primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score psbZ-trnG-UCC_p1 psbZ-trnG-UCC 1 CTTCTCCTGATGGTTGGGTG CGTCTTCTCCTTGGCAAAGA 20 20 55.0 50.0 57.9 57.8 0.0 1.1 0.289634 36326 36946 775-851 1.000000 81.8 psbZ-trnG-UCC_p2 psbZ-trnG-UCC 2 TTGCTTCTCCTGATGGTTGG CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 57.8 57.8 0.0 1.1 0.374855 36326 36946 778-854 1.000000 81.1 psbZ-trnG-UCC_p3 psbZ-trnG-UCC 3 TTCTCCTGATGGTTGGGTGA CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 58.3 57.8 0.0 1.1 0.428804 36326 36946 774-850 1.000000 80.7 psbZ-trnG-UCC_p4 psbZ-trnG-UCC 4 CTTCTCCTGATGGTTGGGTG TAAATACATTGCCTGGGCGG 20 20 55.0 50.0 57.9 58.3 0.0 7.1 0.433447 36326 36946 814-890 1.000000 80.4 psbZ-trnG-UCC_p5 psbZ-trnG-UCC 5 TCCTGATGGTTGGGTGAGTA CGTCTTCTCCTTGGCAAAGA 20 20 50.0 50.0 57.7 57.8 0.0 1.1 0.505514 36326 36946 771-847 1.000000 80.1 ndhF_p1 ndhF 1 ACCCCCGTAACTTCTTCGTA ATTGTTGTCGCAGTCCACTT 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.109326 107898 110141 2830-2850 0.500000 60.3 ndhF_p2 ndhF 2 TCGTAACCCCCGTAACTTCT ATTGTTGTCGCAGTCCACTT 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.109326 107898 110141 2835-2855 0.500000 60.3 ndhF_p3 ndhF 3 CAATTCCCGTTCTCAACCCA ATTGTTGTCGCAGTCCACTT 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.128395 107898 110141 2881-2901 0.500000 60.1 ndhF_p4 ndhF 4 GAGGTTTCCTTCGTAACCCC ATTGTTGTCGCAGTCCACTT 20 20 55.0 45.0 57.9 58.0 0.0 0.0 0.137688 107898 110141 2845-2865 0.500000 60.0 ndhF_p5 ndhF 5 ATTCCCGTTCTCAACCCATG ATTGTTGTCGCAGTCCACTT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.161452 107898 110141 2879-2899 0.500000 59.8 ndhF-rpl32_p1 ndhF-rpl32 1 AAAAGCCCACATACGACGAA AAAAAGAATCCCCATCCCGG 20 20 45.0 50.0 57.8 57.8 0.0 0.0 0.332030 110142 110853 1160-1200 0.500000 60.7 ndhF-rpl32_p2 ndhF-rpl32 2 AAAGCCCACATACGACGAAA AAAAAGAATCCCCATCCCGG 20 20 45.0 50.0 57.8 57.8 0.0 0.0 0.332030 110142 110853 1159-1199 0.500000 60.7 ndhF-rpl32_p3 ndhF-rpl32 3 AAGCCCACATACGACGAAAA AAAAAGAATCCCCATCCCGG 20 20 45.0 50.0 57.8 57.8 0.0 0.0 0.332030 110142 110853 1158-1198 0.500000 60.7 ndhF-rpl32_p4 ndhF-rpl32 4 AAGCCCACATACGACGAAAA CAAAGCTTTCAACGCTGTCC 20 20 45.0 50.0 57.8 58.2 0.0 9.2 0.414068 110142 110853 901-932 0.500000 59.9 ndhF-rpl32_p5 ndhF-rpl32 5 GGTCGTGTGAACCAAAAACC AAAAAGAATCCCCATCCCGG 20 20 50.0 50.0 57.8 57.8 0.0 0.0 0.356971 110142 110853 1540-1580 0.500000 59.7 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AAGTGGACTGCGACAACAAT