primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	69	871	830-869	1.000000	83.6
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	69	871	844-883	1.000000	83.5
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	69	871	843-882	1.000000	83.5
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	69	871	845-884	1.000000	83.5
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	69	871	833-872	1.000000	83.5
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	CATCGTTAGCTTGGAAGGCT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.068045	10013	12645	2783-2946	1.000000	80.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	GTGGAGAGAGAGGGATTCGA	CATCGTTAGCTTGGAAGGCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.089888	10013	12645	2832-2995	1.000000	80.5
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ATTAGCAATCCGCCGCTTTA	CGTTAGCTTGGAAGGCTAGG	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.096882	10013	12645	2780-2943	1.000000	80.5
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AGTGGAGAGAGAGGGATTCG	CATCGTTAGCTTGGAAGGCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.095737	10013	12645	2833-2996	1.000000	80.4
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GTGGAGAGAGAGGGATTCGA	CGTTAGCTTGGAAGGCTAGG	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.118725	10013	12645	2829-2992	1.000000	80.3
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	ATCAACCAAAAAGGGGAGGG	20	20	45.0	50.0	57.4	57.7	9.6	0.0	0.967799	14757	16147	1565-1576	1.000000	73.2
atpF_p2	atpF	2	ACTAATTTCGTCGGCTCGAA	GGAGGGCGAAGTGATACAAA	20	20	45.0	50.0	57.4	57.6	9.6	0.0	1.038728	14757	16147	1551-1562	1.000000	72.6
atpF_p3	atpF	3	AATTTCGTCGGCTCGAATGG	ATCAACCAAAAAGGGGAGGG	20	20	50.0	50.0	59.0	57.7	13.2	0.0	1.318829	14757	16147	1562-1573	1.000000	70.4
atpF_p4	atpF	4	AATTTCGTCGGCTCGAATGG	GGAGGGCGAAGTGATACAAA	20	20	50.0	50.0	59.0	57.6	13.2	0.0	1.389757	14757	16147	1548-1559	1.000000	69.8
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAAT	ATCAACCAAAAAGGGGAGGG	21	20	42.9	50.0	57.8	57.7	13.2	0.0	1.486438	14757	16147	1565-1576	1.000000	69.0
rpoC2_p1	rpoC2	1	GTTCCGCTCTCAACAATCCT	ACGCAAGGTCTTCCAAAAGT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.153387	20202	20321	340-340	1.000000	79.2
rpoC2_p2	rpoC2	2	CTCGTTCCGCTCTCAACAAT	ACGCAAGGTCTTCCAAAAGT	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.337988	20202	20321	343-343	1.000000	77.8
rpoC2_p3	rpoC2	3	TCGTTCCGCTCTCAACAATC	ACGCAAGGTCTTCCAAAAGT	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.342769	20202	20321	342-342	1.000000	77.7
rpoC2_p4	rpoC2	4	GTTCCGCTCTCAACAATCCT	CGCAAGGTCTTCCAAAAGTG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.506497	20202	20321	339-339	1.000000	76.4
rpoC2_p5	rpoC2	5	CTCGTTCCGCTCTCAACAAT	CGCAAGGTCTTCCAAAAGTG	20	20	50.0	50.0	58.3	57.6	0.0	0.0	0.691098	20202	20321	342-342	1.000000	74.9
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.315353	34112	34717	717-719	1.000000	61.8
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAACAGTGCAGTAGC	CCGCTGGGATTGTAGTTCAA	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.337248	34112	34717	730-732	1.000000	61.6
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGAATGAACAGTGCAGTAGC	ATGTATGCTGTACATCCCGC	20	20	45.0	50.0	55.8	57.8	0.0	0.0	2.399131	34112	34717	746-748	1.000000	61.1
