTATCGAGGACCACGTTT	20	20	50.0	50.0	58.3	58.0	0.0	11.2	0.356715	56414	57868	2741-2879	1.000000	89.1
ycf80_p2	ycf80	2	AAGGGCATGTAACTCAGTGG	CGTTATCGAGGACCACGTTT	20	20	50.0	50.0	57.5	58.0	0.0	11.2	0.510201	56414	57868	2742-2842	0.857143	82.2
ycf80_p3	ycf80	3	AGGGCATGTAACTCAGTGGA	GCACCTACTATACCTGCAGC	20	20	50.0	55.0	58.3	57.8	0.0	8.7	0.499756	56414	57868	2527-2582	0.571429	69.1
ycf80_p4	ycf80	4	AGGGCATGTAACTCAGTGGA	TGGCACCTACTATACCTGCA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.511871	56414	57868	2529-2584	0.571429	69.0
ycf80_p5	ycf80	5	AGGGCATGTAACTCAGTGGA	GTACAAGGTGCAACAGGTCT	20	20	50.0	50.0	58.3	57.7	0.0	3.6	0.599530	56414	57868	2608-2660	0.571429	68.3
trnMe(cat)_p1	trnMe(cat)	1	TTCCAAGCTAATGATGCGGG	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.574253	66168	67658	2122-2387	0.714286	76.0
trnMe(cat)_p2	trnMe(cat)	2	GCTAATGATGCGGGTTCGAT	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	58.5	57.7	0.0	0.0	0.740466	66168	67658	2116-2381	0.714286	74.6
trnMe(cat)_p3	trnMe(cat)	3	CCAAGCTAATGATGCGGGTT	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	58.6	57.7	0.0	0.0	0.860803	66168	67658	2120-2385	0.714286	73.7
trnMe(cat)_p4	trnMe(cat)	4	AATGATGCGGGTTCGATTCC	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	58.7	57.7	0.0	0.0	0.944394	66168	67658	2113-2378	0.714286	73.0
trnMe(cat)_p5	trnMe(cat)	5	AAGCTAATGATGCGGGTTCG	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	58.7	57.7	0.0	0.0	0.953851	66168	67658	2118-2383	0.714286	72.9
orf244_p1	orf244	1	TATGCATCTGGCTGGATTCG	AAAATGCAGACGTATTGCGC	20	20	50.0	45.0	57.8	58.1	0.2	16.8	0.279312	66324	67058	955-1185	0.857143	83.5
orf244_p2	orf244	2	TATGCATCTGGCTGGATTCG	CTACTAAGCATCTGTGGCCG	20	20	50.0	55.0	57.8	58.1	0.2	0.0	0.316272	66324	67058	1499-1627	0.285714	56.9
orf244_p3	orf244	3	ACCAGCGACGTCCTTTTAAG	AAAATGCAGACGTATTGCGC	20	20	50.0	45.0	58.2	58.1	10.6	16.8	0.312943	66324	67058	934-934	0.142857	43.2
orf244_p4	orf244	4	GAACCAGCGACGTCCTTTTA	AAAATGCAGACGTATTGCGC	20	20	50.0	45.0	58.2	58.1	10.6	16.8	0.317936	66324	67058	936-936	0.142857	43.2
orf244_p5	orf244	5	AACCAGCGACGTCCTTTTAA	AAAATGCAGACGTATTGCGC	20	20	45.0	45.0	57.8	58.1	10.6	16.8	0.348551	66324	67058	935-935	0.142857	42.9
orf244-trnMe(cat)_p1	orf244-trnMe(cat)	1	AGAGGGCAACGACTGAATTC	CTACTAAGCATCTGTGGCCG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.242513	67059	67620	1159-1159	0.142857	43.8
orf244-trnMe(cat)_p2	orf244-trnMe(cat)	2	GAGGGCAACGACTGAATTCT	CTACTAAGCATCTGTGGCCG	20	20	50.0	55.0	57.9	58.1	7.1	0.0	0.242513	67059	67620	1158-1158	0.142857	43.8
orf244-trnMe(cat)_p3	orf244-trnMe(cat)	3	AGAGGGCAACGACTGAATTC	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.353997	67059	67620	1761-1761	0.142857	42.9
orf244-trnMe(cat)_p4	orf244-trnMe(cat)	4	GAGGGCAACGACTGAATTCT	CAGGAAGAACGAGCATTTGC	20	20	50.0	50.0	57.9	57.7	7.1	0.0	0.353997	67059	67620	1760-1760	0.142857	42.9