AAAAGGCCCGAAGTAGCAAA 20 20 45.0 45.0 58.0 58.0 0.0 0.0 0.042975 111028 112367 2355-2382 0.500000 61.6 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AAGTGGACTGCGACAACAAT GCTGGTACTAATGTTGCGGA 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.051223 111028 112367 2391-2418 0.500000 61.6 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAGTGGACTGCGACAACAAT AGCTGGTACTAATGTTGCGG 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.056857 111028 112367 2392-2419 0.500000 61.5 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AAGTGGACTGCGACAACAAT AACCATCGGGATTGAAGAGC 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.076804 111028 112367 2409-2436 0.500000 61.4 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AAGTGGACTGCGACAACAAT CGGGATTGAAGAGCTGGTAC 20 20 45.0 55.0 58.0 58.1 0.0 0.0 0.091356 111028 112367 2403-2430 0.500000 61.3 ccsA_p1 ccsA 1 TGGAATTGGTAGACACGCTG ACTATCAGGTGAGAGGGTCG 20 20 50.0 55.0 57.9 57.9 0.0 6.6 0.153356 112567 113532 1459-1459 0.500000 59.4 ccsA_p2 ccsA 2 GTGGAATTGGTAGACACGCT ACTATCAGGTGAGAGGGTCG 20 20 50.0 55.0 57.9 57.9 0.0 6.6 0.154812 112567 113532 1460-1460 0.500000 59.4 ccsA_p3 ccsA 3 TAGACACGCTGCTCTTAGGA ACTATCAGGTGAGAGGGTCG 20 20 50.0 55.0 57.9 57.9 0.0 6.6 0.184327 112567 113532 1450-1450 0.500000 59.2 ccsA_p4 ccsA 4 TGGAATTGGTAGACACGCTG CGGCTTGTTTATCTCGACGA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.189162 112567 113532 1842-1842 0.500000 59.1 ccsA_p5 ccsA 5 GTGGAATTGGTAGACACGCT CGGCTTGTTTATCTCGACGA 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.190618 112567 113532 1843-1843 0.500000 59.0 ccsA-ndhD_p1 ccsA-ndhD 1 TTTGCTACTTCGGGCCTTTT CTCTTTTCCCCTTGGTTGCT 20 20 45.0 50.0 58.0 58.0 0.8 0.0 0.011370 113533 113784 1566-1566 0.500000 60.5 ccsA-ndhD_p2 ccsA-ndhD 2 TTTGCTACTTCGGGCCTTTT TCTCTTTTCCCCTTGGTTGC 20 20 45.0 50.0 58.0 58.0 0.8 0.0 0.014917 113533 113784 1567-1567 0.500000 60.4 ccsA-ndhD_p3 ccsA-ndhD 3 TCCGCAACATTAGTACCAGC CTCTTTTCCCCTTGGTTGCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.019618 113533 113784 1530-1530 0.500000 60.4 ccsA-ndhD_p4 ccsA-ndhD 4 TCCGCAACATTAGTACCAGC TCTCTTTTCCCCTTGGTTGC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.023166 113533 113784 1531-1531 0.500000 60.4 ccsA-ndhD_p5 ccsA-ndhD 5 CCGCAACATTAGTACCAGCT CTCTTTTCCCCTTGGTTGCT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.025252 113533 113784 1529-1529 0.500000 60.4 ndhD_p1 ndhD 1 