primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5538	6322	843-859	1.000000	89.8
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5538	6322	854-870	1.000000	89.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5538	6322	847-863	1.000000	88.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	20	20	45.0	45.0	58.1	57.8	8.1	0.0	0.312175	5538	6322	912-928	1.000000	88.5
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.377084	5538	6322	923-939	1.000000	87.9
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	GTCGACCCAAGCGGATATAC	TCTGGTACTACAGAGCGGAG	20	20	55.0	55.0	57.9	58.0	3.5	0.0	0.101558	12197	12642	644-646	1.000000	80.8
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GCGAGCGTAGTTCAATGGTA	TCTGGTACTACAGAGCGGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.122546	12197	12642	604-606	1.000000	80.7
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	TATACCGTAGCGAGCGTAGT	TCTGGTACTACAGAGCGGAG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.194672	12197	12642	613-615	1.000000	80.1
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	CGTAGCGAGCGTAGTTCAAT	TCTGGTACTACAGAGCGGAG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.215990	12197	12642	608-610	1.000000	80.0
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	TAGCGAGCGTAGTTCAATGG	TCTGGTACTACAGAGCGGAG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.243618	12197	12642	606-608	1.000000	79.8
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	21	20	42.9	55.0	57.6	58.1	0.0	0.0	1.526483	16827	17562	820-859	1.000000	70.3
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	21	20	42.9	55.0	57.6	58.1	0.0	0.0	1.526483	16827	17562	819-858	1.000000	70.3
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	21	20	42.9	50.0	57.6	58.2	0.0	0.0	1.558772	16827	17562	878-917	1.000000	69.9
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	21	20	42.9	55.0	57.6	57.8	0.0	0.0	1.583671	16827	17562	818-857	1.000000	69.8
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	21	20	42.9	60.0	57.6	58.2	0.0	6.7	1.624352	16827	17562	815-854	1.000000	69.5
atpI_p1	atpI	1	AAATTGTGACCCGGATCTCG	TGGTGAGTAGTTCCTCGTGT	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.033055	31125	31868	1709-1710	1.000000	81.3
atpI_p2	atpI	2	AAATTGTGACCCGGATCTCG	TTCTGGCTGTCTCGCAATAC	20	20	50.0	50.0	58.0	58.0	0.7	0.0	0.034321	31125	31868	1840-1841	1.000000	81.2
atpI_p3	atpI	3	ATCAGCTGCAATAAGGGCTC	TGGTGAGTAGTTCCTCGTGT	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.030425	31125	31868	2033-2034	1.000000	81.1
atpI_p4	atpI	4	ATCAGCTGCAATAAGGGCTC	TTCTGGCTGTCTCGCAATAC	20	20	50.0	50.0	58.0	58.0	9.5	0.0	0.031691	31125	31868	2164-2165	1.000000	81.0
atpI_p5	atpI	5	ATCAGCTGCAATAAGGGCTC	CCCGCATAAAAGAGGACTCC	20	20	50.0	55.0	58.0	58.0	9.5	0.0	0.066619	31125	31868	1719-1719	1.000000	80.6
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	TTCGAATCCCTCTCTTTCCG	ATAGGAAGTGGGTCGGTCTC	20	20	50.0	55.0	57.0	58.2	0.0	0.0	1.183586	45039	45350	389-400	1.000000	75.0
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	ATGTAGGAAAGATGGCCGAG	AGATAGGAAGTGGGTCGGTC	20	20	50.0	55.0	57.1	58.2	0.0	0.0	1.140771	45039	45350	463-474	1.000000	74.7
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	ATGTAGGAAAGATGGCCGAG	ATAGGAAGTGGGTCGGTCTC	20	20	50.0	55.0	57.1	58.2	0.0	0.0	1.140771	45039	45350	461-472	1.000000	74.7
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	ATGTAGGAAAGATGGCCGAG	GATAGGAAGTGGGTCGGTCT	20	20	50.0	55.0	57.1	58.2	0.0	0.0	1.140771	45039	45350	462-473	1.000000	74.7
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	ATGTAGGAAAGATGGCCGAG	GAAGTGGGTCGGTCTCAAAA	20	20	50.0	50.0	57.1	57.8	0.0	0.0	1.168555	45039	45350	457-468	1.000000	74.5
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	46329	46936	709-724	1.000000	81.1
