primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCCTGAAGGAGTAGCGGATA	TCCGGGTTGCTAACTCAATG	20	20	50.0	50.0	57.0	57.9	0.0	0.0	1.152416	3264	4393	1171-1306	1.000000	79.4
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCCTGAAGGAGTAGCGGATA	CGGGTTGCTAACTCAATGGT	20	20	50.0	50.0	57.0	58.2	0.0	0.0	1.223250	3264	4393	1169-1304	1.000000	78.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	TCCTGAAGGAGTAGCGGATA	GATCCGGGTTGCTAACTCAA	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.441056	3264	4393	1173-1308	1.000000	77.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	TCCTGAAGGAGTAGCGGATA	TTGATCCGGGTTGCTAACTC	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.441056	3264	4393	1175-1310	1.000000	77.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCCTGAAGGAGTAGCGGATAT	TCCGGGTTGCTAACTCAATG	21	20	47.6	50.0	57.5	57.9	0.0	0.0	1.635457	3264	4393	1171-1306	1.000000	75.5
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7567	8059	602-629	1.000000	86.8
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	20	20	50.0	50.0	57.9	58.1	1.4	0.7	0.123073	7567	8059	603-630	1.000000	86.7
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	TTACGCCCCGGATCATTAGA	20	20	50.0	50.0	57.9	58.3	1.4	0.7	0.348988	7567	8059	604-631	1.000000	84.9
psbK-psbI_p4	psbK-psbI	4	TGGCAAGCTGCTGTAAGTTT	ACGCCCCGGATCATTAGATA	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	7567	8059	599-626	1.000000	84.6
psbK-psbI_p5	psbK-psbI	5	TGGCAAGCTGCTGTAAGTTT	TACGCCCCGGATCATTAGAT	20	20	45.0	50.0	58.3	58.1	1.4	0.7	0.375782	7567	8059	600-627	1.000000	84.6
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8429	9717	716-1357	1.000000	63.7
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8429	9717	715-1356	1.000000	63.7
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	CAATCCGACGCTTTAGTCCA	AGAACGAATCACACTTTTACCAC	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8429	9717	719-1360	1.000000	62.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.673141	8429	9717	718-1359	1.000000	62.6
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	20	23	50.0	39.1	58.0	57.3	0.0	0.0	3.674529	8429	9717	717-1358	1.000000	62.6
atpH-atpI_p1	atpH-atpI	1	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	20	20	50.0	45.0	58.1	57.9	0.0	18.8	0.186851	14370	15017	698-830	1.000000	85.4
atpH-atpI_p2	atpH-atpI	2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	20	20	50.0	45.0	57.9	57.9	0.0	18.8	0.204923	14370	15017	647-779	1.000000	85.3
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	20	20	45.0	45.0	57.9	57.9	0.0	18.8	0.271229	14370	15017	626-758	1.000000	84.9
atpH-atpI_p4	atpH-atpI	4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	20	20	55.0	45.0	57.8	57.9	0.0	18.8	0.279126	14370	15017	646-778	1.000000	84.7
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	20	20	50.0	45.0	57.8	57.9	0.0	18.8	0.337170	14370	15017	625-757	1.000000	84.3
petN-psbM_p1	petN-psbM	1	GGGGCATCATTCACATGGAT	ACGTCAAAACAGTCAGCCAA	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.260138	29921	30757	938-970	1.000000	85.5
petN-psbM_p2	petN-psbM	2	GGGGCATCATTCACATGGAT	ATTGCTACTGCGCTGTTCAT	20	20	50.0	45.0	58.0	58.3	0.0	25.3	0.262609	29921	30757	990-1022	1.000000	85.3