GCAGTCTGGGCTAATGAGTC TGATCCCTTGGGATGGATGT 20 20 55.0 50.0 58.1 58.1 1.1 24.5 0.141801 113785 115287 2306-2306 0.500000 59.1 ndhD_p2 ndhD 2 GCAGTCTGGGCTAATGAGTC ATGGCATGAAACGACTCGAA 20 20 55.0 45.0 58.1 57.9 1.1 5.2 0.146561 113785 115287 2182-2182 0.500000 59.1 ndhD_p3 ndhD 3 GCAGTCTGGGCTAATGAGTC TGCTTCTGCTCCAAGAACAG 20 20 55.0 50.0 58.1 58.1 1.1 0.0 0.165040 113785 115287 2272-2272 0.500000 59.0 ndhD_p4 ndhD 4 AGTTGGGACCCAAAGGAAAC TGATCCCTTGGGATGGATGT 20 20 50.0 50.0 57.9 58.1 27.9 24.5 0.170686 113785 115287 2270-2270 0.500000 58.9 ndhD_p5 ndhD 5 GTTGGGACCCAAAGGAAACT TGATCCCTTGGGATGGATGT 20 20 50.0 50.0 57.9 58.1 27.9 24.5 0.170686 113785 115287 2269-2269 0.500000 58.9 psaC_p1 psaC 1 CTTCCGCCAGCTGGATTAAT TGGTTAGGGCCCTTATGTGT 20 20 50.0 50.0 58.0 58.3 37.2 34.0 0.350880 115429 115674 1029-1029 0.500000 57.6 psaC_p2 psaC 2 CTTCCGCCAGCTGGATTAAT AGGGCCCTTATGTGTCTTGA 20 20 50.0 50.0 58.0 58.3 37.2 22.3 0.358302 115429 115674 1024-1024 0.500000 57.5 psaC_p3 psaC 3 ACCGTAGAACCCAATACCCA TGGTTAGGGCCCTTATGTGT 20 20 50.0 50.0 58.0 58.3 0.0 34.0 0.368301 115429 115674 1397-1397 0.500000 57.5 psaC_p4 psaC 4 CTTCCGCCAGCTGGATTAAT GGTTAGGGCCCTTATGTGTC 20 20 50.0 55.0 58.0 57.7 37.2 34.0 0.370612 115429 115674 1028-1028 0.500000 57.4 psaC_p5 psaC 5 ACCGTAGAACCCAATACCCA AGGGCCCTTATGTGTCTTGA 20 20 50.0 50.0 58.0 58.3 0.0 22.3 0.375723 115429 115674 1392-1392 0.500000 57.4 ndhE_p1 ndhE 1 TTCGAGTCGTTTCATGCCAT CCGCTGCCCAGTTACTTATT 20 20 45.0 50.0 57.9 58.0 13.1 0.0 0.131693 115891 116196 1389-1389 0.500000 59.5 ndhE_p2 ndhE 2 CTGTTCTTGGAGCAGAAGCA CCGCTGCCCAGTTACTTATT 20 20 50.0 50.0 58.1 58.0 5.7 0.0 0.150172 115891 116196 1299-1299 0.500000 59.4 ndhE_p3 ndhE 3 TCTGTTCTTGGAGCAGAAGC CCGCTGCCCAGTTACTTATT 20 20 50.0 50.0 57.8 58.0 10.7 0.0 0.214265 115891 116196 1300-1300 0.500000 58.9 ndhE_p4 ndhE 4 GGGTTTTGGGGAACGTATCA CCGCTGCCCAGTTACTTATT 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.241857 115891 116196 1426-1426 0.500000 58.6 ndhE_p5 ndhE 5 TTCGAGTCGTTTCATGCCAT TTGCTGTGGCTCGTCAATAA 20 20 45.0 45.0 57.9 57.8 13.1 0.0 0.270700 115891 116196 1053-1053 0.500000 58.5 ndhG_p1 ndhG 1 AGTGCTTGCTGCTGTTAGTT ATTTTGCGGTAACTGCGTTG 20 20 45.0 45.0 58.0 58.0 0.0 4.5 0.072016 116482 117012 1061-1063 0.500000 60.0 ndhG_p2 ndhG 2 TTGAGTGCTTGCTGCTGTTA ATTTTGCGGTAACTGCGTTG 20 20 45.0 45.0 58.0 58.0 0.0 4.5 0.078953 116482 117012 1064-1066 0.500000 59.9 ndhG_p3 ndhG 3 AGTGCTTGCTGCTGTTAGTT ACGACCGTCACGAATTGAAT 