primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnH-GUG-psbA_p1 trnH-GUG-psbA 1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 20 20 55.0 55.0 58.1 58.3 0.0 0.0 0.465225 77 423 438-470 1.000000 88.3 trnH-GUG-psbA_p2 trnH-GUG-psbA 2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.469587 77 423 452-484 1.000000 88.2 trnH-GUG-psbA_p3 trnH-GUG-psbA 3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.471094 77 423 451-483 1.000000 88.2 trnH-GUG-psbA_p4 trnH-GUG-psbA 4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 20 20 50.0 55.0 57.9 58.3 0.0 0.0 0.471094 77 423 453-485 1.000000 88.2 trnH-GUG-psbA_p5 trnH-GUG-psbA 5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 20 20 55.0 50.0 58.1 57.6 0.0 0.0 0.525510 77 423 439-471 1.000000 87.8 trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 20 20 55.0 50.0 58.0 57.9 0.0 0.0 0.103840 4277 5141 1186-1246 0.964286 89.7 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 CCCTTTCAGGATCAGTCGTG TGTTGCTGCCGTTTTGAAAG 20 20 55.0 45.0 58.0 58.1 0.0 0.0 0.095818 4277 5141 1634-1693 0.964286 89.3 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 CCTTTCAGGATCAGTCGTGG TGTTGCTGCCGTTTTGAAAG 20 20 55.0 45.0 58.0 58.1 0.0 0.0 0.095818 4277 5141 1633-1692 0.964286 89.3 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 20 20 55.0 50.0 58.0 57.9 0.0 18.8 0.091715 4277 5141 1267-1305 0.928571 87.7 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 20 20 55.0 50.0 58.0 57.9 0.0 18.8 0.091715 4277 5141 1266-1304 0.928571 87.7 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 57.9 58.1 7.8 16.9 0.156387 6257 7910 1236-1753 1.000000 90.7 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 20 20 45.0 55.0 58.1 58.1 8.1 16.9 0.215425 6257 7910 1219-1736 1.000000 90.3 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 20 20 50.0 55.0 58.2 58.1 7.8 16.9 0.285346 6257 7910 1237-1754 1.000000 89.7 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 20 20 55.0 55.0 58.4 58.1 7.8 16.9 0.445953 6257 7910 1234-1751 1.000000 88.4 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 20 20 55.0 55.0 58.4 58.1 2.9 16.9 0.445953 6257 7910 1233-1750 1.000000 88.4 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 CAATCCGACGCTTTAGTCCA AGGGGGTTAATAGAACGAATCAC 20 23 50.0 43.5 58.0 58.0 0.0 0.0 3.036909 9117 9849 810-852 1.000000 67.7 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 AATCCGACGCTTTAGTCCAC AGGGGGTTAATAGAACGAATCAC 20 23 50.0 43.5 58.0 58.0 0.0 0.0 3.038297 9117 9849 809-851 1.000000 67.7 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 ACGGAAAGAGAGGGATTCGA AGGGGGTTAATAGAACGAATCAC 20 23 50.0 43.5 58.2 58.0 0.0 0.0 3.183675 9117 9849 864-906 1.000000 66.5 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 CAATCCGACGCTTTAGTCCA GGGGGTTAATAGAACGAATCACA 20 23 50.0 43.5 58.0 58.2 0.0 0.0 3.231570 9117 9849 809-851 1.000000 66.1 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 AATCCGACGCTTTAGTCCAC GGGGGTTAATAGAACGAATCACA 20 23 50.0 43.5 58.0 58.2 0.0 0.0 3.232958 9117 9849 808-850 1.000000 66.1 atpH-atpI_p1 atpH-atpI 1 ATAACGGAAGCGGCAGAAAT CAAGCGGTATCCAAGCTCTT 20 20 45.0 50.0 58.0 58.0 1.4 0.0 0.068225 14408 15541 1011-1303 1.000000 91.5 atpH-atpI_p2 atpH-atpI 2 TACCTTGACCAACTCCAGGT CAAGCGGTATCCAAGCTCTT 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.142117 