orf244-trnMe(cat)_p5	orf244-trnMe(cat)	5	GAGGGCAACGACTGAATTCT	TACTAAGCATCTGTGGCCGA	20	20	50.0	50.0	57.9	58.5	7.1	0.0	0.619815	67059	67620	1157-1157	0.142857	40.8
orf692_p1	orf692	1	CGGTTTGATAACGCGATACC	AACGACCCGTCAAGAAATCC	20	20	50.0	50.0	57.1	58.2	15.9	0.0	1.112134	68643	70721	2867-2884	0.428571	56.2
orf692_p2	orf692	2	CGGTTTGATAACGCGATACC	CGACCCGTCAAGAAATCCAA	20	20	50.0	50.0	57.1	58.2	15.9	0.0	1.113401	68643	70721	2865-2882	0.428571	56.2
orf692_p3	orf692	3	TGTTACGGTTTGATAACGCG	AACGACCCGTCAAGAAATCC	20	20	45.0	50.0	56.9	58.2	3.5	0.0	1.332923	68643	70721	2872-2889	0.428571	54.5
orf692_p4	orf692	4	TGTTACGGTTTGATAACGCG	CGACCCGTCAAGAAATCCAA	20	20	45.0	50.0	56.9	58.2	3.5	0.0	1.334190	68643	70721	2870-2887	0.428571	54.5
orf692_p5	orf692	5	TGTTACGGTTTGATAACGCGA	AACGACCCGTCAAGAAATCC	21	20	42.9	50.0	58.3	58.2	15.9	0.0	1.524920	68643	70721	2872-2889	0.428571	52.9
trnL(caa)-sufB_p1	trnL(caa)-sufB	1	TGCGTATACCAATTTCGCCA	TACCCCATGTTGGTTCTTGC	20	20	45.0	50.0	58.0	58.1	0.0	9.2	0.107717	72849	73690	1099-1125	0.857143	83.9
trnL(caa)-sufB_p2	trnL(caa)-sufB	2	ACCAATTTCGCCATCTACCC	TACCCCATGTTGGTTCTTGC	20	20	50.0	50.0	57.9	58.1	0.0	9.2	0.134910	72849	73690	1092-1118	0.857143	83.7
trnL(caa)-sufB_p3	trnL(caa)-sufB	3	TGCGTATACCAATTTCGCCA	TAATTCGAGTGGACAGCGTG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.034789	72849	73690	1505-1505	0.142857	45.4
trnL(caa)-sufB_p4	trnL(caa)-sufB	4	ACCAATTTCGCCATCTACCC	TAATTCGAGTGGACAGCGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.061983	72849	73690	1498-1498	0.142857	45.2
trnL(caa)-sufB_p5	trnL(caa)-sufB	5	GAAGGTCTAGCAGACGAAGC	TAATTCGAGTGGACAGCGTG	20	20	55.0	50.0	58.1	58.0	9.3	0.0	0.093249	72849	73690	1820-1820	0.142857	45.0
acpP-ycf45_p1	acpP-ycf45	1	GGAGAGGTGGTAGAGTTGGT	CGAAACGTAGAGGGTCACAA	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.487252	112648	112938	1350-1356	0.428571	63.4
acpP-ycf45_p2	acpP-ycf45	2	GGAGAGGTGGTAGAGTTGGT	ATGAAGCGAAACGTAGAGGG	20	20	55.0	50.0	58.1	57.7	0.0	0.0	0.355273	112648	112938	1356-1362	0.285714	58.7
acpP-ycf45_p3	acpP-ycf45	3	AAATCCTGGTCGTGAAGGTG	ATGAAGCGAAACGTAGAGGG	20	20	50.0	50.0	57.8	57.7	0.0	0.0	0.480257	112648	112938	1672-1689	0.285714	58.5
acpP-ycf45_p4	acpP-ycf45	4	GGAGAGGTGGTAGAGTTGGT	GAAGCGAAACGTAGAGGGTC	20	20	55.0	55.0	58.1	58.4	0.0	0.0	0.433273	112648	112938	1354-1360	0.285714	58.1
acpP-ycf45_p5	acpP-ycf45	5	CACCCTCTCCGTCCTTCATA	ATGAAGCGAAACGTAGAGGG	20	20	55.0	50.0	58.2	57.7	0.0	0.0	0.518591	112648	112938	1287-1287	0.142857	41.6
dnaB_p1	dnaB	1	TCAGTCCACTGCTCTACCAA	CAGACATTGCAACTCCTGGT	20	20	50.0	50.0	58.0	58.1	1.5	0.0	0.108091	151249	153054	2410-2510	0.571429	73.6
dnaB_p2	dnaB	2	TCCACTGCTCTACCAACTGA	CAGACATTGCAACTCCTGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.108091	151249	153054	2406-2506	0.571429	73.6
dnaB_p3	dnaB	3	TTCAGTCCACTGCTCTACCA	CAGACATTGCAACTCCTGGT	20	20	50.0	50.0	58.0	58.1	1.5	0.0	0.108091	151249	153054	2411-2511	0.571429	73.6