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	22	20	40.9	50.0	57.4	57.9	0.0	0.0	2.709987	34112	34717	724-726	1.000000	58.7
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	GTATGCTGTACATCCCGCTG	20	20	45.0	55.0	55.8	58.5	0.0	0.0	2.727067	34112	34717	744-746	1.000000	58.5
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TTGCTTCTTCCGATGGATGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101361	40735	41324	755-758	1.000000	80.2
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTTCCGATGGATGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101361	40735	41324	756-759	1.000000	80.2
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTGCTTCTTCCGATGGATGG	TACTTCACCATGGGCGGATA	20	20	50.0	50.0	58.0	58.2	0.0	18.1	0.246669	40735	41324	780-783	1.000000	79.1
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TTGCTTCTTCCGATGGATGG	ACATTACTTCACCATGGGCG	20	20	50.0	50.0	58.0	58.2	0.0	18.1	0.292493	40735	41324	784-787	1.000000	78.7
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	GCTTCTTCCGATGGATGGTC	AATCGAACCCGCATCTTCTC	20	20	55.0	50.0	58.4	58.1	0.0	0.0	0.471128	40735	41324	753-756	1.000000	77.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGGCTACAACTGAAGAGGTC	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	56.9	58.1	0.0	0.0	1.224897	49258	50155	1017-1023	1.000000	70.7
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TGGCTACAACTGAAGAGGTC	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	56.9	58.2	0.0	0.0	1.281883	49258	50155	1038-1044	1.000000	70.3
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TGGCTACAACTGAAGAGGTC	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	56.9	57.8	0.0	5.6	1.323198	49258	50155	988-994	1.000000	70.0
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TGGCTACAACTGAAGAGGTCT	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.4	58.1	0.0	0.0	1.484616	49258	50155	1017-1023	1.000000	68.7
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTGGCTACAACTGAAGAGGTC	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	57.6	58.1	0.0	0.0	1.496990	49258	50155	1018-1024	1.000000	68.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GGAAAAAGCCAGCTATCGGA	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043011	51897	53175	1378-1627	1.000000	82.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AGGGAAAAAGCCAGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048746	51897	53175	1380-1629	1.000000	82.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAAAAAGCCAGCTATCGGA	TCCATACCAAGGCTCAATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.095524	51897	53175	1417-1666	1.000000	81.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AGGGAAAAAGCCAGCTATCG	TCCATACCAAGGCTCAATGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.101260	51897	53175	1419-1668	1.000000	81.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAAAAAGCCAGCTATCGGA	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.107820	51897	53175	1386-1635	1.000000	81.6
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	54224	55203	1079-1090	1.000000	80.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AAGATAAGGCGATGGCATGG	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	3.8	0.0	0.227180	54224	55203	1144-1155	1.000000	80.0