20 20 45.0 45.0 58.0 57.9 0.0 0.0 0.101675 116482 117012 987-989 0.500000 59.8 ndhG_p4 ndhG 4 AGTGCTTGCTGCTGTTAGTT TCTACTTACGACCGTCACGA 20 20 45.0 50.0 58.0 57.9 0.0 0.0 0.105976 116482 117012 994-996 0.500000 59.7 ndhG_p5 ndhG 5 TTGAGTGCTTGCTGCTGTTA ACGACCGTCACGAATTGAAT 20 20 45.0 45.0 58.0 57.9 0.0 0.0 0.108612 116482 117012 990-992 0.500000 59.7 ndhG-ndhI_p1 ndhG-ndhI 1 AGTGCTTGCTGCTGTTAGTT CAAGATGGACTTTCCCTCGG 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.056966 117013 117317 1579-1581 0.500000 60.1 ndhG-ndhI_p2 ndhG-ndhI 2 AATAAGTAACTGGGCAGCGG CAAGATGGACTTTCCCTCGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.068225 117013 117317 1206-1208 0.500000 60.1 ndhG-ndhI_p3 ndhG-ndhI 3 TTGAGTGCTTGCTGCTGTTA CAAGATGGACTTTCCCTCGG 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.063904 117013 117317 1582-1584 0.500000 60.0 ndhG-ndhI_p4 ndhG-ndhI 4 AGTGCTTGCTGCTGTTAGTT ATTTTGCGGTAACTGCGTTG 20 20 45.0 45.0 58.0 58.0 0.0 4.5 0.072016 117013 117317 1061-1063 0.500000 60.0 ndhG-ndhI_p5 ndhG-ndhI 5 TTGAGTGCTTGCTGCTGTTA ATTTTGCGGTAACTGCGTTG 20 20 45.0 45.0 58.0 58.0 0.0 4.5 0.078953 117013 117317 1064-1066 0.500000 59.9 ndhI_p1 ndhI 1 AATAAGTAACTGGGCAGCGG CAAGATGGACTTTCCCTCGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.068225 117318 117821 1206-1208 0.500000 60.1 ndhI_p2 ndhI 2 AATAAGTAACTGGGCAGCGG TTCCCTAGCGGAATGTGAGA 20 20 50.0 50.0 58.0 58.1 0.0 8.2 0.177746 117318 117821 1505-1507 0.500000 59.2 ndhI_p3 ndhI 3 AATAAGTAACTGGGCAGCGG AATGCGGATCCGGTCATTAG 20 20 50.0 50.0 58.0 57.8 0.0 34.0 0.208219 117318 117821 1730-1732 0.500000 58.8 ndhI_p4 ndhI 4 AATAAGTAACTGGGCAGCGG TGGGTTTTGGGGTTGGAATT 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.236721 117318 117821 1565-1567 0.500000 58.7 ndhI_p5 ndhI 5 AATAAGTAACTGGGCAGCGG ATTTGTGGCGTCAACCCATA 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.246519 117318 117821 1548-1550 0.500000 58.6 ndhA_p1 ndhA 1 ATTCAATTCGTGACGGTCGT AGAAAGGGCTGCAAAATGGA 20 20 45.0 45.0 57.9 58.0 4.7 0.5 0.088557 117904 120067 2941-2947 0.500000 59.9 ndhA_p2 ndhA 2 TCGTGACGGTCGTAAGTAGA AGAAAGGGCTGCAAAATGGA 20 20 50.0 45.0 57.9 58.0 4.7 0.5 0.092858 117904 120067 2934-2940 0.500000 59.9 ndhA_p3 ndhA 3 TTGACCAATGTACCTCGCTG AGAAAGGGCTGCAAAATGGA 20 20 50.0 45.0 57.9 58.0 0.0 0.5 0.107316 117904 120067 2605-2611 0.500000 59.8 ndhA_p4 ndhA 4 CGGCCCAAAGCAATTTGATT AGAAAGGGCTGCAAAATGGA 20 20 45.0 45.0 57.9 58.0 0.0 0.5 0.111689 117904 120067 