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	46329	46936	701-716	1.000000	80.8
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	46329	46936	679-694	1.000000	80.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	46329	46936	699-714	1.000000	79.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	46329	46936	708-723	1.000000	78.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175907	47975	48561	721-722	1.000000	79.9
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	47975	48561	696-697	1.000000	79.9
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	20	20	50.0	50.0	58.1	58.2	0.0	7.9	0.254575	47975	48561	686-687	1.000000	79.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	20	20	50.0	45.0	58.1	57.5	0.0	0.0	0.531462	47975	48561	733-734	1.000000	77.1
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.557071	47975	48561	724-725	1.000000	76.9
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GCCCTGAAATCGAAAATGCC	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.149418	50236	51140	836-1040	1.000000	83.8
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	GCCCTGAAATCGAAAATGCC	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	58.1	58.3	0.0	22.8	0.378799	50236	51140	837-1041	1.000000	81.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCTGGTCCTTCACTAACCG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	57.3	57.9	0.0	0.0	0.732539	50236	51140	865-1069	1.000000	79.1
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTGGTCCTTCACTAACCGG	GGTTCGAATCCGTATAGCCC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.812623	50236	51140	864-1068	1.000000	78.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCACTAACCGGGGCTAAAAG	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.2	57.9	6.3	0.0	0.849972	50236	51140	855-1059	1.000000	78.2
rbcL_p1	rbcL	1	TTGTGGAGGATCTGTGCCTA	GACTCACGATTACGCCTGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056621	54894	56339	2638-2666	1.000000	82.0
rbcL_p2	rbcL	2	TTGTGGAGGATCTGTGCCTA	GTTGACTCACGATTACGCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.056621	54894	56339	2641-2669	1.000000	82.0
rbcL_p3	rbcL	3	TTGTGGAGGATCTGTGCCTA	TTGACTCACGATTACGCCTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057991	54894	56339	2640-2668	1.000000	82.0
rbcL_p4	rbcL	4	GGGATTAATTTGGGTTGCGC	GACTCACGATTACGCCTGTT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	54894	56339	2466-2494	1.000000	82.0
rbcL_p5	rbcL	5	GGGATTAATTTGGGTTGCGC	GTTGACTCACGATTACGCCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	54894	56339	2469-2497	1.000000	82.0
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.493582	62875	64050	1261-1334	1.000000	81.0
psbE-petL_p2	psbE-petL	2	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.0	58.5	0.0	0.0	0.497931	62875	64050	1259-1332	1.000000	81.0
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	62875	64050	1250-1323	1.000000	80.8
psbE-petL_p4	psbE-petL	4	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	62875	64050	1248-1321	1.000000	80.7
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.713535	62875	64050	1252-1325	1.000000	79.3
psaJ-rpl33_p1	psaJ-rpl33	1	TTCCCGGATGCTTTGTCATT	GAAATCCCCGTCGATTCCTC	20	20	45.0	55.0	57.8	58.1	0.7	0.0	0.347210	65358	65811	558-579	1.000000	81.9
psaJ-rpl33_p2	psaJ-rpl33	2	CGGATGCTTTGTCATTCCCT	GAAATCCCCGTCGATTCCTC	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.377654	65358	65811	554-575	1.000000	81.7
psaJ-rpl33_p3	psaJ-rpl33	3	TTCCCGGATGCTTTGTCATT	GGCACCTTTTCGAACACAAC	20	20	45.0	50.0	57.8	58.2	0.7	0.0	0.379255	65358	65811	535-556	1.000000	81.7
psaJ-rpl33_p4	psaJ-rpl33	4	TCCCGGATGCTTTGTCATTC	GAAATCCCCGTCGATTCCTC	20	20	50.0	55.0	58.2	58.1	0.7	0.0	0.382641	65358	65811	557-578	1.000000	81.6