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	GATAAGGCGATGGCATGGAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	14.1	0.0	0.232551	54224	55203	1142-1153	1.000000	80.0
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	TAAGGCGATGGCATGGAAAA	TGAAAGTTGGATAGGCTGGC	20	20	45.0	50.0	57.9	57.9	14.1	0.0	0.265069	54224	55203	1140-1151	1.000000	79.7
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.334056	54224	55203	1121-1132	1.000000	79.2
accD-psaI_p1	accD-psaI	1	TCACAAGCGGCTGAGTATTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	1.8	26.9	0.037148	65584	66945	1545-1552	1.000000	81.1
accD-psaI_p2	accD-psaI	2	CCACGGTTTCCTTTCCTTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	65584	66945	1450-1457	1.000000	81.1
accD-psaI_p3	accD-psaI	3	TCCACGGTTTCCTTTCCTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.051344	65584	66945	1451-1458	1.000000	81.1
accD-psaI_p4	accD-psaI	4	GCGGCTGAGTATTCATTCCA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.073444	65584	66945	1539-1546	1.000000	80.8
accD-psaI_p5	accD-psaI	5	TCACAAGCGGCTGAGTATTC	AGAAACCATTGCAATTGCCG	20	20	50.0	45.0	58.0	57.9	1.8	26.9	0.093312	65584	66945	1550-1557	1.000000	80.6
ycf4-cemA_p1	ycf4-cemA	1	TTAGCCTATTTCTTGCGCGT	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	58.0	57.8	8.4	0.0	0.176182	67979	68978	1100-1101	1.000000	78.9
ycf4-cemA_p2	ycf4-cemA	2	TGCGCGTACCAATTGAAGTA	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	57.9	57.8	8.4	0.0	0.239365	67979	68978	1087-1088	1.000000	78.4
ycf4-cemA_p3	ycf4-cemA	3	CTTGCGCGTACCAATTGAAG	ATGAGAGAGAGACCCACCAG	20	20	50.0	55.0	58.1	57.8	8.4	0.0	0.275442	67979	68978	1089-1090	1.000000	78.1
ycf4-cemA_p4	ycf4-cemA	4	AAAAGCTGCGGAATTAGCCT	ATGAGAGAGAGACCCACCAG	20	20	45.0	55.0	58.2	57.8	0.0	0.0	0.318216	67979	68978	1113-1114	1.000000	77.7
ycf4-cemA_p5	ycf4-cemA	5	TTAGCCTATTTCTTGCGCGT	TTCGGATTGCCTGGTATTCC	20	20	45.0	50.0	58.0	57.7	8.4	13.7	0.360388	67979	68978	1156-1157	1.000000	77.4
petA-psbJ_p1	petA-psbJ	1	CGCGTTCAAGGTCTTTTGTT	TCCGGATTAGGCTCATCTCT	20	20	45.0	50.0	57.5	57.3	0.0	0.0	1.153638	70845	72024	1309-1315	1.000000	72.1
petA-psbJ_p2	petA-psbJ	2	CGCGTTCAAGGTCTTTTGTT	CCGGATTAGGCTCATCTCTG	20	20	45.0	55.0	57.5	57.3	0.0	8.5	1.208417	70845	72024	1308-1314	1.000000	71.7
petA-psbJ_p3	petA-psbJ	3	ACGCGTTCAAGGTCTTTTGT	TCCGGATTAGGCTCATCTCT	20	20	45.0	50.0	58.6	57.3	5.6	0.0	1.309555	70845	72024	1310-1316	1.000000	70.9
petA-psbJ_p4	petA-psbJ	4	ACGCGTTCAAGGTCTTTTGT	CCGGATTAGGCTCATCTCTG	20	20	45.0	55.0	58.6	57.3	5.6	8.5	1.364334	70845	72024	1309-1315	1.000000	70.4
petA-psbJ_p5	petA-psbJ	5	CGCGTTCAAGGTCTTTTGTT	TTCCGGATTAGGCTCATCTCT	20	21	45.0	47.6	57.5	58.0	0.0	4.1	1.504322	70845	72024	1310-1316	1.000000	69.3
psbT-psbN_p1	psbT-psbN	1	CCTCCTAAGGTTCCGACTAA	GAGACCCATTCGAGGAACAC	20	20	50.0	55.0	55.7	58.0	0.0	0.0	2.336032	82497	82570	136-136	1.000000	61.9
psbT-psbN_p2	psbT-psbN	2	CCTCCTAAGGTTCCGACTAA	TCGCCATCTCCATATCTGGT	20	20	50.0	50.0	55.7	58.0	0.0	0.0	2.339949	82497	82570	220-220	1.000000	61.6
psbT-psbN_p3	psbT-psbN	3	CCTCCTAAGGTTCCGACTAA	AGAGACCCATTCGAGGAACA	20	20	50.0	50.0	55.7	58.1	0.0	0.0	2.385908	82497	82570	137-137	1.000000	61.5
psbT-psbN_p4	psbT-psbN	4	CCTCCTAAGGTTCCGACTAAA	GAGACCCATTCGAGGAACAC	21	20	47.6	55.0	56.5	58.0	0.0	0.0	2.544810	82497	82570	136-136	1.000000	60.2
