primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	CAGGATCAGTCGTGGTCTTG	GACAAGTCGGCCTAGATGTG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.073917	4204	6415	2457-2457	0.333333	52.7
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	CAGGATCAGTCGTGGTCTTG	TCTTGACAAGTCGGCCTAGA	20	20	55.0	50.0	58.0	58.1	0.0	14.7	0.082572	4204	6415	2461-2461	0.333333	52.7
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	CCTTTCAGGATCAGTCGTGG	GACAAGTCGGCCTAGATGTG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.087841	4204	6415	2462-2462	0.333333	52.6
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	CCTTTCAGGATCAGTCGTGG	TCTTGACAAGTCGGCCTAGA	20	20	55.0	50.0	58.0	58.1	0.0	14.7	0.096496	4204	6415	2466-2466	0.333333	52.6
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	CAGGATCAGTCGTGGTCTTG	TCAACGCCTCTTGACAAGTC	20	20	55.0	50.0	58.0	58.1	0.0	14.7	0.135947	4204	6415	2469-2469	0.333333	52.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	AGTCCACTCAGCCATCTCTC	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.5	55.5	0.0	0.0	4.051804	8046	8811	821-952	1.000000	59.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AGTCCACTCAGCCATCTCTC	GAACGAATCACACTTTTACCACT	20	23	55.0	39.1	58.5	57.3	0.0	0.0	4.184520	8046	8811	823-954	1.000000	58.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AGTCCACTCAGCCATCTCTC	AGAACGAATCACACTTTTACCAC	20	23	55.0	39.1	58.5	57.3	0.0	0.0	4.184520	8046	8811	824-955	1.000000	58.4
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGTCCACTCAGCCATCTCTC	AACGAATCACACTTTTACCACT	20	22	55.0	36.4	58.5	56.2	0.0	0.0	4.292275	8046	8811	822-953	1.000000	57.6
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	TAGTCCACTCAGCCATCTCT	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.2	55.5	0.0	0.0	4.372479	8046	8811	822-953	1.000000	57.0
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	13534	14266	820-1034	1.000000	87.0
atpH-atpI_p2	atpH-atpI	2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	13534	14266	821-1035	1.000000	85.8
atpH-atpI_p3	atpH-atpI	3	TCCAGCAGCAATAACGGAAG	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.394902	13534	14266	830-1044	1.000000	85.1
atpH-atpI_p4	atpH-atpI	4	ATAACGGAAGCGGCAGAAAT	ATCCATGGAGGGTCATCACT	20	20	45.0	50.0	58.0	57.8	1.4	21.3	0.241507	13534	14266	787-787	0.333333	51.4
atpH-atpI_p5	atpH-atpI	5	AATAACGGAAGCGGCAGAAA	ATCCATGGAGGGTCATCACT	20	20	45.0	50.0	58.2	57.8	1.4	21.3	0.387046	13534	14266	788-788	0.333333	50.2
rps2-rpoC2_p1	rps2-rpoC2	1	CATCATTGGCAGGAATCGGA	TTATCGATCCCAGGGGGTAC	20	20	50.0	55.0	58.0	58.0	0.0	5.3	0.048326	15934	16563	1742-1742	0.333333	52.9
rps2-rpoC2_p2	rps2-rpoC2	2	TCATCATTGGCAGGAATCGG	TTATCGATCCCAGGGGGTAC	20	20	50.0	55.0	58.0	58.0	0.0	5.3	0.048326	15934	16563	1743-1743	0.333333	52.9
rps2-rpoC2_p3	rps2-rpoC2	3	CTCCTGCTTTGATCATGCCT	TTATCGATCCCAGGGGGTAC	20	20	50.0	55.0	57.9	58.0	15.6	5.3	0.074039	15934	16563	1169-1169	0.333333	52.7
rps2-rpoC2_p4	rps2-rpoC2	4	TGACCAAAATGGACTCCTGC	TTATCGATCCCAGGGGGTAC	20	20	50.0	55.0	58.1	58.0	2.9	5.3	0.110108	15934	16563	1182-1182	0.333333	52.5
rps2-rpoC2_p5	rps2-rpoC2	5	ACCGTGACCAAAATGGACTC	TTATCGATCCCAGGGGGTAC	20	20	50.0	55.0	58.1	58.0	2.9	5.3	0.127598	15934	16563	1186-1186	0.333333	52.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	AGTTCAAATCTGGGTGTCGC	CTCCTCCCCCATACTACGAG	20	20	50.0	60.0	58.5	58.1	0.0	0.0	0.563826	27806	28493	790-826	1.000000	85.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTTGTTTTGGCGACATGGC	CTCCTCCCCCATACTACGAG	20	20	45.0	60.0	58.4	58.1	0.0	0.0	0.511227	27806	28493	848-884	1.000000	85.0