dnaB_p4	dnaB	4	AGTCCACTGCTCTACCAACT	CAGACATTGCAACTCCTGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.115314	151249	153054	2408-2508	0.571429	73.5
dnaB_p5	dnaB	5	TTTGAACTGGTGACACGAGG	CAGACATTGCAACTCCTGGT	20	20	50.0	50.0	58.1	58.1	20.1	0.0	0.150305	151249	153054	2434-2534	0.571429	73.2
ntcA_p1	ntcA	1	CTGCCCAGTAGCCAATTTGA	TCCCGTCATTCGCCCTTATA	20	20	50.0	50.0	58.2	58.3	0.0	0.0	0.454782	158174	158836	1884-1887	0.571429	67.3
ntcA_p2	ntcA	2	GGTACTGCTTCAGGAACACC	TCCCGTCATTCGCCCTTATA	20	20	55.0	50.0	58.2	58.3	0.0	0.0	0.483210	158174	158836	1918-1921	0.571429	67.0
ntcA_p3	ntcA	3	GGTGGTACGTACTTGCTTGT	TCCCGTCATTCGCCCTTATA	20	20	50.0	50.0	57.8	58.3	24.1	0.0	0.452221	158174	158836	1525-1528	0.428571	61.5
ntcA_p4	ntcA	4	GGCAGACCATCGGTGAATAG	TCCCGTCATTCGCCCTTATA	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.421814	158174	158836	1712-1712	0.142857	42.3
ntcA_p5	ntcA	5	GGCAGACCATCGGTGAATAG	CCCGTCATTCGCCCTTATAA	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.665681	158174	158836	1711-1711	0.142857	40.4
rps1-syfB_p1	rps1-syfB	1	ACAGGCTGTTGGAAGACCTA	TGGTCAGAAGATGGACAAGA	20	20	50.0	45.0	58.3	55.8	15.9	0.0	2.488961	160666	161109	1221-1221	0.142857	25.8
rps1-syfB_p2	rps1-syfB	2	ACAGGCTGTTGGAAGACCTA	TGGTCAGAAGATGGACAAGAA	20	21	50.0	42.9	58.3	56.6	15.9	0.0	2.696019	160666	161109	1221-1221	0.142857	24.1
rps1-syfB_p3	rps1-syfB	3	TAACAGGCTGTTGGAAGACC	TGGTCAGAAGATGGACAAGA	20	20	50.0	45.0	57.4	55.8	36.4	0.0	2.780025	160666	161109	1223-1223	0.142857	23.5
rps1-syfB_p4	rps1-syfB	4	ACAGGCTGTTGGAAGACCTA	ATGGTCAGAAGATGGACAAGA	20	21	50.0	42.9	58.3	56.4	15.9	0.0	2.919810	160666	161109	1222-1222	0.142857	22.4
rps1-syfB_p5	rps1-syfB	5	ACAGGCTGTTGGAAGACCTA	TGGTCAGAAGATGGACAAGAAA	20	22	50.0	40.9	58.3	57.3	15.9	0.0	2.975523	160666	161109	1221-1221	0.142857	21.9
syfB_p1	syfB	1	TTGAGGAAAGTCCGGTCTCT	CTCGAAGATGAAGAAGGGGC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.064377	161110	163260	2751-2751	0.142857	45.2
syfB_p2	syfB	2	GGTGCATTAAGAGCGTACCA	CTCGAAGATGAAGAAGGGGC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.071797	161110	163260	2636-2636	0.142857	45.1
syfB_p3	syfB	3	GTGCCACAGAAATAAACCGC	CTCGAAGATGAAGAAGGGGC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.076067	161110	163260	2680-2680	0.142857	45.1
syfB_p4	syfB	4	TTGAGGAAAGTCCGGTCTCT	CGAAGATGAAGAAGGGGCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.124983	161110	163260	2749-2749	0.142857	44.7
syfB_p5	syfB	5	GGTGCATTAAGAGCGTACCA	CGAAGATGAAGAAGGGGCAA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.132402	161110	163260	2634-2634	0.142857	44.7
trnR(acg)-groEL_p1	trnR(acg)-groEL	1	TGAACATTCCAGTGCGTTCA	GATTTGTACCAGCCGCTACA	20	20	45.0	50.0	58.0	58.0	10.0	0.0	0.058125	165325	165373	976-976	0.142857	45.2
trnR(acg)-groEL_p2	trnR(acg)-groEL	2	GATCCACTGAGCTACAAGCC	GATTTGTACCAGCCGCTACA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.071797	165325	165373	404-404	0.142857	45.1