psbT-psbN_p5	psbT-psbN	5	CCTCCTAAGGTTCCGACTAA	ACCTTAGTCGCCATCTCCAT	20	20	50.0	50.0	55.7	58.2	0.0	0.0	2.529778	82497	82570	227-227	1.000000	60.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTTGGCTGCGCTAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.4	58.0	9.7	0.0	0.403227	122592	123231	775-783	1.000000	77.1
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGATATTTGGCTGCGCTAA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.4	58.0	9.7	0.0	0.404597	122592	123231	776-784	1.000000	77.1
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGATATTTGGCTGCGCTAA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.4	57.9	9.7	0.0	0.519673	122592	123231	785-793	1.000000	76.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGATATTTGGCTGCGCTAA	CCTAAGAGCAGCGTGTCTAC	20	20	50.0	55.0	58.4	57.8	9.7	0.0	0.591691	122592	123231	784-792	1.000000	75.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGATATTTGGCTGCGCTAA	CTAAGAGCAGCGTGTCTACC	20	20	50.0	55.0	58.4	57.8	9.7	0.0	0.591691	122592	123231	783-791	1.000000	75.6
psaC-ndhE_p1	psaC-ndhE	1	AAAACATCTGTAGGGCAGGC	CGTAACAGAAAATCAACGCGT	20	21	50.0	42.9	58.2	57.8	0.0	5.6	1.324219	126456	127090	730-763	1.000000	72.9
psaC-ndhE_p2	psaC-ndhE	2	AAAACATCTGTAGGGCAGGC	GCCGAAGCAGCTATTGGTTT	20	20	50.0	50.0	58.2	59.2	0.0	0.0	1.349295	126456	127090	772-805	1.000000	72.6
psaC-ndhE_p3	psaC-ndhE	3	AAAACATCTGTAGGGCAGGC	CGAAGCAGCTATTGGTTTAGC	20	21	50.0	47.6	58.2	57.7	0.0	0.0	1.485806	126456	127090	770-803	1.000000	71.5
psaC-ndhE_p4	psaC-ndhE	4	TGCTTAGCTTTACATCCCGG	CGTAACAGAAAATCAACGCGT	20	21	50.0	42.9	57.7	57.8	5.6	5.6	1.489225	126456	127090	766-799	1.000000	71.5
psaC-ndhE_p5	psaC-ndhE	5	TGCTTAGCTTTACATCCCGG	GCCGAAGCAGCTATTGGTTT	20	20	50.0	50.0	57.7	59.2	5.6	0.0	1.514302	126456	127090	808-841	1.000000	71.1
ndhG-ndhI_p1	ndhG-ndhI	1	CTACCCCTAAGCCCCCTAAT	TCTTCATTCAAGGACGATTACCA	20	23	55.0	39.1	57.6	57.6	0.0	0.0	3.849992	128305	128998	801-1006	1.000000	51.1
ndhG-ndhI_p2	ndhG-ndhI	2	CTACCCCTAAGCCCCCTAAT	ACTTCTTCATTCAAGGACGA	20	20	55.0	40.0	57.6	54.3	0.0	0.0	4.111697	128305	128998	804-1009	1.000000	49.0
ndhG-ndhI_p3	ndhG-ndhI	3	CTACCCCTAAGCCCCCTAAT	CTTCATTCAAGGACGATTACCA	20	22	55.0	40.9	57.6	56.2	0.0	0.0	4.259753	128305	128998	800-1005	1.000000	47.9
ndhG-ndhI_p4	ndhG-ndhI	4	CTACCCCTAAGCCCCCTAAT	AACTTCTTCATTCAAGGACGA	20	21	55.0	38.1	57.6	55.2	0.0	0.0	4.260195	128305	128998	805-1010	1.000000	47.9
ndhG-ndhI_p5	ndhG-ndhI	5	CTACCCCTAAGCCCCCTAAT	TCATTCAAGGACGATTACCA	20	20	55.0	40.0	57.6	54.1	0.0	0.0	4.356368	128305	128998	798-1003	1.000000	47.1
rps15-ycf1_p1	rps15-ycf1	1	TCAACAGACCCTTTGTTTTCT	TGGTAGTCGTTTCAGTATGTCA	21	22	38.1	40.9	55.5	56.9	0.0	0.0	6.580829	133411	134369	1106-1309	1.000000	43.9
rps15-ycf1_p2	rps15-ycf1	2	TCAACAGACCCTTTGTTTTCT	AGCTTGTATGAATCGGTATTGGT	21	23	38.1	39.1	55.5	58.0	0.0	0.0	6.493892	133411	134369	1142-1345	1.000000	43.8
rps15-ycf1_p3	rps15-ycf1	3	TCAACAGACCCTTTGTTTTCTTC	AGCTTGTATGAATCGGTATTGGT	23	23	39.1	39.1	57.4	58.0	0.0	0.0	6.612564	133411	134369	1142-1345	1.000000	43.8
rps15-ycf1_p4	rps15-ycf1	4	CAACAGACCCTTTGTTTTCTTCT	AGCTTGTATGAATCGGTATTGGT	23	23	39.1	39.1	57.4	58.0	0.0	0.0	6.618806	133411	134369	1141-1344	1.000000	43.8
rps15-ycf1_p5	rps15-ycf1	5	TGAAATTCAACAGACCCTTTGT	AGCTTGTATGAATCGGTATTGGT	22	23	36.4	39.1	56.4	58.0	0.0	0.0	6.662901	133411	134369	1148-1351	1.000000	43.8