14408 15541 1067-1359 1.000000 90.9 atpH-atpI_p3 atpH-atpI 3 AATAACGGAAGCGGCAGAAA CAAGCGGTATCCAAGCTCTT 20 20 45.0 50.0 58.2 58.0 1.4 0.0 0.213764 14408 15541 1012-1304 1.000000 90.3 atpH-atpI_p4 atpH-atpI 4 GCAGTACCTTGACCAACTCC CAAGCGGTATCCAAGCTCTT 20 20 55.0 50.0 58.2 58.0 0.0 0.0 0.220154 14408 15541 1071-1363 1.000000 90.2 atpH-atpI_p5 atpH-atpI 5 TACCTTCTACAGCTTGGCCT CAAGCGGTATCCAAGCTCTT 20 20 50.0 50.0 58.0 58.0 3.3 0.0 0.077217 14408 15541 1094-1376 0.928571 88.4 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 20 20 55.0 45.0 58.1 58.2 7.6 0.0 0.310137 32933 34339 1519-1573 1.000000 89.4 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 20 20 55.0 50.0 58.1 57.7 7.6 9.5 0.323334 32933 34339 1533-1587 1.000000 89.3 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.332292 32933 34339 1522-1576 1.000000 89.2 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 TCAGTGGTAGAGTAACGCCA GACCGGACCAACCTACAAAA 20 20 50.0 50.0 58.1 57.7 0.0 9.5 0.345488 32933 34339 1536-1590 1.000000 89.1 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 GTGGTAGAGTAACGCCATGG TAGACCGGACCAACCTACAA 20 20 55.0 50.0 58.1 57.7 7.6 9.5 0.371563 32933 34339 1535-1589 1.000000 88.9 trnG-GCC-trnM-CAU_p1 trnG-GCC-trnM-CAU 1 TTGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.147952 38004 38198 326-349 1.000000 90.8 trnG-GCC-trnM-CAU_p2 trnG-GCC-trnM-CAU 2 TTGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 20 20 50.0 50.0 58.1 58.0 0.0 4.2 0.160570 38004 38198 290-313 1.000000 90.7 trnG-GCC-trnM-CAU_p3 trnG-GCC-trnM-CAU 3 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 20 20 50.0 55.0 57.8 58.0 0.0 0.0 0.195421 38004 38198 297-320 1.000000 90.4 trnG-GCC-trnM-CAU_p4 trnG-GCC-trnM-CAU 4 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 20 20 50.0 50.0 57.8 58.0 0.0 4.2 0.208039 38004 38198 261-284 1.000000 90.3 trnG-GCC-trnM-CAU_p5 trnG-GCC-trnM-CAU 5 TTGCGGATATGGTCGAATGG GTCACGGGTTCAAATCCTGT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.230154 38004 38198 284-307 1.000000 90.2 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 57.9 58.0 0.0 0.0 0.137364 48185 49097 285-963 1.000000 90.9 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 GAGTCGAACCGATGACCATC ACCAATTTCGCCATATCCCC 20 20 55.0 50.0 58.2 58.0 0.0 0.0 0.163174 48185 49097 316-994 1.000000 90.7 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 57.9 58.1 0.0 0.0 0.202173 48185 49097 285-971 1.000000 90.4 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 GAGTCGAACCGATGACCATC TAGCGTCTACCAATTTCGCC 20 20 55.0 50.0 58.2 58.1 0.0 0.0 0.227983 48185 49097 316-1002 1.000000 90.2 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 20 20 55.0 50.0 57.9 58.2 0.0 10.2 0.372548 48185 49097 284-977 1.000000 89.0 trnF-GAA-ndhJ_p1 trnF-GAA-ndhJ 1 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 20 20 55.0 50.0 58.0 58.1 10.3 0.0 0.089643 50139 50822 737-2353 1.000000 91.2 trnF-GAA-ndhJ_p2 trnF-GAA-ndhJ 2 CCTCGTGTCACCAGTTCAAA AGTTGGATAGGATGGCCCTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.139034 50139 50822 701-2353 1.000000 90.9 trnF-GAA-ndhJ_p3 trnF-GAA-ndhJ 3 CTGGTAGAGCAGAGGACTGA AGTTGGATAGGATGGCCCTT 20 20 55.0 50.0 57.9 58.1 2.5 0.0 0.223436 50139 50822 725-2353 1.000000 90.2 trnF-GAA-ndhJ_p4 trnF-GAA-ndhJ 4 