petN-psbM_p3	petN-psbM	3	GGGGCATCATTCACATGGAT	TATTGCTACTGCGCTGTTCA	20	20	50.0	45.0	58.0	57.6	0.0	25.3	0.383815	29921	30757	991-1023	1.000000	84.3
petN-psbM_p4	petN-psbM	4	GGATAGGGGGCATCATTCAC	ACGTCAAAACAGTCAGCCAA	20	20	55.0	45.0	57.8	58.3	0.0	0.0	0.461344	29921	30757	944-976	0.923077	80.6
petN-psbM_p5	petN-psbM	5	GGATAGGGGGCATCATTCAC	ATTGCTACTGCGCTGTTCAT	20	20	55.0	45.0	57.8	58.3	0.0	25.3	0.463815	29921	30757	996-1028	0.923077	80.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	32617	33422	864-974	1.000000	86.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.8	58.1	0.0	0.0	0.233900	32617	33422	843-953	1.000000	86.6
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.256054	32617	33422	840-950	1.000000	86.4
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	32617	33422	842-952	1.000000	86.1
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	32617	33422	861-971	1.000000	85.8
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GGCGTAAGTCATCGGTTCAA	TCTACCAAAGGCTATAGTCA	20	20	50.0	40.0	58.3	52.4	0.0	7.2	5.845447	33495	34530	1042-1102	1.000000	47.9
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GTGGTAGAGTAACGCCATGG	TCTACCAAAGGCTATAGTCA	20	20	55.0	40.0	58.1	52.4	7.6	7.2	5.626709	33495	34530	1065-1125	1.000000	47.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	TCAGTGGTAGAGTAACGCCA	TCTACCAAAGGCTATAGTCA	20	20	50.0	40.0	58.1	52.4	0.0	7.2	5.648863	33495	34530	1068-1128	1.000000	47.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	TCTACCAAAGGCTATAGTCAT	20	21	55.0	38.1	58.1	53.2	7.6	7.2	5.905431	33495	34530	1065-1125	1.000000	47.8
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCCCTTTTAACTCAGTGGT	TCTACCAAAGGCTATAGTCA	20	20	50.0	40.0	57.7	52.4	0.0	7.2	5.850619	33495	34530	1080-1140	1.000000	47.7
trnL-UAA_p1	trnL-UAA	1	ACAGCTGGATTCTTGTCTCA	AGCGAGGAAGTAAAATGGGC	20	20	45.0	50.0	56.5	58.2	19.9	0.0	1.780530	48428	49236	571-880	0.923077	74.0
trnL-UAA_p2	trnL-UAA	2	ACAGCTGGATTCTTGTCTCA	GCGAGGAAGTAAAATGGGCT	20	20	45.0	50.0	56.5	58.2	19.9	0.0	1.780530	48428	49236	570-879	0.923077	74.0
trnL-UAA_p3	trnL-UAA	3	ACAGCTGGATTCTTGTCTCAA	AGCGAGGAAGTAAAATGGGC	21	20	42.9	50.0	57.2	58.2	19.9	0.0	2.025669	48428	49236	571-880	0.923077	72.0
trnL-UAA_p4	trnL-UAA	4	ACAGCTGGATTCTTGTCTCAA	GCGAGGAAGTAAAATGGGCT	21	20	42.9	50.0	57.2	58.2	19.9	0.0	2.025669	48428	49236	570-879	0.923077	72.0
trnL-UAA_p5	trnL-UAA	5	ACAGCTGGATTCTTGTCTCAAA	GCGAGGAAGTAAAATGGGCT	22	20	40.9	50.0	57.9	58.2	19.9	0.0	2.339324	48428	49236	570-879	0.923077	69.5
trnF-GAA-trnV-UAC_p1	trnF-GAA-trnV-UAC	1	CCTCGTGTCACCAGTTCAAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.249179	49697	50372	677-2176	1.000000	90.0
trnF-GAA-trnV-UAC_p2	trnF-GAA-trnV-UAC	2	GGAAATGGTCGGGATAGCTC	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	57.8	57.8	0.0	0.0	0.368624	49697	50372	723-2222	1.000000	89.1
trnF-GAA-trnV-UAC_p3	trnF-GAA-trnV-UAC	3	AATGGTCGGGATAGCTCAGT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.402569	49697	50372	720-2219	1.000000	88.8
trnF-GAA-trnV-UAC_p4	trnF-GAA-trnV-UAC	4	ATGGTCGGGATAGCTCAGTT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.2	57.8	0.0	0.0	0.402569	49697	50372	719-2218	1.000000	88.8