trnR(acg)-groEL_p3	trnR(acg)-groEL	3	ATACTTGCTGTGCCTTCTGG	GATTTGTACCAGCCGCTACA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.138343	165325	165373	762-762	0.142857	44.6
trnR(acg)-groEL_p4	trnR(acg)-groEL	4	TTTAAATCGGGCAAGGAGGG	GATTTGTACCAGCCGCTACA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.156352	165325	165373	464-464	0.142857	44.5
trnR(acg)-groEL_p5	trnR(acg)-groEL	5	TGTGCCTTCTGGTCTCAATG	GATTTGTACCAGCCGCTACA	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.208136	165325	165373	754-754	0.142857	44.0
groEL-ycf65_p1	groEL-ycf65	1	AAAGGTTACCCGTTCTGCTG	GGTGCACAAATGACACCAAA	20	20	50.0	45.0	58.1	57.4	0.0	20.4	0.701137	166958	167499	985-1005	0.428571	61.2
groEL-ycf65_p2	groEL-ycf65	2	AGCAAAGGTTACCCGTTCTG	GGTGCACAAATGACACCAAA	20	20	50.0	45.0	58.1	57.4	0.0	20.4	0.701137	166958	167499	988-1008	0.428571	61.2
groEL-ycf65_p3	groEL-ycf65	3	CAAAGGTTACCCGTTCTGCT	GGTGCACAAATGACACCAAA	20	20	50.0	45.0	58.1	57.4	0.0	20.4	0.701137	166958	167499	986-1006	0.428571	61.2
groEL-ycf65_p4	groEL-ycf65	4	CAGCAAAGGTTACCCGTTCT	GGTGCACAAATGACACCAAA	20	20	50.0	45.0	58.1	57.4	0.0	20.4	0.701137	166958	167499	989-1009	0.428571	61.2
groEL-ycf65_p5	groEL-ycf65	5	GTTACCCGTTCTGCTGTTCA	GGTGCACAAATGACACCAAA	20	20	50.0	45.0	58.1	57.4	0.0	20.4	0.719648	166958	167499	1001-1001	0.142857	40.0
orf193_p1	orf193	1	CATGGGTATTGTTCCTCGGA	GTCACTTATGGCATGCAGGA	20	20	50.0	50.0	57.0	58.0	0.0	21.4	1.043071	168405	168986	1216-1216	0.428571	56.0
orf193_p2	orf193	2	TCATGGGTATTGTTCCTCGG	GTCACTTATGGCATGCAGGA	20	20	50.0	50.0	57.0	58.0	0.0	21.4	1.043071	168405	168986	1217-1217	0.428571	56.0
orf193_p3	orf193	3	CATGGGTATTGTTCCTCGGA	TCAACCTTGGCAAGAACGAA	20	20	50.0	45.0	57.0	58.0	0.0	28.2	1.045946	168405	168986	1383-1383	0.428571	55.8
orf193_p4	orf193	4	CATGGGTATTGTTCCTCGGA	TTCAACCTTGGCAAGAACGA	20	20	50.0	45.0	57.0	58.0	0.0	28.2	1.045946	168405	168986	1384-1384	0.428571	55.8
orf193_p5	orf193	5	TCATGGGTATTGTTCCTCGG	TCAACCTTGGCAAGAACGAA	20	20	50.0	45.0	57.0	58.0	0.0	28.2	1.045946	168405	168986	1384-1384	0.428571	55.8
psbD-ompR_p1	psbD-ompR	1	GTCGCTCCATGAATAGCACA	TGAAGATGACCCAAGCAACC	20	20	50.0	50.0	58.1	58.1	13.0	0.0	0.150326	171705	172232	1263-1263	0.142857	44.5
psbD-ompR_p2	psbD-ompR	2	CAACTCCAAAGCTAGGTGCA	TGAAGATGACCCAAGCAACC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.194081	171705	172232	1139-1139	0.142857	44.2
psbD-ompR_p3	psbD-ompR	3	TGAACTGGCCAATCCATGAG	TGAAGATGACCCAAGCAACC	20	20	50.0	50.0	57.9	58.1	7.0	0.0	0.223582	171705	172232	811-811	0.142857	43.9
psbD-ompR_p4	psbD-ompR	4	GAACTGGCCAATCCATGAGT	TGAAGATGACCCAAGCAACC	20	20	50.0	50.0	57.9	58.1	7.0	0.0	0.225089	171705	172232	810-810	0.142857	43.9
psbD-ompR_p5	psbD-ompR	5	GTCGCTCCATGAATAGCACA	TAGTAAGTCGTGCGGGAGAA	20	20	50.0	50.0	58.1	58.2	13.0	0.0	0.234935	171705	172232	1390-1390	0.142857	43.8