GGGATAGCTCAGCTGGTAGA ATCATCCACGCTTGAAACGT 20 20 55.0 45.0 58.0 58.2 10.3 0.0 0.194095 50139 50822 772-2388 1.000000 90.0 trnF-GAA-ndhJ_p5 trnF-GAA-ndhJ 5 CCTCGTGTCACCAGTTCAAA ATCATCCACGCTTGAAACGT 20 20 50.0 45.0 58.1 58.2 0.0 0.0 0.243485 50139 50822 736-2388 1.000000 89.9 ndhC-trnV-UAC_p1 ndhC-trnV-UAC 1 TCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 22 20 31.8 55.0 53.9 58.1 0.0 0.0 6.230058 52494 53649 945-1239 1.000000 52.0 ndhC-trnV-UAC_p2 ndhC-trnV-UAC 2 ATCATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 23 20 30.4 55.0 54.5 58.1 0.0 0.0 6.643914 52494 53649 946-1240 1.000000 52.0 ndhC-trnV-UAC_p3 ndhC-trnV-UAC 3 TCATATTCGTAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 23 20 30.4 55.0 54.5 58.1 0.0 0.0 6.643914 52494 53649 945-1239 1.000000 52.0 ndhC-trnV-UAC_p4 ndhC-trnV-UAC 4 TCATATTCGTAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 22 20 31.8 55.0 53.9 58.7 0.0 0.0 6.805875 52494 53649 930-1224 1.000000 52.0 ndhC-trnV-UAC_p5 ndhC-trnV-UAC 5 CATATTCGTAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 21 20 33.3 55.0 52.3 58.1 0.0 0.0 6.808247 52494 53649 944-1238 1.000000 52.0 accD-psaI_p1 accD-psaI 1 AACGCCTACATTGCATTTGC CCATTGCAATTGCCGGAAAT 20 20 45.0 45.0 58.0 58.0 0.0 26.9 0.032087 60924 61581 841-932 1.000000 91.7 accD-psaI_p2 accD-psaI 2 AACGCCTACATTGCATTTGC TACATTTCAGACATCCGCCG 20 20 45.0 50.0 58.0 58.1 0.0 0.0 0.070495 60924 61581 1090-1181 1.000000 91.4 accD-psaI_p3 accD-psaI 3 AACGCCTACATTGCATTTGC TCCGCCGATCCTGATCTATT 20 20 45.0 50.0 58.0 58.1 0.0 5.7 0.080353 60924 61581 1077-1168 1.000000 91.4 accD-psaI_p4 accD-psaI 4 ACCCGTTTGATCGAGTATGC CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.1 58.0 0.0 26.9 0.091071 60924 61581 1081-1172 1.000000 91.3 accD-psaI_p5 accD-psaI 5 TCGATTGATCCAGGCACTTG CCATTGCAATTGCCGGAAAT 20 20 50.0 45.0 58.0 58.0 0.0 26.9 0.055423 60924 61581 1386-1412 0.678571 77.1 ycf4-cemA_p1 ycf4-cemA 1 TTGGCCTATTTCTTGCGTGT AGAAGAGGAGTGAATGCTTTCT 20 22 45.0 40.9 58.1 57.1 3.3 13.0 3.002460 62672 63548 715-976 1.000000 68.0 ycf4-cemA_p2 ycf4-cemA 2 AAAAAGCTGCTGAATTGGCC AGAAGAGGAGTGAATGCTTTCT 20 22 45.0 40.9 57.8 57.1 1.4 13.0 3.081110 62672 63548 729-990 1.000000 67.4 ycf4-cemA_p3 ycf4-cemA 3 TTGGCCTATTTCTTGCGTGT TGCGAGATATAGAAGAGGAGTGA 20 23 45.0 43.5 58.1 58.0 3.3 0.0 3.121315 62672 63548 725-986 1.000000 67.0 ycf4-cemA_p4 ycf4-cemA 4 AAAGCTGCTGAATTGGCCTA AGAAGAGGAGTGAATGCTTTCT 20 22 45.0 40.9 57.8 57.1 1.4 13.0 3.127400 62672 63548 727-988 1.000000 67.0 ycf4-cemA_p5 ycf4-cemA 5 AAAAAGCTGCTGAATTGGCC TGCGAGATATAGAAGAGGAGTGA 20 23 45.0 43.5 57.8 58.0 1.4 0.0 3.199965 62672 63548 739-1000 1.000000 66.4 petA-psbJ_p1 petA-psbJ 1 TCAGAGCTATCGCCCCAATA GGAAGTAGGGTAAATGGCCG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.046509 65453 66440 1676-1708 1.000000 91.1 petA-psbJ_p2 petA-psbJ 2 TCAGAGCTATCGCCCCAATA AACTGGCCGTTTTGATCCTT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.014305 65453 66440 2111-2159 1.000000 90.3 petA-psbJ_p3 petA-psbJ 3 TCAGAGCTATCGCCCCAATA ACTGGCCGTTTTGATCCTTT 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.014305 65453 66440 2110-2158 1.000000 90.3 petA-psbJ_p4 petA-psbJ 4 TCAGAGCTATCGCCCCAATA ACACAATCAAACCCGAACGA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.053385 65453 66440 1907-1939 0.964286 88.7 petA-psbJ_p5 