trnF-GAA-trnV-UAC_p5	trnF-GAA-trnV-UAC	5	CTCTGCTAGGATGATGCACG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.416477	49697	50372	743-2242	1.000000	88.7
atpB-rbcL_p1	atpB-rbcL	1	ATCTTGCCCGGTGGAAAAAT	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	58.1	58.1	0.0	0.0	0.181868	53388	54388	1096-1200	1.000000	85.5
atpB-rbcL_p2	atpB-rbcL	2	CGCCCTTGGTTTTGTTCTTC	GGTGACATAAGTCCCTCCCT	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.253261	53388	54388	1042-1146	1.000000	85.0
atpB-rbcL_p3	atpB-rbcL	3	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	57.9	58.1	2.1	0.0	0.255358	53388	54388	1064-1168	1.000000	85.0
atpB-rbcL_p4	atpB-rbcL	4	TACGCCCTTGGTTTTGTTCT	GGTGACATAAGTCCCTCCCT	20	20	45.0	55.0	57.6	58.1	0.0	0.0	0.470765	53388	54388	1044-1148	1.000000	83.2
atpB-rbcL_p5	atpB-rbcL	5	ATCTTGCCCGGTGGAAAAAT	TGGTGACATAAGTCCCTCCC	20	20	45.0	55.0	58.1	58.4	0.0	0.0	0.482749	53388	54388	1097-1201	1.000000	83.1
clpP-psbB_p1	clpP-psbB	1	CGCACTATACGTCAACCCAA	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028080	71457	72200	804-1050	1.000000	91.8
clpP-psbB_p2	clpP-psbB	2	CTTTTGGAACACCAATGGGC	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	57.8	58.0	3.6	0.0	0.195454	71457	72200	750-996	1.000000	90.4
clpP-psbB_p3	clpP-psbB	3	ACCCAAACTGCATCTTCCTC	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.233704	71457	72200	790-1036	1.000000	90.1
clpP-psbB_p4	clpP-psbB	4	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.238244	71457	72200	726-972	1.000000	90.1
clpP-psbB_p5	clpP-psbB	5	TCCTCTCCAGGATTCCGAAA	CAACCAGAAACCAGAGCTGT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.274827	71457	72200	775-1021	1.000000	89.8
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	CCTTCGAAGAACATGGGGAC	21	20	47.6	55.0	57.6	58.3	0.0	26.6	1.657194	74188	74249	124-124	1.000000	72.5
psbT-psbN_p2	psbT-psbN	2	CGAGAACCACCTAAAGTTCCA	CCCTAGTCGCCATCTCCATA	21	20	47.6	55.0	57.6	58.1	0.0	0.0	1.471529	74188	74249	220-220	1.000000	71.5
psbT-psbN_p3	psbT-psbN	3	TCGAGAACCACCTAAAGTTCC	CCTTCGAAGAACATGGGGAC	21	20	47.6	55.0	57.3	58.3	0.0	26.6	1.933700	74188	74249	125-125	1.000000	70.3
psbT-psbN_p4	psbT-psbN	4	TCGAGAACCACCTAAAGTTCC	CCCTAGTCGCCATCTCCATA	21	20	47.6	55.0	57.3	58.1	0.0	0.0	1.748036	74188	74249	221-221	1.000000	69.3
psbT-psbN_p5	psbT-psbN	5	CGAGAACCACCTAAAGTTCCA	GCCATCTCCATATCCGGTTT	21	20	47.6	50.0	57.6	57.4	0.0	0.0	1.964883	74188	74249	212-212	1.000000	67.7
rpl16_p1	rpl16	1	ATTGGGCACTGCTTCTTTGA	GCAAATTGCAGGACGTATCG	20	20	45.0	50.0	58.0	57.9	0.0	13.7	0.091503	81335	83045	2138-2328	1.000000	86.3
rpl16_p2	rpl16	2	TGGGCACTGCTTCTTTGATT	GCAAATTGCAGGACGTATCG	20	20	45.0	50.0	58.0	57.9	0.0	13.7	0.091503	81335	83045	2136-2326	1.000000	86.3
rpl16_p3	rpl16	3	TTGGGCACTGCTTCTTTGAT	GCAAATTGCAGGACGTATCG	20	20	45.0	50.0	58.0	57.9	0.0	13.7	0.091503	81335	83045	2137-2327	1.000000	86.3
rpl16_p4	rpl16	4	TCGCCAAGATGAGCATATCG	GCAAATTGCAGGACGTATCG	20	20	50.0	50.0	57.9	57.9	0.0	13.7	0.161413	81335	83045	2101-2291	1.000000	85.8
rpl16_p5	rpl16	5	TATCATGCCGTTGTCACGTT	GCAAATTGCAGGACGTATCG	20	20	45.0	50.0	57.9	57.9	0.0	13.7	0.178653	81335	83045	2219-2409	1.000000	85.5