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTTGTTTTGGCGACATGGC	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	58.4	58.2	0.0	0.0	0.574992	27806	28493	795-831	1.000000	85.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTA	CTCCTCCCCCATACTACGAG	20	20	50.0	60.0	57.5	58.1	14.7	0.0	0.612688	27806	28493	815-851	1.000000	84.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	GACATGGCCAAGTGGTAAGG	CTCCTCCCCCATACTACGAG	20	20	55.0	60.0	58.5	58.1	20.2	0.0	0.623909	27806	28493	836-872	1.000000	84.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGAACTAGAATAAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	24	20	37.5	50.0	57.4	58.5	0.0	5.4	5.090485	30018	30836	937-1066	1.000000	47.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGTAAAAAGGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	38.1	50.0	54.5	58.5	0.0	5.4	5.014388	30018	30836	951-1080	1.000000	47.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAGTAAAAAGGCAGTAGGAACT	TTCAATTGGTCAGAGCACCG	22	20	36.4	50.0	55.3	58.5	0.0	5.4	5.194981	30018	30836	952-1081	1.000000	47.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAAAGGCAGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	20	20	40.0	50.0	53.3	58.5	0.0	5.4	5.213648	30018	30836	947-1076	1.000000	47.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AAAGGCAGTAGGAACTAGAA	TTCAATTGGTCAGAGCACCG	20	20	40.0	50.0	53.3	58.5	0.0	5.4	5.213648	30018	30836	946-1075	1.000000	47.2
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.828828	30911	31254	425-443	1.000000	83.3
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	20	20	50.0	50.0	58.5	57.6	7.1	0.0	0.852505	30911	31254	424-442	1.000000	83.1
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	CGGTGCTCTGACCAATTGAA	GATTCTTCCTGGGTCGATGC	20	20	50.0	55.0	58.5	58.4	7.1	0.0	0.889785	30911	31254	458-476	1.000000	82.4
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	CGGTGCTCTGACCAATTGAA	TGGGGACGGACTGTAAATTC	20	20	50.0	50.0	58.5	57.2	7.1	0.0	1.251324	30911	31254	426-444	1.000000	79.9
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GGTGCTCTGACCAATTGAAC	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	57.0	58.4	7.1	0.0	1.348981	30911	31254	424-442	1.000000	79.1
psaA-ycf3_p1	psaA-ycf3	1	TTTCACTTCTTGTTCCGGCG	TTGGTTGAAGATCACAGGGC	20	20	50.0	50.0	59.1	58.1	0.0	0.0	1.148758	42145	42731	651-675	1.000000	80.5
psaA-ycf3_p2	psaA-ycf3	2	TTTTCACTTCTTGTTCCGGC	TTGGTTGAAGATCACAGGGC	20	20	45.0	50.0	56.9	58.1	0.0	0.0	1.238138	42145	42731	652-676	1.000000	79.7
psaA-ycf3_p3	psaA-ycf3	3	CACTTCTTGTTCCGGCGAA	TTGGTTGAAGATCACAGGGC	19	20	52.6	50.0	58.4	58.1	9.3	0.0	1.478100	42145	42731	648-672	1.000000	77.9
psaA-ycf3_p4	psaA-ycf3	4	TTCACTTCTTGTTCCGGCG	TTGGTTGAAGATCACAGGGC	19	20	52.6	50.0	58.4	58.1	0.0	0.0	1.478100	42145	42731	650-674	1.000000	77.8
psaA-ycf3_p5	psaA-ycf3	5	ATTTTCACTTCTTGTTCCGGC	TTGGTTGAAGATCACAGGGC	21	20	42.9	50.0	57.4	58.1	0.0	0.0	1.733459	42145	42731	653-677	1.000000	75.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	46602	47469	918-930	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	46602	47469	926-938	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	46602	47469	932-944	1.000000	89.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	CTAACCTCTGAGCTAAGCGG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.5	58.0	5.5	0.0	0.505133	46602	47469	910-922	1.000000	88.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	46602	47469	920-932	1.000000	87.8
accD_p1	accD	1	TGTCTTTCTCGTTGCCTGTT	ATGAGATTTGGTCCCCCTCA	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.339961	56912	58609	2551-2551	0.333333	50.6
accD_p2	accD	2	TGTCTTTCTCGTTGCCTGTT	TGAGATTTGGTCCCCCTCAT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.339961	56912	58609	2550-2550	0.333333	50.6