2964-2970 0.500000 59.8 ndhA_p5 ndhA 5 ATTCAATTCGTGACGGTCGT AGGGCTGCAAAATGGAATGA 20 20 45.0 45.0 57.9 58.1 4.7 0.5 0.133170 117904 120067 2937-2943 0.500000 59.6 ndhH_p1 ndhH 1 AGCTCGCAAACCACCTAAAA AACGAAGAAAACAGGGGGTC 20 20 45.0 50.0 58.0 58.0 0.0 0.0 0.047284 120069 121250 2009-2009 0.500000 60.1 ndhH_p2 ndhH 2 CAGTTTTGTCCCATCCGCTA AACGAAGAAAACAGGGGGTC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.130894 120069 121250 1763-1763 0.500000 59.5 ndhH_p3 ndhH 3 AGCTCGCAAACCACCTAAAA CCATTTCTTTGTAGCCCGGT 20 20 45.0 50.0 58.0 58.2 0.0 0.0 0.190152 120069 121250 2237-2251 0.500000 59.4 ndhH_p4 ndhH 4 CCATCCGCTAGAGCTTGAAG AACGAAGAAAACAGGGGGTC 20 20 55.0 50.0 58.1 58.0 0.0 0.0 0.159677 120069 121250 1753-1753 0.500000 59.2 ndhH_p5 ndhH 5 GCGTATTCAGGACCAGTACG AACGAAGAAAACAGGGGGTC 20 20 55.0 50.0 58.2 58.0 11.3 0.0 0.186560 120069 121250 1717-1717 0.500000 59.0 rps15_p1 rps15 1 TAGAAAAAGGGAGTCTGCGC ACCATTTCTTTGTAGCCCGG 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.260574 121354 121626 961-975 0.500000 59.7 rps15_p2 rps15 2 TAGAAAAAGGGAGTCTGCGC CCATTTCTTTGTAGCCCGGT 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.260574 121354 121626 960-974 0.500000 59.7 rps15_p3 rps15 3 TCCGCCATAAAAGGTCCAAG ACCATTTCTTTGTAGCCCGG 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.288595 121354 121626 934-948 0.500000 59.4 rps15_p4 rps15 4 TCCGCCATAAAAGGTCCAAG CCATTTCTTTGTAGCCCGGT 20 20 50.0 50.0 57.9 58.2 0.0 0.0 0.288595 121354 121626 933-947 0.500000 59.4 rps15_p5 rps15 5 TAGAAAAAGGGAGTCTGCGC GTAACCGTCTTGCTAGCGAT 20 20 50.0 50.0 57.9 57.8 0.0 10.8 0.300069 121354 121626 1332-1346 0.500000 59.0 rps15-trnN-GUU_p1 rps15-trnN-GUU 1 CCAAAAGTTGCCAAACTGGG TTTTGCGGGACAGCTCATAA 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.240471 126600 126719 1116-1200 0.500000 62.8 rps15-trnN-GUU_p2 rps15-trnN-GUU 2 CCAAAAGTTGCCAAACTGGG TTTTTGCGGGACAGCTCATA 20 20 50.0 45.0 58.0 57.8 0.0 0.0 0.240471 126600 126719 1117-1201 0.500000 62.8 rps15-trnN-GUU_p3 rps15-trnN-GUU 3 ATGGTCGTCTCCTTCGGTTA TTTTGCGGGACAGCTCATAA 20 20 50.0 45.0 58.2 57.8 0.0 0.0 0.349295 126600 126719 983-1067 0.500000 62.0 rps15-trnN-GUU_p4 rps15-trnN-GUU 4 TGGTCGTCTCCTTCGGTTAT TTTTGCGGGACAGCTCATAA 20 20 50.0 45.0 58.2 57.8 0.0 0.0 0.349295 126600 126719 982-1066 0.500000 62.0 rps15-trnN-GUU_p5 rps15-trnN-GUU 5 TGGTCGTCTCCTTCGGTTAT TTTTTGCGGGACAGCTCATA 20 20 50.0 45.0 58.2 57.8 0.0 0.0 0.349295 126600 126719 983-1067 0.500000 62.0