psaJ-rpl33_p5	psaJ-rpl33	5	CGGATGCTTTGTCATTCCCT	GGCACCTTTTCGAACACAAC	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.409700	65358	65811	531-552	1.000000	81.5
ycf15-trnL-CAA_p1	ycf15-trnL-CAA	1	GCCAAAGTCACTGAATGGGT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.4	58.0	6.7	15.9	0.387008	85502	86143	825-825	1.000000	77.2
ycf15-trnL-CAA_p2	ycf15-trnL-CAA	2	TGCCAAAGTCACTGAATGGG	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.4	58.0	6.7	15.9	0.388176	85502	86143	826-826	1.000000	77.2
ycf15-trnL-CAA_p3	ycf15-trnL-CAA	3	GCCAAAGTCACTGAATGGGT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.4	58.0	6.7	15.9	0.388378	85502	86143	827-827	1.000000	77.2
ycf15-trnL-CAA_p4	ycf15-trnL-CAA	4	TGCCAAAGTCACTGAATGGG	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.4	58.0	6.7	15.9	0.389547	85502	86143	828-828	1.000000	77.2
ycf15-trnL-CAA_p5	ycf15-trnL-CAA	5	GCCAAAGTCACTGAATGGGT	TGGTGAAATGGTAGACACGC	20	20	50.0	50.0	58.4	58.2	6.7	0.0	0.575843	85502	86143	830-830	1.000000	75.7
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105003	105879	918-961	1.000000	46.1
ndhF-rpl32_p2	ndhF-rpl32	2	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105003	105879	919-962	1.000000	46.1
ndhF-rpl32_p3	ndhF-rpl32	3	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105003	105879	920-963	1.000000	46.1
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	20	24	55.0	29.2	58.1	55.3	0.0	0.0	6.833076	105003	105879	945-988	1.000000	46.0
ndhF-rpl32_p5	ndhF-rpl32	5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	20	21	55.0	28.6	58.1	52.2	0.0	0.0	6.891734	105003	105879	921-964	1.000000	46.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050149	106072	106776	817-851	1.000000	85.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105116	106072	106776	820-854	1.000000	84.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	106072	106776	789-823	1.000000	84.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	106072	106776	788-822	1.000000	84.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTCCAGCGGTAATGAGCATC	GATCCGCACAAAAGAGTTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077338	106072	106776	1363-1397	1.000000	83.0
ccsA-ndhD_p1	ccsA-ndhD	1	TGGTTCGTTTACATTAACACCT	TTGACAGGGTAGAGGCTCTC	22	20	36.4	55.0	55.9	58.1	0.0	22.5	4.201375	107897	108062	238-238	1.000000	49.1
ccsA-ndhD_p2	ccsA-ndhD	2	TGGTTCGTTTACATTAACACCT	GACAGGGTAGAGGCTCTCTT	22	20	36.4	55.0	55.9	57.8	0.0	22.5	4.208371	107897	108062	236-236	1.000000	49.0
ccsA-ndhD_p3	ccsA-ndhD	3	TGGTTCGTTTACATTAACACCT	CCCTATCCGTTGACAGGGTA	22	20	36.4	55.0	55.9	58.2	0.0	0.0	4.270980	107897	108062	247-247	1.000000	48.4
ccsA-ndhD_p4	ccsA-ndhD	4	TGGTTCGTTTACATTAACACCT	TCTCCCTATCCGTTGACAGG	22	20	36.4	55.0	55.9	58.2	0.0	0.0	4.278676	107897	108062	250-250	1.000000	48.3
ccsA-ndhD_p5	ccsA-ndhD	5	GCACTTATAGCTTCGATAGGA	TTGACAGGGTAGAGGCTCTC	21	20	42.9	55.0	54.7	58.1	0.0	22.5	4.398120	107897	108062	321-321	1.000000	46.8
trnL-CAA-ycf15_p1	trnL-CAA-ycf15	1	AAATGGTAGACACGCGAGAC	GCCAAAGTCACTGAATGGGT	20	20	50.0	50.0	58.0	58.4	15.9	6.7	0.387008	132018	132659	825-825	1.000000	77.2
trnL-CAA-ycf15_p2	trnL-CAA-ycf15	2	AAATGGTAGACACGCGAGAC	TGCCAAAGTCACTGAATGGG	20	20	50.0	50.0	58.0	58.4	15.9	6.7	0.388176	132018	132659	826-826	1.000000	77.2
trnL-CAA-ycf15_p3	trnL-CAA-ycf15	3	TGAAATGGTAGACACGCGAG	GCCAAAGTCACTGAATGGGT	20	20	50.0	50.0	58.0	58.4	15.9	6.7	0.388378	132018	132659	827-827	1.000000	77.2
trnL-CAA-ycf15_p4	trnL-CAA-ycf15	4	TGAAATGGTAGACACGCGAG	TGCCAAAGTCACTGAATGGG	20	20	50.0	50.0	58.0	58.4	15.9	6.7	0.389547	132018	132659	828-828	1.000000	77.2
trnL-CAA-ycf15_p5	trnL-CAA-ycf15	5	TGGTGAAATGGTAGACACGC	GCCAAAGTCACTGAATGGGT	20	20	50.0	50.0	58.2	58.4	0.0	6.7	0.575843	132018	132659	830-830	1.000000	75.7