trnN-GUU-rpl32_p1	trnN-GUU-rpl32	1	AGTCAGTTAACAGCCAACCG	GCTTTTACTGCCTCTAAAGATCC	20	23	50.0	43.5	58.1	57.3	8.6	0.0	3.864073	109673	110425	783-943	1.000000	60.9
trnN-GUU-rpl32_p2	trnN-GUU-rpl32	2	ACGACCAGTCAGTTAACAGC	GCTTTTACTGCCTCTAAAGATCC	20	23	50.0	43.5	57.8	57.3	8.6	0.0	3.893423	109673	110425	789-949	1.000000	60.6
trnN-GUU-rpl32_p3	trnN-GUU-rpl32	3	AGGATTCGAACCTACGACCA	GCTTTTACTGCCTCTAAAGATCC	20	23	50.0	43.5	58.2	57.3	15.0	0.0	3.899658	109673	110425	802-962	1.000000	60.5
trnN-GUU-rpl32_p4	trnN-GUU-rpl32	4	AGTCAGTTAACAGCCAACCG	AGAAAAAGCTTTTACTGCCTCT	20	22	50.0	36.4	58.1	56.2	8.6	24.1	3.939883	109673	110425	790-950	1.000000	60.2
trnN-GUU-rpl32_p5	trnN-GUU-rpl32	5	GGATTCGAACCTACGACCAG	GCTTTTACTGCCTCTAAAGATCC	20	23	55.0	43.5	57.8	57.3	0.0	0.0	3.948333	109673	110425	801-961	1.000000	60.1
ccsA-ndhD_p1	ccsA-ndhD	1	AGGCATTCTTTCTGGAGCAG	TTGCATTGCCAGGAATGAGT	20	20	50.0	45.0	57.9	58.1	0.0	15.1	0.198286	112410	112697	869-896	1.000000	85.7
ccsA-ndhD_p2	ccsA-ndhD	2	GGCATTCTTTCTGGAGCAGT	TTGCATTGCCAGGAATGAGT	20	20	50.0	45.0	58.2	58.1	0.0	15.1	0.240932	112410	112697	868-895	1.000000	85.4
ccsA-ndhD_p3	ccsA-ndhD	3	ATGCAGCTCTTTTATGCGGA	TTGCATTGCCAGGAATGAGT	20	20	45.0	45.0	58.0	58.1	0.0	15.1	0.115199	112410	112697	1140-1167	1.000000	85.1
ccsA-ndhD_p4	ccsA-ndhD	4	TGCAGCTCTTTTATGCGGAT	TTGCATTGCCAGGAATGAGT	20	20	45.0	45.0	58.0	58.1	0.0	15.1	0.115199	112410	112697	1139-1166	1.000000	85.1
ccsA-ndhD_p5	ccsA-ndhD	5	TTTTACCCAAGGTTTCGCCA	TTGCATTGCCAGGAATGAGT	20	20	45.0	45.0	57.9	58.1	0.0	15.1	0.141347	112410	112697	1259-1279	0.769231	75.2
ndhD_p1	ndhD	1	AGGCATTCTTTCTGGAGCAG	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.155611	112698	113861	1660-1979	1.000000	87.6
ndhD_p2	ndhD	2	GGCATTCTTTCTGGAGCAGT	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.198258	112698	113861	1659-1978	1.000000	87.3
ndhD_p3	ndhD	3	TCTGGAGCAGTATGGGCTAA	AGATGTGAATCCGCTTGTCC	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.204524	112698	113861	1650-1969	1.000000	87.2
ndhD_p4	ndhD	4	AGGCATTCTTTCTGGAGCAG	GCACGGGCTTTTCTGGTATA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.158454	112698	113861	1478-1797	0.846154	81.4
ndhD_p5	ndhD	5	GGCATTCTTTCTGGAGCAGT	GCACGGGCTTTTCTGGTATA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.201100	112698	113861	1477-1796	0.846154	81.0
psaC-ndhE_p1	psaC-ndhE	1	GTCCATCGGTTTCCAGTCTC	GTCGAAGCATGGCTAGAACA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021685	114237	114963	1462-1621	0.923077	86.1
psaC-ndhE_p2	psaC-ndhE	2	GTCCATCGGTTTCCAGTCTC	TCGAAGCATGGCTAGAACAC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.021685	114237	114963	1461-1620	0.923077	86.1
psaC-ndhE_p3	psaC-ndhE	3	GTCCATCGGTTTCCAGTCTC	CGAAGCATGGCTAGAACACT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.027276	114237	114963	1460-1619	0.923077	86.1
psaC-ndhE_p4	psaC-ndhE	4	GGACAAGCGGATTCACATCT	TCGAAGCATGGCTAGAACAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.039983	114237	114963	976-1138	0.923077	86.1
psaC-ndhE_p5	psaC-ndhE	5	GTCCATCGGTTTCCAGTCTC	ACAAGTCGAAGCATGGCTAG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.027276	114237	114963	1474-1625	0.769231	79.5