petA-psbJ 5 TCAGAGCTATCGCCCCAATA ACGACACAATCAAACCCGAA 20 20 50.0 45.0 58.0 58.0 0.0 0.0 0.053385 65453 66440 1910-1942 0.785714 79.6 ndhF-rpl32_p1 ndhF-rpl32 1 GTTGCCGTCGGAAAAAGAAG AAACGAGAGGTACATGGGGA 20 20 50.0 50.0 58.0 58.1 17.5 0.0 0.085430 115126 116268 1519-1914 0.607143 75.6 ndhF-rpl32_p2 ndhF-rpl32 2 GTTGCCGTCGGAAAAAGAAG GATATCAGAAGAGCGAGGCG 20 20 50.0 55.0 58.0 58.0 17.5 0.0 0.056577 115126 116268 1528-1562 0.392857 64.5 ndhF-rpl32_p3 ndhF-rpl32 3 TGCCGTCGGAAAAAGAAGAA GATATCAGAAGAGCGAGGCG 20 20 45.0 55.0 58.0 58.0 17.5 0.0 0.067867 115126 116268 1526-1560 0.392857 64.5 ndhF-rpl32_p4 ndhF-rpl32 4 TTGCCGTCGGAAAAAGAAGA GATATCAGAAGAGCGAGGCG 20 20 45.0 55.0 58.0 58.0 17.5 0.0 0.067867 115126 116268 1527-1561 0.392857 64.5 ndhF-rpl32_p5 ndhF-rpl32 5 CAAGAGGTCGTGTGAACCAA GATATCAGAAGAGCGAGGCG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.076059 115126 116268 1941-1975 0.392857 63.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TTTCCTTGAGCTTGGCCAAT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.0 58.0 20.8 0.0 0.021840 116431 117360 1583-1644 0.714286 79.4 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TTTCCTTGAGCTTGGCCAAT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 58.0 58.0 20.8 0.0 0.023211 116431 117360 1584-1645 0.714286 79.4 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TTCCTTGAGCTTGGCCAATT AGCGTGTCTACCGATTTCAC 20 20 45.0 50.0 58.0 58.0 20.8 0.0 0.021840 116431 117360 1582-1643 0.678571 78.0 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TTCCTTGAGCTTGGCCAATT CAGCGTGTCTACCGATTTCA 20 20 45.0 50.0 58.0 58.0 20.8 0.0 0.023211 116431 117360 1583-1644 0.678571 77.9 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TTCCTTGAGCTTGGCCAATT GCACTTGGAACGGTTATTGC 20 20 45.0 50.0 58.0 58.0 20.8 0.0 0.036821 116431 117360 2046-2106 0.678571 76.3 ccsA-ndhD_p1 ccsA-ndhD 1 GCAATAACCGTTCCAAGTGC TGCATTACCGGGAATGAGTG 20 20 50.0 50.0 58.0 58.0 0.0 38.4 0.059769 118503 118783 1263-1297 1.000000 89.9 ccsA-ndhD_p2 ccsA-ndhD 2 GCAATAACCGTTCCAAGTGC TTATCTCGACGAAATGGGCG 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.174994 118503 118783 1344-1378 1.000000 88.6 ccsA-ndhD_p3 ccsA-ndhD 3 GCAATAACCGTTCCAAGTGC GGGACTCAATGGAGCCATTT 20 20 50.0 50.0 58.0 57.9 0.0 11.9 0.172785 118503 118783 1443-1477 1.000000 88.2 ccsA-ndhD_p4 ccsA-ndhD 4 GCAATAACCGTTCCAAGTGC TTCAACATCTCCTGGCCAAC 20 20 50.0 50.0 58.0 58.1 0.0 7.0 0.113112 118503 118783 1545-1579 1.000000 88.1 ccsA-ndhD_p5 ccsA-ndhD 5 GCAATAACCGTTCCAAGTGC GCATATGGGTTGGTTCGGAT 20 20 50.0 50.0 58.0 58.0 0.0 4.6 0.041555 118503 118783 1649-1683 0.964286 86.7 ycf1_p1 ycf1 1 TCCACTTCTTTTATTTGTTCTTCG GCAACTGTTTCAAGCAAATG 24 20 33.3 40.0 55.9 54.6 0.0 0.0 9.479089 130403 130522 174-180 1.000000 48.0 ycf1_p2 ycf1 2 TCCACTTCTTTTATTTGTTCTTCGG GCAACTGTTTCAAGCAAATG 25 20 36.0 40.0 58.0 54.6 0.0 0.0 8.418422 130403 130522 174-180 0.928571 45.1 ycf1_p3 ycf1 3 CCACTTCTTTTATTTGTTCTTCGG GCAACTGTTTCAAGCAAATG 24 20 37.5 40.0 56.8 54.6 0.0 0.0 8.615501 130403 130522 173-179 0.928571 45.1 ycf1_p4 ycf1 4 CCACTTCTTTTATTTGTTCTTCGGT GCAACTGTTTCAAGCAAATG 25 20 36.0 40.0 58.3 54.6 0.0 0.0 8.649313 130403 130522 173-179 0.928571 45.1 ycf1_p5 ycf1 5 CACTTCTTTTATTTGTTCTTCGGT GCAACTGTTTCAAGCAAATG 24 20 33.3 40.0 56.1 54.6 0.0 0.0 9.238938 130403 130522 172-178 0.928571 45.1