accD_p3	accD	3	TTGTCTTTCTCGTTGCCTGT	ATGAGATTTGGTCCCCCTCA	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.339961	56912	58609	2552-2552	0.333333	50.6
accD_p4	accD	4	TTGTCTTTCTCGTTGCCTGT	TGAGATTTGGTCCCCCTCAT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.339961	56912	58609	2551-2551	0.333333	50.6
accD_p5	accD	5	TGTCTTTCTCGTTGCCTGTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.7	58.0	0.0	26.9	0.352586	56912	58609	2487-2487	0.333333	50.5
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GGAACAGATTCGGTGGAGAC	GGCGAATGGCATTCTTCCTA	20	20	55.0	50.0	58.0	58.0	0.0	31.1	0.044222	107251	107579	1699-1699	0.333333	53.0
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GGAACAGATTCGGTGGAGAC	CCTTCTTCCATAACCCGAGC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.056613	107251	107579	1164-1164	0.333333	52.9
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GGAACAGATTCGGTGGAGAC	ATTTTAGGCGGATGTGGGAC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.064322	107251	107579	1598-1598	0.333333	52.8
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GGAACAGATTCGGTGGAGAC	TATGAACATCACGAGCTGCC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.071566	107251	107579	1225-1225	0.333333	52.8
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	ATTCGAACCTACGGCCAATC	GGCGAATGGCATTCTTCCTA	20	20	50.0	50.0	58.1	58.0	2.2	31.1	0.083216	107251	107579	1072-1072	0.333333	52.7
ycf1_p1	ycf1	1	AGGGCAAAGACAAAGACAGG	ACGTAGCATATACAGCCCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024240	110186	110435	968-968	0.333333	53.1
ycf1_p2	ycf1	2	AAACGCAAACGCAAAGACAA	ACGTAGCATATACAGCCCCT	20	20	40.0	50.0	58.0	58.0	0.0	0.0	0.027150	110186	110435	939-939	0.333333	53.1
ycf1_p3	ycf1	3	AAAGACAAACGCAAACGCAA	ACGTAGCATATACAGCCCCT	20	20	40.0	50.0	58.0	58.0	0.0	0.0	0.027150	110186	110435	945-945	0.333333	53.1
ycf1_p4	ycf1	4	AACGCAAACGCAAAGACAAA	ACGTAGCATATACAGCCCCT	20	20	40.0	50.0	58.0	58.0	0.0	0.0	0.027150	110186	110435	938-938	0.333333	53.1
ycf1_p5	ycf1	5	AAGACAAACGCAAACGCAAA	ACGTAGCATATACAGCCCCT	20	20	40.0	50.0	58.0	58.0	0.0	0.0	0.027150	110186	110435	944-944	0.333333	53.1
ndhH_p1	ndhH	1	TTGATGAACCTCGTTTTGAG	AGCTTGTACTTCCATTATCAT	20	21	40.0	33.3	53.9	52.3	0.0	0.0	10.842421	114734	115915	1257-1257	0.333333	13.3
ndhH_p2	ndhH	2	TTGATGAACCTCGTTTTGAG	AGCTTGTACTTCCATTATCATT	20	22	40.0	31.8	53.9	53.2	0.0	0.0	10.923842	114734	115915	1257-1257	0.333333	13.3
ndhH_p3	ndhH	3	TTTGATGAACCTCGTTTTGAG	AGCTTGTACTTCCATTATCAT	21	21	38.1	33.3	54.7	52.3	0.0	0.0	10.984412	114734	115915	1258-1258	0.333333	13.3
ndhH_p4	ndhH	4	TTTGATGAACCTCGTTTTGAG	AGCTTGTACTTCCATTATCATT	21	22	38.1	31.8	54.7	53.2	0.0	0.0	11.065833	114734	115915	1258-1258	0.333333	13.3
ndhH_p5	ndhH	5	TTTTGATGAACCTCGTTTTGAG	AGCTTGTACTTCCATTATCAT	22	21	36.4	33.3	55.5	52.3	0.0	0.0	11.202528	114734	115915	1259-1259	0.333333	13.3
ndhF-rpl32_p1	ndhF-rpl32	1	ACAGGAAGTGGAAGGAAAGG	AGCCTTAACTGCAGCCAAAT	20	20	50.0	45.0	57.0	58.1	0.0	15.3	1.030597	118372	119359	1134-1244	1.000000	83.7
ndhF-rpl32_p2	ndhF-rpl32	2	CAGGAAGTGGAAGGAAAGGT	AGCCTTAACTGCAGCCAAAT	20	20	50.0	45.0	57.0	58.1	0.0	15.3	1.030597	118372	119359	1133-1243	1.000000	83.7
ndhF-rpl32_p3	ndhF-rpl32	3	CAGGAAGTGGAAGGAAAGGT	AAAAGCCTTAACTGCAGCCA	20	20	50.0	45.0	57.0	58.3	0.0	15.3	1.256054	118372	119359	1136-1246	1.000000	81.9
ndhF-rpl32_p4	ndhF-rpl32	4	CAGGAAGTGGAAGGAAAGGT	AAAGCCTTAACTGCAGCCAA	20	20	50.0	45.0	57.0	58.3	0.0	15.3	1.256054	118372	119359	1135-1245	1.000000	81.9
ndhF-rpl32_p5	ndhF-rpl32	5	CAGGAAGTGGAAGGAAAGGT	AAGCCTTAACTGCAGCCAAA	20	20	50.0	45.0	57.0	58.3	0.0	15.3	1.256054	118372	119359	1134-1244	1.000000	81.9